Cell-permeable (iCP)-SOCS3 recombinant protein and uses thereof

ABSTRACT

The present invention relates to providing improved cell-permeable (iCP)-SOCS3 recombinant protein and uses thereof. Preferably, the iCP-SOCS3 recombinant protein may be used as protein-based anti-pancreatic cancer agent by utilizing the platform technology for macromolecule intracellular transduction.

CROSS-REFERENCE TO RELATED APPLICATION

This application is a Continuation-in-Part of U.S. application Ser. No.14/838,288 (pending) filed on Aug. 27, 2015, which claims priority fromProvisional U.S. Application No. 62/042,493 filed on Aug. 27, 2014, thedisclosures of which are incorporated by reference in their entireties.

TECHNICAL FIELD

The present invention relates to providing improved cell-permeable(iCP)-SOCS3 recombinant protein and uses thereof. Preferably, theiCP-SOCS3 recombinant protein may be used as protein-basedanti-pancreatic cancer agent by utilizing the platform technology formacromolecule intracellular transduction.

BACKGROUND ART

Pancreatic cancer is the fifth leading causes of cancer-related death inthe world, but it is very aggressive disease and asymptomatic earlystages with a 5-year survival rate of less than 5%. There are so manyevidence of pancreatic cancer such as alcohol intake, high-fat diet,chronic pancreatitis, hereditary pancreatitis, obesity and type 2diabetes. Particularly, chronic inflammation plays a critical role inthe initiation and progression of early pancreatic lesions intopancreatic ductal adenocarcinoma (PDAC). Activation of signal transducerand activator of transcription 3 (STAT3) signaling has been observed inPDAC. Aberrant activation of Stat3 through homozygous deletion of SOCS3in the pancreas accelerates pancreatic intraepithelial neoplasiasprogression and PDAC development.

Cytokines including IL-6 and interferon-gamma (IFN-γ) activate the Januskinase (JAK)/STAT signaling pathway, a vital role promoting theinflammation, carcinogenesis and metastasis in the pancreas. Cytokinesignaling is strictly regulated by the SOCS family proteins induced bydifferent classes of agonists, including cytokines, hormones andinfectious agents. Among them, SOCS1 and SOCS3 are relatively specificto STAT1 and STAT3, respectively. SOCS1 inhibits JAK activation throughits N-terminal kinase inhibitory region (KIR) by the direct binding tothe activation loop of JAKs, while SOCS3 binds to janus kinases(JAKs)-proximal sites on the receptor through its SH2 domain andinhibits JAK activity that blocks recruitment of STAT3. Both promoteanti-inflammatory effects due to the suppression ofinflammation-inducing cytokine signaling. Furthermore, the SOCS box,another domain in SOCS proteins, interacts with E3 ubiquitin ligasesand/or couples the SH2 domain-binding proteins to theubiquitin-proteasome pathway. Therefore, SOCSs inhibit cytokinesignaling by suppressing JAK kinase activity and degrading the activatedcytokine receptor complex.

SOCS3 has been implicated as an anti-oncogene in the pancreatic cancerdevelopment. The methylation of SOCS3 gene has been reported to causethe non- or low-level of the protein expression in a number of humancancers. Previous studies have reported that aberrant methylation in theCpG island of SOCS3 induces its transcriptional silencing in pancreaticcancer and SOCS3 overexpression down-regulates active STAT3, inducesapoptosis and suppresses growth in cancer cells. This means that SOCS3plays a major role in the negative regulation of the JAK/STAT pathway incarcinogenesis and contributes to the suppression of pancreatic cancerdevelopment.

REFERENCES

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DISCLOSURE Technical Problem

To negatively control JAK/STAT signaling, recombinant SOCS3 proteinsthat contain a cell-penetrating peptide (CPP)-membrane-translocatingmotif (MTM) from fibroblast growth factor (FGF)-4 has been reported.These recombinant SOCS3 proteins inhibited STAT phosphorylation,inflammatory cytokines production and MHC-II expression in cultured andprimary macrophages. In addition, SOCS3 fused to MTM protected micechallenged with a lethal dose of the SEB super-antigen, by suppressingapoptosis and hemorrhagic necrosis in multiple organs. However, theSOCS3 proteins fused to FGF4-derived MTM displayed extremely lowsolubility, poor yields and relatively low cell- andtissue-permeability. Therefore, the MTM-fused SOCS3 proteins were notsuitable for further clinical development as therapeutic agents.

Technical Solution

For MITT, six critical factors (length, bending potential, instabilityindex, aliphatic index, GRAVY, amino acid composition) have beendetermined through analysis of baseline hydrophobic CPPs. Advancedmacromolecule transduction domain (aMTD), newly designed based on thesesix critical factors, could optimize cell-/tissue-permeability of SOCS3proteins that have a therapeutic effects and develop them asprotein-based drugs. Further, in order to increase solubility and yieldof recombinant protein, solubilization domains (SDs) additionally fusedto the aMTD-SOCS3 recombinant protein, thereby notably increased thesolubility and manufacturing yield of the recombinant protein.

In this application, aMTD/SD-fused iCP-SOCS3 recombinant proteins(iCP-SOCS3), much improved physicochemical characteristics (solubilityand yield) and functional activity (cell-/tissue-permeability) comparedwith the protein fused only to FGF-4-derived MTM. In addition, the newlydeveloped iCP-SOCS3 proteins have now been demonstrated to havetherapeutic application in treating pancreatic cancer, exploiting theability of SOCS3 to suppress JAK/STAT signaling. The present applicationrepresents that macromolecule intracellular transduction technology(MITT) enabled by the new hydrophobic CPPs that are aMTD may providenovel protein therapy through SOCS3-intracellular protein replacementagainst the pancreatic cancer. These findings suggest that restorationof SOCS3 by replenishing the intracellular SOCS3 with iCP-SOCS3 proteincreates a new paradigm for anti-cancer therapy, and the intracellularprotein replacement therapy with the SOCS3 recombinant protein fused tothe combination of aMTD and SD pair may be useful to treat thepancreatic cancer.

One aspect disclosed in the present application provides an improvedCell-Permeable (iCP)-SOCS3 recombinant protein, which comprises a SOCS3protein; and at least one advanced macromolecule transduction domain(aMTD)(s) being composed of 9-13 amino acid sequences and havingimproved cell and/or tissue permeability, wherein the aMTD(s) is fusedto one end or both ends of the SOCS3 protein and has the followingfeatures of:

(a) being composed of 3 or more amino acid sequences selected from thegroup consisting of Ala, Val, Ile, Leu, and Pro;

(b) having proline as amino acid sequences corresponding to any one ormore of positions 5 to 8, and 12 of its amino acid sequence; and

(c) having an instability index of 40-60; an aliphatic index of 180-220;and a grand average of hydropathy (GRAVY) of 2.1-2.6, as measured byProtparam.

According to one embodiment, the iCP-SOCS3 recombinant protein furthercomprises one or more solubilization domain (SD)(s), and the aMTD(s),SOCS3 protein and SD(s) may be randomly fused to one another.

According to another embodiment, the aMTD may form α-Helix structure.According to still another embodiment, the aMTD may be composed of 12amino acid sequences and represented by the following general formula:

wherein X(s) independently refer to Alanine (A), Valine (V), Leucine (L)or Isoleucine (I); and Proline (P) can be positioned in one of U(s)(either 5′, 6′, 7′ or 8′). The remaining U(s) are independently composedof A, V, L or I, P at the 12′ is Proline.

Another aspect disclosed in the present application provides aniCP-SOCS3 recombinant protein which is represented by any one of thefollowing structural formulae:

A-B-C, A-C-B, B-A-C, B-C-A, C-A-B, C-B-A, A-C-B-C and other possiblecombinations,

wherein A is an advanced macromolecule transduction domain (aMTD) havingimproved cell and/or tissue permeability, B is a SOCS3 protein, and C isa solubilization domain (SD); and

the aMTD is composed of 9-13 amino acid sequences and has the followingfeatures of:

(a) being composed of 3 or more amino acids selected from the groupconsisting of Ala, Val, Ile, Leu, and Pro;

(b) having proline as amino acid sequences corresponding to any one ormore of positions 5 to 8, and 12 of its amino acid sequence;

(c) having an instability index of 40-60; an aliphatic index of 180-220;and a grand average of hydropathy (GRAVY) of 2.1-2.6, as measured byProtparam; and

(d) forming α-Helix structure.

According to one embodiment disclosed in the present application, theSOCS3 protein may have an amino acid sequence of SEQ ID NO: 814.

According to another embodiment disclosed in the present application,the SOCS3 protein may be encoded by a polynucleotide sequence of SEQ IDNO: 815.

According to still another embodiment disclosed in the presentapplication, the SOCS3 protein may further include a ligand selectivelybinding to a receptor of a cell, a tissue, or an organ.

According to still another embodiment disclosed in the presentapplication, the at least one aMTD(s) may have an amino acid sequenceindependently selected from the group consisting of SEQ ID NOs: 1-240and 822.

According to still another embodiment disclosed in the presentapplication, the at least one aMTD(s) may be encoded by a polynucleotidesequence independently selected from the group consisting of SEQ ID NOs:241-480 and 823.

According to still another embodiment disclosed in the presentapplication, the one or more SD(s) may have an amino acid sequenceindependently selected from the group consisting of SEQ ID NOs: 798,799, 800, 801, 802, 803, and 804.

According to still another embodiment disclosed in the presentapplication, the one or more SD(s) may be encoded by a polynucleotidesequence independently selected from the group consisting of SEQ ID NOs:805, 806, 807, 808, 809, 810, and 811.

According to still another embodiment disclosed in the presentapplication, the iCP-SOCS3 recombinant protein may have a histidine-tagaffinity domain additionally fused to one end thereof.

According to still another embodiment disclosed in the presentapplication, the histidine-tag affinity domain may have an amino acidsequence of SEQ ID NO: 812.

According to still another embodiment disclosed in the presentapplication, the histidine-tag affinity domain may be encoded by apolynucleotide sequence of SEQ ID NO: 813.

According to still another embodiment disclosed in the presentapplication, the fusion may be formed via a peptide bond or a chemicalbond.

According to still another embodiment disclosed in the presentapplication, the iCP-SOCS3 recombinant protein may be used for thetreating, preventing, or delaying the onset of, pancreatic cancer.

Still another aspect disclosed in the present application provides apolynucleotide sequence encoding the iCP-SOCS3 recombinant protein.

According to one embodiment disclosed in the present application, thepolynucleotide sequence may be a polynucleotide sequence represented bySEQ ID NO: 825.

Still another aspect disclosed in the present application provides arecombinant expression vector including the polynucleotide sequence.

Still another aspect disclosed in the present application provides atransformant transformed with the recombinant expression vector.

Still another aspect disclosed in the present application provides apreparing method of the iCP-SOCS3 recombinant protein includingpreparing the recombinant expression vector; preparing the transformantusing the recombinant expression vector; culturing the transformant; andrecovering the recombinant protein expressed by the culturing.

Still another aspect disclosed in the present application provides acomposition including the iCP-SOCS3 recombinant protein as an activeingredient.

Still another aspect disclosed in the present application provides apharmaceutical composition for the treating, preventing, or delaying theonset of, pancreatic cancer including the iCP-SOCS3 recombinant proteinas an active ingredient; and a pharmaceutically acceptable carrier.

Still another aspect disclosed in the present application provides useof the iCP-SOCS3 recombinant protein as a medicament for the treating,preventing, or delaying the onset of, pancreatic cancer.

Still another aspect disclosed in the present application provides amedicament including the iCP-SOCS3 recombinant protein.

Still another aspect disclosed in the present application provides useof the iCP-SOCS3 recombinant protein in the preparation of a medicamentfor the treating, preventing, or delaying the onset of, pancreaticcancer.

Still another aspect disclosed in the present application provides amethod of treating, preventing, or delaying the onset of, pancreaticcancer in a subject, the method including identifying a subject in needof the treating, preventing, or delaying the onset of, pancreaticcancer; and administering to the subject a therapeutically effectiveamount of the iCP-SOCS3 recombinant protein.

According to one embodiment disclosed in the present application, thesubject may be a mammal.

Unless defined otherwise, all terms used herein have the same meaning ascommonly understood by one of ordinary skill in the art to which thepresent invention belongs. Although a certain method and a material isdescribed herein, it should not be construed as being limited thereto,any similar or equivalent method and material to those may also be usedin the practice or testing of the present invention. All publicationsmentioned herein are incorporated herein by reference in their entiretyto disclose and describe the methods and/or materials in connection withwhich the publications are cited. It must be noted that as used hereinand in the appended claims, the singular forms “a,” “an,” and “the”include plural referents unless the context clearly dictates otherwise.

A “peptide,” as used herein, refers to a chain-type polymer formed byamino acid residues which are linked to each other via peptide bonds,and used interchangeably with “polypeptide.” Further, a “polypeptide”includes a peptide and a protein.

Further, the term “peptide” includes amino acid sequences that areconservative variations of those peptides specifically exemplifiedherein. The term “conservative variation,” as used herein, denotes thereplacement of an amino acid residue by another, biologically similarresidue. Examples of conservative variations include substitution of onehydrophobic residue, such as isoleucine, valine, leucine, alanine,cysteine, glycine, phenylalanine, proline, tryptophan, tyrosine,norleucine, or methionine for another, or substitution of one polarresidue for another, for example, substitution of arginine for lysine,glutamic acid for aspartic acid, or glutamine for asparagine, and thelike. Neutral hydrophilic amino acids which may be substituted for oneanother include asparagine, glutamine, serine, and threonine.

The term “conservative variation” also includes use of a substitutedamino acid in place of an unsubstituted parent amino acid, provided thatantibodies raised to the substituted polypeptide also immunoreact withthe unsubstituted polypeptide. Such conservative substitutions arewithin the definition of the classes of the peptides disclosed in thepresent application.

A person having ordinary skill in the art may make similar substitutionsto obtain peptides having higher cell permeability and a broader hostrange. For example, one aspect disclosed in the present applicationprovides peptides corresponding to amino acid sequences (e.g. SEQ IDNOs: 1 to 240 and 822) provided herein, as well as analogues, homologs,isomers, derivatives, amidated variations, and conservative variationsthereof, as long as the cell permeability of the peptide remains.

Minor modifications to primary amino acid sequence disclosed in thepresent application may result in peptides which have substantiallyequivalent or enhanced cell permeability, as compared to the specificpeptides described herein. Such modifications may be deliberate, as bysite-directed mutagenesis, or may be spontaneous.

All peptides may be synthesized using L-amino acids, but D forms of allof the peptides may be synthetically produced. In addition, C-terminalderivatives, such as C-terminal methyl esters and C-terminal amidates,may be produced in order to increase the cell permeability of thepeptide according to one embodiment disclosed in the presentapplication.

All of the peptides produced by these modifications are included herein,as long as in the case of amidated versions of the peptide, the cellpermeability of the original peptide is altered or enhanced such thatthe amidated peptide is therapeutically useful. It is envisioned thatsuch modifications are useful for altering or enhancing cellpermeability of a particular peptide.

Furthermore, deletion of one or more amino acids may also result in amodification to the structure of the resultant molecule without anysignificant change in its cell permeability. This may lead to thedevelopment of a smaller active molecule which may also have utility.For example, amino- or carboxyl-terminal amino acids which may not berequired for the cell permeability of a particular peptide may beremoved.

The term “gene” refers to an arbitrary nucleic acid sequence or a partthereof having a functional role in protein coding or transcription, orregulation of other gene expression. The gene may be composed of allnucleic acids encoding a functional protein or a part of the nucleicacid encoding or expressing the protein. The nucleic acid sequence mayinclude a gene mutation in exon, intron, initiation or terminationregion, promoter sequence, other regulatory sequence, or a uniquesequence adjacent to the gene.

The term “primer” refers to an oligonucleotide sequence that hybridizesto a complementary RNA or DNA target polynucleotide and serves as thestarting points for the stepwise synthesis of a polynucleotide frommononucleotides by the action of a nucleotidyltransferase as occurs, forexample, in a polymerase chain reaction.

The term “coding region” or “coding sequence” refers to a nucleic acidsequence, a complement thereof, or a part thereof which encodes aparticular gene product or a fragment thereof for which expression isdesired, according to the normal base pairing and codon usagerelationships. Coding sequences include exons in genomic DNA or immatureprimary RNA transcripts, which are joined together by the cellularbiochemical machinery to provide a mature mRNA. The anti-sense strand isthe complement of the nucleic acid, and the coding sequence may bededuced therefrom.

One aspect disclosed in the present application provides an iCP-SOCS3recombinant protein, which comprises a SOCS3 protein and at least oneadvanced macromolecule transduction domain (aMTD)(s) being composed of9-13 amino acid sequences, preferably 10˜12 amino acid sequences, andhaving improved cell and/or tissue permeability, wherein the aMTD isfused to one end or both ends of the SOCS3 protein and has the followingfeatures of:

(a) being preferably composed of 3 or more amino acid sequences selectedfrom the group consisting of Ala, Val, Ile, Leu, and Pro;

(b) having proline as amino acid sequences corresponding to any one ormore of positions 5 to 8, and 12 of its amino acid sequence, andpreferably one or more of positions 5 to 8 and position 12 of its aminoacid sequence; and

(c) having an instability index of preferably 40-60 and more preferably41-58; an aliphatic index of preferably 180-220 and more preferably185-225; and a grand average of hydropathy (GRAVY) of preferably 2.1-2.6and more preferably 2.2-2.6 as measured by Protparam (seehttp://web.expasy.org/protparam/).

These critical factors that facilitate the cell permeable ability ofaMTD sequences were analyzed, identified, and determined according toone embodiment disclosed in the present application. These aMTDsequences are artificially assembled based on the critical factors (CFs)determined from in-depth analysis of previously published hydrophobicCPPs.

The aMTD sequences according to one aspect disclosed in the presentapplication are the first artificially developed cell permeablepolypeptides capable of mediating the transduction of biologicallyactive macromolecules—including peptides, polypeptides, protein domains,or full-length proteins—through the plasma membrane of cells.

According to one embodiment, the iCP-SOCS3 recombinant protein furthercomprises one or more solubilization domain (SD)(s), and the aMTD(s),SOCS3 protein and SD(s) may be randomly fused to one another. Forexample, SD(s) may be further fused to one or more of the SOCS3 proteinand the aMTD, preferably to one end or both ends of the SOCS3 protein,and more preferably to the C-terminus of the SOCS3 protein.

According to another embodiment, the aMTD may form α-Helix structure.

According to still another embodiment, the aMTD may be preferablycomposed of 12 amino acid sequences and represented by the followinggeneral formula:

Here, X(s) independently refer to Alanine (A), Valine (V), Leucine (L)or Isoleucine (I); and Proline (P) can be positioned in one of U(s)(either 5′, 6′, 7′ or 8′). The remaining U(s) are independently composedof A, V, L or I, P at the 12′ is Proline.

Still another aspect disclosed in the present application provides aniCP-SOCS3 recombinant protein which is represented by any one ofstructural formulae A-B-C, A-C-B, B-A-C, B-C-A, C-A-B, C-B-A, A-C-B-Cand other possible combinations, preferably by A-B-C or C-B-A:

wherein A is an advanced macromolecule transduction domain (aMTD) havingimproved cell and/or tissue permeability, and if the iCP-SOCS3recombinant protein comprises two or more aMTDs, they can be same ordifferent; B is a SOCS3 protein; and C is a solubilization domain (SD),and if the iCP-SOCS3 recombinant protein comprises two or more SDs, theycan be same or different; and

the aMTD is composed of 9-13, preferably 10˜12 amino acid sequences andhas the following features of:

(a) being composed of 3 or more amino acid sequences selected from thegroup consisting of Ala, Val, Ile, Leu, and Pro;

(b) having proline as amino acid sequences corresponding to any one ormore of positions 5 to 8, and 12 of its amino acid sequence, andpreferably, one or more of positions 5 to 8 and position 12 of its aminoacid sequence;

(c) having an instability index of 40-60, preferably 41-58 and morepreferably 50-58; an aliphatic index of 180-220. preferably 185-225 andmore preferably 195-205; and a grand average of hydropathy (GRAVY) of2.1-2.6 and preferably 2.2-2.6, as measured by Protparam (seehttp://web.expasy.org/protparam/); and

(d) preferably forming α-Helix structure.

In one embodiment disclosed in the present application, the SOCS3protein may have an amino acid sequence of SEQ ID NO: 814.

In another embodiment disclosed in the present application, the SOCS3protein may be encoded by a polynucleotide sequence of SEQ ID NO: 815.

When the iCP-SOCS3 recombinant protein is intended to be delivered to aparticular cell, tissue, or organ, the SOCS3 protein may form a fusionproduct, together with an extracellular domain of a ligand capable ofselectively binding to a receptor which is specifically expressed on theparticular cell, tissue, or organ, or monoclonal antibody (mAb) capableof specifically binding to the receptor or the ligand and a modifiedform thereof.

The binding of the peptide and a biologically active substance may beformed either by indirect linkage by a cloning technique using anexpression vector at a nucleotide level or by direct linkage viachemical or physical covalent or non-covalent bond of the peptide andthe biologically active substance.

In still another embodiment disclosed in the present application, theSOCS3 protein may preferably further include a ligand selectivelybinding to a receptor of a cell, a tissue, or an organ.

In one embodiment disclosed in the present application, the at least oneaMTD(s) may have an amino acid sequence independently selected from thegroup consisting of SEQ ID NOs: 1˜240 and 822, preferably SEQ ID NOs: 2,16, 22, 32, 40, 43, 63, 65, 77, 84, 85, 86, 110, 131, 142, 143, 177,228, 229, 233, 237, 239 and 822, more preferably SEQ ID NO: 43.

In still another embodiment disclosed in the present application, the atleast one aMTD(s) may be encoded by a polynucleotide sequenceindependently selected from the group consisting of SEQ ID NOs: 241˜480and 823, preferably SEQ ID NOs: 242, 256, 262, 272, 280, 283, 303, 305,317, 324, 325, 326, 350, 371, 382, 383, 417, 468, 469 473, 477, 479 and823, more preferably SEQ ID NO: 283.

In still another embodiment disclosed in the present application, theone or more SD(s) may have an amino acid sequence independently selectedfrom the group consisting of SEQ ID NOs: 798, 799, 800, 801, 802, 803,and 804. The SD may be preferably SDA of SEQ ID NO: 798, SDB of SEQ IDNO: 799, or SDB′ of SEQ ID NO: 804, and more preferably, SDB of SEQ IDNO: 799 which has superior structural stability, or SDB′ of SEQ ID NO:804 which has a modified amino acid sequence of SDB to avoid immuneresponses upon in vivo application. The modification of the amino acidsequence in SDB may be replacement of an amino acid residue, Valine,corresponding to position 28 of the amino acid sequence of SDB (SEQ IDNO: 799) by Leucine.

In still another embodiment disclosed in the present application, theone or more SDs may be encoded by a polynucleotide sequenceindependently selected from the group consisting of SEQ ID NOs: 805,806, 807, 808, 809, 810, and 811. The SD may be preferably SDA encodedby a polynucleotide sequence of SEQ ID NO: 805, SDB encoded by apolynucleotide sequence of SEQ ID NO: 806, or SDB′ for deimmunization(or humanization) encoded by a polynucleotide sequence of SEQ ID NO:811, and more preferably, SDB having superior structural stability,which is encoded by a polynucleotide sequence of SEQ ID NO: 806, or SDB′having a modified polynucleotide sequence of SDB to avoid immuneresponses upon in vivo application, which is encoded by a polynucleotidesequence of SEQ ID NO: 811.

In still another embodiment disclosed in the present application, theiCP-SOCS3 recombinant protein may be preferably selected from the groupconsisting of:

1) a recombinant protein, in which SOCS3 having an amino acid sequenceof SEQ ID NO: 814 is fused to the N-terminus or the C-terminus of aMTDhaving any one amino acid sequence selected from the group consisting ofSEQ ID NOs: 1 to 240 and 822, 2, 16, 22, 32, 40, 43, 63, 65, 77, 84, 85,86, 110, 131, 142, 143, 177, 228, 229, 233, 237, 239 and 822, morepreferably SEQ ID NO: 43;

2) a recombinant protein, in which SD having any one amino acid sequenceselected from the group consisting of SEQ ID NOs: 798 to 804 is furtherfused to one or more of the N-terminus or the C-terminus of the SOCS3and aMTD in the recombinant protein of 1); and

3) a recombinant protein, in which a Histidine tag having an amino acidsequence of 812 is further fused to the N-terminus of the recombinantprotein of 1) or 2).

According to one embodiment, the iCP-SOCS3 recombinant protein may becomposed of an amino acid sequence selected from the group consisting ofSEQ ID NO: 825.

The SOCS3 protein may exhibit a physiological phenomenon-relatedactivity or a therapeutic purpose-related activity by intracellular orin-vivo delivery. The recombinant expression vector may include a tagsequence which makes it easy to purify the recombinant protein, forexample, consecutive histidine codon, maltose binding protein codon, Myccodon, etc., and further include a fusion partner to enhance solubilityof the recombinant protein, etc. Further, for the overall structural andfunctional stability of the recombinant protein or flexibility of theproteins encoded by respective genes, the recombinant expression vectormay further include one or more glycine, proline, and spacer amino acidor polynucleotide sequences including AAY amino acids. Furthermore, therecombinant expression vector may include a sequence specificallydigested by an enzyme in order to remove an unnecessary region of therecombinant protein, an expression regulatory sequence, and a marker orreporter gene sequence to verify intracellular delivery, but is notlimited thereto.

In still another embodiment disclosed in the present application, theiCP-SOCS3 recombinant protein may preferably have a histidine-tagaffinity domain additionally fused to one end thereof.

In still another embodiment disclosed in the present application, thehistidine-tag affinity domain may have an amino acid sequence of SEQ IDNO: 812.

In still another embodiment disclosed in the present application, thehistidine-tag affinity domain may be encoded by a polynucleotidesequence of SEQ ID NO: 813.

In still another embodiment disclosed in the present application, thefusion may be formed via a peptide bond or a chemical bond.

The chemical bond may be preferably selected from the group consistingof disulfide bonds, diamine bonds, sulfide-amine bonds, carboxyl-aminebonds, ester bonds, and covalent bonds.

In still another embodiment disclosed in the present application, theiCP-SOCS3 recombinant protein may be used for the treating, preventing,or delaying the onset of, pancreatic cancer.

Still another aspect disclosed in the present application provides apolynucleotide sequence encoding the iCP-SOCS3.

According to still another embodiment disclosed in the presentapplication, the polynucleotide sequence may be fused with ahistidine-tag affinity domain.

Still another aspect disclosed in the present application provides arecombinant expression vector including the polynucleotide sequence.

Preferably, the vector may be inserted in a host cell and recombinedwith the host cell genome, or refers to any nucleic acid including anucleotide sequence competent to replicate spontaneously as an episome.Such a vector may include a linear nucleic acid, a plasmid, a phagemid,a cosmid, an RNA vector, a viral vector, etc.

Preferably, the vector may be genetically engineered to incorporate thenucleic acid sequence encoding the recombinant protein in an orientationeither N-terminal and/or C-terminal to a nucleic acid sequence encodinga peptide, a polypeptide, a protein domain, or a full-length protein ofinterest, and in the correct reading frame so that the recombinantprotein consisting of aMTD, SOCS3 protein, and preferably SD may beexpressed. Expression vectors may be selected from those readilyavailable for use in prokaryotic or eukaryotic expression systems.

Standard recombinant nucleic acid methods may be used to express agenetically engineered recombinant protein. The nucleic acid sequenceencoding the recombinant protein according to one embodiment disclosedin the present application may be cloned into a nucleic acid expressionvector, e.g., with appropriate signal and processing sequences andregulatory sequences for transcription and translation, and the proteinmay be synthesized using automated organic synthetic methods. Syntheticmethods of producing proteins are described in, for example, theliterature [Methods in Enzymology, Volume 289: Solid-Phase PeptideSynthesis by Gregg B. Fields (Editor), Sidney P. Colowick, Melvin I.Simon (Editor), Academic Press (1997)].

In order to obtain high level expression of a cloned gene or nucleicacid, for example, a cDNA encoding the recombinant protein according toone embodiment disclosed in the present application, the recombinantprotein sequence may be typically subcloned into an expression vectorthat includes a strong promoter for directing transcription, atranscription/translation terminator, and in the case of a nucleic acidencoding a protein, a ribosome binding site for translationalinitiation. Suitable bacterial promoters are well known in the art andare described, e.g., in the literatures [Sambrook & Russell, MolecularCloning: A Laboratory Manual, 3d Edition, Cold Spring Harbor Laboratory,N.Y. (2001); and Ausubel, et al., Current Protocols in MolecularBiology, Greene Publishing Associates and Wiley Interscience, N. Y.(1989)]. Bacterial expression systems for expression of the recombinantprotein are available in, e.g., E. coli, Bacillus sp., and Salmonella(Palva et al., Gene 22: 229-235 (1983); Mosbach et al., Nature 302:543-545 (1983)). Kits for such expression systems are commerciallyavailable. Eukaryotic expression systems for mammalian cells, yeast, andinsect cells are well known in the art and are also commerciallyavailable. The eukaryotic expression vector may be preferably anadenoviral vector, an adeno-associated vector, or a retroviral vector.

Generally, the expression vector for expressing the cell permeablerecombinant protein according to one embodiment disclosed in the presentapplication in which the cargo protein, i.e. ΔSOCS3 protein, is attachedto the N-terminus, C-terminus, or both termini of aMTD may includeregulatory sequences including, for example, a promoter, operablyattached to a sequence encoding the advanced macromolecule transductiondomain. Non-limiting examples of inducible promoters that may be usedinclude steroid-hormone responsive promoters (e.g., ecdysone-responsive,estrogen-responsive, and glutacorticoid-responsive promoters),tetracycline “Tet-On” and “Tet-Off” systems, and metal-responsivepromoters.

The polynucleotide sequence according to one embodiment disclosed in thepresent application may be present in a vector in which thepolynucleotide sequence is operably linked to regulatory sequencescapable of providing for the expression of the polynucleotide sequenceby a suitable host cell.

According to one embodiment disclosed in the present application, thepolynucleotide sequence may be selected from the following groups:

1) a polynucleotide sequence, in which any one polynucleotide sequenceselected from the group consisting of SEQ ID NOs: 241˜480 and 823,preferably SEQ ID NOs: 242, 252, 274, 279, 322, 331, 338, 345, 347, 361,365, 370, 371, 383, 387, 417, 462, 468, 469, 473, 477, 479 and 823, morepreferably SEQ ID NO: 283, is operably linked with a polynucleotidesequence of SEQ ID NO: 815; and

2) a polynucleotide sequence, in which any one polynucleotide sequenceselected from the group consisting of SEQ ID NOs: 805 to 811 is furtheroperably linked to the polynucleotide sequence of 1), or furtheroperably linked to between: any one polynucleotide sequence selectedfrom the group consisting of SEQ ID NOs: 241˜480 and 823, preferably SEQID NOs: 242, 256, 262, 272, 280, 283, 303, 305, 317, 324, 325, 326, 350,371, 382, 383, 417, 468, 469, 473, 477, 479 and 823, more preferably SEQID NO: 283; and a polynucleotide sequence of SEQ ID NO: 815.

Within an expression vector, the term “operably linked” is intended tomean that the polynucleotide sequence of interest is linked to theregulatory sequence(s) in a manner which allows for expression of thepolynucleotide sequence. The term “regulatory sequence” is intended toinclude promoters, enhancers, and other expression control elements.Such operable linkage with the expression vector can be achieved byconventional gene recombination techniques known in the art, whilesite-directed DNA cleavage and linkage are carried out by usingconventional enzymes known in the art.

The expression vectors may contain a signal sequence or a leadersequence for membrane targeting or secretion, as well as regulatorysequences such as a promoter, an operator, an initiation codon, atermination codon, a polyadenylation signal, an enhancer and the like.The promoter may be a constitutive or an inducible promoter. Further,the expression vector may include one or more selectable marker genesfor selecting the host cell containing the expression vector, and mayfurther include a polynucleotide sequence that enables the vector toreplicate in the host cell in question.

The expression vector constructed according to one embodiment disclosedin the present application may be the vector where the polynucleotideencoding the iCP-SOCS3 recombinant protein (where an aMTD is fused tothe N-terminus or C-terminus of a SOCS3 protein) is inserted within themultiple cloning sites (MCS), preferably within the Nde1/Sal1 site orBamH1/Sal1 site of a pET-28a(+)(Novagen, Darmstadt, Germany) orpET-26b(+) vector(Novagen, Darmstadt, Germany).

In still another embodiment disclosed in the present application, thepolynucleotide encoding the SD being additionally fused to theN-terminus or C-terminus of a SOCS3 protein or an aMTD may be insertedinto a cleavage site of restriction enzyme (Ndel, BamH1 and Sal1, etc.)within the multiple cloning sites (MCS) of a pET-28a(+)(Novagen,Darmstadt, Germany) or pET-26b(+) vector(Novagen, Darmstadt, Germany).

In still another embodiment disclosed in the present application, thepolynucleotide encoding the iCP-SOCS3 recombinant protein may be clonedinto a pET-28a(+) vector bearing a His-tag sequence so as to fuse sixhistidine residues to the N-terminus of the iCP-SOCS3 recombinantprotein to allow easy purification.

According to one embodiment disclosed in the present application, thepolynucleotide sequence may be a polynucleotide sequence selected fromthe group consisting of SEQ ID NOS: 824, 826, 828 and 830.

The recombinant protein may be introduced into an appropriate host cell,e.g., a bacterial cell, a yeast cell, an insect cell, or a tissueculture cell. The recombinant protein may also be introduced intoembryonic stem cells in order to generate a transgenic organism. Largenumbers of suitable vectors and promoters are known to those skilled inthe art and are commercially available for generating the recombinantprotein.

Known methods may be used to construct vectors including thepolynucleotide sequence according to one embodiment disclosed in thepresent application and appropriate transcriptional/translationalcontrol signals. These methods include in vitro recombinant DNAtechniques, synthetic techniques, and in vivo recombination/geneticrecombination. For example, these techniques are described in theliteratures [Sambrook & Russell, Molecular Cloning: A Laboratory Manual,3d Edition, Cold Spring Harbor Laboratory, N. Y. (2001); and Ausubel etal., Current Protocols in Molecular Biology Greene Publishing Associatesand Wiley Interscience, N.Y. (1989)].

Still another aspect disclosed in the present application provides atransformant transformed with the recombinant expression vector.

The transformation includes transfection, and refers to a processwhereby a foreign (extracellular) DNA, with or without an accompanyingmaterial, enters into a host cell. The “transfected cell” refers to acell into which the foreign DNA is introduced into the cell, and thusthe cell harbors the foreign DNA. The DNA may be introduced into thecell so that a nucleic acid thereof may be integrated into thechromosome or replicable as an extrachromosomal element. The cellintroduced with the foreign DNA, etc. is called a transformant.

As used herein, ‘introducing’ of a protein, a peptide, an organiccompound into a cell may be used interchangeably with the expression of‘carrying,’ ‘penetrating,’ ‘transporting,’ ‘delivering,’ ‘permeating’ or‘passing.’

It is understood that the host cell refers to a eukaryotic orprokaryotic cell into which one or more DNAs or vectors are introduced,and refers not only to the particular subject cell but also to theprogeny or potential progeny thereof. Because certain modifications mayoccur in succeeding generations due to either mutation or environmentalinfluences, such progeny may not, in fact, be identical to the parentcell, but are still included within the scope of the term as usedherein.

The host cells may be preferably bacterial cells, and as the bacterialcells, there are, in principle, no limitations. They may be eubacteria(gram-positive or gram-negative) or archaebacteria, as long as theyallow genetic manipulation for insertion of a gene of interest,preferably for site-specific integration, and they may be cultured on amanufacturing scale. Preferably, the host cells may have the property toallow cultivation to high cell densities.

Examples of bacterial host cells that may be used in the preparation ofthe recombinant protein are E. coli (Lee, 1996; Hannig and Makrides,1998), Bacillus subtilis, Pseudomonas fluorescens (Squires et al., 2004;Retallack et al., 2006) as well as various Corynebacterium (US2006/0003404 A1) and Lactococcus lactis (Mierau et al., 2005) strains.Preferably, the host cells are Escherichia coli cells.

More preferably, the host cell may include an RNA polymerase capable ofbinding to a promoter regulating the gene of interest. The RNApolymerase may be endogenous or exogenous to the host cell.

Preferably, host cells with a foreign strong RNA polymerase may be used.For example, Escherichia coli strains engineered to carry a foreign RNApolymerase (e.g. like in the case of using a T7 promoter a T7-like RNApolymerase in the so-called “T7 strains”) integrated in their genome maybe used. Examples of T7 strains, e.g. BL21(DE3), HMS174(DE3), and theirderivatives or relatives (see Novagen, pET System manual, 11^(th)edition), may be widely used and commercially available. Preferably,BL21-CodonPlus (DE3)-RIL or BL21-CodonPlus (DE3)-RIPL (AgilentTechnologies) may be used. These strains are DE3 lysogens containing theT7 RNA polymerase gene under control of the lacUV5 promoter. Inductionwith IPTG allows production of T7 RNA polymerase which then directs theexpression of the gene of interest under the control of the T7 promoter.

The host cell strains, E. coli BL21(DE3) or HMS174(DE3), which havereceived their genome-based T7 RNA polymerase via the phage DE3, arelysogenic. It is preferred that the T7 RNA polymerase contained in thehost cell has been integrated by a method which avoids, or preferablyexcludes, the insertion of residual phage sequences in the host cellgenome since lysogenic strains have the disadvantage to potentiallyexhibit lytic properties, leading to undesirable phage release and celllysis.

Still another aspect disclosed in the present application provides apreparing method of the iCP-SOCS3 recombinant protein includingpreparing the recombinant expression vector; preparing the transformantusing the recombinant expression vector; culturing the transformant; andrecovering the recombinant protein expressed by culturing.

Culturing may be preferably in a mode that employs the addition of afeed medium, this mode being selected from the fed-batch mode,semi-continuous mode, or continuous mode, and the bacterial expressionhost cells may include a DNA construct, integrated in their genome,carrying the DNA sequence encoding the protein of interest under thecontrol of a promoter that enables expression of said protein.

There are no limitations in the type of the culture medium. The culturemedium may be semi-defined, i.e. containing complex media compounds(e.g. yeast extract, soy peptone, casamino acids), or it may bechemically defined, without any complex compounds. Preferably, a definedmedium may be used. The defined media (also called minimal or syntheticmedia) are exclusively composed of chemically defined substances, i.e.carbon sources such as glucose or glycerol, salts, vitamins, and, inview of a possible strain auxotrophy, specific amino acids or othersubstances such as thiamine. Most preferably, glucose may be used as acarbon source. Usually, the carbon source of the feed medium serves asthe growth-limiting component which controls the specific growth rate.

Host cells may be disrupted by any convenient method, includingfreeze-thaw cycling, sonication, mechanical disruption, or the use ofcell lysing agents. The literature [Scopes, Protein Purification:Principles and Practice, New York: Springer-Verlag (1994)] describes anumber of general methods for purifying recombinant (andnon-recombinant) proteins. The methods may include, e.g., ion-exchangechromatography, size-exclusion chromatography, affinity chromatography,selective precipitation, dialysis, and hydrophobic interactionchromatography. These methods may be adapted to devise a purificationstrategy for the cell permeable recombinant protein. If the cellpermeable recombinant protein includes a purification handle, such as anepitope tag or a metal chelating sequence, affinity chromatography maybe used to easily purify the protein.

The amount of the protein produced may be evaluated by detecting theadvanced macromolecule transduction domain directly (e.g., using Westernanalysis) or indirectly (e.g., by assaying materials derived from thecells for specific DNA binding activity, such as by electrophoreticmobility shift assay). Proteins may be detected prior to purification,during any stage of purification, or after purification. In someimplementations, purification or complete purification may not benecessary.

The iCP-SOCS3 recombinant proteins according to one embodiment disclosedin the present application are cell permeable proteins, and may be usedas protein-based vaccines, particularly in the case where killed orattenuated whole organism vaccines are impractical.

The iCP-SOCS3 recombinant proteins according to one embodiment disclosedin the present application may be preferably used for the treating,preventing, or delaying the onset of, pancreatic cancer. The cellpermeable recombinant proteins may be delivered to the interior of thecell, eliminating the need to transfect or transform the cell with arecombinant vector. The cell permeable recombinant proteins may be usedin vitro to investigate protein function or may be used to maintaincells in a desired state.

Still another aspect disclosed in the present application provides acomposition including the iCP-SOCS3 Recombinant Protein as an activeingredient.

Still another aspect disclosed in the present application provides apharmaceutical composition for treating, preventing, or delaying theonset of, pancreatic cancer including the iCP-SOCS3 Recombinant Proteinas an active ingredient; and a pharmaceutically acceptable carrier.

According to one embodiment disclosed in the present application, theiCP-SOCS3 Recombinant Protein may be used in a single agent, or incombination with one or more other anti-cancer agents.

Pancreatic cancers described herein include, but are not limited to,exocrine pancreatic cancers including adenocarcinomas, acinar cellcarcinomas, adenosquamous carcinomas, colloid carcinomas,undifferentiated carcinomas with osteoclast-like giant cells,intraductal papillary-mucinous neoplasms, mucinous cystic neoplasms,pancreatoblastomas, serous cystadenomas, signet ring cell carcinomas,solid and pseuodpapiUary tumors, pancreatic ductal carcinomas, andundifferentiated carcinomas; and endocrine pancreatic cancers includinggastrinomas, insulinomas and glucagonomas.

According to one embodiment disclosed in the present invention, thepancreatic cancer may be early stage pancreatic cancer, metastaticpancreatic cancer, non-metastatic pancreatic cancer, primary pancreaticcancer, resected pancreatic cancer, advanced pancreatic cancer, locallyadvanced pancreatic cancer, unresectable pancreatic cancer, pancreaticcancer in remission, or recurrent pancreatic cancer.

Preferably, the composition may be for injectable (e.g. intraperitoneal,intravenous, and intra-arterial, etc.) and may include the activeingredient in an amount of 0.001 mg/kg to 1000 mg/kg, preferably 0.01mg/kg to 100 mg/kg, more preferably 0.1 mg/kg to 50 mg/kg for human.

For examples, dosages per day normally fall within the range of about0.001 to about 1000 mg/kg of body weight. In the treatment of adulthumans, the range of about 0.1 to about 50 mg/kg/day, in single ordivided dose, is especially preferred. However, it will be understoodthat the concentration of the iCP-SOCS3 recombinant protein actuallyadministered will be determined by a physician, in the light of therelevant circumstances, including the condition to be treated, thechosen route of administration, the age, weight, and response of theindividual patient, and the severity of the patient's symptoms, andtherefore the above dosage ranges are not intended to limit the scope ofthe invention in any way. In some instances dosage levels below thelower limit of the aforesaid range may be more than adequate, while inother cases still larger doses may be employed without causing anyharmful side effect, provided that such larger doses are first dividedinto several smaller doses for administration throughout the day.

Still another aspect disclosed in the present application provides useof the iCP-SOCS3 recombinant protein as a medicament for treating,preventing, or delaying the onset of, pancreatic cancer.

Still another aspect disclosed in the present application provides amedicament including the iCP-SOCS3 recombinant protein.

Still another aspect disclosed in the present application provides useof the iCP-SOCS3 recombinant protein for the preparation of a medicamentfor treating, preventing, or delaying the onset of, pancreatic cancer.

Still another aspect disclosed in the present application provides amethod of treating, preventing, or delaying the onset of, pancreaticcancer in a subject including identifying a subject in need of treating,preventing, or delaying the onset of, pancreatic cancer; andadministering to the subject a therapeutically effective amount of theiCP-SOCS3 recombinant protein.

In one embodiment disclosed in the present application, the subject maybe preferably a mammal.

Preferably, the subject in need of treating, preventing, or delaying theonset of, pancreatic cancer may be the people having symptoms such asabdominal pain, weight loss, itching, or jaundice (yellow skin), etc.

The pharmaceutical composition according to one embodiment disloced inthe present application may be prepared by using pharmaceuticallysuitable and physiologically acceptable additives, in addition to theactive ingredient, and the additives may include excipients,disintegrants, sweeteners, binders, coating agents, blowing agents,lubricants, glidants, flavoring agents, etc.

For administration, the pharmaceutical composition may be preferablyformulated by further including one or more pharmaceutically acceptablecarriers in addition to the above-described active ingredient.

Dosage forms of the pharmaceutical composition may include granules,powders, tablets, coated tablets, capsules, suppositories, liquidformulations, syrups, juice, suspensions, emulsions, drops, injectableliquid formulations, etc. For formulation of the composition into atablet or capsule, for example, the active ingredient may be combinedwith any oral, non-toxic pharmaceutically acceptable inert carrier, suchas ethanol, glycerol, water, etc. If desired or necessary, suitablebinders, lubricants, disintegrants, and colorants may be additionallyincluded as a mixture.

Examples of the suitable binder may include, but are not limited to,starch, gelatin, natural sugars such as glucose or beta-lactose, cornsweeteners, natural and synthetic gums such as acacia, tragacanth, orsodium oleate, sodium stearate, magnesium stearate, sodium benzoate,sodium acetate, sodium chloride, etc. Examples of the disintegrant mayinclude, but are not limited to, starch, methyl cellulose, agar,bentonite, xanthan gum, etc. For formulation of the composition into aliquid preparation, a pharmaceutically acceptable carrier which issterile and biocompatible may be used, such as saline, sterile water, aRinger's solution, buffered saline, an albumin infusion solution, adextrose solution, a maltodextrin solution, glycerol, and ethanol, andthese materials may be used alone or in any combination thereof. Ifnecessary, other common additives, such as antioxidants, buffers,bacteriostatic agents, etc., may be added. Further, diluents,dispersants, surfactants, binders, and lubricants may be additionallyadded to prepare injectable formulations such as aqueous solutions,suspensions, and emulsions, or pills, capsules, granules, or tablets.Furthermore, the composition may be preferably formulated, dependingupon diseases and ingredients, using any appropriate method known in theart, as disclosed in Remington's Pharmaceutical Science, Mack PublishingCompany, Easton Pa.

Preferably, the treatment or treating mean improving or stabilizing thesubject's condition or disease; or preventing or relieving thedevelopment or worsening of symptoms associated with the subject'scondition or disease. The prevention, prophylaxis and preventivetreatment are used herein as synonyms.

Preferably, the treating, preventing, or delaying the onset of,pancreatic cancer may be any one or more of the following: alleviatingone or more symptoms of pancreatic cancer, delaying progressing ofpancreatic cancer, shrinking tumor size in pancreatic cancer patient,inhibiting tumor growth, prolonging overall survival, prolongingdisease-free survival, prolonging time to pancreatic cancer progression,preventing or delaying metastasis, reducing or eradiating preexistingtumor metastasis, reducing incidence or burden of preexisting tumormetastasis, preventing recurrence of pancreatic cancer.

The subject and patient are used herein interchangeably. They refer to ahuman or another mammal (e.g., mouse, rat, rabbit, dog, cat, cattle,swine, sheep, horse or primate) that can be afflicted with or issusceptible to a disease or disorder but may or may not have the diseaseor disorder. In certain embodiments, the subject is a human being.

Preferably, the amount effective or effective amount is the amount of anactive ingredient or a pharmaceutical composition disclosed herein thatwhen administered to a subject for treating a disease, is sufficient toeffect such treatment of the disease. Any improvement in the patient isconsidered sufficient to achieve treatment. An effective amount of anactive ingredient or a pharmaceutical composition disclosed herein, usedfor the preventing, or delaying the onset of, pancreatic cancer may varydepending upon the manner of administration, the age, body weight, andgeneral health of the patient. Ultimately, the prescribers orresearchers will decide the appropriate amount and dosage regimen.

In the treatment or prevention method according to one embodimentdisclosed in the present application, the composition including theiCP-SOCS3 recombinant protein as an active ingredient may beadministered in a common manner via oral, buccal, rectal, intravenous,intra-arterial, intraperitoneal, intramuscular, intrasternal,percutaneous, topical, intraocular or subcutaneous route, morepreferably via intraperitoneal, intravenous, or intra-arterial injectionroute.

Advantageous Effects

According to one aspect disclosed in the present application,development and establishment of improved cell-permeable SOCS3recombinant protein, as therapeutics of pancreatic cancer are provided.Because iCP-SOCS3 was designed based on endogenous proteins, it would bea safety anti-pancreatic cancer drug without side-effect.

However, the effects of the disclosures in the present application arenot limited to the above-mentioned effects, and another effects notmentioned will be clearly understood by those skilled in the art fromthe following description.

DESCRIPTION OF DRAWINGS

FIG. 1 shows Structure of aMTD- or rPeptide-Fused Recombinant Proteins.A schematic diagram of the His-tagged CRA recombinant proteins isillustrated and constructed according to the present invention. Thehis-tag for affinity purification (white), aMTD or rPeptide (gray) andcargo A (CRA, black) are shown.

FIG. 2a shows Construction of Expression Vectors for aMTDs- orrPeptide-Fused Recombinant Proteins.

FIGS. 2b and 2c show the agarose gel electrophoresis analysis showingplasmid DNA fragments at 645 bp insert encoding aMTDs or rPeptide-fusedCRA cloned into the pET28a(+) vector according to the present invention.In FIG. 2b , rP38 has the sequence of SEQ ID NO: 952, MTM12 has thesequence of SEQ ID NO: 953, MTD85 has the sequence of SEQ ID NO: 954,aMTD1 has the sequence of SEQ ID NO: 1, aMTD2 has the sequence of SEQ IDNO: 2, aMTD3 has the sequence of SEQ ID NO: 3, aMTD4 has the sequence ofSEQ ID NO:4, aMTD5 has the sequence of SEQ ID NO: 5, aMTD11 has thesequence of SEQ ID NO: 6, aMTD12 has the sequence of SEQ ID NO: 7,aMTD13 has the sequence of SEQ ID NO: 8, aMTD21 has the sequence of SEQID NO: 9, aMTD22 has the sequence of SEQ ID NO: 10, aMTD23 has thesequence of SEQ ID NO: 11, aMTD24 has the sequence of SEQ ID NO: 12,aMTD25 has the sequence of SEQ ID NO: 13, aMTD42 has the sequence of SEQID NO: 14, aMTD43 has the sequence of SEQ ID NO: 15, aMTD44 has thesequence of SEQ ID NO: 16, aMTD61 has the sequence of SEQ ID NO: 17,aMTD62 has the sequence of SEQ ID NO: 18, aMTD63 has the sequence of SEQID NO: 19, aMTD64 has the sequence of SEQ ID NO: 20, aMTD65 has thesequence of SEQ ID NO: 21, aMTD81 has the sequence of SEQ ID NO: 22,aMTD82 has the sequence of SEQ ID NO: 23, aMTD83 has the sequence of SEQID NO: 24, aMTD84 has the sequence of SEQ ID NO: 25, aMTD85 has thesequence of SEQ ID NO: 26, aMTD101 has the sequence of SEQ ID NO: 27,aMTD102 has the sequence of SEQ ID NO: 28, aMTD103 has the sequence ofSEQ ID NO: 29, aMTD104 has the sequence of SEQ ID NO: 30, aMTD105 hasthe sequence of SEQ ID NO: 31, aMTD121 has the sequence of SEQ ID NO:32, aMTD123 has the sequence of SEQ ID NO: 33, aMTD124 has the sequenceof SEQ ID NO: 34, aMTD141 has the sequence of SEQ ID NO: 35, aMTD143 hasthe sequence of SEQ ID NO: 36, aMTD144 has the sequence of SEQ ID NO:37, aMTD145 has the sequence of SEQ ID NO: 38, aMTD161 has the sequenceof SEQ ID NO: 39, aMTD162 has the sequence of SEQ ID NO: 40, aMTD163 hasthe sequence of SEQ ID NO: 41, aMTD164 has the sequence of SEQ ID NO:42, aMTD165 has the sequence of SEQ ID NO: 43, aMTD182 has the sequenceof SEQ ID NO: 44, aMTD183 has the sequence of SEQ ID NO: 45, aMTD184 hasthe sequence of SEQ ID NO: 46, aMTD185 has the sequence of SEQ ID NO:47, aMTD 201 has the sequence of SEQ ID NO: 48, aMTD204 has the sequenceof SEQ ID NO: 49, aMTD205 has the sequence of SEQ ID NO: 50, aMTD221 hasthe sequence of SEQ ID NO: 51, aMTD222 has the sequence of SEQ ID NO:52, aMTD223 has the sequence of SEQ ID NO: 53, aMTD224 has the sequenceof SEQ ID NO: 54, aMTD225 has the sequence of SEQ ID NO: 55, aMTD241 hasthe sequence of SEQ ID NO: 56, aMTD242 has the sequence of SEQ ID NO:57, aMTD243 has the sequence of SEQ ID NO: 58, aMTD245 has the sequenceof SEQ ID NO: 59, aMTD261 has the sequence of SEQ ID NO: 60, aMTD262 hasthe sequence of SEQ ID NO: 61, aMTD263 has the sequence of SEQ ID NO:62, aMTD264 has the sequence of SEQ ID NO: 63, aMTD265 has the sequenceof SEQ ID NO: 64, aMTD281 has the sequence of SEQ ID NO: 65, aMTD282 hasthe sequence of SEQ ID NO: 66, aMTD283 has the sequence of SEQ ID NO:67, aMTD284 has the sequence of SEQ ID NO: 68, aMTD285 has the sequenceof SEQ ID NO: 69, aMTD301 has the sequence of SEQ ID NO: 70, aMTD302 hasthe sequence of SEQ ID NO: 71, aMTD304 has the sequence of SEQ ID NO:72, aMTD305 has the sequence of SEQ ID NO: 73, aMTD321 has the sequenceof SEQ ID NO: 74, aMTD322 has the sequence of SEQ ID NO: 75, aMTD323 hasthe sequence of SEQ ID NO: 76, aMTD324 has the sequence of SEQ ID NO:77, aMTD325 has the sequence of SEQ ID NO: 78, aMTD341 has the sequenceof SEQ ID NO: 79, aMTD342 has the sequence of SEQ ID NO: 80, aMTD343 hasthe sequence of SEQ ID NO: 81, aMTD345 has the sequence of SEQ ID NO:82, aMTD361 has the sequence of SEQ ID NO: 83, aMTD363 has the sequenceof SEQ ID NO: 84, aMTD381 has the sequence of SEQ ID NO: 87, aMTD382 hasthe sequence of SEQ ID NO: 88, aMTD383 has the sequence of SEQ ID NO:89, aMTD384 has the sequence of SEQ ID NO: 90, aMTD385 has the sequenceof SEQ ID NO: 91, aMTD401 has the sequence of SEQ ID NO: 92, aMTD402 hasthe sequence of SEQ ID NO: 93, aMTD403 has the sequence of SEQ ID NO:94, aMTD404 has the sequence of SEQ ID NO: 95, aMTD405 has the sequenceof SEQ ID NO: 96, aMTD421 has the sequence of SEQ ID NO: 97, aMTD422 hasthe sequence of SEQ ID NO: 98, aMTD424 has the sequence of SEQ ID NO:99, aMTD425 has the sequence of SEQ ID NO: 100, aMTD442 has the sequenceof SEQ ID NO: 101, aMTD443 has the sequence of SEQ ID NO: 102, aMTD444has the sequence of SEQ ID NO: 103, aMTD445 has the sequence of SEQ IDNO: 104, aMTD461 has the sequence of SEQ ID NO: 105, aMTD462 has thesequence of SEQ ID NO: 106, aMTD463 has the sequence of SEQ ID NO: 107,aMTD464 has the sequence of SEQ ID NO: 108, aMTD465 has the sequence ofSEQ ID NO: 109, aMTD481 has the sequence of SEQ ID NO: 110, aMTD482 hasthe sequence of SEQ ID NO: 111, aMTD483 has the sequence of SEQ ID NO:112, aMTD484 has the sequence of SEQ ID NO: 113, aMTD485 has thesequence of SEQ ID NO: 114, aMTD501 has the sequence of SEQ ID NO: 115,aMTD502 has the sequence of SEQ ID NO: 116, aMTD503 has the sequence ofSEQ ID NO: 117, aMTD504 has the sequence of SEQ ID NO: 118, aMTD505 hasthe sequence of SEQ ID NO: 119, aMTD521 has the sequence of SEQ ID NO:120, aMTD522 has the sequence of SEQ ID NO: 121, aMTD524 has thesequence of SEQ ID NO: 122, aMTD525 has the sequence of SEQ ID NO: 123,aMTD541 has the sequence of SEQ ID NO: 124, aMTD542 has the sequence ofSEQ ID NO: 125, aMTD543 has the sequence of SEQ ID NO: 126, aMTD544 hasthe sequence of SEQ ID NO: 127, aMTD545 has the sequence of SEQ ID NO:128, aMTD561 has the sequence of SEQ ID NO: 129, aMTD562 has thesequence of SEQ ID NO: 130, aMTD563 has the sequence of SEQ ID NO: 131,aMTD564 has the sequence of SEQ ID NO: 132, aMTD565 has the sequence ofSEQ ID NO: 133, aMTD582 has the sequence of SEQ ID NO: 134, aMTD583 hasthe sequence of SEQ ID NO: 135, aMTD585 has the sequence of SEQ ID NO:136, aMTD601 has the sequence of SEQ ID NO: 137, aMTD602 has thesequence of SEQ ID NO: 138, aMTD603 has the sequence of SEQ ID NO: 139,aMTD604 has the sequence of SEQ ID NO: 140, aMTD605 has the sequence ofSEQ ID NO: 141, aMTD622 has the sequence of SEQ ID NO: 142, aMTD623 hasthe sequence of SEQ ID NO: 143, aMTD625 has the sequence of SEQ ID NO:144, aMTD643 has the sequence of SEQ ID NO: 145, aMTD645 has thesequence of SEQ ID NO: 146, aMTD661 has the sequence of SEQ ID NO: 147,aMTD664 has the sequence of SEQ ID NO: 148, aMTD665 has the sequence ofSEQ ID NO: 149, aMTD666 has the sequence of SEQ ID NO: 150, aMTD667 hasthe sequence of SEQ ID NO: 151, aMTD683 has the sequence of SEQ ID NO:152, aMTD684 has the sequence of SEQ ID NO:153, aMTD685 has the sequenceof SEQ ID NO: 154, aMTD686 has the sequence of SEQ ID NO: 155, aMTD687has the sequence of SEQ ID NO: 156, aMTD703 has the sequence of SEQ IDNO: 157, aMTD705 has the sequence of SEQ ID NO: 158, aMTD706 has thesequence of SEQ ID NO: 159, aMTD707 has the sequence of SEQ ID NO: 160,aMTD724 has the sequence of SEQ ID NO: 161, aMTD725 has the sequence ofSEQ ID NO: 162, aMTD726 has the sequence of SEQ ID NO: 163, aMTD727 hasthe sequence of SEQ ID NO: 164, aMTD743 has the sequence of SEQ ID NO:165, aMTD744 has the sequence of SEQ ID NO: 166, aMTD746 has thesequence of SEQ ID NO: 167, aMTD747 has the sequence of SEQ ID NO: 168,aMTD763 has the sequence of SEQ ID NO: 169, aMTD764 has the sequence ofSEQ ID NO: 170, aMTD765 has the sequence of SEQ ID NO: 171, aMTD766 hasthe sequence of SEQ ID NO: 172, aMTD767 has the sequence of SEQ ID NO:173, aMTD783 has the sequence of SEQ ID NO: 174, aMTD786 has thesequence of SEQ ID NO: 176, aMTD787 has the sequence of SEQ ID NO: 177,aMTD788 has the sequence of SEQ ID NO: 178, aMTD803 has the sequence ofSEQ ID NO: 179, aMTD805 has the sequence of SEQ ID NO: 180, aMTD806 hasthe sequence of SEQ ID NO: 181, aMTD807 has the sequence of SEQ ID NO:182, aMTD808 has the sequence of SEQ ID NO: 183, aMTD809 has thesequence of SEQ ID NO: 184, aMTD810 has the sequence of SEQ ID NO: 185,aMTD811 has the sequence of SEQ ID NO: 186.

In FIG. 2c , aMTD824 has the sequence of SEQ ID NO: 187, aMTD825 has thesequence of SEQ ID NO: 188, aMTD826 has the sequence of SEQ ID NO: 189,aMTD827 has the sequence of SEQ ID NO: 190, aMTD828 has the sequence ofSEQ ID NO: 191, aMTD829 has the sequence of SEQ ID NO: 192, aMTD830 hasthe sequence of SEQ ID NO: 193, aMTD831 has the sequence of SEQ ID NO:194, aMTD832 has the sequence of SEQ ID NO: 195, aMTD843 has thesequence of SEQ ID NO: 196, aMTD844 has the sequence of SEQ ID NO: 197,aMTD845 has the sequence of SEQ ID NO: 198, aMTD846 has the sequence ofSEQ ID NO: 199, aMTD847 has the sequence of SEQ ID NO: 200, aMTD848 hasthe sequence of SEQ ID NO: 201, aMTD849 has the sequence of SEQ ID NO:202, aMTD850 has the sequence of SEQ ID NO: 203, aMTD851 has thesequence of SEQ ID NO: 204, aMTD852 has the sequence of SEQ ID NO: 205,aMTD863 has the sequence of SEQ ID NO: 206, aMTD864 has the sequence ofSEQ ID NO: 207, aMTD865 has the sequence of SEQ ID NO: 208, aMTD867 hasthe sequence of SEQ ID NO: 209, aMTD868 has the sequence of SEQ ID NO:210, aMTD870 has the sequence of SEQ ID NO: 211, aMTD872 has thesequence of SEQ ID NO: 212, aMTD875 has the sequence of SEQ ID NO: 213,aMTD877 has the sequence of SEQ ID NO: 214, aMTD878 has the sequence ofSEQ ID NO: 215, aMTD879 has the sequence of SEQ ID NO: 216, aMTD881 hasthe sequence of SEQ ID NO: 217, aMTD882 has the sequence of SEQ ID NO:218, aMTD883 has the sequence of SEQ ID NO: 219, aMTD885 has thesequence of SEQ ID NO: 220, aMTD887 has the sequence of SEQ ID NO: 221,aMTD888 has the sequence of SEQ ID NO: 222, aMTD889 has the sequence ofSEQ ID NO: 223, aMTD891 has the sequence of SEQ ID NO: 224, aMTD893 hasthe sequence of SEQ ID NO: 225, aMTD895 has the sequence of SEQ ID NO:226, aMTD896 has the sequence of SEQ ID NO: 227, aMTD897 has thesequence of SEQ ID NO: 228, aMTD899 has the sequence of SEQ ID NO: 229,aMTD900 has the sequence of SEQ ID NO: 230, aMTD901 has the sequence ofSEQ ID NO: 231, aMTD902 has the sequence of SEQ ID NO: 232, aMTD904 hasthe sequence of SEQ ID NO: 233, aMTD905 has the sequence of SEQ ID NO:234, aMTD906 has the sequence of SEQ ID NO: 235, aMTD907 has thesequence of SEQ ID NO: 236, aMTD908 has the sequence of SEQ ID NO: 237,aMTD910 has the sequence of SEQ ID NO: 238, aMTD911 has the sequence ofSEQ ID NO: 239, aMTD912 has the sequence of SEQ ID NO: 240, rP38 has thesequence of SEQ ID NO: 952, MTM12 has the sequence of SEQ ID NO: 953,MTD85 has the sequence of SEQ ID NO: 954, rP6 has the sequence of SEQ IDNO: 893, rP9 has the sequence of SEQ ID NO: 912, rP16 has the sequenceof SEQ ID NO: 890, rP17 has the sequence of SEQ ID NO: 905, rP18 has thesequence of SEQ ID NO: 945, rP19 has the sequence of SEQ ID NO: 942,rP20 has the sequence of SEQ ID NO: 946, rP26 has the sequence of SEQ IDNO: 895, rP27 has the sequence of SEQ ID NO: 885, rP28 has the sequenceof SEQ ID NO: 921, rP29 has the sequence of SEQ ID NO: 924, rP30 has thesequence of SEQ ID NO: 926, rP33 has the sequence of SEQ ID NO: 927,rP37 has the sequence of SEQ ID NO: 889, rP39 has the sequence of SEQ IDNO: 941, rP40 has the sequence of SEQ ID NO: 948, rP49 has the sequenceof SEQ ID NO: 918, rP54 has the sequence of SEQ ID NO: 939, rP57 has thesequence of SEQ ID NO: 949, rP59 has the sequence of SEQ ID NO: 938,rP66 has the sequence of SEQ ID NO: 902, rP67 has the sequence of SEQ IDNO: 906, rP68 has the sequence of SEQ ID NO: 913, rP69 has the sequenceof SEQ ID NO: 908, rP71 has the sequence of SEQ ID NO: 930, rP77 has thesequence of SEQ ID NO: 934, rP97 has the sequence of SEQ ID NO: 936,rP113 has the sequence of SEQ ID NO: 891, rP131 has the sequence of SEQID NO: 928, rP138 has the sequence of SEQ ID NO: 933, rP139 has thesequence of SEQ ID NO: 944, rP152 has the sequence of SEQ ID NO: 884,rP159 has the sequence of SEQ ID NO: 950, rP167 has the sequence of SEQID NO: 898, rP169 has the sequence of SEQ ID NO: 923, rP190 has thesequence of SEQ ID NO: 925, rP209 has the sequence of SEQ ID NO: rP210has the sequence of SEQ ID NO: 920, rP214 has the sequence of SEQ ID NO:937, rP226 has the sequence of SEQ ID NO: 892, rP227 has the sequence ofSEQ ID NO: 904, rP246 has the sequence of SEQ ID NO: 899, rP248 has thesequence of SEQ ID NO: 903, rP329 has the sequence of SEQ ID NO: 917,rP331 has the sequence of SEQ ID NO: 911, rP349 has the sequence of SEQID NO: 914, rP350 has the sequence of SEQ ID NO: 910, rP390 has thesequence of SEQ ID NO: 909, rP426 has the sequence of SEQ ID NO: 900,rP436 has the sequence of SEQ ID NO: 932, rP466 has the sequence of SEQID NO: 897, rP527 has the sequence of SEQ ID NO: 896, rP577 has thesequence of SEQ ID NO: 935, rP606 has the sequence of SEQ ID NO: 901,rP635 has the sequence of SEQ ID NO: 947, rP692 has the sequence of SEQID NO: 907, rP693 has the sequence of SEQ ID NO: 922, rP700 has thesequence of SEQ ID NO: 951, rP750 has the sequence of SEQ ID NO: 894,rP772 has the sequence of SEQ ID NO: 919, rP921 has the sequence of SEQID NO: 931, rP922 has the sequence of SEQ ID NO: 929, rP934 has thesequence of SEQ ID NO: 888, rP935 has the sequence of SEQ ID NO: 886,rP936 has the sequence of SEQ ID NO: 883, rP937 has the sequence of SEQID NO: 915, rP938 has the sequence of SEQ ID NO: 916, rP947 has thesequence of SEQ ID NO: 943, rP949 has the sequence of SEQ ID NO: 940.

FIGS. 3a to 3d show Inducible Expression of aMTD- or rPeptide-FusedRecombinant Proteins. Expressed recombinant aMTD- or randompeptide-fused CRA recombinant proteins were transformed in E. coli BL21(DE3) strain. Expression of recombinant proteins in E. coli before (−)and after (+) induction with IPTG was monitored by SDS-PAGE, and stainedwith Coomassie blue.

In FIG. 3a , MTM12 has the sequence of SEQ ID NO: 953, MTM16 has thesequence of SEQ ID NO: 865, MTD85 has the sequence of SEQ ID NO: 954,rP38 has the sequence of SEQ ID NO: 952, SDA has the sequence of SEQ IDNO: 798, MTD73 has the sequence of SEQ ID NO: 871, MTD103 has thesequence of SEQ ID NO: 876, aMTD562 has the sequence of SEQ ID NO: 130,aMTD563 has the sequence of SEQ ID NO: 131, aMTD564 has the sequence ofSEQ ID NO: 132, aMTD542 has the sequence of SEQ ID NO: 125, aMTD543 hasthe sequence of SEQ ID NO: 126, aMTD322 has the sequence of SEQ ID NO:75, aMTD323 has the sequence of SEQ ID NO: 76, aMTD403 has the sequenceof SEQ ID NO: 94, aMTD405 has the sequence of SEQ ID NO: 96, aMTD421 hasthe sequence of SEQ ID NO: 337, aMTD422 has the sequence of SEQ ID NO:98, aMTD443 has the sequence of SEQ ID NO: 102, aMTD445 has the sequenceof SEQ ID NO: 104, aMTD461 has the sequence of SEQ ID NO: 105, aMTD462has the sequence of SEQ ID NO: 106, aMTD363 has the sequence of SEQ IDNO: 84, aMTD364 has the sequence of SEQ ID NO: 986, aMTD365 has thesequence of SEQ ID NO: 86, aMTD424 has the sequence of SEQ ID NO: 99,aMTD442 has the sequence of SEQ ID NO: 101, aMTD603 has the sequence ofSEQ ID NO: 139, aMTD625 has the sequence of SEQ ID NO: 144, aMTD381 hasthe sequence of SEQ ID NO: 87, aMTD383 has the sequence of SEQ ID NO:89, aMTD384 has the sequence of SEQ ID NO: 90, aMTD385 has the sequenceof SEQ ID NO: 91, aMTD402 has the sequence of SEQ ID NO: 93, aMTD582 hasthe sequence of SEQ ID NO: 134, aMTD601 has the sequence of SEQ ID NO:137, aMTD902 has the sequence of SEQ ID NO: 232, aMTD875 has thesequence of SEQ ID NO: 213, aMTD877 has the sequence of SEQ ID NO: 214,aMTD345 has the sequence of SEQ ID NO: 82, aMTD163 has the sequence ofSEQ ID NO: 41, aMTD61 has the sequence of SEQ ID NO: 17, aMTD62 has thesequence of SEQ ID NO: 18, aMTD63 has the sequence of SEQ ID NO: 19,aMTD64 has the sequence of SEQ ID NO: 20, aMTD65 has the sequence of SEQID NO: 21, aMTD504 has the sequence of SEQ ID NO: 118, aMTD42 has thesequence of SEQ ID NO: 14, aMTD81 has the sequence of SEQ ID NO: 22,aMTD82 has the sequence of SEQ ID NO: 23, aMTD83 has the sequence of SEQID NO: 24, aMTD84 has the sequence of SEQ ID NO: 25, aMTD124 has thesequence of SEQ ID NO: 34, aMTD145 has the sequence of SEQ ID NO: 38,aMTD161 has the sequence of SEQ ID NO: 39, aMTD182 has the sequence ofSEQ ID NO: 44, aMTD204 has the sequence of SEQ ID NO: 49, aMTD205 hasthe sequence of SEQ ID NO: 50, aMTD324 has the sequence of SEQ ID NO:77, aMTD225 has the sequence of SEQ ID NO: 55, aMTD263 has the sequenceof SEQ ID NO: 62, aMTD643 has the sequence of SEQ ID NO: 145, aMTD1 hasthe sequence of SEQ ID NO: 1, aMTD2 has the sequence of SEQ ID NO: 2,aMTD3 has the sequence of SEQ ID NO: 3, aMTD4 has the sequence of SEQ IDNO: 4, aMTD5 has the sequence of SEQ ID NO: 5, aMTD325 has the sequenceof SEQ ID NO: 78, aMTD382 has the sequence of SEQ ID NO: 88, aMTD425 hasthe sequence of SEQ ID NO: 100, aMTD482 has the sequence of SEQ ID NO:111, aMTD401 has the sequence of SEQ ID NO: 92, aMTD444 has the sequenceof SEQ ID NO: 103, aMTD464 has the sequence of SEQ ID NO: 108, aMTD485has the sequence of SEQ ID NO: 114, aMTD501 has the sequence of SEQ IDNO: 115, aMTD505 has the sequence of SEQ ID NO: 119, aMTD524 has thesequence of SEQ ID NO: 122, aMTD525 has the sequence of SEQ ID NO: 123,aMTD683 has the sequence of SEQ ID NO: 152, aMTD684 has the sequence ofSEQ ID NO: 153, aMTD685 has the sequence of SEQ ID NO: 154, aMTD864 hasthe sequence of SEQ ID NO: 207, aMTD85 has the sequence of SEQ ID NO:26, aMTD102 has the sequence of SEQ ID NO: 28, aMTD103 has the sequenceof SEQ ID NO: 29, aMTD104 has the sequence of SEQ ID NO: 30, aMTD105 hasthe sequence of SEQ ID NO: 31, aMTD121 has the sequence of SEQ ID NO:32, aMTD165 has the sequence of SEQ ID NO: 43, aMTD12 has the sequenceof SEQ ID NO: 7, aMTD21 has the sequence of SEQ ID NO: 9, aMTD22 has thesequence of SEQ ID NO: 10, aMTD23 has the sequence of SEQ ID NO: 11,aMTD25 has the sequence of SEQ ID NO: 13, aMTD43 has the sequence of SEQID NO: 15, aMTD44 has the sequence of SEQ ID NO: 16, aMTD321 has thesequence of SEQ ID NO: 74, aMTD661 has the sequence of SEQ ID NO: 147,aMTD243 has the sequence of SEQ ID NO: 58, aMTD245 has the sequence ofSEQ ID NO: 59, aMTD264 has the sequence of SEQ ID NO: 63, aMTD282 hasthe sequence of SEQ ID NO: 66, aMTD602 has the sequence of SEQ ID NO:138, aMTD604 has the sequence of SEQ ID NO: 140.

In FIG. 3b , aMTD284 has the sequence of SEQ ID NO: 68, aMTD285 has thesequence of SEQ ID NO: 69, aMTD141 has the sequence of SEQ ID NO: 35,aMTD201 has the sequence of SEQ ID NO: 48, aMTD265 has the sequence ofSEQ ID NO: 64, aMTD281 has the sequence of SEQ ID NO: 65, aMTD304 hasthe sequence of SEQ ID NO: 72, aMTD305 has the sequence of SEQ ID NO:73, aMTD343 has the sequence of SEQ ID NO: 81, aMTD341 has the sequenceof SEQ ID NO: 79, aMTD301 has the sequence of SEQ ID NO: 70, aMTD522 hasthe sequence of SEQ ID NO: 121, aMTD645 has the sequence of SEQ ID NO:146, aMTD746 has the sequence of SEQ ID NO: 167, aMTD747 has thesequence of SEQ ID NO: 168, aMTD763 has the sequence of SEQ ID NO: 169,aMTD846 has the sequence of SEQ ID NO: 199, aMTD849 has the sequence ofSEQ ID NO: 202, aMTD101 has the sequence of SEQ ID NO: 27, aMTD185 hasthe sequence of SEQ ID NO: 47, aMTD667 has the sequence of SEQ ID NO:151, aMTD766 has the sequence of SEQ ID NO: 172 aMTD783 has the sequenceof SEQ ID NO: 174, aMTD850 has the sequence of SEQ ID NO: 203, aMTD865has the sequence of SEQ ID NO: 208, aMTD162 has the sequence of SEQ IDNO: 40, aMTD184 has the sequence of SEQ ID NO: 46, aMTD483 has thesequence of SEQ ID NO: 112, aMTD484 has the sequence of SEQ ID NO: 113,aMTD481 has the sequence of SEQ ID NO: 110, aMTD544 has the sequence ofSEQ ID NO: 127, aMTD561 has the sequence of SEQ ID NO: 129, aMTD565 hasthe sequence of SEQ ID NO: 133, aMTD583 has the sequence of SEQ ID NO:135, aMTD585 has the sequence of SEQ ID NO: 136, aMTD803 has thesequence of SEQ ID NO: 179, aMTD805 has the sequence of SEQ ID NO: 180,aMTD811 has the sequence of SEQ ID NO: 186, aMTD824 has the sequence ofSEQ ID NO: 187, aMTD825 has the sequence of SEQ ID NO: 188, aMTD826 hasthe sequence of SEQ ID NO: 189, aMTD828 has the sequence of SEQ ID NO:191, aMTD829 has the sequence of SEQ ID NO: 192, aMTD851 has thesequence of SEQ ID NO: 204, aMTD164 has the sequence of SEQ ID NO: 42,aMTD623 has the sequence of SEQ ID NO: 143, aMTD764 has the sequence ofSEQ ID NO: 170, aMTD784 has the sequence of SEQ ID NO: 175, aMTD788 hasthe sequence of SEQ ID NO: 178, aMTD808 has the sequence of SEQ ID NO:183, aMTD847 has the sequence of SEQ ID NO: 200, aMTD664 has thesequence of SEQ ID NO: 148, aMTD666 has the sequence of SEQ ID NO: 150,aMTD725 has the sequence of SEQ ID NO: 162, aMTD743 has the sequence ofSEQ ID NO: 165, aMTD744 has the sequence of SEQ ID NO: 166, aMTD747 hasthe sequence of SEQ ID NO: 168, aMTD809 has the sequence of SEQ ID NO:184, aMTD830 has the sequence of SEQ ID NO: 193, aMTD703 has thesequence of SEQ ID NO: 157, aMTD705 has the sequence of SEQ ID NO: 158,aMTD707 has the sequence of SEQ ID NO: 160, aMTD727 has the sequence ofSEQ ID NO: 164, aMTD787 has the sequence of SEQ ID NO: 177, aMTD810 hasthe sequence of SEQ ID NO: 185, aMTD827 has the sequence of SEQ ID NO:190, aMTD844 has the sequence of SEQ ID NO: 197, aMTD852 has thesequence of SEQ ID NO: 205, aMTD882 has the sequence of SEQ ID NO: 218,aMTD885 has the sequence of SEQ ID NO: 220, aMTD888 has the sequence ofSEQ ID NO: 222, aMTD889 has the sequence of SEQ ID NO: 223, aMTD891 hasthe sequence of SEQ ID NO: 224, aMTD845 has the sequence of SEQ ID NO:198, aMTD896 has the sequence of SEQ ID NO: 227, aMTD904 has thesequence of SEQ ID NO: 233, aMTD905 has the sequence of SEQ ID NO: 234,aMTD906 has the sequence of SEQ ID NO: 235, aMTD907 has the sequence ofSEQ ID NO: 236, aMTD908 has the sequence of SEQ ID NO: 237, aMTD910 hasthe sequence of SEQ ID NO: 238, aMTD911 has the sequence of SEQ ID NO:239, aMTD832 has the sequence of SEQ ID NO: 195, aMTD843 has thesequence of SEQ ID NO: 196, aMTD863 has the sequence of SEQ ID NO: 206,aMTD867 has the sequence of SEQ ID NO: 209, aMTD868 has the sequence ofSEQ ID NO: 210, aMTD883 has the sequence of SEQ ID NO: 219, aMTD901 hasthe sequence of SEQ ID NO: 231, aMTD831 has the sequence of SEQ ID NO:194, aMTD870 has the sequence of SEQ ID NO: 211, aMTD223 has thesequence of SEQ ID NO: 53, aMTD224 has the sequence of SEQ ID NO: 54,aMTD765 has the sequence of SEQ ID NO: 171, aMTD897 has the sequence ofSEQ ID NO: 228.

In FIG. 3c , aMTD302 has the sequence of SEQ ID NO: 71, aMTD361 has thesequence of SEQ ID NO: 84, aMTD502 has the sequence of SEQ ID NO: 116,aMTD545 has the sequence of SEQ ID NO: 128, aMTD605 has the sequence ofSEQ ID NO: 141, aMTD622 has the sequence of SEQ ID NO: 142, aMTD686 hasthe sequence of SEQ ID NO: 155, aMTD665 has the sequence of SEQ ID NO:149, aMTD806 has the sequence of SEQ ID NO: 181, aMTD912 has thesequence of SEQ ID NO: 240, aMTD724 has the sequence of SEQ ID NO: 161,aMTD726 has the sequence of SEQ ID NO: 163, aMTD807 has the sequence ofSEQ ID NO: 182, aMTD872 has the sequence of SEQ ID NO: 212, aMTD881 hasthe sequence of SEQ ID NO: 217, aMTD878 has the sequence of SEQ ID NO:215, aMTD879 has the sequence of SEQ ID NO: 216, aMTD241 has thesequence of SEQ ID NO: 56, aMTD221 has the sequence of SEQ ID NO: 51,aMTD222 has the sequence of SEQ ID NO: 52, aMTD242 has the sequence ofSEQ ID NO: 57, aMTD261 has the sequence of SEQ ID NO: 60, aMTD521 hasthe sequence of SEQ ID NO: 120, aMTD11 has the sequence of SEQ ID NO: 6,aMTD13 has the sequence of SEQ ID NO: 8, aMTD887 has the sequence of SEQID NO: 221, aMTD893 has the sequence of SEQ ID NO: 225, aMTD899 has thesequence of SEQ ID NO: 229, aMTD900 has the sequence of SEQ ID NO: 230,aMTD143 has the sequence of SEQ ID NO: 36, aMTD24 has the sequence ofSEQ ID NO: 12, aMTD123 has the sequence of SEQ ID NO: 33, aMTD183 hasthe sequence of SEQ ID NO: 45, aMTD262 has the sequence of SEQ ID NO:61, aMTD283 has the sequence of SEQ ID NO: 67, aMTD503 has the sequenceof SEQ ID NO: 117, aMTD465 has the sequence of SEQ ID NO: 109, aMTD687has the sequence of SEQ ID NO: 156, aMTD342 has the sequence of SEQ IDNO: 80, aMTD404 has the sequence of SEQ ID NO: 95, aMTD463 has thesequence of SEQ ID NO: 107, aMTD706 has the sequence of SEQ ID NO: 159,aMTD144 has the sequence of SEQ ID NO: 37, aMTD786 has the sequence ofSEQ ID NO: 176, aMTD895 has the sequence of SEQ ID NO: 226, aMTD541 hasthe sequence of SEQ ID NO: 124, rP6 has the sequence of SEQ ID NO: 893,rP9 has the sequence of SEQ ID NO: 912, rP19 has the sequence of SEQ IDNO: 942, rP20 has the sequence of SEQ ID NO: 946, rP37 has the sequenceof SEQ ID NO: 889, rP40 has the sequence of SEQ ID NO: 948, rP54 has thesequence of SEQ ID NO: 939, rP27 has the sequence of SEQ ID NO: 885,rP49 has the sequence of SEQ ID NO: 918, rP59 has the sequence of SEQ IDNO: 938, rP152 has the sequence of SEQ ID NO: 884, rP30 has the sequenceof SEQ ID NO: 926, rP33 has the sequence of SEQ ID NO: 927, rP68 has thesequence of SEQ ID NO: 913, rP69 has the sequence of SEQ ID NO: 908,rP131 has the sequence of SEQ ID NO: 928, rP227 has the sequence of SEQID NO: 904, rP138 has the sequence of SEQ ID NO: 933, rP935 has thesequence of SEQ ID NO: 886, rP947 has the sequence of SEQ ID NO: 943,rP949 has the sequence of SEQ ID NO: 940, rP169 has the sequence of SEQID NO: 923, rP16 has the sequence of SEQ ID NO: 890, rP17 has thesequence of SEQ ID NO: 905, rP18 has the sequence of SEQ ID NO: 945,rP28 has the sequence of SEQ ID NO: 921, rP29 has the sequence of SEQ IDNO: 924, rP246 has the sequence of SEQ ID NO: 899, rP248 has thesequence of SEQ ID NO: 903, rP66 has the sequence of SEQ ID NO: 902,rP67 has the sequence of SEQ ID NO: 906, rP113 has the sequence of SEQID NO: 891, rP159 has the sequence of SEQ ID NO: 950, rP190 has thesequence of SEQ ID NO: 925, rP349 has the sequence of SEQ ID NO: rP350has the sequence of SEQ ID NO: rP390 has the sequence of SEQ ID NO: 914,rP426 has the sequence of SEQ ID NO: 900, rP436 has the sequence of SEQID NO: 932, rP635 has the sequence of SEQ ID NO: 947, rP921 has thesequence of SEQ ID NO: 931, rP922 has the sequence of SEQ ID NO: 929.

In FIG. 3d , rP527 has the sequence of SEQ ID NO: 896, rP606 has thesequence of SEQ ID NO: 901, rP750 has the sequence of SEQ ID NO: 894,rP329 has the sequence of SEQ ID NO: 917, rP466 has the sequence of SEQID NO: 897, rP934 has the sequence of SEQ ID NO: 888, rP936 has thesequence of SEQ ID NO: 883, rP937 has the sequence of SEQ ID NO: 915,rP938 has the sequence of SEQ ID NO: 916, rP26 has the sequence of SEQID NO: 895, rP39 has the sequence of SEQ ID NO: rP97 has the sequence ofSEQ ID NO: rP139 has the sequence of SEQ ID NO: 941, rP226 has thesequence of SEQ ID NO: 892, rP210 has the sequence of SEQ ID NO: 92,rP214 has the sequence of SEQ ID NO: 937, rP692 has the sequence of SEQID NO: 907, rP693 has the sequence of SEQ ID NO: 922, rP700 has thesequence of SEQ ID NO: 951, rP57 has the sequence of SEQ ID NO: 949,rP71 has the sequence of SEQ ID NO: 930, rP77 has the sequence of SEQ IDNO: 934, rP167 has the sequence of SEQ ID NO: 898, rP331 has thesequence of SEQ ID NO: 911, rP577 has the sequence of SEQ ID NO: 935,rP772 has the sequence of SEQ ID NO: 919.

FIGS. 4a and 4b show Purification of aMTD- or rPeptide-Fused RecombinantProteins. Expressed recombinant proteins were purified by Ni²+ affinitychromatography under the natural condition. Purification of recombinantproteins displayed through SDS-PAGE analysis.

In FIG. 4a , aMTD1 has the sequence of SEQ ID NO: 1, aMTD 2 has thesequence of SEQ ID NO: 2, aMTD3 has the sequence of SEQ ID NO: 3, aMTD4has the sequence of SEQ ID NO: 4, aMTD5 has the sequence of SEQ ID NO:5, aMTD21 has the sequence of SEQ ID NO: 9, aMTD124 has the sequence ofSEQ ID NO: 34, aMTD161 has the sequence of SEQ ID NO: 39, aMTD165 hasthe sequence of SEQ ID NO: 43, aMTD182 has the sequence of SEQ ID NO:44, aMTD204 has the sequence of SEQ ID NO: 49, aMTD12 has the sequenceof SEQ ID NO: 7, aMTD24 has the sequence of SEQ ID NO: 12, aMTD25 hasthe sequence of SEQ ID NO: 13, aMTD43 has the sequence of SEQ ID NO: 15,aMTD44 has the sequence of SEQ ID NO: 16, aMTD62 has the sequence of SEQID NO: 18, aMTD63 has the sequence of SEQ ID NO: 19, aMTD23 has thesequence of SEQ ID NO: 11, aMTD61 has the sequence of SEQ ID NO: 17,aMTD82 has the sequence of SEQ ID NO: 23, aMTD284 has the sequence ofSEQ ID NO: 68, aMTD64 has the sequence of SEQ ID NO: 20, aMTD65 has thesequence of SEQ ID NO: 21, aMTD83 has the sequence of SEQ ID NO: 24,aMTD85 has the sequence of SEQ ID NO:26, aMTD102 has the sequence of SEQID NO: 28, aMTD264 has the sequence of SEQ ID NO: 63, aMTD285 has thesequence of SEQ ID NO:69, aMTD103 has the sequence of SEQ ID NO: 29,aMTD104 has the sequence of SEQ ID NO: 30, aMTD105 has the sequence ofSEQ ID NO: 31, aMTD121 has the sequence of SEQ ID NO: 32, aMTD123 hasthe sequence of SEQ ID NO: 33, aMTD141 has the sequence of SEQ ID NO:35, aMTD143 has the sequence of SEQ ID NO: 36, aMTD145 has the sequenceof SEQ ID NO: 38, aMTD42 has the sequence of SEQ ID NO: 14, aMTD163 hasthe sequence of SEQ ID NO: 41, aMTD809 has the sequence of SEQ ID NO:184, aMTD667 has the sequence of SEQ ID NO: 151, aMTD13 has the sequenceof SEQ ID NO: 8, aMTD205 has the sequence of SEQ ID NO: 50, aMTD243 hasthe sequence of SEQ ID NO: 58, aMTD245 has the sequence of SEQ ID NO:59, aMTD282 has the sequence of SEQ ID NO: 66, aMTD301 has the sequenceof SEQ ID NO: 70, aMTD304 has the sequence of SEQ ID NO: 72, aMTD305 hasthe sequence of SEQ ID NO: 73, aMTD281 has the sequence of SEQ ID NO:65, aMTD321 has the sequence of SEQ ID NO: 74, aMTD343 has the sequenceof SEQ ID NO: 81, aMTD385 has the sequence of SEQ ID NO: 91, aMTD341 hasthe sequence of SEQ ID NO: 79, aMTD522 has the sequence of SEQ ID NO:121, aMTD201 has the sequence of SEQ ID NO: 48, aMTD361 has the sequenceof SEQ ID NO: 83, aMTD183 has the sequence of SEQ ID NO: 45, aMTD222 hasthe sequence of SEQ ID NO: 52, aMTD262 has the sequence of SEQ ID NO:61, aMTD265 has the sequence of SEQ ID NO: 64, aMTD283 has the sequenceof SEQ ID NO: 67, aMTD363 has the sequence of SEQ ID NO: 84, aMTD365 hasthe sequence of SEQ ID NO: 86, aMTD364 has the sequence of SEQ ID NO:85, aMTD381 has the sequence of SEQ ID NO: 87, aMTD383 has the sequenceof SEQ ID NO: 89, aMTD384 has the sequence of SEQ ID NO: 90, aMTD402 hasthe sequence of SEQ ID NO: 93, aMTD703 has the sequence of SEQ ID NO:157, aMTD724 has the sequence of SEQ ID NO: 161, aMTD726 has thesequence of SEQ ID NO:163, aMTD845 has the sequence of SEQ ID NO: 198,aMTD897 has the sequence of SEQ ID NO: 228, aMTD831 has the sequence ofSEQ ID NO: 194, aMTD881 has the sequence of SEQ ID NO: 217, aMTD544 hasthe sequence of SEQ ID NO: 127, aMTD561 has the sequence of SEQ ID NO:129, aMTD583 has the sequence of SEQ ID NO: 135, aMTD585 has thesequence of SEQ ID NO: 136, aMTD763 has the sequence of SEQ ID NO: 169,aMTD846 has the sequence of SEQ ID NO: 199, aMTD849 has the sequence ofSEQ ID NO: 202, aMTD864 has the sequence of SEQ ID NO: 207, aMTD11 hasthe sequence of SEQ ID NO: 6, aMTD261 has the sequence of SEQ ID NO: 60,aMTD521 has the sequence of SEQ ID NO: 120, aMTD524 has the sequence ofSEQ ID NO: 122, aMTD542 has the sequence of SEQ ID NO: 125, aMTD545 hasthe sequence of SEQ ID NO: 128, aMTD564 has the sequence of SEQ ID NO:132, aMTD582 has the sequence of SEQ ID NO: 134, aMTD601 has thesequence of SEQ ID NO: 137, aMTD603 has the sequence of SEQ ID NO: 139,aMTD625 has the sequence of SEQ ID NO: 144, aMTD322 has the sequence ofSEQ ID NO: 75, aMTD323 has the sequence of SEQ ID NO: 76, aMTD403 hasthe sequence of SEQ ID NO: 94, aMTD404 has the sequence of SEQ ID NO:95, aMTD405 has the sequence of SEQ ID NO: 96, aMTD421 has the sequenceof SEQ ID NO: 97, aMTD422 has the sequence of SEQ ID NO: 98, aMTD424 hasthe sequence of SEQ ID NO: 99, aMTD425 has the sequence of SEQ ID NO:100, aMTD442 has the sequence of SEQ ID NO: 101, aMTD443 has thesequence of SEQ ID NO: 102, aMTD445 has the sequence of SEQ ID NO: 104,aMTD461 has the sequence of SEQ ID NO: 105, aMTD525 has the sequence ofSEQ ID NO: 123, aMTD543 has the sequence of SEQ ID NO: 126, aMTD645 hasthe sequence of SEQ ID NO: 146, aMTD462 has the sequence of SEQ ID NO:106, aMTD463 has the sequence of SEQ ID NO: 107, aMTD81 has the sequenceof SEQ ID NO: 22, aMTD221 has the sequence of SEQ ID NO: 51, aMTD302 hasthe sequence of SEQ ID NO: 71, aMTD562 has the sequence of SEQ ID NO:130, aMTD563 has the sequence of SEQ ID NO: 131, aMTD241 has thesequence of SEQ ID NO: 56, aMTD830 has the sequence of SEQ ID NO: 193,aMTD832 has the sequence of SEQ ID NO: 195, aMTD883 has the sequence ofSEQ ID NO: 219, aMTD843 has the sequence of SEQ ID NO: 196, aMTD863 hasthe sequence of SEQ ID NO: 206, aMTD868 has the sequence of SEQ ID NO:210, aMTD867 has the sequence of SEQ ID NO: 209, aMTD901 has thesequence of SEQ ID NO: 231, aMTD482 has the sequence of SEQ ID NO: 111,aMTD505 has the sequence of SEQ ID NO: 119, aMTD664 has the sequence ofSEQ ID NO: 148, aMTD686 has the sequence of SEQ ID NO: 155, aMTD382 hasthe sequence of SEQ ID NO: 88, aMTD325 has the sequence of SEQ ID NO:78, aMTD324 has the sequence of SEQ ID NO: 77, aMTD263 has the sequenceof SEQ ID NO: 62, aMTD225 has the sequence of SEQ ID NO: 55, aMTD661 hasthe sequence of SEQ ID NO: 147, aMTD686 has the sequence of SEQ ID NO:155, aMTD602 has the sequence of SEQ ID NO: 138, aMTD485 has thesequence of SEQ ID NO: 114, aMTD483 has the sequence of SEQ ID NO: 112,aMTD22 has the sequence of SEQ ID NO: 10, aMTD42 has the sequence of SEQID NO: 14, aMTD84 has the sequence of SEQ ID NO: 25, aMTD401 has thesequence of SEQ ID NO: 92, aMTD444 has the sequence of SEQ ID NO: 103,aMTD464 has the sequence of SEQ ID NO: 108, aMTD501 has the sequence ofSEQ ID NO: 115, aMTD503 has the sequence of SEQ ID NO: 117, aMTD605 hasthe sequence of SEQ ID NO: 141, aMTD622 has the sequence of SEQ ID NO:142, aMTD144 has the sequence of SEQ ID NO: 37, aMTD484 has the sequenceof SEQ ID NO: 113, aMTD665 has the sequence of SEQ ID NO: 149, aMTD806has the sequence of SEQ ID NO: 181, aMTD875 has the sequence of SEQ IDNO: 213, aMTD877 has the sequence of SEQ ID NO: 214, aMTD887 has thesequence of SEQ ID NO: 221, aMTD893 has the sequence of SEQ ID NO: 225,aMTD912 has the sequence of SEQ ID NO: 240, aMTD185 has the sequence ofSEQ ID NO: 47, aMTD164 has the sequence of SEQ ID NO: 42, aMTD184 hasthe sequence of SEQ ID NO: 46, aMTD899 has the sequence of SEQ ID NO:229, aMTD904 has the sequence of SEQ ID NO: 233, aMTD101 has thesequence of SEQ ID NO: 27, aMTD223 has the sequence of SEQ ID NO: 53,aMTD224 has the sequence of SEQ ID NO: 54, aMTD345 has the sequence ofSEQ ID NO: 82, aMTD481 has the sequence of SEQ ID NO: 110, aMTD900 hasthe sequence of SEQ ID NO: 230, aMTD905 has the sequence of SEQ ID NO:234, aMTD906 has the sequence of SEQ ID NO: 235, aMTD908 has thesequence of SEQ ID NO: 237, aMTD910 has the sequence of SEQ ID NO: 238,aMTD911 has the sequence of SEQ ID NO: 239, aMTD828 has the sequence ofSEQ ID NO: 191, aMTD829 has the sequence of SEQ ID NO: 192, aMTD852 hasthe sequence of SEQ ID NO: 205, aMTD882 has the sequence of SEQ ID NO:218, aMTD885 has the sequence of SEQ ID NO: 220, aMTD888 has thesequence of SEQ ID NO: 222, aMTD889 has the sequence of SEQ ID NO: 223,aMTD891 has the sequence of SEQ ID NO: 224, aMTD727 has the sequence ofSEQ ID NO: 164, aMTD786 has the sequence of SEQ ID NO: 176, aMTD787 hasthe sequence of SEQ ID NO:177, aMTD803 has the sequence of SEQ ID NO:179, aMTD805 has the sequence of SEQ ID NO: 180, aMTD810 has thesequence of SEQ ID NO: 185, aMTD811 has the sequence of SEQ ID NO:186,aMTD824 has the sequence of SEQ ID NO: 187, aMTD604 has the sequence ofSEQ ID NO: 140, aMTD684 has the sequence of SEQ ID NO: 153, aMTD685 hasthe sequence of SEQ ID NO: 154, aMTD683 has the sequence of SEQ ID NO:152, aMTD687 has the sequence of SEQ ID NO: 156, aMTD765 has thesequence of SEQ ID NO: 171, aMTD767 has the sequence of SEQ ID NO: 173,aMTD706 has the sequence of SEQ ID NO: 159, aMTD865 has the sequence ofSEQ ID NO: 208, aMTD465 has the sequence of SEQ ID NO: 109, aMTD162 hasthe sequence of SEQ ID NO: 40, aMTD705 has the sequence of SEQ ID NO:158, aMTD707 has the sequence of SEQ ID NO: 160, aMTD725 has thesequence of SEQ ID NO: 162, aMTD743 has the sequence of SEQ ID NO: 165,aMTD744 has the sequence of SEQ ID NO: 166.

In FIG. 4b , aMTD623 has the sequence of SEQ ID NO: 143, aMTD764 has thesequence of SEQ ID NO: 170, aMTD783 has the sequence of SEQ ID NO: 174,aMTD784 has the sequence of SEQ ID NO: 175, aMTD788 has the sequence ofSEQ ID NO:178, aMTD808 has the sequence of SEQ ID NO: 183, aMTD848 hasthe sequence of SEQ ID NO: 201, aMTD850 has the sequence of SEQ ID NO:203, aMTD826 has the sequence of SEQ ID NO: 189, aMTD827 has thesequence of SEQ ID NO: 190, aMTD502 has the sequence of SEQ ID NO: 116,aMTD643 has the sequence of SEQ ID NO: 145, aMTD807 has the sequence ofSEQ ID NO: 182, aMTD844 has the sequence of SEQ ID NO: 197, aMTD851 hasthe sequence of SEQ ID NO: 204, aMTD870 has the sequence of SEQ ID NO:211, aMTD872 has the sequence of SEQ ID NO: 212, aMTD878 has thesequence of SEQ ID NO: 215, aMTD879 has the sequence of SEQ ID NO: 216,aMTD902 has the sequence of SEQ ID NO: 232, aMTD907 has the sequence ofSEQ ID NO: 236, aMTD504 has the sequence of SEQ ID NO: 118, aMTD565 hasthe sequence of SEQ ID NO: 133, aMTD746 has the sequence of SEQ ID NO:167, aMTD766 has the sequence of SEQ ID NO: 172, aMTD825 has thesequence of SEQ ID NO: 188, aMTD847 has the sequence of SEQ ID NO: 200,aMTD895 has the sequence of SEQ ID NO: 226, aMTD896 has the sequence ofSEQ ID NO: 227, aMTD541 has the sequence of SEQ ID NO: 124, aMTD342 hasthe sequence of SEQ ID NO: 80, aMTD747 has the sequence of SEQ ID NO:168, rP19 has the sequence of SEQ ID NO: 942, rP20 has the sequence ofSEQ ID NO: 946, rP27 has the sequence of SEQ ID NO: 885, rP29 has thesequence of SEQ ID NO: 924, rP37 has the sequence of SEQ ID NO: 889,rP152 has the sequence of SEQ ID NO: 884, rP169 has the sequence of SEQID NO: 923, rP69 has the sequence of SEQ ID NO: 908, rP68 has thesequence of SEQ ID NO: 913, rP40 has the sequence of SEQ ID NO: 948,rP49 has the sequence of SEQ ID NO: 918, rP54 has the sequence of SEQ IDNO: 939, rP226 has the sequence of SEQ ID NO: 892, rP6 has the sequenceof SEQ ID NO: 893, rP131 has the sequence of SEQ ID NO: 928, rP30 hasthe sequence of SEQ ID NO: 926, rP167 has the sequence of SEQ ID NO:898, rP329 has the sequence of SEQ ID NO: 917, rP466 has the sequence ofSEQ ID NO: 897, rP527 has the sequence of SEQ ID NO: 896, rP606 has thesequence of SEQ ID NO: 901, rP750 has the sequence of SEQ ID NO: 894,rP246 has the sequence of SEQ ID NO: 899, rP248 has the sequence of SEQID NO: 903, rP59 has the sequence of SEQ ID NO: 938, rP390 has thesequence of SEQ ID NO: 909, rP426 has the sequence of SEQ ID NO: 900,rP66 has the sequence of SEQ ID NO: 902, rP227 has the sequence of SEQID NO: 904, rP349 has the sequence of SEQ ID NO: 914, rP33 has thesequence of SEQ ID NO: 927, rP436 has the sequence of SEQ ID NO: 932,rP77 has the sequence of SEQ ID NO: 934, rP935 has the sequence of SEQID NO: 886, rP57 has the sequence of SEQ ID NO: 949, rP577 has thesequence of SEQ ID NO: 935, rP921 has the sequence of SEQ ID NO: 931,rP922 has the sequence of SEQ ID NO: 929, rP26 has the sequence of SEQID NO: 895, rP39 has the sequence of SEQ ID NO: 941, rP97 has thesequence of SEQ ID NO: 936, rP139 has the sequence of SEQ ID NO: 944,rP67 has the sequence of SEQ ID NO: 906, rP113 has the sequence of SEQID NO: 891, rP190 has the sequence of SEQ ID NO: 925, rP210 has thesequence of SEQ ID NO: 920, rP214 has the sequence of SEQ ID NO: 937,rP635 has the sequence of SEQ ID NO: 947, rP692 has the sequence of SEQID NO: 907, rP693 has the sequence of SEQ ID NO: 922, rP772 has thesequence of SEQ ID NO: 919, rP934 has the sequence of SEQ ID NO: 888,rP936 has the sequence of SEQ ID NO: 883, rP937 has the sequence of SEQID NO: 915, rP938 has the sequence of SEQ ID NO: 916, rP350 has thesequence of SEQ ID NO: 910, rP331 has the sequence of SEQ ID NO: 911,rP947 has the sequence of SEQ ID NO: 943, rP16 has the sequence of SEQID NO: 890, rP17 has the sequence of SEQ ID NO: 905, rP18 has thesequence of SEQ ID NO: 945, rP28 has the sequence of SEQ ID NO: 921,rP159 has the sequence of SEQ ID NO: 950, rP700 has the sequence of SEQID NO: 951, rP71 has the sequence of SEQ ID NO: 930.

FIGS. 5a to 5u show Determination of aMTD-Mediated Cell-Permeability.Cell-permeability of a negative control (A: rP38 (SEQ ID NO: 952)) andreference hydrophobic CPPs (MTM12 (SEQ ID NO: 953) and MTD85 (SEQ ID NO:954)) are shown. The cell-permeability of each aMTD and/or rPeptide isvisually compared to that of the cargo protein lacking peptide sequence(HCA). Gray shaded area represents untreated RAW 264.7 cells (vehicle);thin light gray line represents the cells treated with equal molarconcentration of FITC (FITC only); dark thick line indicates the cellstreated with FITC-his-tagged CRA protein (HCA); and the cells treatedwith the FITC-proteins (HMCA) fused to negative control (rP38 (SEQ IDNO: 952)), reference CPP (MTM12 (SEQ ID NO: 953) or MTD85 (SEQ ID NO:954)) or new hydrophobic CPP (aMTD) are shown with light thick line andindicated by arrows.

In FIG. 5a , rP38 has the sequence of SEQ ID NO: 952, MTM12 has thesequence of SEQ ID NO: 953, MTD85 has the sequence of SEQ ID NO: 954,aMTD285 has the sequence of SEQ ID NO: 69, aMTD361 has the sequence ofSEQ ID NO: 83, aMTD301 has the sequence of SEQ ID NO: 70, aMTD305 hasthe sequence of SEQ ID NO: 73, aMTD343 has the sequence of SEQ ID NO:81, and aMTD243 has the sequence of SEQ ID NO: 58. In FIG. 5b , aMTD282has the sequence of SEQ ID NO: 66, aMTD245 has the sequence of SEQ IDNO: 59, aMTD302 has the sequence of SEQ ID NO: 71, aMTD264 has thesequence of SEQ ID NO: 63, aMTD341 has the sequence of SEQ ID NO: 79,aMTD645 has the sequence of SEQ ID NO: 146, aMTD201 has the sequence ofSEQ ID NO: 48, aMTD13 has the sequence of SEQ ID NO: 8, aMTD522 has thesequence of SEQ ID NO: 121, aMTD24 has the sequence of SEQ ID NO: 59,aMTD283 has the sequence of SEQ ID NO: 67, and aMTD262 has the sequenceof SEQ ID NO: 301, In FIG. 5c , aMTD304 has the sequence of SEQ ID NO:72, aMTD265 has the sequence of SEQ ID NO: 64, aMTD123 has the sequenceof SEQ ID NO: 33, aMTD183 has the sequence of SEQ ID NO: 45, aMTD143 hasthe sequence of SEQ ID NO: 36, aMTD900 has the sequence of SEQ ID NO:230, aMTD807 has the sequence of SEQ ID NO: 182, aMTD381 has thesequence of SEQ ID NO: 87, aMTD601 has the sequence of SEQ ID NO: 137,aMTD603 has the sequence of SEQ ID NO: 139, aMTD383 has the sequence ofSEQ ID NO: 89, and aMTD261 has the sequence of SEQ ID NO: 60, In FIG. 5d, aMTD807 has the sequence of SEQ ID NO: 182, aMTD381 has the sequenceof SEQ ID NO: 87, aMTD601 has the sequence of SEQ ID NO: 137, aMTD603has the sequence of SEQ ID NO: 139, aMTD383 has the sequence of SEQ IDNO: 89, aMTD261 has the sequence of SEQ ID NO: 60, aMTD364 has thesequence of SEQ ID NO: 85, aMTD564 has the sequence of SEQ ID NO: 132,aMTD384 has the sequence of SEQ ID NO: 90, aMTD625 has the sequence ofSEQ ID NO: 144, aMTD543 has the sequence of SEQ ID NO: 126, and aMTD545has the sequence of SEQ ID NO: 128.

In FIG. 5e , aMTD363 has the sequence of SEQ ID NO: 84, aMTD342 has thesequence of SEQ ID NO: 80, aMTD382 has the sequence of SEQ ID NO: 88,aMTD443 has the sequence of SEQ ID NO: 102, aMTD829 has the sequence ofSEQ ID NO: 192, aMTD421 has the sequence of SEQ ID NO: 97, aMTD241 hasthe sequence of SEQ ID NO: 56, aMTD284 has the sequence of SEQ ID NO:68, aMTD525 has the sequence of SEQ ID NO: 123, aMTD442 has the sequenceof SEQ ID NO: 101, aMTD422 has the sequence of SEQ ID NO: 98, aMTD424has the sequence of SEQ ID NO: 99.

In FIG. 5f , aMTD405 has the sequence of SEQ ID NO: 96, aMTD323 has thesequence of SEQ ID NO: 76, aMTD84 has the sequence of SEQ ID NO: 25,aMTD62 has the sequence of SEQ ID NO: 18, aMTD63 has the sequence of SEQID NO: 19, aMTD25 has the sequence of SEQ ID NO: 13, aMTD889 has thesequence of SEQ ID NO: 223, aMTD504 has the sequence of SEQ ID NO: 118,aMTD103 has the sequence of SEQ ID NO: 29, aMTD23 has the sequence ofSEQ ID NO: 11, aMTD82 has the sequence of SEQ ID NO: 23, aMTD105 has thesequence of SEQ ID NO: 31. In FIG. 5g , aMTD462 has the sequence of SEQID NO: 106, aMTD461 has the sequence of SEQ ID NO: 105, aMTD1 has thesequence of SEQ ID NO: 1, aMTD2 has the sequence of SEQ ID NO: 2, aMTD61has the sequence of SEQ ID NO: 17, aMTD124 has the sequence of SEQ IDNO: 34, aMTD165 has the sequence of SEQ ID NO: 43, aMTD404 has thesequence of SEQ ID NO: 95, aMTD182 has the sequence of SEQ ID NO: 44,aMTD3 has the sequence of SEQ ID NO: 3, aMTD204 has the sequence of SEQID NO: 49, aMTD141 has the sequence of SEQ ID NO: 35. In FIG. 5h ,aMTD524 has the sequence of SEQ ID NO: 122, aMTD22 has the sequence ofSEQ ID NO: 10, aMTD65 has the sequence of SEQ ID NO: 21, aMTD83 has thesequence of SEQ ID NO: 24, aMTD4 has the sequence of SEQ ID NO:4, aMTD5has the sequence of SEQ ID NO: 5, aMTD81 has the sequence of SEQ ID NO:22, aMTD44 has the sequence of SEQ ID NO: 16, aMTD145 has the sequenceof SEQ ID NO: 38, aMTD43 has the sequence of SEQ ID NO: 15, aMTD104 hasthe sequence of SEQ ID NO: 30, aMTD221 has the sequence of SEQ ID NO:51. In FIG. 5i , aMTD222 has the sequence of SEQ ID NO: 52, aMTD242 hasthe sequence of SEQ ID NO: 57, aMTD827 has the sequence of SEQ ID NO:190, aMTD501 has the sequence of SEQ ID NO: 115, aMTD726 has thesequence of SEQ ID NO: 163, aMTD706 has the sequence of SEQ ID NO: 159,aMTD602 has the sequence of SEQ ID NO: 138, aMTD281 has the sequence ofSEQ ID NO: 65, aMTD683 has the sequence of SEQ ID NO: 152, aMTD893 hasthe sequence of SEQ ID NO: 225, aMTD879 has the sequence of SEQ ID NO:216, aMTD746 has the sequence of SEQ ID NO: 167. In FIG. 5j , aMTD864has the sequence of SEQ ID NO: 207, aMTD865 has the sequence of SEQ IDNO: 208, aMTD604 has the sequence of SEQ ID NO: 140, aMTD765 has thesequence of SEQ ID NO: 171, aMTD163 has the sequence of SEQ ID NO: 41,aMTD684 has the sequence of SEQ ID NO: 153, aMTD783 has the sequence ofSEQ ID NO: 174, aMTD846 has the sequence of SEQ ID NO: 199, aMTD483 hasthe sequence of SEQ ID NO: 112, aMTD685 has the sequence of SEQ ID NO:154, aMTD585 has the sequence of SEQ ID NO: 136, aMTD687 has thesequence of SEQ ID NO: 156. In FIG. 5k , aMTD541 has the sequence of SEQID NO: 124, aMTD485 has the sequence of SEQ ID NO: 114, aMTD784 has thesequence of SEQ ID NO: 175, aMTD184 has the sequence of SEQ ID NO: 46,aMTD764 has the sequence of SEQ ID NO: 170, aMTD808 has the sequence ofSEQ ID NO: 183, aMTD544 has the sequence of SEQ ID NO: 127, aMTD224 hasthe sequence of SEQ ID NO: 54, aMTD848 has the sequence of SEQ ID NO:201, aMTD766 has the sequence of SEQ ID NO: 172, aMTD164 has thesequence of SEQ ID NO: 42, aMTD565 has the sequence of SEQ ID NO: 133.In FIG. 51, aMTD847 has the sequence of SEQ ID NO: 200, aMTD850 has thesequence of SEQ ID NO: 203, aMTD225 has the sequence of SEQ ID NO: 55,aMTD686 has the sequence of SEQ ID NO: 155, aMTD64 has the sequence ofSEQ ID NO: 20, aMTD325 has the sequence of SEQ ID NO: 78, aMTD263 hasthe sequence of SEQ ID NO: 62, aMTD667 has the sequence of SEQ ID NO:151, aMTD324 has the sequence of SEQ ID NO: 77, aMTD464 has the sequenceof SEQ ID NO: 108, aMTD482 has the sequence of SEQ ID NO: 111, aMTD425has the sequence of SEQ ID NO: 100.

In FIG. 5m , aMTD503 has the sequence of SEQ ID NO: 117, aMTD505 has thesequence of SEQ ID NO: 119, aMTD883 has the sequence of SEQ ID NO: 219,aMTD830 has the sequence of SEQ ID NO: 193, aMTD867 has the sequence ofSEQ ID NO: 209, aMTD809 has the sequence of SEQ ID NO: 184, aMTD843 hasthe sequence of SEQ ID NO: 196, aMTD622 has the sequence of SEQ ID NO:142, aMTD162 has the sequence of SEQ ID NO: 40, aMTD747 has the sequenceof SEQ ID NO: 168, aMTD743 has the sequence of SEQ ID NO: 165, aMTD705has the sequence of SEQ ID NO: 158. In FIG. 5n , aMTD725 has thesequence of SEQ ID NO: 162, aMTD832 has the sequence of SEQ ID NO: 195,aMTD852 has the sequence of SEQ ID NO: 205, aMTD810 has the sequence ofSEQ ID NO: 185, aMTD888 has the sequence of SEQ ID NO: 222, aMTD908 hasthe sequence of SEQ ID NO: 237, aMTD885 has the sequence of SEQ ID NO:220, aMTD868 has the sequence of SEQ ID NO: 210, aMTD786 has thesequence of SEQ ID NO: 176, aMTD803 has the sequence of SEQ ID NO: 179,aMTD787 has the sequence of SEQ ID NO: 177, aMTD904 has the sequence ofSEQ ID NO: 233. In FIG. 5o , aMTD882 has the sequence of SEQ ID NO: 218,aMTD910 has the sequence of SEQ ID NO: 238, aMTD899 has the sequence ofSEQ ID NO: 229, aMTD891 has the sequence of SEQ ID NO: 224, aMTD727 hasthe sequence of SEQ ID NO: 164, aMTD805 has the sequence of SEQ ID NO:180, aMTD896 has the sequence of SEQ ID NO: 227, aMTD828 has thesequence of SEQ ID NO: 191, aMTD811 has the sequence of SEQ ID NO: 186,aMTD805 has the sequence of SEQ ID NO: 180, aMTD806 has the sequence ofSEQ ID NO: 181, aMTD905 has the sequence of SEQ ID NO: 234. In FIG. 5p ,aMTD851 has the sequence of SEQ ID NO: 204, aMTD881 has the sequence ofSEQ ID NO: 217, aMTD875 has the sequence of SEQ ID NO: 213, aMTD703 hasthe sequence of SEQ ID NO: 157, aMTD887 has the sequence of SEQ ID NO:221, aMTD831 has the sequence of SEQ ID NO: 194, aMTD11 has the sequenceof SEQ ID NO: 6, aMTD912 has the sequence of SEQ ID NO: 240, aMTD897 hasthe sequence of SEQ ID NO: 228, aMTD877 has the sequence of SEQ ID NO:214, aMTD724 has the sequence of SEQ ID NO: 161, aMTD845 has thesequence of SEQ ID NO: 198. In FIG. 5q , aMTD666 has the sequence of SEQID NO: 150, aMTD605 has the sequence of SEQ ID NO: 141, aMTD401 has thesequence of SEQ ID NO: 92, aMTD444 has the sequence of SEQ ID NO: 103,aMTD826 has the sequence of SEQ ID NO: 189, aMTD907 has the sequence ofSEQ ID NO: 236, aMTD902 has the sequence of SEQ ID NO: 232, aMTD664 hasthe sequence of SEQ ID NO: 148, aMTD870 has the sequence of SEQ ID NO:211, aMTD345 has the sequence of SEQ ID NO: 82, aMTD502 has the sequenceof SEQ ID NO: 116, aMTD878 has the sequence of SEQ ID NO: 215. In FIG.5r , aMTD643 has the sequence of SEQ ID NO: 145, aMTD365 has thesequence of SEQ ID NO: 86, aMTD521 has the sequence of SEQ ID NO: 120,aMTD385 has the sequence of SEQ ID NO: 91, aMTD542 has the sequence ofSEQ ID NO: 125, aMTD562 has the sequence of SEQ ID NO: 130, aMTD563 hasthe sequence of SEQ ID NO: 131, aMTD465 has the sequence of SEQ ID NO:109, aMTD402 has the sequence of SEQ ID NO: 93, aMTD445 has the sequenceof SEQ ID NO: 104, aMTD582 has the sequence of SEQ ID NO: 134, aMTD463has the sequence of SEQ ID NO: 107. In FIG. 5s , aMTD322 has thesequence of SEQ ID NO: 75, aMTD102 has the sequence of SEQ ID NO: 28,aMTD21 has the sequence of SEQ ID NO: 9, aMTD12 has the sequence of SEQID NO: 7, aMTD101 has the sequence of SEQ ID NO: 27, aMTD665 has thesequence of SEQ ID NO: 149, aMTD403 has the sequence of SEQ ID NO: 94,aMTD121 has the sequence of SEQ ID NO: 32, aMTD42 has the sequence ofSEQ ID NO: 14, aMTD85 has the sequence of SEQ ID NO: 26, aMTD205 has thesequence of SEQ ID NO: 50, aMTD844 has the sequence of SEQ ID NO: 197.In FIG. 5t , aMTD321 has the sequence of SEQ ID NO: 74, aMTD849 has thesequence of SEQ ID NO: 202, aMTD661 has the sequence of SEQ ID NO: 147,aMTD872 has the sequence of SEQ ID NO: 212, aMTD767 has the sequence ofSEQ ID NO: 173, aMTD763 has the sequence of SEQ ID NO: 169, aMTD583 hasthe sequence of SEQ ID NO: 135, aMTD623 has the sequence of SEQ ID NO:143, aMTD144 has the sequence of SEQ ID NO: 37, aMTD101 has the sequenceof SEQ ID NO: 27, aMTD788 has the sequence of SEQ ID NO: 178, aMTD561has the sequence of SEQ ID NO: 129. In FIG. 5u , aMTD185 has thesequence of SEQ ID NO: 47, aMTD901 has the sequence of SEQ ID NO: 231,aMTD223 has the sequence of SEQ ID NO: 53, aMTD707 has the sequence ofSEQ ID NO: 160, aMTD484 has the sequence of SEQ ID NO: 113, aMTD863 hasthe sequence of SEQ ID NO: 206, aMTD911 has the sequence of SEQ ID NO:239, aMTD824 has the sequence of SEQ ID NO: 187, aMTD481 has thesequence of SEQ ID NO: 110, aMTD825 has the sequence of SEQ ID NO: 188,aMTD744 has the sequence of SEQ ID NO: 166, aMTD906 has the sequence ofSEQ ID NO: 235. FIGS. 6a to 6c show Determination of rPeptide-MediatedCell-Permeability. The cell-permeability of each aMTD and/or rPeptidewas visually compared to that of the cargo protein lacking peptidesequence (HCA). Gray shaded area represents untreated RAW 264.7 cells(vehicle); thin light gray line represents the cells treated with equalmolar concentration of FITC (FITC only); dark thick line indicates thecells treated with FITC-his-tagged CRA protein (HCA); and the cellstreated with the FITC-proteins fused to rPeptides are shown with lightthick line and indicated by arrows. In FIG. 6a , rP26 has the sequenceof SEQ ID NO: 895, rP39 has the sequence of SEQ ID NO: 941, rP426 hasthe sequence of SEQ ID NO: 900, rP139 has the sequence of SEQ ID NO:944, rP606 has the sequence of SEQ ID NO: 901, rP635 has the sequence ofSEQ ID NO: 947.

In FIG. 6b , rP97 has the sequence of SEQ ID NO: 936, rP66 has thesequence of SEQ ID NO: 902, rP921 has the sequence of SEQ ID NO: 931,rP772 has the sequence of SEQ ID NO: 919, rP693 has the sequence of SEQID NO: 922, rP113 has the sequence of SEQ ID NO: 891, rP167 has thesequence of SEQ ID NO: 898, rP700 has the sequence of SEQ ID NO: 951,rP49 has the sequence of SEQ ID NO: 918, rP390 has the sequence of SEQID NO: 909, rP934 has the sequence of SEQ ID NO: 888, rP692 has thesequence of SEQ ID NO: 907.

In FIG. 6c , rP57 has the sequence of SEQ ID NO: 949, rP67 has thesequence of SEQ ID NO: 906, rP329 has the sequence of SEQ ID NO: 917,rP17 has the sequence of SEQ ID NO: 905, rP68 has the sequence of SEQ IDNO: 913, rP938 has the sequence of SEQ ID NO: 916, rP16 has the sequenceof SEQ ID NO: 890, rP18 has the sequence of SEQ ID NO: 945, rP29 has thesequence of SEQ ID NO: 924, rP466 has the sequence of SEQ ID NO: 897,rP227 has the sequence of SEQ ID NO: 904, rP214 has the sequence of SEQID NO: 937.

FIGS. 7a to 7k show Visualized Cell-Permeability of aMTD-FusedRecombinant Proteins. NIH3T3 cells were treated with FITC-labeledprotein (10 μM) fused to aMTD for 1 hour at 37. Cell-permeability of theproteins was visualized by laser scanning confocal microscopy (LSM700version).

In FIG. 7a , rP38 has the sequence of SEQ ID NO: 952, MTM12 has thesequence of SEQ ID NO: 953, MTD85 has the sequence of SEQ ID NO: 954,aMTD1 has the sequence of SEQ ID NO: 1, aMTD2 has the sequence of SEQ IDNO: 2, aMTD3 has the sequence of SEQ ID NO: 3, aMTD4 has the sequence ofSEQ ID NO:4, aMTD5 has the sequence of SEQ ID NO: 5, aMTD11 has thesequence of SEQ ID NO: 6, aMTD13 has the sequence of SEQ ID NO: 8,aMTD21 has the sequence of SEQ ID NO: 9, aMTD22 has the sequence of SEQID NO: 10, aMTD23 has the sequence of SEQ ID NO: 11, aMTD24 has thesequence of SEQ ID NO: 12, aMTD25 has the sequence of SEQ ID NO: 13,aMTD43 has the sequence of SEQ ID NO: 15, aMTD44 has the sequence of SEQID NO: 16, aMTD61 has the sequence of SEQ ID NO: 17, aMTD63 has thesequence of SEQ ID NO: 19, aMTD64 has the sequence of SEQ ID NO: 20,aMTD65 has the sequence of SEQ ID NO: 21.

In FIG. 7b , aMTD82 has the sequence of SEQ ID NO: 23, aMTD83 has thesequence of SEQ ID NO: 24, aMTD84 has the sequence of SEQ ID NO: 25,aMTD85 has the sequence of SEQ ID NO: 26, aMTD101 has the sequence ofSEQ ID NO: 27, aMTD102 has the sequence of SEQ ID NO: 28, aMTD105 hasthe sequence of SEQ ID NO: 31, aMTD872 has the sequence of SEQ ID NO:212, aMTD121 has the sequence of SEQ ID NO: 32, aMTD123 has the sequenceof SEQ ID NO: 33, aMTD124 has the sequence of SEQ ID NO: 34, aMTD141 hasthe sequence of SEQ ID NO: 35, aMTD143 has the sequence of SEQ ID NO:36, aMTD144 has the sequence of SEQ ID NO: 37, aMTD145 has the sequenceof SEQ ID NO: 38, aMTD906 has the sequence of SEQ ID NO: 235, aMTD161has the sequence of SEQ ID NO: 39, aMTD164 has the sequence of SEQ IDNO: 42, aMTD182 has the sequence of SEQ ID NO: 44, aMTD183 has thesequence of SEQ ID NO: 45, aMTD184 has the sequence of SEQ ID NO: 46,aMTD185 has the sequence of SEQ ID NO: 47, aMTD 201 has the sequence ofSEQ ID NO: 48, aMTD204 has the sequence of SEQ ID NO: 49.

In FIG. 7c , aMTD221 has the sequence of SEQ ID NO: 51, aMTD222 has thesequence of SEQ ID NO: 52, aMTD223 has the sequence of SEQ ID NO: 53,aMTD224 has the sequence of SEQ ID NO: 54, aMTD882 has the sequence ofSEQ ID NO: 218, aMTD908 has the sequence of SEQ ID NO: 237, aMTD242 hasthe sequence of SEQ ID NO: 57, aMTD243 has the sequence of SEQ ID NO:58, aMTD245 has the sequence of SEQ ID NO: 59, aMTD261 has the sequenceof SEQ ID NO: 60, aMTD262 has the sequence of SEQ ID NO: 61, aMTD263 hasthe sequence of SEQ ID NO: 62, aMTD265 has the sequence of SEQ ID NO:64, aMTD281 has the sequence of SEQ ID NO: 65, aMTD282 has the sequenceof SEQ ID NO: 66, aMTD302 has the sequence of SEQ ID NO: 71, aMTD284 hasthe sequence of SEQ ID NO: 68, aMTD285 has the sequence of SEQ ID NO:69, aMTD881 has the sequence of SEQ ID NO: 217, aMTD325 has the sequenceof SEQ ID NO: 78, aMTD304 has the sequence of SEQ ID NO: 72, aMTD305 hasthe sequence of SEQ ID NO: 73, aMTD321 has the sequence of SEQ ID NO:74, aMTD322 has the sequence of SEQ ID NO: 75.

In FIG. 7d , aMTD879 has the sequence of SEQ ID NO: 216, aMTD893 has thesequence of SEQ ID NO: 225, aMTD341 has the sequence of SEQ ID NO: 79,aMTD342 has the sequence of SEQ ID NO: 80, aMTD343 has the sequence ofSEQ ID NO: 81, aMTD345 has the sequence of SEQ ID NO: 82, aMTD363 hasthe sequence of SEQ ID NO: 84, aMTD364 has the sequence of SEQ ID NO:85, aMTD365 has the sequence of SEQ ID NO: 86, aMTD381 has the sequenceof SEQ ID NO: 87, aMTD382 has the sequence of SEQ ID NO: 88, aMTD383 hasthe sequence of SEQ ID NO: 89, aMTD385 has the sequence of SEQ ID NO:91, aMTD401 has the sequence of SEQ ID NO: 92, aMTD402 has the sequenceof SEQ ID NO: 93, aMTD403 has the sequence of SEQ ID NO: 94, aMTD404 hasthe sequence of SEQ ID NO: 95, aMTD405 has the sequence of SEQ ID NO:96, aMTD422 has the sequence of SEQ ID NO: 98, aMTD424 has the sequenceof SEQ ID NO: 99, aMTD425 has the sequence of SEQ ID NO: 100, aMTD910has the sequence of SEQ ID NO: 238, aMTD442 has the sequence of SEQ IDNO: 101, aMTD443 has the sequence of SEQ ID NO: 102.

In FIG. 7e , aMTD461 has the sequence of SEQ ID NO: 105, aMTD462 has thesequence of SEQ ID NO: 106, aMTD463 has the sequence of SEQ ID NO: 107,aMTD464 has the sequence of SEQ ID NO: 108, aMTD465 has the sequence ofSEQ ID NO: 109, aMTD481 has the sequence of SEQ ID NO: 110, aMTD483 hasthe sequence of SEQ ID NO: 112, aMTD501 has the sequence of SEQ ID NO:115, aMTD503 has the sequence of SEQ ID NO: 117, aMTD504 has thesequence of SEQ ID NO: 118, aMTD505 has the sequence of SEQ ID NO: 119,aMTD521 has the sequence of SEQ ID NO: 120, aMTD524 has the sequence ofSEQ ID NO: 122, aMTD525 has the sequence of SEQ ID NO: 123, aMTD541 hasthe sequence of SEQ ID NO: 124, aMTD542 has the sequence of SEQ ID NO:125, aMTD543 has the sequence of SEQ ID NO: 126, aMTD544 has thesequence of SEQ ID NO: 127, aMTD561 has the sequence of SEQ ID NO: 129,aMTD562 has the sequence of SEQ ID NO: 130, aMTD563 has the sequence ofSEQ ID NO: 131, aMTD564 has the sequence of SEQ ID NO: 132, aMTD565 hasthe sequence of SEQ ID NO: 133, aMTD582 has the sequence of SEQ ID NO:134.

In FIG. 7f , aMTD585 has the sequence of SEQ ID NO: 136, aMTD601 has thesequence of SEQ ID NO: 137, aMTD603 has the sequence of SEQ ID NO: 139,aMTD605 has the sequence of SEQ ID NO: 141, aMTD622 has the sequence ofSEQ ID NO: 142, aMTD623 has the sequence of SEQ ID NO: 143, aMTD645 hasthe sequence of SEQ ID NO: 146, aMTD661 has the sequence of SEQ ID NO:147, aMTD664 has the sequence of SEQ ID NO: 148, aMTD666 has thesequence of SEQ ID NO: 150, aMTD667 has the sequence of SEQ ID NO: 151,aMTD684 has the sequence of SEQ ID NO: 153, aMTD746 has the sequence ofSEQ ID NO: 167, aMTD763 has the sequence of SEQ ID NO: 169, aMTD764 hasthe sequence of SEQ ID NO: 170, aMTD766 has the sequence of SEQ ID NO:172, aMTD783 has the sequence of SEQ ID NO: 174, aMTD784 has thesequence of SEQ ID NO: 175, aMTD808 has the sequence of SEQ ID NO: 183,aMTD846 has the sequence of SEQ ID NO: 199, aMTD847 has the sequence ofSEQ ID NO: 200, aMTD848 has the sequence of SEQ ID NO: 201, aMTD849 hasthe sequence of SEQ ID NO: 202, aMTD850 has the sequence of SEQ ID NO:203.

In FIG. 7g , aMTD865 has the sequence of SEQ ID NO: 208, aMTD727 has thesequence of SEQ ID NO: 164, aMTD887 has the sequence of SEQ ID NO: 221,aMTD787 has the sequence of SEQ ID NO: 177, aMTD803 has the sequence ofSEQ ID NO: 179, aMTD805 has the sequence of SEQ ID NO: 180, aMTD824 hasthe sequence of SEQ ID NO: 187, aMTD825 has the sequence of SEQ ID NO:188, aMTD826 has the sequence of SEQ ID NO: 189, aMTD827 has thesequence of SEQ ID NO: 190, aMTD829 has the sequence of SEQ ID NO: 192,aMTD885 has the sequence of SEQ ID NO: 220, aMTD901 has the sequence ofSEQ ID NO: 231, aMTD912 has the sequence of SEQ ID NO: 240, aMTD891 hasthe sequence of SEQ ID NO: 224, aMTD895 has the sequence of SEQ ID NO:226, aMTD896 has the sequence of SEQ ID NO: 227, aMTD900 has thesequence of SEQ ID NO: 230, aMTD899 has the sequence of SEQ ID NO: 229,aMTD162 has the sequence of SEQ ID NO: 40, aMTD686 has the sequence ofSEQ ID NO: 155, aMTD225 has the sequence of SEQ ID NO: 55, aMTD899 hasthe sequence of SEQ ID NO: 229, aMTD897 has the sequence of SEQ ID NO:228.

In FIG. 7h , aMTD705 has the sequence of SEQ ID NO: 158, aMTD707 has thesequence of SEQ ID NO: 160, aMTD725 has the sequence of SEQ ID NO: 162,aMTD743 has the sequence of SEQ ID NO: 165, aMTD744 has the sequence ofSEQ ID NO: 166, aMTD747 has the sequence of SEQ ID NO: 168, aMTD830 hasthe sequence of SEQ ID NO: 193, aMTD832 has the sequence of SEQ ID NO:195, aMTD843 has the sequence of SEQ ID NO: 196, aMTD863 has thesequence of SEQ ID NO: 206, aMTD867 has the sequence of SEQ ID NO: 209,aMTD868 has the sequence of SEQ ID NO: 210, aMTD703 has the sequence ofSEQ ID NO: 157, aMTD724 has the sequence of SEQ ID NO: 161, aMTD726 hasthe sequence of SEQ ID NO: 163, aMTD786 has the sequence of SEQ ID NO:176, aMTD806 has the sequence of SEQ ID NO: 181, aMTD810 has thesequence of SEQ ID NO: 185, aMTD845 has the sequence of SEQ ID NO: 198,aMTD852 has the sequence of SEQ ID NO: 205, aMTD875 has the sequence ofSEQ ID NO: 213, aMTD877 has the sequence of SEQ ID NO: 214, aMTD902 hasthe sequence of SEQ ID NO: 232, aMTD907 has the sequence of SEQ ID NO:236.

In FIG. 7i , aMTD62 has the sequence of SEQ ID NO: 18, aMTD103 has thesequence of SEQ ID NO: 29, aMTD163 has the sequence of SEQ ID NO: 41,aMTD283 has the sequence of SEQ ID NO: 67, aMTD444 has the sequence ofSEQ ID NO: 103, aMTD484 has the sequence of SEQ ID NO: 113, aMTD502 hasthe sequence of SEQ ID NO: 116, aMTD602 has the sequence of SEQ ID NO:138, aMTD604 has the sequence of SEQ ID NO: 140, aMTD643 has thesequence of SEQ ID NO: 145, aMTD665 has the sequence of SEQ ID NO: 149,aMTD683 has the sequence of SEQ ID NO: 152, aMTD687 has the sequence ofSEQ ID NO: 156, aMTD706 has the sequence of SEQ ID NO: 159, aMTD765 hasthe sequence of SEQ ID NO: 171, aMTD767 has the sequence of SEQ ID NO:173, aMTD807 has the sequence of SEQ ID NO: 182, aMTD828 has thesequence of SEQ ID NO: 191, aMTD844 has the sequence of SEQ ID NO: 197,aMTD851 has the sequence of SEQ ID NO: 204, aMTD12 has the sequence ofSEQ ID NO: 7, aMTD81 has the sequence of SEQ ID NO: 22, aMTD104 has thesequence of SEQ ID NO: 30, aMTD904 has the sequence of SEQ ID NO: 233.

In FIG. 7j , aMTD165 has the sequence of SEQ ID NO: 43, aMTD205 has thesequence of SEQ ID NO: 50, aMTD241 has the sequence of SEQ ID NO: 56,aMTD264 has the sequence of SEQ ID NO: 63, aMTD301 has the sequence ofSEQ ID NO: 70, aMTD323 has the sequence of SEQ ID NO: 76, aMTD361 hasthe sequence of SEQ ID NO: 83, aMTD384 has the sequence of SEQ ID NO:90, aMTD421 has the sequence of SEQ ID NO: 97, aMTD445 has the sequenceof SEQ ID NO: 104, aMTD482 has the sequence of SEQ ID NO: 111, aMTD522has the sequence of SEQ ID NO: 121, aMTD545 has the sequence of SEQ IDNO: 128, aMTD583 has the sequence of SEQ ID NO: 135, aMTD625 has thesequence of SEQ ID NO: 144, aMTD870 has the sequence of SEQ ID NO: 211,aMTD788 has the sequence of SEQ ID NO: 178, aMTD864 has the sequence ofSEQ ID NO: 207, aMTD811 has the sequence of SEQ ID NO: 186, aMTD888 hasthe sequence of SEQ ID NO: 222, aMTD905 has the sequence of SEQ ID NO:234, aMTD324 has the sequence of SEQ ID NO: 77, aMTD809 has the sequenceof SEQ ID NO: 184, aMTD883 has the sequence of SEQ ID NO: 219.

In FIG. 7k , aMTD831 has the sequence of SEQ ID NO: 194, aMTD911 has thesequence of SEQ ID NO: 239, aMTD485 has the sequence of SEQ ID NO: 114,aMTD685 has the sequence of SEQ ID NO: 154, aMTD878 has the sequence ofSEQ ID NO: 215, aMTD42 has the sequence of SEQ ID NO: 14.

FIG. 8 shows Visualized Cell-Permeability of rPeptide-Fused RecombinantProteins. Cell-permeability of rPeptide-fused recombinant proteins wasvisualized by laser scanning confocal microscopy (LSM700 version).

In FIG. 8, rP692 has the sequence of SEQ ID NO: 907, rP26 has thesequence of SEQ ID NO: 895, rP113 has the sequence of SEQ ID NO: 891,rP466 has the sequence of SEQ ID NO: 897, rP167 has the sequence of SEQID NO: 898, rP97 has the sequence of SEQ ID NO: 936, rP390 has thesequence of SEQ ID NO: 909, rP426 has the sequence of SEQ ID NO: 900,rP214 has the sequence of SEQ ID NO: 937, rP68 has the sequence of SEQID NO: 913, rP39 has the sequence of SEQ ID NO: 941, rP934 has thesequence of SEQ ID NO: 888, rP938 has the sequence of SEQ ID NO: 916,rP329 has the sequence of SEQ ID NO: 917, rP606 has the sequence of SEQID NO: 901, rP49 has the sequence of SEQ ID NO: 918, rP139 has thesequence of SEQ ID NO: 944, rP772 has the sequence of SEQ ID NO: 919,rP921 has the sequence of SEQ ID NO: 931, rP66 has the sequence of SEQID NO: 902, rP693 has the sequence of SEQ ID NO: 922, rP18 has thesequence of SEQ ID NO: 945, rP16 has the sequence of SEQ ID NO: 890,rP227 has the sequence of SEQ ID NO: 904, rP17 has the sequence of SEQID NO: 905, rP67 has the sequence of SEQ ID NO: 906, rP635 has thesequence of SEQ ID NO: 947, rP29 has the sequence of SEQ ID NO: 924,rP57 has the sequence of SEQ ID NO: 949, rP700 has the sequence of SEQID NO: 951. FIGS. 9a to 9c show Relative Cell-Permeability of aMTD-FusedRecombinant Proteins Compared to Negative Control (rP38 (SEQ ID NO:952)). The FIG. shows graphs comparing the cell-permeability of therecombinant proteins fused to aMTDs and a negative control (A: rP38 (SEQID NO: 952)).

In FIG. 9a , rP38 has the sequence of SEQ ID NO: 952, aMTD899 has thesequence of SEQ ID NO: 229, aMTD908 has the sequence of SEQ ID NO: 237,aMTD910 has the sequence of SEQ ID NO: 238, aMTD810 has the sequence ofSEQ ID NO: 185, aMTD904 has the sequence of SEQ ID NO: 233, aMTD321 hasthe sequence of SEQ ID NO: 74, aMTD851 has the sequence of SEQ ID NO:204, aMTD911 has the sequence of SEQ ID NO: 239, aMTD852 has thesequence of SEQ ID NO: 205, aMTD803 has the sequence of SEQ ID NO: 179,aMTD888 has the sequence of SEQ ID NO: 222, aMTD825 has the sequence ofSEQ ID NO: 188, aMTD895 has the sequence of SEQ ID NO: 226, aMTD896 hasthe sequence of SEQ ID NO: 227, aMTD727 has the sequence of SEQ ID NO:164, aMTD603 has the sequence of SEQ ID NO: 139, aMTD847 has thesequence of SEQ ID NO: 200, aMTD826 has the sequence of SEQ ID NO: 189,aMTD724 has the sequence of SEQ ID NO: 161, aMTD564 has the sequence ofSEQ ID NO: 132, aMTD811 has the sequence of SEQ ID NO: 186, aMTD831 hasthe sequence of SEQ ID NO: 194, aMTD829 has the sequence of SEQ ID NO:192, aMTD891 has the sequence of SEQ ID NO: 224, aMTD905 has thesequence of SEQ ID NO: 234, aMTD564 has the sequence of SEQ ID NO: 132,aMTD124 has the sequence of SEQ ID NO: 34, aMTD827 has the sequence ofSEQ ID NO: 190, aMTD2 has the sequence of SEQ ID NO: 2, aMTD385 has thesequence of SEQ ID NO: 91, aMTD828 has the sequence of SEQ ID NO: 191,aMTD806 has the sequence of SEQ ID NO: 181, aMTD845 has the sequence ofSEQ ID NO: 198, aMTD882 has the sequence of SEQ ID NO: 218, aMTD545 hasthe sequence of SEQ ID NO: 128, aMTD161 has the sequence of SEQ ID NO:39, aMTD481 has the sequence of SEQ ID NO: 110, aMTD900 has the sequenceof SEQ ID NO: 230, aMTD223 has the sequence of SEQ ID NO: 53, aMTD824has the sequence of SEQ ID NO: 187, aMTD562 has the sequence of SEQ IDNO: 130, aMTD222 has the sequence of SEQ ID NO: 52, aMTD61 has thesequence of SEQ ID NO: 17, aMTD582 has the sequence of SEQ ID NO: 134,aMTD889 has the sequence of SEQ ID NO: 223, aMTD787 has the sequence ofSEQ ID NO: 177, aMTD703 has the sequence of SEQ ID NO: 157, aMTD705 hasthe sequence of SEQ ID NO: 158, aMTD885 has the sequence of SEQ ID NO:220, aMTD3 has the sequence of SEQ ID NO: 3, aMTD601 has the sequence ofSEQ ID NO: 137, aMTD843 has the sequence of SEQ ID NO: 196, aMTD403 hasthe sequence of SEQ ID NO: 94, aMTD544 has the sequence of SEQ ID NO:127, aMTD522 has the sequence of SEQ ID NO: 121, aMTD805 has thesequence of SEQ ID NO: 180, aMTD464 has the sequence of SEQ ID NO: 108,aMTD405 has the sequence of SEQ ID NO: 96, aMTD747 has the sequence ofSEQ ID NO: 168, aMTD501 has the sequence of SEQ ID NO: 115, aMTD661 hasthe sequence of SEQ ID NO: 147, aMTD786 has the sequence of SEQ ID NO:176, aMTD625 has the sequence of SEQ ID NO: 144, aMTD442 has thesequence of SEQ ID NO: 101, aMTD686 has the sequence of SEQ ID NO: 155,aMTD343 has the sequence of SEQ ID NO: 81, aMTD323 has the sequence ofSEQ ID NO: 76, aMTD461 has the sequence of SEQ ID NO: 105, aMTD21 hasthe sequence of SEQ ID NO: 9, aMTD404 has the sequence of SEQ ID NO: 95,aMTD261 has the sequence of SEQ ID NO: 60, aMTD524 has the sequence ofSEQ ID NO: 122, aMTD225 has the sequence of SEQ ID NO: 55, aMTD264 hasthe sequence of SEQ ID NO: 63, aMTD524 has the sequence of SEQ ID NO:122, aMTD225 has the sequence of SEQ ID NO: 55, aMTD264 has the sequenceof SEQ ID NO: 63, aMTD1 has the sequence of SEQ ID NO: 1, aMTD382 hasthe sequence of SEQ ID NO: 88, aMTD463 has the sequence of SEQ ID NO:107, aMTD322 has the sequence of SEQ ID NO: 75, aMTD503 has the sequenceof SEQ ID NO: 117, aMTD870 has the sequence of SEQ ID NO: 211, aMTD241has the sequence of SEQ ID NO: 56, aMTD726 has the sequence of SEQ IDNO: 163, aMTD341 has the sequence of SEQ ID NO: 79, aMTD542 has thesequence of SEQ ID NO: 125, aMTD361 has the sequence of SEQ ID NO: 83,aMTD224 has the sequence of SEQ ID NO: 54, aMTD482 has the sequence ofSEQ ID NO: 111.

In FIG. 9b , aMTD64 has the sequence of SEQ ID NO: 20, aMTD484 has thesequence of SEQ ID NO: 113, aMTD868 has the sequence of SEQ ID NO: 210,aMTD541 has the sequence of SEQ ID NO: 124, aMTD666 has the sequence ofSEQ ID NO: 150, aMTD665 has the sequence of SEQ ID NO: 149, aMTD363 hasthe sequence of SEQ ID NO: 84, aMTD242 has the sequence of SEQ ID NO:57, aMTD384 has the sequence of SEQ ID NO: 90, aMTD877 has the sequenceof SEQ ID NO: 214, aMTD863 has the sequence of SEQ ID NO: 206, aMTD525has the sequence of SEQ ID NO: 123, aMTD875 has the sequence of SEQ IDNO: 213, aMTD285 has the sequence of SEQ ID NO: 69, aMTD281 has thesequence of SEQ ID NO: 65, aMTD867 has the sequence of SEQ ID NO: 209,aMTD766 has the sequence of SEQ ID NO: 172, aMTD342 has the sequence ofSEQ ID NO: 80, aMTD881 has the sequence of SEQ ID NO: 217, aMTD505 hasthe sequence of SEQ ID NO: 119, aMTD763 has the sequence of SEQ ID NO:169, aMTD706 has the sequence of SEQ ID NO: 159, aMTD687 has thesequence of SEQ ID NO: 156, aMTD643 has the sequence of SEQ ID NO: 145,aMTD282 has the sequence of SEQ ID NO: 66, aMTD543 has the sequence ofSEQ ID NO: 126, aMTD325 has the sequence of SEQ ID NO: 78, aMTD846 hasthe sequence of SEQ ID NO: 199, aMTD383 has the sequence of SEQ ID NO:89, aMTD381 has the sequence of SEQ ID NO: 87, aMTD808 has the sequenceof SEQ ID NO: 183, aMTD865 has the sequence of SEQ ID NO: 208, aMTD725has the sequence of SEQ ID NO: 162, aMTD844 has the sequence of SEQ IDNO: 197, aMTD897 has the sequence of SEQ ID NO: 228, aMTD605 has thesequence of SEQ ID NO: 141, aMTD744 has the sequence of SEQ ID NO: 166,aMTD221 has the sequence of SEQ ID NO: 51, aMTD622 has the sequence ofSEQ ID NO: 142, aMTD401 has the sequence of SEQ ID NO: 92, aMTD324 hasthe sequence of SEQ ID NO: 77, aMTD878 has the sequence of SEQ ID NO:215, aMTD302 has the sequence of SEQ ID NO: 71, aMTD685 has the sequenceof SEQ ID NO: 154, aMTD848 has the sequence of SEQ ID NO: 201, aMTD602has the sequence of SEQ ID NO: 138, aMTD788 has the sequence of SEQ IDNO: 178, aMTD145 has the sequence of SEQ ID NO: 38, aMTD11 has thesequence of SEQ ID NO: 6, aMTD141 has the sequence of SEQ ID NO: 35,aMTD521 has the sequence of SEQ ID NO: 120, aMTD425 has the sequence ofSEQ ID NO: 100, aMTD365 has the sequence of SEQ ID NO: 86, aMTD263 hasthe sequence of SEQ ID NO: 62, aMTD345 has the sequence of SEQ ID NO:82, aMTD850 has the sequence of SEQ ID NO: 203, aMTD144 has the sequenceof SEQ ID NO: 37, aMTD767 has the sequence of SEQ ID NO: 173, aMTD185has the sequence of SEQ ID NO: 47, aMTD849 has the sequence of SEQ IDNO: 202, aMTD864 has the sequence of SEQ ID NO: 207, aMTD162 has thesequence of SEQ ID NO: 40, aMTD164 has the sequence of SEQ ID NO: 42,aMTD907 has the sequence of SEQ ID NO: 236, aMTD444 has the sequence ofSEQ ID NO: 103, aMTD443 has the sequence of SEQ ID NO: 102, aMTD901 hasthe sequence of SEQ ID NO: 231, aMTD887 has the sequence of SEQ ID NO:221, aMTD746 has the sequence of SEQ ID NO: 167, aMTD902 has thesequence of SEQ ID NO: 232, aMTD565 has the sequence of SEQ ID NO: 133,aMTD245 has the sequence of SEQ ID NO: 59, aMTD743 has the sequence ofSEQ ID NO: 165, aMTD464 has the sequence of SEQ ID NO: 108, aMTD104 hasthe sequence of SEQ ID NO: 30, aMTD707 has the sequence of SEQ ID NO:160, aMTD872 has the sequence of SEQ ID NO: 212, aMTD583 has thesequence of SEQ ID NO: 135, aMTD879 has the sequence of SEQ ID NO: 216,aMTD784 has the sequence of SEQ ID NO: 175, aMTD893 has the sequence ofSEQ ID NO: 225, aMTD13 has the sequence of SEQ ID NO: 8, aMTD809 has thesequence of SEQ ID NO: 184, aMTD445 has the sequence of SEQ ID NO: 104,aMTD81 has the sequence of SEQ ID NO: 22, aMTD667 has the sequence ofSEQ ID NO: 151, aMTD906 has the sequence of SEQ ID NO: 235, aMTD483 hasthe sequence of SEQ ID NO: 112, aMTD485 has the sequence of SEQ ID NO:114, aMTD421 has the sequence of SEQ ID NO: 97.

In FIG. 9c , aMTD585 has the sequence of SEQ ID NO: 136, aMTD424 has thesequence of SEQ ID NO: 99, aMTD364 has the sequence of SEQ ID NO: 85,aMTD402 has the sequence of SEQ ID NO: 93, aMTD462 has the sequence ofSEQ ID NO: 106, aMTD265 has the sequence of SEQ ID NO: 64, aMTD301 hasthe sequence of SEQ ID NO: 70, aMTD183 has the sequence of SEQ ID NO:45, aMTD243 has the sequence of SEQ ID NO: 58, aMTD664 has the sequenceof SEQ ID NO: 148, aMTD783 has the sequence of SEQ ID NO: 174, aMTD502has the sequence of SEQ ID NO: 116, aMTD262 has the sequence of SEQ IDNO: 61, aMTD683 has the sequence of SEQ ID NO: 152, aMTD830 has thesequence of SEQ ID NO: 193, aMTD764 has the sequence of SEQ ID NO: 170,aMTD807 has the sequence of SEQ ID NO: 182, aMTD184 has the sequence ofSEQ ID NO: 46, aMTD305 has the sequence of SEQ ID NO: 73, aMTD101 hasthe sequence of SEQ ID NO: 27, aMTD304 has the sequence of SEQ ID NO:72, aMTD604 has the sequence of SEQ ID NO: 140, aMTD645 has the sequenceof SEQ ID NO: 146, aMTD201 has the sequence of SEQ ID NO: 48, aMTD163has the sequence of SEQ ID NO: 41, aMTD832 has the sequence of SEQ IDNO: 195, aMTD182 has the sequence of SEQ ID NO: 44, aMTD23 has thesequence of SEQ ID NO: 11, aMTD105 has the sequence of SEQ ID NO: 31,aMTD561 has the sequence of SEQ ID NO: 129, aMTD765 has the sequence ofSEQ ID NO: 171, aMTD684 has the sequence of SEQ ID NO: 153, aMTD143 hasthe sequence of SEQ ID NO: 36, aMTD504 has the sequence of SEQ ID NO:118, aMTD22 has the sequence of SEQ ID NO: 10, aMTD5 has the sequence ofSEQ ID NO: 5, aMTD283 has the sequence of SEQ ID NO: 67, aMTD65 has thesequence of SEQ ID NO: 21, aMTD883 has the sequence of SEQ ID NO: 219,aMTD123 has the sequence of SEQ ID NO: 33, aMTD284 has the sequence ofSEQ ID NO: 68, aMTD205 has the sequence of SEQ ID NO: 50, aMTD42 has thesequence of SEQ ID NO: 14, aMTD121 has the sequence of SEQ ID NO: 32,aMTD25 has the sequence of SEQ ID NO: 13, aMTD24 has the sequence of SEQID NO: 12, aMTD204 has the sequence of SEQ ID NO: 49, aMTD12 has thesequence of SEQ ID NO: 7, aMTD43 has the sequence of SEQ ID NO: 15,aMTD103 has the sequence of SEQ ID NO: 29, aMTD82 has the sequence ofSEQ ID NO: 23, aMTD4 has the sequence of SEQ ID NO:4, aMTD85 has thesequence of SEQ ID NO: 26, aMTD63 has the sequence of SEQ ID NO: 19,aMTD44 has the sequence of SEQ ID NO: 16, aMTD84 has the sequence of SEQID NO: 25, aMTD62 has the sequence of SEQ ID NO: 18, aMTD83 has thesequence of SEQ ID NO: 24, aMTD102 has the sequence of SEQ ID NO: 28,aMTD623 has the sequence of SEQ ID NO: 143. FIGS. 10a to 10c showRelative Cell-Permeability of aMTD-Fused Recombinant Proteins Comparedto Reference CPP (MTM12 (SEQ ID NO: 953)). The FIG. shows graphscomparing the cell-permeability of the recombinant proteins fused toaMTDs and a reference CPP (MTM12 (SEQ ID NO: 953)).

In FIG. 10a , MTM12 has the sequence of SEQ ID NO: 953, aMTD899 has thesequence of SEQ ID NO: 229, aMTD908 has the sequence of SEQ ID NO: 237,aMTD910 has the sequence of SEQ ID NO: 238, aMTD810 has the sequence ofSEQ ID NO: 185, aMTD904 has the sequence of SEQ ID NO: 233, aMTD321 hasthe sequence of SEQ ID NO: 74, aMTD851 has the sequence of SEQ ID NO:204, aMTD911 has the sequence of SEQ ID NO: 239, aMTD852 has thesequence of SEQ ID NO: 205, aMTD803 has the sequence of SEQ ID NO: 179,aMTD888 has the sequence of SEQ ID NO: 222, aMTD825 has the sequence ofSEQ ID NO: 188, aMTD895 has the sequence of SEQ ID NO: 226, aMTD896 hasthe sequence of SEQ ID NO: 227, aMTD727 has the sequence of SEQ ID NO:164, aMTD603 has the sequence of SEQ ID NO: 139, aMTD847 has thesequence of SEQ ID NO: 200, aMTD826 has the sequence of SEQ ID NO: 189,aMTD724 has the sequence of SEQ ID NO: 161, aMTD564 has the sequence ofSEQ ID NO: 132, aMTD811 has the sequence of SEQ ID NO: 186, aMTD831 hasthe sequence of SEQ ID NO: 194, aMTD829 has the sequence of SEQ ID NO:192, aMTD891 has the sequence of SEQ ID NO: 224, aMTD905 has thesequence of SEQ ID NO: 234, aMTD564 has the sequence of SEQ ID NO: 132,aMTD124 has the sequence of SEQ ID NO: 34, aMTD827 has the sequence ofSEQ ID NO: 190, aMTD2 has the sequence of SEQ ID NO: 2, aMTD385 has thesequence of SEQ ID NO: 91, aMTD828 has the sequence of SEQ ID NO: 191,aMTD806 has the sequence of SEQ ID NO: 181, aMTD845 has the sequence ofSEQ ID NO: 198, aMTD882 has the sequence of SEQ ID NO: 218, aMTD545 hasthe sequence of SEQ ID NO: 128, aMTD161 has the sequence of SEQ ID NO:39, aMTD481 has the sequence of SEQ ID NO: 110, aMTD900 has the sequenceof SEQ ID NO: 230, aMTD223 has the sequence of SEQ ID NO: 53, aMTD824has the sequence of SEQ ID NO: 187, aMTD562 has the sequence of SEQ IDNO: 130, aMTD222 has the sequence of SEQ ID NO: 52, aMTD61 has thesequence of SEQ ID NO: 17, aMTD582 has the sequence of SEQ ID NO: 134,aMTD889 has the sequence of SEQ ID NO: 223, aMTD787 has the sequence ofSEQ ID NO: 177, aMTD703 has the sequence of SEQ ID NO: 157, aMTD705 hasthe sequence of SEQ ID NO: 158, aMTD885 has the sequence of SEQ ID NO:220, aMTD3 has the sequence of SEQ ID NO: 3, aMTD601 has the sequence ofSEQ ID NO: 137, aMTD843 has the sequence of SEQ ID NO: 196, aMTD403 hasthe sequence of SEQ ID NO: 94, aMTD544 has the sequence of SEQ ID NO:127, aMTD522 has the sequence of SEQ ID NO: 121, aMTD805 has thesequence of SEQ ID NO: 180, aMTD464 has the sequence of SEQ ID NO: 108,aMTD405 has the sequence of SEQ ID NO: 96, aMTD747 has the sequence ofSEQ ID NO: 168, aMTD501 has the sequence of SEQ ID NO: 115, aMTD661 hasthe sequence of SEQ ID NO: 147, aMTD786 has the sequence of SEQ ID NO:176, aMTD625 has the sequence of SEQ ID NO: 144, aMTD442 has thesequence of SEQ ID NO: 101, aMTD686 has the sequence of SEQ ID NO: 155,aMTD343 has the sequence of SEQ ID NO: 81, aMTD323 has the sequence ofSEQ ID NO: 76, aMTD461 has the sequence of SEQ ID NO: 105, aMTD21 hasthe sequence of SEQ ID NO: 9, aMTD404 has the sequence of SEQ ID NO: 95,aMTD261 has the sequence of SEQ ID NO: 60, aMTD524 has the sequence ofSEQ ID NO: 122, aMTD225 has the sequence of SEQ ID NO: 55, aMTD264 hasthe sequence of SEQ ID NO: 63, aMTD524 has the sequence of SEQ ID NO:122, aMTD225 has the sequence of SEQ ID NO: 55, aMTD264 has the sequenceof SEQ ID NO: 63, aMTD1 has the sequence of SEQ ID NO: 1, aMTD382 hasthe sequence of SEQ ID NO: 88, aMTD463 has the sequence of SEQ ID NO:107, aMTD322 has the sequence of SEQ ID NO: 75, aMTD503 has the sequenceof SEQ ID NO: 117, aMTD870 has the sequence of SEQ ID NO: 211, aMTD241has the sequence of SEQ ID NO: 56, aMTD726 has the sequence of SEQ IDNO: 163, aMTD341 has the sequence of SEQ ID NO: 79, aMTD542 has thesequence of SEQ ID NO: 125, aMTD361 has the sequence of SEQ ID NO: 83,aMTD224 has the sequence of SEQ ID NO: 54, aMTD482 has the sequence ofSEQ ID NO: 111.

In FIG. 10b , aMTD64 has the sequence of SEQ ID NO: 20, aMTD484 has thesequence of SEQ ID NO: 113, aMTD868 has the sequence of SEQ ID NO: 210,aMTD666 has the sequence of SEQ ID NO: 150, aMTD541 has the sequence ofSEQ ID NO: 124, aMTD665 has the sequence of SEQ ID NO: 149, aMTD363 hasthe sequence of SEQ ID NO: 84, aMTD242 has the sequence of SEQ ID NO:57, aMTD384 has the sequence of SEQ ID NO: 90, aMTD877 has the sequenceof SEQ ID NO: 214, aMTD863 has the sequence of SEQ ID NO: 206, aMTD525has the sequence of SEQ ID NO: 123, aMTD875 has the sequence of SEQ IDNO: 213, aMTD285 has the sequence of SEQ ID NO: 69, aMTD281 has thesequence of SEQ ID NO: 65, aMTD867 has the sequence of SEQ ID NO: 209,aMTD766 has the sequence of SEQ ID NO: 172, aMTD881 has the sequence ofSEQ ID NO: 217, aMTD342 has the sequence of SEQ ID NO: 80, aMTD505 hasthe sequence of SEQ ID NO: 119, aMTD643 has the sequence of SEQ ID NO:145, aMTD282 has the sequence of SEQ ID NO: 66, aMTD325 has the sequenceof SEQ ID NO: 78, aMTD543 has the sequence of SEQ ID NO: 126, aMTD383has the sequence of SEQ ID NO: 89, aMTD381 has the sequence of SEQ IDNO: 87, aMTD763 has the sequence of SEQ ID NO: 169, aMTD687 has thesequence of SEQ ID NO: 156, aMTD706 has the sequence of SEQ ID NO: 159,aMTD846 has the sequence of SEQ ID NO: 199, aMTD808 has the sequence ofSEQ ID NO: 183, aMTD865 has the sequence of SEQ ID NO: 208, aMTD725 hasthe sequence of SEQ ID NO: 162, aMTD844 has the sequence of SEQ ID NO:197, aMTD897 has the sequence of SEQ ID NO: 228, aMTD605 has thesequence of SEQ ID NO: 141, aMTD744 has the sequence of SEQ ID NO: 166,aMTD221 has the sequence of SEQ ID NO: 51, aMTD622 has the sequence ofSEQ ID NO: 142, aMTD401 has the sequence of SEQ ID NO: 92, aMTD324 hasthe sequence of SEQ ID NO: 77, aMTD878 has the sequence of SEQ ID NO:215, aMTD302 has the sequence of SEQ ID NO: 71, aMTD848 has the sequenceof SEQ ID NO: 201, aMTD788 has the sequence of SEQ ID NO: 178, aMTD145has the sequence of SEQ ID NO: 38, aMTD11 has the sequence of SEQ ID NO:6, aMTD425 has the sequence of SEQ ID NO: 100, aMTD141 has the sequenceof SEQ ID NO: 35, aMTD521 has the sequence of SEQ ID NO: 120, aMTD365has the sequence of SEQ ID NO: 86, aMTD263 has the sequence of SEQ IDNO: 62, aMTD345 has the sequence of SEQ ID NO: 82, aMTD685 has thesequence of SEQ ID NO: 154, aMTD850 has the sequence of SEQ ID NO: 203,aMTD144 has the sequence of SEQ ID NO: 37, aMTD185 has the sequence ofSEQ ID NO: 47, aMTD767 has the sequence of SEQ ID NO: 173, aMTD849 hasthe sequence of SEQ ID NO: 202, aMTD162 has the sequence of SEQ ID NO:40, aMTD164 has the sequence of SEQ ID NO: 42, aMTD907 has the sequenceof SEQ ID NO: 236, aMTD444 has the sequence of SEQ ID NO: 103, aMTD443has the sequence of SEQ ID NO: 102, aMTD901 has the sequence of SEQ IDNO: 231, aMTD887 has the sequence of SEQ ID NO: 221, aMTD902 has thesequence of SEQ ID NO: 232, aMTD565 has the sequence of SEQ ID NO: 133,aMTD245 has the sequence of SEQ ID NO: 59, aMTD743 has the sequence ofSEQ ID NO: 165, aMTD465 has the sequence of SEQ ID NO: 109, aMTD707 hasthe sequence of SEQ ID NO: 160, aMTD104 has the sequence of SEQ ID NO:30, aMTD872 has the sequence of SEQ ID NO: 212, aMTD864 has the sequenceof SEQ ID NO: 207, aMTD583 has the sequence of SEQ ID NO: 135, aMTD879has the sequence of SEQ ID NO: 216, aMTD784 has the sequence of SEQ IDNO: 175, aMTD893 has the sequence of SEQ ID NO: 225, aMTD809 has thesequence of SEQ ID NO: 184, aMTD13 has the sequence of SEQ ID NO: 8,aMTD445 has the sequence of SEQ ID NO: 104, aMTD667 has the sequence ofSEQ ID NO: 151, aMTD81 has the sequence of SEQ ID NO: 22, aMTD906 hasthe sequence of SEQ ID NO: 235, aMTD483 has the sequence of SEQ ID NO:112, aMTD421 has the sequence of SEQ ID NO: 97, aMTD746 has the sequenceof SEQ ID NO: 167.

In FIG. 10c , aMTD585 has the sequence of SEQ ID NO: 136, aMTD424 hasthe sequence of SEQ ID NO: 99, aMTD364 has the sequence of SEQ ID NO:85, aMTD402 has the sequence of SEQ ID NO: 93, aMTD462 has the sequenceof SEQ ID NO: 106, aMTD265 has the sequence of SEQ ID NO: 64, aMTD402has the sequence of SEQ ID NO: 93, aMTD462 has the sequence of SEQ IDNO: 106, aMTD265 has the sequence of SEQ ID NO: 64, aMTD301 has thesequence of SEQ ID NO: 70, aMTD183 has the sequence of SEQ ID NO: 45,aMTD243 has the sequence of SEQ ID NO: 58, aMTD664 has the sequence ofSEQ ID NO: 148, aMTD783 has the sequence of SEQ ID NO: 174, aMTD502 hasthe sequence of SEQ ID NO: 116, aMTD262 has the sequence of SEQ ID NO:61, aMTD807 has the sequence of SEQ ID NO: 182, aMTD683 has the sequenceof SEQ ID NO: 152, aMTD184 has the sequence of SEQ ID NO: 46, aMTD305has the sequence of SEQ ID NO: 73, aMTD101 has the sequence of SEQ IDNO: 27, aMTD304 has the sequence of SEQ ID NO: 72, aMTD645 has thesequence of SEQ ID NO: 146, aMTD201 has the sequence of SEQ ID NO: 48,aMTD832 has the sequence of SEQ ID NO: 195, aMTD182 has the sequence ofSEQ ID NO: 44, aMTD23 has the sequence of SEQ ID NO: 11, aMTD105 has thesequence of SEQ ID NO: 31, aMTD561 has the sequence of SEQ ID NO: 129,aMTD604 has the sequence of SEQ ID NO: 140, aMTD163 has the sequence ofSEQ ID NO: 41, aMTD765 has the sequence of SEQ ID NO: 171, aMTD684 hasthe sequence of SEQ ID NO: 153, aMTD143 has the sequence of SEQ ID NO:36, aMTD504 has the sequence of SEQ ID NO: 118, aMTD22 has the sequenceof SEQ ID NO: 10, aMTD5 has the sequence of SEQ ID NO: 5, aMTD283 hasthe sequence of SEQ ID NO: 67, aMTD65 has the sequence of SEQ ID NO: 21,aMTD883 has the sequence of SEQ ID NO: 219, aMTD123 has the sequence ofSEQ ID NO: 33, aMTD284 has the sequence of SEQ ID NO: 68, aMTD205 hasthe sequence of SEQ ID NO: 50, aMTD42 has the sequence of SEQ ID NO: 14,aMTD121 has the sequence of SEQ ID NO: 32, aMTD25 has the sequence ofSEQ ID NO: 13, aMTD24 has the sequence of SEQ ID NO: 12, aMTD204 has thesequence of SEQ ID NO: 49, aMTD12 has the sequence of SEQ ID NO: 7,aMTD43 has the sequence of SEQ ID NO: 15, aMTD103 has the sequence ofSEQ ID NO: 29, aMTD82 has the sequence of SEQ ID NO: 23, aMTD4 has thesequence of SEQ ID NO:4, aMTD85 has the sequence of SEQ ID NO: 26,aMTD63 has the sequence of SEQ ID NO: 19, aMTD44 has the sequence of SEQID NO: 16, aMTD84 has the sequence of SEQ ID NO: 25, aMTD62 has thesequence of SEQ ID NO: 18, aMTD83 has the sequence of SEQ ID NO: 24,aMTD102 has the sequence of SEQ ID NO: 28, aMTD623 has the sequence ofSEQ ID NO: 143. FIGS. 11a to 11c show Relative Cell-Permeability ofaMTD-Fused Recombinant Proteins Compared to Reference CPP (MTD85 (SEQ IDNO: 954)). The FIG. shows graphs comparing the cell-permeability of therecombinant proteins fused to aMTDs and a reference CPP (MTD85 (SEQ IDNO: 954)).

In FIG. 11a , MTD85 has the sequence of SEQ ID NO: 954, aMTD899 has thesequence of SEQ ID NO: 229, aMTD908 has the sequence of SEQ ID NO: 237,aMTD910 has the sequence of SEQ ID NO: 238, aMTD810 has the sequence ofSEQ ID NO: 185, aMTD904 has the sequence of SEQ ID NO: 233, aMTD321 hasthe sequence of SEQ ID NO: 74, aMTD851 has the sequence of SEQ ID NO:204, aMTD911 has the sequence of SEQ ID NO: 239, aMTD852 has thesequence of SEQ ID NO: 205, aMTD803 has the sequence of SEQ ID NO: 179,aMTD888 has the sequence of SEQ ID NO: 222, aMTD825 has the sequence ofSEQ ID NO: 188, aMTD895 has the sequence of SEQ ID NO: 226, aMTD896 hasthe sequence of SEQ ID NO: 227, aMTD727 has the sequence of SEQ ID NO:164, aMTD603 has the sequence of SEQ ID NO: 139, aMTD847 has thesequence of SEQ ID NO: 200, aMTD826 has the sequence of SEQ ID NO: 189,aMTD724 has the sequence of SEQ ID NO: 161, aMTD564 has the sequence ofSEQ ID NO: 132, aMTD811 has the sequence of SEQ ID NO: 186, aMTD831 hasthe sequence of SEQ ID NO: 194, aMTD829 has the sequence of SEQ ID NO:192, aMTD891 has the sequence of SEQ ID NO: 224, aMTD905 has thesequence of SEQ ID NO: 234, aMTD564 has the sequence of SEQ ID NO: 132,aMTD124 has the sequence of SEQ ID NO: 34, aMTD827 has the sequence ofSEQ ID NO: 190, aMTD2 has the sequence of SEQ ID NO: 2, aMTD385 has thesequence of SEQ ID NO: 91, aMTD828 has the sequence of SEQ ID NO: 191,aMTD806 has the sequence of SEQ ID NO: 181, aMTD845 has the sequence ofSEQ ID NO: 198, aMTD882 has the sequence of SEQ ID NO: 218, aMTD545 hasthe sequence of SEQ ID NO: 128, aMTD161 has the sequence of SEQ ID NO:39, aMTD481 has the sequence of SEQ ID NO: 110, aMTD900 has the sequenceof SEQ ID NO: 230, aMTD223 has the sequence of SEQ ID NO: 53, aMTD824has the sequence of SEQ ID NO: 187, aMTD562 has the sequence of SEQ IDNO: 130, aMTD222 has the sequence of SEQ ID NO: 52, aMTD61 has thesequence of SEQ ID NO: 17, aMTD582 has the sequence of SEQ ID NO: 134,aMTD889 has the sequence of SEQ ID NO: 223, aMTD787 has the sequence ofSEQ ID NO: 177, aMTD703 has the sequence of SEQ ID NO: 157, aMTD705 hasthe sequence of SEQ ID NO: 158, aMTD885 has the sequence of SEQ ID NO:220, aMTD3 has the sequence of SEQ ID NO: 3, aMTD601 has the sequence ofSEQ ID NO: 137, aMTD843 has the sequence of SEQ ID NO: 196, aMTD403 hasthe sequence of SEQ ID NO: 94, aMTD544 has the sequence of SEQ ID NO:127, aMTD522 has the sequence of SEQ ID NO: 121, aMTD805 has thesequence of SEQ ID NO: 180, aMTD464 has the sequence of SEQ ID NO: 108,aMTD405 has the sequence of SEQ ID NO: 96, aMTD747 has the sequence ofSEQ ID NO: 168, aMTD501 has the sequence of SEQ ID NO: 115, aMTD661 hasthe sequence of SEQ ID NO: 147, aMTD786 has the sequence of SEQ ID NO:176, aMTD625 has the sequence of SEQ ID NO: 144, aMTD442 has thesequence of SEQ ID NO: 101, aMTD686 has the sequence of SEQ ID NO: 155,aMTD343 has the sequence of SEQ ID NO: 81, aMTD323 has the sequence ofSEQ ID NO: 76, aMTD461 has the sequence of SEQ ID NO: 105, aMTD21 hasthe sequence of SEQ ID NO: 9, aMTD404 has the sequence of SEQ ID NO: 95,aMTD261 has the sequence of SEQ ID NO: 60, aMTD524 has the sequence ofSEQ ID NO: 122, aMTD225 has the sequence of SEQ ID NO: 55, aMTD264 hasthe sequence of SEQ ID NO: 63, aMTD524 has the sequence of SEQ ID NO:122, aMTD225 has the sequence of SEQ ID NO: 55, aMTD264 has the sequenceof SEQ ID NO: 63, aMTD1 has the sequence of SEQ ID NO: 1, aMTD382 hasthe sequence of SEQ ID NO: 88, aMTD463 has the sequence of SEQ ID NO:107, aMTD322 has the sequence of SEQ ID NO: 75, aMTD503 has the sequenceof SEQ ID NO: 117, aMTD870 has the sequence of SEQ ID NO: 211, aMTD241has the sequence of SEQ ID NO: 56, aMTD726 has the sequence of SEQ IDNO: 163, aMTD341 has the sequence of SEQ ID NO: 79, aMTD542 has thesequence of SEQ ID NO: 125, aMTD361 has the sequence of SEQ ID NO: 83,aMTD224 has the sequence of SEQ ID NO: 54, aMTD482 has the sequence ofSEQ ID NO: 111.

In FIG. 11b , aMTD64 has the sequence of SEQ ID NO: 20, aMTD484 has thesequence of SEQ ID NO: 113, aMTD868 has the sequence of SEQ ID NO: 210,aMTD541 has the sequence of SEQ ID NO: 124, aMTD666 has the sequence ofSEQ ID NO: 150, aMTD665 has the sequence of SEQ ID NO: 149, aMTD363 hasthe sequence of SEQ ID NO: 84, aMTD242 has the sequence of SEQ ID NO:57, aMTD384 has the sequence of SEQ ID NO: 90, aMTD877 has the sequenceof SEQ ID NO: 214, aMTD863 has the sequence of SEQ ID NO: 206, aMTD525has the sequence of SEQ ID NO: 123, aMTD875 has the sequence of SEQ IDNO: 213, aMTD285 has the sequence of SEQ ID NO: 69, aMTD281 has thesequence of SEQ ID NO: 65, aMTD867 has the sequence of SEQ ID NO: 209,aMTD766 has the sequence of SEQ ID NO: 172, aMTD342 has the sequence ofSEQ ID NO: 80, aMTD881 has the sequence of SEQ ID NO: 217, aMTD505 hasthe sequence of SEQ ID NO: 119, aMTD763 has the sequence of SEQ ID NO:169, aMTD706 has the sequence of SEQ ID NO: 159, aMTD687 has thesequence of SEQ ID NO: 156, aMTD643 has the sequence of SEQ ID NO: 145,aMTD282 has the sequence of SEQ ID NO: 66, aMTD543 has the sequence ofSEQ ID NO: 126, aMTD325 has the sequence of SEQ ID NO: 78, aMTD846 hasthe sequence of SEQ ID NO: 199, aMTD383 has the sequence of SEQ ID NO:89, aMTD381 has the sequence of SEQ ID NO: 87, aMTD808 has the sequenceof SEQ ID NO: 183, aMTD865 has the sequence of SEQ ID NO: 208, aMTD725has the sequence of SEQ ID NO: 162, aMTD844 has the sequence of SEQ IDNO: 197, aMTD897 has the sequence of SEQ ID NO: 228, aMTD605 has thesequence of SEQ ID NO: 141, aMTD744 has the sequence of SEQ ID NO: 166,aMTD221 has the sequence of SEQ ID NO: 51, aMTD622 has the sequence ofSEQ ID NO: 142, aMTD401 has the sequence of SEQ ID NO: 92, aMTD324 hasthe sequence of SEQ ID NO: 77, aMTD878 has the sequence of SEQ ID NO:215, aMTD302 has the sequence of SEQ ID NO: 71, aMTD685 has the sequenceof SEQ ID NO: 154, aMTD848 has the sequence of SEQ ID NO: 201, aMTD602has the sequence of SEQ ID NO: 138, aMTD788 has the sequence of SEQ IDNO: 178, aMTD145 has the sequence of SEQ ID NO: 38, aMTD11 has thesequence of SEQ ID NO: 6, aMTD141 has the sequence of SEQ ID NO: 35,aMTD521 has the sequence of SEQ ID NO: 120, aMTD425 has the sequence ofSEQ ID NO: 100, aMTD365 has the sequence of SEQ ID NO: 86, aMTD263 hasthe sequence of SEQ ID NO: 62, aMTD345 has the sequence of SEQ ID NO:82, aMTD850 has the sequence of SEQ ID NO: 203, aMTD144 has the sequenceof SEQ ID NO: 37, aMTD767 has the sequence of SEQ ID NO: 173, aMTD185has the sequence of SEQ ID NO: 47, aMTD849 has the sequence of SEQ IDNO: 202, aMTD864 has the sequence of SEQ ID NO: 207, aMTD162 has thesequence of SEQ ID NO: 40, aMTD164 has the sequence of SEQ ID NO: 42,aMTD907 has the sequence of SEQ ID NO: 236, aMTD444 has the sequence ofSEQ ID NO: 103, aMTD443 has the sequence of SEQ ID NO: 102, aMTD901 hasthe sequence of SEQ ID NO: 231, aMTD887 has the sequence of SEQ ID NO:221, aMTD746 has the sequence of SEQ ID NO: 167, aMTD902 has thesequence of SEQ ID NO: 232, aMTD565 has the sequence of SEQ ID NO: 133,aMTD245 has the sequence of SEQ ID NO: 59, aMTD743 has the sequence ofSEQ ID NO: 165, aMTD464 has the sequence of SEQ ID NO: 108, aMTD104 hasthe sequence of SEQ ID NO: 30, aMTD707 has the sequence of SEQ ID NO:160, aMTD872 has the sequence of SEQ ID NO: 212, aMTD583 has thesequence of SEQ ID NO: 135, aMTD879 has the sequence of SEQ ID NO: 216,aMTD784 has the sequence of SEQ ID NO: 175, aMTD893 has the sequence ofSEQ ID NO: 225, aMTD13 has the sequence of SEQ ID NO: 8, aMTD809 has thesequence of SEQ ID NO: 184, aMTD445 has the sequence of SEQ ID NO: 104,aMTD81 has the sequence of SEQ ID NO: 22, aMTD667 has the sequence ofSEQ ID NO: 151, aMTD906 has the sequence of SEQ ID NO: 235, aMTD483 hasthe sequence of SEQ ID NO: 112, aMTD485 has the sequence of SEQ ID NO:114, aMTD421 has the sequence of SEQ ID NO: 97.

In FIG. 11c , aMTD585 has the sequence of SEQ ID NO: 136, aMTD424 hasthe sequence of SEQ ID NO: 99, aMTD364 has the sequence of SEQ ID NO:85, aMTD402 has the sequence of SEQ ID NO: 93, aMTD462 has the sequenceof SEQ ID NO: 106, aMTD265 has the sequence of SEQ ID NO: 64, aMTD301has the sequence of SEQ ID NO: 70, aMTD183 has the sequence of SEQ IDNO: 45, aMTD243 has the sequence of SEQ ID NO: 58, aMTD664 has thesequence of SEQ ID NO: 148, aMTD783 has the sequence of SEQ ID NO: 174,aMTD502 has the sequence of SEQ ID NO: 116, aMTD262 has the sequence ofSEQ ID NO: 61, aMTD683 has the sequence of SEQ ID NO: 152, aMTD830 hasthe sequence of SEQ ID NO: 193, aMTD764 has the sequence of SEQ ID NO:170, aMTD807 has the sequence of SEQ ID NO: 182, aMTD184 has thesequence of SEQ ID NO: 46, aMTD305 has the sequence of SEQ ID NO: 73,aMTD101 has the sequence of SEQ ID NO: 27, aMTD304 has the sequence ofSEQ ID NO: 72, aMTD604 has the sequence of SEQ ID NO: 140, aMTD645 hasthe sequence of SEQ ID NO: 146, aMTD201 has the sequence of SEQ ID NO:48, aMTD163 has the sequence of SEQ ID NO: 41, aMTD832 has the sequenceof SEQ ID NO: 195, aMTD182 has the sequence of SEQ ID NO: 44, aMTD23 hasthe sequence of SEQ ID NO: 11, aMTD105 has the sequence of SEQ ID NO:31, aMTD561 has the sequence of SEQ ID NO: 129, aMTD765 has the sequenceof SEQ ID NO: 171, aMTD684 has the sequence of SEQ ID NO: 153, aMTD143has the sequence of SEQ ID NO: 36, aMTD504 has the sequence of SEQ IDNO: 118, aMTD22 has the sequence of SEQ ID NO: 10, aMTD5 has thesequence of SEQ ID NO: 5, aMTD283 has the sequence of SEQ ID NO: 67,aMTD65 has the sequence of SEQ ID NO: 21, aMTD883 has the sequence ofSEQ ID NO: 219, aMTD123 has the sequence of SEQ ID NO: 33, aMTD284 hasthe sequence of SEQ ID NO: 68, aMTD205 has the sequence of SEQ ID NO:50, aMTD42 has the sequence of SEQ ID NO: 14, aMTD121 has the sequenceof SEQ ID NO: 32, aMTD25 has the sequence of SEQ ID NO: 13, aMTD24 hasthe sequence of SEQ ID NO: 12, aMTD204 has the sequence of SEQ ID NO:49, aMTD12 has the sequence of SEQ ID NO: 7, aMTD43 has the sequence ofSEQ ID NO: 15, aMTD103 has the sequence of SEQ ID NO: 29, aMTD82 has thesequence of SEQ ID NO: 23, aMTD4 has the sequence of SEQ ID NO:4, aMTD85has the sequence of SEQ ID NO: 26, aMTD63 has the sequence of SEQ ID NO:19, aMTD44 has the sequence of SEQ ID NO: 16, aMTD84 has the sequence ofSEQ ID NO: 25, aMTD62 has the sequence of SEQ ID NO: 18, aMTD83 has thesequence of SEQ ID NO: 24, aMTD102 has the sequence of SEQ ID NO: 28,aMTD623 has the sequence of SEQ ID NO: 143. FIG. 12 shows RelativeCell-Permeability of rPeptide-Mediated Recombinant Proteins Compared toAverage that of aMTDs. The FIG. shows graphs comparing thecell-permeability of the recombinant proteins fused to rPeptides andthat (average value: aMTD AVE) of aMTDs.

In FIG. 12, rP692 has the sequence of SEQ ID NO: 907, rP26 has thesequence of SEQ ID NO: 895, rP113 has the sequence of SEQ ID NO: 891,rP466 has the sequence of SEQ ID NO: 897, rP167 has the sequence of SEQID NO: 898, rP97 has the sequence of SEQ ID NO: 936, rP390 has thesequence of SEQ ID NO: 909, rP426 has the sequence of SEQ ID NO: 900,rP214 has the sequence of SEQ ID NO: 937, rP68 has the sequence of SEQID NO: 913, rP39 has the sequence of SEQ ID NO: 941, rP934 has thesequence of SEQ ID NO: 888, rP938 has the sequence of SEQ ID NO: 916,rP329 has the sequence of SEQ ID NO: 917, rP606 has the sequence of SEQID NO: 901, rP49 has the sequence of SEQ ID NO: 918, rP139 has thesequence of SEQ ID NO: 944, rP772 has the sequence of SEQ ID NO: 919,rP921 has the sequence of SEQ ID NO: 931, rP66 has the sequence of SEQID NO: 902, rP693 has the sequence of SEQ ID NO: 922, rP18 has thesequence of SEQ ID NO: 945, rP16 has the sequence of SEQ ID NO: 890,rP227 has the sequence of SEQ ID NO: 904, rP17 has the sequence of SEQID NO: 905, rP67 has the sequence of SEQ ID NO: 906, rP227 has thesequence of SEQ ID NO: 904, rP17 has the sequence of SEQ ID NO: 905,rP67 has the sequence of SEQ ID NO: 906, rP635 has the sequence of SEQID NO: 947, rP29 has the sequence of SEQ ID NO: 924, rP57 has thesequence of SEQ ID NO: 949, rP700 has the sequence of SEQ ID NO: 951,rP38 has the sequence of SEQ ID NO: 952. FIGS. 13a and 13b showAssociation of Cell-Permeability with Amino Acid Composition in aMTDSequences. These graphs display delivery potential (Geometric Mean) ofaMTDs influenced with amino acid composition (A, I, V and L).

FIGS. 14a and 14b show Association of Cell-Permeability with CriticalFactors in aMTDs. These graphs show the association of cell-permeabilitywith critical factors [bending potential: proline position (PP),rigidity/flexibility: instability index (II), structural feature:aliphatic index (AI) and hydropathy: grand average of hydropathy(GRAVY)].

FIGS. 15a and 15b show Relative Relevance of aMTD-MediatedCell-Permeability with Critical Factors. Cell-permeability of 10 highand 10 low ranked aMTDs in their delivery potential were examined fortheir association with the critical factors [bending potential: prolineposition (PP), rigidity/flexibility: instability index (II), structuralfeature: aliphatic index (AI) and hydropathy: grand average ofhydropathy (GRAVY)].

In FIG. 15, aMTD888 has the sequence of SEQ ID NO: 222, aMTD825 has thesequence of SEQ ID NO: 188, aMTD895 has the sequence of SEQ ID NO: 226,aMTD896 has the sequence of SEQ ID NO: 227, aMTD727 has the sequence ofSEQ ID NO: 164, aMTD603 has the sequence of SEQ ID NO: 139, aMTD847 hasthe sequence of SEQ ID NO: 200, aMTD826 has the sequence of SEQ ID NO:189, aMTD724 has the sequence of SEQ ID NO: 161, aMTD563 has thesequence of SEQ ID NO: 131, aMTD43 has the sequence of SEQ ID NO: 15,aMTD103 has the sequence of SEQ ID NO: 29, aMTD4 has the sequence of SEQID NO:4, aMTD85 has the sequence of SEQ ID NO: 26, aMTD63 has thesequence of SEQ ID NO: 19, aMTD44 has the sequence of SEQ ID NO: 16,aMTD84 has the sequence of SEQ ID NO: 25, aMTD62 has the sequence of SEQID NO: 18, aMTD83 has the sequence of SEQ ID NO: 24, aMTD102 has thesequence of SEQ ID NO: 28.

FIG. 16 shows Relative Relevance of rPeptide-Mediated Cell-Permeabilitywith Hydropathy Range (GRAVY). This graph and a chart illustraterelative relevance of rPeptide-mediated cell-permeability with itshydropathy range (GRAVY).

In FIG. 16, rP33 has the sequence of SEQ ID NO: 927, rP931 has thesequence of SEQ ID NO: 882, rP436 has the sequence of SEQ ID NO: 932,rP138 has the sequence of SEQ ID NO: 933, rP77 has the sequence of SEQID NO: 934, rP936 has the sequence of SEQ ID NO: 883, rP226 has thesequence of SEQ ID NO: 892, rP30 has the sequence of SEQ ID NO: 926, rP6has the sequence of SEQ ID NO: 893, rP750 has the sequence of SEQ ID NO:894, rP67 has the sequence of SEQ ID NO: 906, rP20 has the sequence ofSEQ ID NO: 946, rP635 has the sequence of SEQ ID NO: 947, rP29 has thesequence of SEQ ID NO: 924, rP40 has the sequence of SEQ ID NO: 948,rP190 has the sequence of SEQ ID NO: 925, rP57 has the sequence of SEQID NO: 949, rP159 has the sequence of SEQ ID NO: 950, rP700 has thesequence of SEQ ID NO: 951. FIG. 17 shows a structure of iCP-SOCS3recombinant protein designed according to example 6-1.

In FIG. 17, aMTD165 has the sequence of SEQ ID NO: 43, SOCS3 has thesequence of SEQ ID NO: 814, SDA has the sequence of SEQ ID NO: 798, SDBhas the sequence of SEQ ID NO: 799, SDC has the sequence of SEQ ID NO:800, SDD has the sequence of SEQ ID NO: 801, SDF has the sequence of SEQID NO: 803, HS3 has SOCS3. SOCS3 has the sequence of SEQ ID NO: 814.HM₁₆₅S3 has aMTD165 and SOCS3. aMTD165 has the sequence of SEQ ID NO:43, SOCS3 has the sequence of SEQ ID NO: 814. HM₁₆₅S3A has aMTD165,SOCS3 and SDA. aMTD165 has the sequence of SEQ ID NO: 43, SOCS3 has thesequence of SEQ ID NO: 814, SDA has the sequence of SEQ ID NO: 798.HM₁₆₅S3B has the sequence of SEQ ID NO:827. HM₁₆₅S3C has aMTD165, SOCS3and SDC. aMTD165 has the sequence of SEQ ID NO: 43, SOCS3 has thesequence of SEQ ID NO: 814, SDC has the sequence of SEQ ID NO: 800.HM₁₆₅S3D has aMTD165, SOCS3 and SDD. aMTD165 has the sequence of SEQ IDNO: 43, SOCS3 has the sequence of SEQ ID NO: 814, SDD has the sequenceof SEQ ID NO: 801. HM₁₆₅S3E has aMTD165, SOCS3 and SDF. aMTD165 has thesequence of SEQ ID NO: 43, SOCS3 has the sequence of SEQ ID NO: 814, SDEhas the sequence of SEQ ID NO: 802.

FIG. 18 shows the agarose gel electrophoresis analysis showing plasmidDNA fragments insert encoding His-SOCS3-SDB (HS3B),His-aMTD165-SOCS3-SDB (HM165S3B), His-aMTD165-SOCS3-SDC (HM165S3C),His-aMTD165-SOCS3-SDD (HM165S3D), His-aMTD165-SOCS3-SDE (HM165S3E)cloned into the pET28a (+) vector according to example 6-1.

In FIG. 18 upper left panel, HS3B has SOCS3 and SDB. SOCS3 has thesequence of SEQ ID NO: 814, SDB has the sequence of SEQ ID NO: 799.HM₁₆₅S3 has aMTD165 and SOCS3. aMTD165 has the sequence of SEQ ID NO:43, SOCS3 has the sequence of SEQ ID NO: 814. HM₁₆₅S3B has the sequenceof SEQ ID NO:827. In FIG. 18 upper middle panel, HM₁₆₅S3A has aMTD165,SOCS3 and SDA. aMTD165 has the sequence of SEQ ID NO: 43, SOCS3 has thesequence of SEQ ID NO: 814, SDA has the sequence of SEQ ID NO: 798.HM₁₆₅S3B has the sequence of SEQ ID NO:827. In FIG. 18 upper rightpanel, HM₁₆₅S3B has the sequence of SEQ ID NO:827. In FIG. 18 lowerpanel, HM₁₆₅S3C has aMTD165, SOCS3 and SDC. aMTD165 has the sequence ofSEQ ID NO: 43, SOCS3 has the sequence of SEQ ID NO: 814, SDC has thesequence of SEQ ID NO: 800. HM₁₆₅S3D has aMTD165, SOCS3 and SDD. aMTD165has the sequence of SEQ ID NO: 43, SOCS3 has the sequence of SEQ ID NO:814, SDD has the sequence of SEQ ID NO: 801. HM₁₆₅S3E has aMTD165, SOCS3and SDF. aMTD165 has the sequence of SEQ ID NO: 43, SOCS3 has thesequence of SEQ ID NO: 814, SDE has the sequence of SEQ ID NO: 802.

FIG. 19 shows inducible expression and purification of iCP-SOCS3recombinant protein in E. coli according to example 6-2 and improvementof solubility/yield of iCP-SOCS3 recombinant protein by fusing aMTD/SDaccording to example 6-3.

In FIG. 19, HS3 has SOCS3. SOCS3 has the sequence of SEQ ID NO: 814.HM₁₆₅S3 has aMTD165 and SOCS3. aMTD165 has the sequence of SEQ ID NO:43, SOCS3 has the sequence of SEQ ID NO: 814. HM₁₆₅S3A has aMTD165,SOCS3 and SDA. aMTD165 has the sequence of SEQ ID NO: 43, SOCS3 has thesequence of SEQ ID NO: 814, SDA has the sequence of SEQ ID NO: 798.HM₁₆₅S3B has the sequence of SEQ ID NO:827. HM₁₆₅S3C has aMTD165, SOCS3and SDC. aMTD165 has the sequence of SEQ ID NO: 43, SOCS3 has thesequence of SEQ ID NO: 814, SDC has the sequence of SEQ ID NO: 800.HM₁₆₅S3D has aMTD165, SOCS3 and SDD. aMTD165 has the sequence of SEQ IDNO: 43, SOCS3 has the sequence of SEQ ID NO: 814, SDD has the sequenceof SEQ ID NO: 801. HM₁₆₅S3E has aMTD165, SOCS3 and SDF. aMTD165 has thesequence of SEQ ID NO: 43, SOCS3 has the sequence of SEQ ID NO: 814, SDEhas the sequence of SEQ ID NO: 802.

FIG. 20 shows aMTD-Mediated cell-permeability of SOCS3 recombinantproteins in RAW 264.7 cells according to example 7-1

In FIG. 20, HS3 has SOCS3. SOC3 has the sequence of SEQ ID NO:814.HM₁₆₅S3 has aMTD165 and SOCS3. aMTD165 has the sequence of SEQ ID NO:43,and SOC3 has the sequence of SEQ ID NO:814. HM₁₆₅S3A has aMTD165, SOCS3and SDA. aMTD165 has the sequence of SEQ ID NO:43, SOC3 has the sequenceof SEQ ID NO:814 and SDA has the sequence of SEQ ID NO:798. HM₁₆₅S3B hasthe sequence of SEQ ID NO:827. FIG. 21 shows aMTD-Mediated intracellulardelivery and localization of SOCS3 Recombinant Proteins in NIH3T3 cellscells according to example 7-1.

In FIG. 21, HS3 has SOCS3. SOC3 has the sequence of SEQ ID NO:814.HM₁₆₅S3 has aMTD165 and SOCS3. aMTD165 has the sequence of SEQ ID NO:43,and SOC3 has the sequence of SEQ ID NO:814. HM₁₆₅S3A has aMTD165, SOCS3and SDA. aMTD165 has the sequence of SEQ ID NO:43, SOC3 has the sequenceof SEQ ID NO:814 and SDA has the sequence of SEQ ID NO:798. HM₁₆₅S3B hasthe sequence of SEQ ID NO:827. FIG. 22 shows systemic delivery ofaMTD/SD-fused SOCS3 recombinant proteins in vivo according to example7-2.

In FIG. 22, HS3 has SOCS3. SOC3 has the sequence of SEQ ID NO:814.HM₁₆₅S3 has aMTD165 and SOCS3. aMTD165 has the sequence of SEQ ID NO:43,and SOC3 has the sequence of SEQ ID NO:814. HM₁₆₅S3A has aMTD165, SOCS3and SDA. aMTD165 has the sequence of SEQ ID NO:43, SOC3 has the sequenceof SEQ ID NO:814 and SDA has the sequence of SEQ ID NO:798. HM₁₆₅S3B hasthe sequence of SEQ ID NO:827. FIG. 23 shows inhibition of IFN-γ-inducedSTAT phosphorylation by iCP-SOCS3 recombinant protein according toexample 8-1.

In FIG. 23, HS3 has SOCS3. SOC3 has the sequence of SEQ ID NO:814.HM₁₆₅S3 has aMTD165 and SOCS3. aMTD165 has the sequence of SEQ ID NO:43,and SOC3 has the sequence of SEQ ID NO:814. HM₁₆₅S3A has aMTD165, SOCS3and SDA. aMTD165 has the sequence of SEQ ID NO:43, SOC3 has the sequenceof SEQ ID NO:814 and SDA has the sequence of SEQ ID NO:798. HM₁₆₅S3B hasthe sequence of SEQ ID NO:827. FIG. 24 shows inhibition of LPS-inducedcytokines secretion by iCP-SOCS3 recombinant protein according toexample 8-2.

In FIG. 24, HS3 has SOCS3. SOC3 has the sequence of SEQ ID NO:814.HM₁₆₅S3 has aMTD165 and SOCS3. aMTD165 has the sequence of SEQ ID NO:43,and SOC3 has the sequence of SEQ ID NO:814. HM₁₆₅S3A has aMTD165, SOCS3and SDA. aMTD165 has the sequence of SEQ ID NO:43, SOC3 has the sequenceof SEQ ID NO:814 and SDA has the sequence of SEQ ID NO:798. HM₁₆₅S3B hasthe sequence of SEQ ID NO:827. FIG. 25 shows the structures of SOCS3recombinant protein lacking aMTD prepared as a negative controlaccording to example 9.

In FIG. 25, SOSCS3 has the sequence of SEQ ID NO:814, and SDB has thesequence of SEQ ID NO:799. HS3B has SOCS3 and SDB. SOC3 has the sequenceof SEQ ID NO:814 and SDB has the sequence of SEQ ID NO: 799.

FIG. 26 shows expression, purification, and solubility/yield of HS3(lacking aMTD and SD) and HS3B (lacking aMTD) determined according toexample 6-3.

In FIG. 26, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HS3 has SOCS3. SOC3has the sequence of SEQ ID NO:814.

FIG. 27 shows the agarose gel electrophoresis analysis showing plasmidDNA fragments insert encoding His-aMTD #-SOCS3-SDB (HM # S3B) and His-rP#-SOCS3-SDB cloned into the pET28a (+) vector according to example 10.

In FIG. 27, aMTD44 has the sequence of SEQ ID NO:16, aMTD81 has thesequence of SEQ ID NO:22, aMTD123 has the sequence of SEQ ID NO:33,aMTD162 has the sequence of SEQ ID NO:40, aMTD281 has the sequence ofSEQ ID NO:65, aMTD324 has the sequence of SEQ ID NO:77, aMTD364 has thesequence of SEQ ID NO:85, aMTD365 has the sequence of SEQ ID NO:86,aMTD622 has the sequence of SEQ ID NO:142, aMTD662 has the sequence ofSEQ ID NO:822, aMTD563 has the sequence of SEQ ID NO:131, aMTD899 hasthe sequence of SEQ ID NO:223, aMTD897 has the sequence of SEQ IDNO:228, aMTD623 has the sequence of SEQ ID NO:143, aMTD908 has thesequence of SEQ ID NO:237, aMTD911 has the sequence of SEQ ID NO:239,aMTD904 has the sequence of SEQ ID NO:233, aMTD481 has the sequence ofSEQ ID NO:110, aMTD787 has the sequence of SEQ ID NO:177, aMTD363 hasthe sequence of SEQ ID NO:84, aMTD121 has the sequence of SEQ ID NO:32,rP921 has the sequence of SEQ ID NO:931, rP16 has the sequence of SEQ IDNO:890, rP67 has the sequence of SEQ ID NO:906, rP29 has the sequence ofSEQ ID NO:924, and rP700 has the sequence of SEQ ID NO:951.

FIGS. 28a and 28b show expression, purification, and solubility/yield ofHis-aMTD #-SOCS3-SDB (HM # S3B) determined according to example 10.

In FIG. 28a , aMTD44 has the sequence of SEQ ID NO:16, aMTD81 has thesequence of SEQ ID NO:22, aMTD281 has the sequence of SEQ ID NO:65,aMTD324 has the sequence of SEQ ID NO:77, aMTD364 has the sequence ofSEQ ID NO:85, and aMTD365 has the sequence of SEQ ID NO:86.

In FIG. 28b , aMTD563 has the sequence of SEQ ID NO:131, aMTD662 has thesequence of SEQ ID NO:822, aMTD622 has the sequence of SEQ ID NO:142,aMTD904 has the sequence of SEQ ID NO:233, aMTD908 has the sequence ofSEQ ID NO:237, aMTD911 has the sequence of SEQ ID NO:239, aMTD897 hasthe sequence of SEQ ID NO:228, aMTD899 has the sequence of SEQ IDNO:223, and aMTD623 has the sequence of SEQ ID NO:143.

FIG. 29 shows expression, purification, and solubility/yield of His-rP#-SOCS3-SDB (HrP # S3B) determined according to example 10.

In FIG. 29, rP700 has the sequence of SEQ ID NO:951, and rP921 has thesequence of SEQ ID NO:931.

FIG. 30 shows solubility/yield of His-aMTD #-SOCS3-SDB (HM # S3B)determined according to example 10.

In FIG. 30, aMTD121 has the sequence of SEQ ID NO:32, aMTD623 has thesequence of SEQ ID NO:143, aMTD165 has the sequence of SEQ ID NO:43,aMTD81 has the sequence of SEQ ID NO:22, aMTD162 has the sequence of SEQID NO:40, aMTD365 has the sequence of SEQ ID NO:86, aMTD481 has thesequence of SEQ ID NO:110, aMTD2 has the sequence of SEQ ID NO:2,aMTD281 has the sequence of SEQ ID NO:65, aMTD324 has the sequence ofSEQ ID NO:77, aMTD908 has the sequence of SEQ ID NO:237, aMTD363 has thesequence of SEQ ID NO:84, aMTD911 has the sequence of SEQ ID NO:239,aMTD264 has the sequence of SEQ ID NO:63, aMTD662 has the sequence ofSEQ ID NO:142, aMTD563 has the sequence of SEQ ID NO:131, aMTD662 hasthe sequence of SEQ ID NO:822, aMTD897 has the sequence of SEQ IDNO:228, aMTD44 has the sequence of SEQ ID NO:16, aMTD899 has thesequence of SEQ ID NO:223, aMTD364 has the sequence of SEQ ID NO:85, andaMTD904 has the sequence of SEQ ID NO:233.

FIG. 31 shows aMTD-mediated cell-permeability. The cell-permeability ofeach SOCS3 recombinant protein fused with SD and various aMTD isvisually compared to that of the cargo protein lacking CPP (HS3B) orlacking CPP and SD (HS3). Gray shaded area represents untreated E. colicells (diluent); green line represents the cells treated with equalmolar concentration of FITC (FITC only); black line indicates the cellstreated with FITC-his-SOCS protein (FITC-HS3); blue line indicates thecells treated with FITC-his-SOCS-SDB protein (FITC-HS3B) purple lineindicates the cells treated with FITC-his-aMTD #-SOCS-SDB protein(FITC-HM # S3B).

In FIG. 31, HS3 has SOCS3. SOC3 has the sequence of SEQ ID NO:814. HS3Bhas SOCS3 and SDB. SOC3 has the sequence of SEQ ID NO:814 and SDB hasthe sequence of SEQ ID NO: 799. HM₁₆₅S3B has the sequence of SEQ IDNO:827. aMTD2 has the sequence of SEQ ID NO:2, aMTD44 has the sequenceof SEQ ID NO:16, aMTD81 has the sequence of SEQ ID NO:22, aMTD121 hasthe sequence of SEQ ID NO:32, aMTD162 has the sequence of SEQ ID NO:40,aMTD284 has the sequence of SEQ ID NO:68, aMTD281 has the sequence ofSEQ ID NO:65, aMTD324 has the sequence of SEQ ID NO:77, aMTD363 has thesequence of SEQ ID NO:84, aMTD364 has the sequence of SEQ ID NO:85,aMTD365 has the sequence of SEQ ID NO:86, aMTD481 has the sequence ofSEQ ID NO:110, aMTD563 has the sequence of SEQ ID NO:131, aMTD622 hasthe sequence of SEQ ID NO:142, aMTD623 has the sequence of SEQ IDNO:143, aMTD662 has the sequence of SEQ ID NO:822, aMTD897 has thesequence of SEQ ID NO:228, aMTD889 has the sequence of SEQ ID NO:223,aMTD904 has the sequence of SEQ ID NO:233, aMTD908 has the sequence ofSEQ ID NO:237, and aMTD911 has the sequence of SEQ ID NO:239.

FIG. 32 shows relative cell-permeability of His-aMTD #-SOCS3-SDB-Fusedrecombinant proteins Compared to control (Vehicle, FITC only, HS3 andHS3B).

In FIG. 32, HS3 has SOCS3. SOC3 has the sequence of SEQ ID NO:814. HS3Bhas SOCS3 and SDB. SOC3 has the sequence of SEQ ID NO:814 and SDB hasthe sequence of SEQ ID NO: 799. aMTD121 has the sequence of SEQ IDNO:32, aMTD623 has the sequence of SEQ ID NO:143, aMTD165 has thesequence of SEQ ID NO:43, aMTD81 has the sequence of SEQ ID NO:22,aMTD162 has the sequence of SEQ ID NO:40, aMTD365 has the sequence ofSEQ ID NO:86, aMTD481 has the sequence of SEQ ID NO:110, aMTD2 has thesequence of SEQ ID NO:2, aMTD281 has the sequence of SEQ ID NO:65,aMTD324 has the sequence of SEQ ID NO:77, aMTD908 has the sequence ofSEQ ID NO:237, aMTD363 has the sequence of SEQ ID NO:84, aMTD911 has thesequence of SEQ ID NO:239, aMTD264 has the sequence of SEQ ID NO:63,aMTD662 has the sequence of SEQ ID NO:822, aMTD563 has the sequence ofSEQ ID NO:131, aMTD622 has the sequence of SEQ ID NO:142, aMTD897 hasthe sequence of SEQ ID NO:228, aMTD44 has the sequence of SEQ ID NO:16,aMTD899 has the sequence of SEQ ID NO:223, aMTD364 has the sequence ofSEQ ID NO:85, and aMTD904 has the sequence of SEQ ID NO:233.

FIG. 33 shows random Peptide-Mediated cell-permeability. Thecell-permeability of each SOCS3 recombinant protein fused with SDB andaMTD165 or various rP is visually compared to that of the cargo proteinlacking CPP (HS3B) or lacking CPP and SD (HS3). Gray shaded arearepresents untreated E. coli cells (diluent); green line represents thecells treated with equal molar concentration of FITC (FITC only); blackline indicates the cells treated with FITC-his-SOCS protein (FITC-H53);blue line indicates the cells treated with FITC-his-SOCS-SDB protein(FITC-HS3B) and purple line indicates the cells treated withFITC-his-rPeptide #-SOCS-SDB protein (FITC-HrP # S3B).

In FIG. 33, HS3 has SOCS3. SOC3 has the sequence of SEQ ID NO:814. HS3Bhas SOCS3 and SDB. SOC3 has the sequence of SEQ ID NO:814 and SDB hasthe sequence of SEQ ID NO: 799. HM₁₆₅S3B has the sequence of SEQ IDNO:827. rP16 has the sequence of SEQ ID NO:890, rP29 has the sequence ofSEQ ID NO:924, rP67 has the sequence of SEQ ID NO:906, rP700 has thesequence of SEQ ID NO:951, and rP921 has the sequence of SEQ ID NO:931.

FIG. 34 shows relative cell-permeability of His-rP #-SOCS3-SDB-Fusedrecombinant proteins Compared to control (Vehicle, FITC only, HS3 andHS3B).

In FIG. 34, HS3 has SOCS3. SOC3 has the sequence of SEQ ID NO:814. HS3Bhas SOCS3 and SDB. SOC3 has the sequence of SEQ ID NO:814 and SDB hasthe sequence of SEQ ID NO: 799. rP921 has the sequence of SEQ ID NO:931,rP700 has the sequence of SEQ ID NO:951, rP16 has the sequence of SEQ IDNO:890, rP67 has the sequence of SEQ ID NO:906, and rP29 has thesequence of SEQ ID NO:924.

FIG. 35 shows apoptotic cells analysis according to example 11-1.

In FIG. 35, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has aMTD165,SOCS3 and SDB. aMTD165 has the sequence of SEQ ID NO:43, SOC3 has thesequence of SEQ ID NO:814 and SDB has the sequence of SEQ ID NO: 799.HM₂₈₁S3B has aMTD281, SOCS3 and SDB. aMTD281 has the sequence of SEQ IDNO:65, SOC3 has the sequence of SEQ ID NO:814 and SDB has the sequenceof SEQ ID NO: 799. HM₃₂₄S3B has aMTD324, SOCS3 and SDB. aMTD324 has thesequence of SEQ ID NO:77, SOC3 has the sequence of SEQ ID NO:814 and SDBhas the sequence of SEQ ID NO: 799. HM₅₆₃S3B has aMTD563, SOCS3 and SDB.aMTD563 has the sequence of SEQ ID NO:131, SOC3 has the sequence of SEQID NO:814 and SDB has the sequence of SEQ ID NO: 799. HM₉₀₄S3B hasaMTD904, SOCS3 and SDB. aMTD904 has the sequence of SEQ ID NO:233, SOC3has the sequence of SEQ ID NO:814 and SDB has the sequence of SEQ ID NO:799.

FIG. 36 shows induction of apoptosis by iCP-SOCS3 recombinant proteinsaccording to example 11-2.

In FIG. 36, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has aMTD165,SOCS3 and SDB. aMTD165 has the sequence of SEQ ID NO:43, SOC3 has thesequence of SEQ ID NO:814 and SDB has the sequence of SEQ ID NO: 799.HM₂₈₁S3B has aMTD281, SOCS3 and SDB. aMTD281 has the sequence of SEQ IDNO:65, SOC3 has the sequence of SEQ ID NO:814 and SDB has the sequenceof SEQ ID NO: 799. HM₃₂₄S3B has aMTD324, SOCS3 and SDB. aMTD324 has thesequence of SEQ ID NO:77, SOC3 has the sequence of SEQ ID NO:814 and SDBhas the sequence of SEQ ID NO: 799. HM₅₆₃S3B has aMTD563, SOCS3 and SDB.aMTD563 has the sequence of SEQ ID NO:131, SOC3 has the sequence of SEQID NO:814 and SDB has the sequence of SEQ ID NO: 799. HM₉₀₄S3B hasaMTD904, SOCS3 and SDB. aMTD904 has the sequence of SEQ ID NO:233, SOC3has the sequence of SEQ ID NO:814 and SDB has the sequence of SEQ ID NO:799.

FIG. 37 shows cell migration inhibition by iCP-SOCS3 recombinant proteinaccording to example 11-3.

In FIG. 37, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has thesequence of SEQ ID NO:827. HM₂₈₁S3B has aMTD281, SOCS3 and SDB. aMTD281has the sequence of SEQ ID NO:65, SOC3 has the sequence of SEQ ID NO:814and SDB has the sequence of SEQ ID NO: 799. HM₃₂₄S3B has aMTD324, SOCS3and SDB. aMTD324 has the sequence of SEQ ID NO:77, SOC3 has the sequenceof SEQ ID NO:814 and SDB has the sequence of SEQ ID NO: 799. HM₅₆₃S3Bhas aMTD563, SOCS3 and SDB. aMTD563 has the sequence of SEQ ID NO:131,SOC3 has the sequence of SEQ ID NO:814 and SDB has the sequence of SEQID NO: 799. HM₉₀₄S3B has aMTD904, SOCS3 and SDB. aMTD904 has thesequence of SEQ ID NO:233, SOC3 has the sequence of SEQ ID NO:814 andSDB has the sequence of SEQ ID NO: 799.

FIG. 38 shows solubility/yield, permeability and biological activity ofHis-aMTD #-SOCS3-SDB (HM # S3B) determined according to example 10 to11-3.

In FIG. 38, aMTD481 has the sequence of SEQ ID NO:110, aMTD364 has thesequence of SEQ ID NO:85, aMTD363 has the sequence of SEQ ID NO:84,aMTD324 has the sequence of SEQ ID NO:77, aMTD281 has the sequence ofSEQ ID NO:65, aMTD2 has the sequence of SEQ ID NO:2, aMTD563 has thesequence of SEQ ID NO:131, aMTD165 has the sequence of SEQ ID NO:43,aMTD897 has the sequence of SEQ ID NO:228, aMTD622 has the sequence ofSEQ ID NO:822, aMTD44 has the sequence of SEQ ID NO:16, aMTD121 has thesequence of SEQ ID NO:32, aMTD623 has the sequence of SEQ ID NO:143,aMTD81 has the sequence of SEQ ID NO:22, aMTD162 has the sequence of SEQID NO:40, and aMTD365 has the sequence of SEQ ID NO:86.

FIG. 39 shows expression, purification, and solubility/yield of M165S3SB(lacking his-tag) determined according to example 12-1.

In FIG. 39, aMTD165 has the sequence of SEQ ID NO:43, SOCS3 has thesequence of SEQ ID NO:814, SDB has the sequence of SEQ ID NO:799, andM₁₆₅S3B has aMTD165, SOCS3 and SDB. aMTD165 has the sequence of SEQ IDNO:43, SOCS3 has the sequence of SEQ ID NO:814, and SDB has the sequenceof SEQ ID NO: 799.

FIG. 40 shows cell-permeability of SOCS3 recombinant proteins (lackinghis-tag) in RAW 264.7 cells according to example 12-2.

In FIG. 40, HM₁₆₅S3B has the sequence of SEQ ID NO: 827, and M₁₆₅S3B hasaMTD165, SOCS3 and SDB. aMTD165 has the sequence of SEQ ID NO:43, SOCS3has the sequence of SEQ ID NO:814, and SDB has the sequence of SEQ IDNO: 799.

FIG. 41 shows Annexin V analysis according to example 12-3.

In FIG. 41, HM₁₆₅S3B has the sequence of SEQ ID NO: 827, and M₁₆₅S3B hasaMTD165, SOCS3 and SDB. aMTD165 has the sequence of SEQ ID NO:43, SOCS3has the sequence of SEQ ID NO:814, and SDB has the sequence of SEQ IDNO: 799.

FIG. 42 shows cell migration inhibition (bottom) by iCP-SOCS3recombinant protein according to example 12-3.

In FIG. 42, HM₁₆₅S3B has the sequence of SEQ ID NO: 827, and M₁₆₅S3B hasaMTD165, SOCS3 and SDB. aMTD165 has the sequence of SEQ ID NO:43, SOCS3has the sequence of SEQ ID NO:814, and SDB has the sequence of SEQ IDNO: 799.

FIG. 43 shows a structure of iCP-SOCS3 recombinant protein(His-aMTD165-SOCS3-SDB′) constructed according to example 12-4.

In FIG. 43, HM₁₆₅S3B has the sequence of SEQ ID NO:827. HM₁₆₅S3B (V28L)has aMTD165, SOCS3 and SDB′. aMTD165 has the sequence of SEQ ID NO:43,SOCS3 has the sequence of SEQ ID NO:814, and SDB′ has the sequence ofSEQ ID NO:804.

FIG. 44 shows expression, purification, and solubility/yield ofHM165S3SB (SEQ ID NO:827) and HM165S3SB′ determined according to example12-4.

In FIG. 44, In FIG. 43, HM₁₆₅S3B has the sequence of SEQ ID NO:827.HM₁₆₅S3B (V28L) has aMTD165, SOCS3 and SDB′. aMTD165 has the sequence ofSEQ ID NO:43, SOCS3 has the sequence of SEQ ID NO:814, and SDB′ has thesequence of SEQ ID NO:804.

FIG. 45 shows aMTD-Mediated cell-permeability of iCP-SOCS3 recombinantproteins (HM165S3B (SEQ ID NO:827) and HM165S3B′(V28L)) in RAW 264.7cells according to example 12-5.

In FIG. 45, In FIG. 43, HM₁₆₅S3B has the sequence of SEQ ID NO:827.HM₁₆₅S3B (V28L) has aMTD165, SOCS3 and SDB′. aMTD165 has the sequence ofSEQ ID NO:43, SOCS3 has the sequence of SEQ ID NO:814, and SDB′ has thesequence of SEQ ID NO:804.

FIG. 46 shows antiproliferative activity of iCP-SOCS3 recombinantproteins (HM165S3B (SEQ ID NO:827) and HM165S3B′(V28L)) according toexample 12-6.

In FIG. 46, HM₁₆₅S3B has the sequence of SEQ ID NO:827. HM₁₆₅S3B (V28L)has aMTD165, SOCS3 and SDB′. aMTD165 has the sequence of SEQ ID NO:43,SOCS3 has the sequence of SEQ ID NO:814, and SDB′ has the sequence ofSEQ ID NO:804.

FIG. 47 shows induction of apoptosis by iCP-SOCS3 recombinant proteins(HM165S3B (SEQ ID NO:827) and HM165S3B′(V28L)) according to example12-6.

In FIG. 47, HM₁₆₅S3B has the sequence of SEQ ID NO:827. HM₁₆₅S3B (V28L)has aMTD165, SOCS3 and SDB′. aMTD165 has the sequence of SEQ ID NO:43,SOCS3 has the sequence of SEQ ID NO:814, and SDB′ has the sequence ofSEQ ID NO:804.

FIG. 48 shows cell migration inhibition by SOCS3 recombinant proteins(HM165S3B (SEQ ID NO:827) and HM165S3B′(V28L)) according to example12-6.

In FIG. 48, HM₁₆₅S3B has the sequence of SEQ ID NO:827. HM₁₆₅S3B (V28L)has aMTD165, SOCS3 and SDB′. aMTD165 has the sequence of SEQ ID NO:43,SOCS3 has the sequence of SEQ ID NO:814, and SDB′ has the sequence ofSEQ ID NO:804.

FIG. 49a shows a structure of iCP-SOCS3 recombinant proteins (top) andagarose gel electrophoresis analysis (bottom) according to example 12-7and FIG. 49b shows inducible expressions and purifications of iCP-SOCS3recombinant protein in E. coli (bottom) according to example 12-7.

In FIG. 49a , aMTD165 has the sequence of SEQ ID NO:43, SOCS3 has thesequence of SEQ ID NO:814, SDB has the sequence of SEQ ID NO:799.BS3M₁₆₅ has SDB, SOCS3 and aMTD165. SDB has the sequence of SEQ ID NO:799, SOC3 has the sequence of SEQ ID NO:814 and aMTD165 has the sequenceof SEQ ID NO:43. (V28L)BS3M165 has SDB′, SOCS3 and aTMD165. SDB′ has thesequence of SEQ ID NO:804, SOCS3 has the sequence of SEQ ID NO:814,aMTD165 has the sequence of SEQ ID NO:43. In FIG. 49b , BS3M₁₆₅ has SDB,SOCS3 and aMTD165. SDB has the sequence of SEQ ID NO: 799, SOC3 has thesequence of SEQ ID NO:814 and aMTD165 has the sequence of SEQ ID NO:43.(V28L)BS3M165 has SDB′, SOCS3 and aMTD165. SDB′ has the sequence of SEQID NO:804, SOCS3 has the sequence of SEQ ID NO:814, aMTD165 has thesequence of SEQ ID NO:43.

FIG. 50 shows inhibition of IFN-γ-induced STAT phosphorylation byiCP-SOCS3 recombinant protein according to example 13.

In FIG. 50, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has thesequence of SEQ ID NO:827.

FIG. 51 shows effect of treating EDTA (FIG. 53A) and proteinase K (FIG.53B) on aMTD-mediated SOCS3 protein uptake into cells according toexample 14.

In FIG. 51, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has thesequence of SEQ ID NO:827.

FIG. 52 shows effect of treating taxol (FIG. 52A) and antimycin (FIG.52B) on aMTD-mediated SOCS3 protein uptake into cells according toexample 14.

In FIG. 52, HS3 has SOCS3. SOC3 has the sequence of SEQ ID NO:814. HS3Bhas SOCS3 and SDB. SOC3 has the sequence of SEQ ID NO:814 and SDB hasthe sequence of SEQ ID NO: 799. HM₁₆₅S3B has the sequence of SEQ IDNO:827.

FIG. 53 shows effect of temperature on aMTD-mediated SOCS3 proteinuptake into cells according to example 14.

In FIG. 53, HS3 has SOCS3. SOC3 has the sequence of SEQ ID NO:814. HS3Bhas SOCS3 and SDB. SOC3 has the sequence of SEQ ID NO:814 and SDB hasthe sequence of SEQ ID NO: 799. HM₁₆₅S3B has the sequence of SEQ IDNO:827.

FIG. 54 shows aMTD-mediated cell-to-cell delivery assessed according toexample 14.

In FIG. 54, HS3 has SOCS3. SOC3 has the sequence of SEQ ID NO:814. HS3Bhas SOCS3 and SDB. SOC3 has the sequence of SEQ ID NO:814 and SDB hasthe sequence of SEQ ID NO: 799. HM₁₆₅S3B has the sequence of SEQ IDNO:827.

FIG. 55 shows bioavailability of iCP-SOCS3 recombinant protein in PBMC,splenocytes and hepatocytes analyzed by fluorescence microscopyaccording to example 15-2.

FIG. 56 shows biodistribution of iCP-SOCS3 recombinant protein inpancreas tissues analyzed by confocal microscope according to example15-3.

FIG. 57 shows aMTD-Mediated cell-permeability of SOCS3 recombinantproteins in PANC-1 cells according to example 16-2.

In FIG. 57, HS3 has SOCS3. SOC3 has the sequence of SEQ ID NO:814. HS3Bhas SOCS3 and SDB. SOC3 has the sequence of SEQ ID NO:814 and SDB hasthe sequence of SEQ ID NO: 799. HM₁₆₅S3B has the sequence of SEQ IDNO:827.

FIG. 58 shows antiproliferative activity of iCP-SOCS3 recombinantprotein according to example 17.

In FIG. 58, HS3 has SOCS3. SOC3 has the sequence of SEQ ID NO:814.HM₁₆₅S3 has aMTD165 and SOCS3. aMTD165 has the sequence of SEQ ID NO:43,SOC3 has the sequence of SEQ ID NO:814. HM₁₆₅S3A has aMTD165, SOCS3 andSDA. aMTD165 has the sequence of SEQ ID NO:43, SOC3 has the sequence ofSEQ ID NO:814 and SDA has the sequence of SEQ ID NO:798. HM₁₆₅S3B hasthe sequence of SEQ ID NO:827.

FIGS. 59a and 59b show cell migration inhibition activity by iCP-SOCS3recombinant protein according to example 18.

In FIG. 59a , HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799, and HM₁₆₅S3B has thesequence of SEQ ID NO:827.

FIG. 60 shows transwell migration inhibition activity by iCP-SOCS3recombinant protein in PANC-1 cells according to example 18.

In FIG. 60, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799, and HM₁₆₅S3B has thesequence of SEQ ID NO:827.

FIG. 61 shows decrease in invasion by iCP-SOCS3 recombinant protein inPANC-1 cells analyzed according to example 18.

In FIG. 61, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799, and HM₁₆₅S3B has thesequence of SEQ ID NO:827.

FIG. 62 shows induction of apoptosis of pancreatic cancer cells byiCP-SOCS3 recombinant proteins assessed by Annexin V staining accordingto example 19-1.

FIGS. 63a and 63b show induction of apoptosis in normal cells (FIG. 63a) and in PANC-1 (FIG. 63b ) by iCP-SOCS3 recombinant proteins assessedby by flow cytometric analysis according to example 19-2.

FIG. 64 shows induction of apoptosis in PANC-1 cells by iCP-SOCS3recombinant proteins analyzed by TUNEL assay according to example 19-3.

In FIG. 64, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799, and HM₁₆₅S3B has thesequence of SEQ ID NO:827.

FIG. 65 shows down regulation of expression of apoptosis by iCP-SOCS3recombinant proteins in PANC-1 determined by immunoblotting according toexample 20.

In FIG. 65, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799, and HM₁₆₅S3B has thesequence of SEQ ID NO:827. FIGS. 66a and 66b show suppression of thetumor growth by iCP-SOCS3 recombinant proteins in PANC-1 assessedaccording to example 21.

In FIG. 66a , HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799, and HM₁₆₅S3B has thesequence of SEQ ID NO:827. In FIG. 66b , HS3B has SOCS3 and SDB. SOC3has the sequence of SEQ ID NO:814 and SDB has the sequence of SEQ ID NO:799, and HM₁₆₅S3B has the sequence of SEQ ID NO:827.

FIG. 67 shows change of biomarker expression by iCP-SOCS3 recombinantproteins in PANC-1-derived xenograft model determined by RT-PCRaccording to example 21.

In FIG. 67, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799, and HM₁₆₅S3B has thesequence of SEQ ID NO:827.

FIG. 68 shows change of biomarker expression by iCP-SOCS3 recombinantproteins in PANC-1-derived xenograft model determined by immunoblottingaccording to example 21.

In FIG. 68, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799, and HM₁₆₅S3B has thesequence of SEQ ID NO:827.

FIG. 69 shows change of biomarker expression by iCP-SOCS3 recombinantproteins in PANC-1-derived xenograft model determined by immunoblottingaccording to example 21.

In FIG. 69, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799, and HM₁₆₅S3B has thesequence of SEQ ID NO:827.

FIG. 70 shows humanized SDB domain according to example 12-4.

In FIG. 70, Peptide W27 has the sequence of SEQ ID NO:1010, and PeptideV28 has the sequence of SEQ ID NO:1011.

FIG. 71 shows sequences of amino acid and nucleotide of basic CPP, andprimers used in example 6-4.

In FIG. 71, SOS3 has the sequence of SEQ ID NO:814. TAT has the sequenceof SEQ ID NO:382. Poly R has the sequence of SEQ ID NO:834.

FIG. 72 shows structure, expression, purification and solubility/yieldof aMTD/SD-fused SOCS3 recombinant protein and basic CPP/SD-fused SOCS3recombinant protein analyzed according to example 6-4.

In FIG. 72, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has thesequence of SEQ ID NO:827. HTS3B has TAT, SOCS3 and SDB. TAT has thesequence of SEQ ID NO:382, SOC3 has the sequence of SEQ ID NO:814 andSDB has the sequence of SEQ ID NO: 799. HRS3B has Poly R, SOCS3 and SDB.Poly R has the sequence of SEQ ID NO:834, SOC3 has the sequence of SEQID NO:814 and SDB has the sequence of SEQ ID NO: 799.

FIG. 73 shows comparison of cell-permeability between aMTD/SD fusedSOCS3 recombinant proteins and basic CPP/SD-fused in RAW 264.7 cellsaccording to example 7-1-2.

In FIG. 73, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has thesequence of SEQ ID NO:827. HTS3B has TAT, SOCS3 and SDB. TAT has thesequence of SEQ ID NO:382, SOC3 has the sequence of SEQ ID NO:814 andSDB has the sequence of SEQ ID NO: 799. HRS3B has Poly R, SOCS3 and SDB.Poly R has the sequence of SEQ ID NO:834, SOC3 has the sequence of SEQID NO:814 and SDB has the sequence of SEQ ID NO: 799.

FIG. 74 shows comparison of tissue-permeability between aMTD/SD fusedSOCS3 recombinant proteins and basic CPP/SD-fused in various tissues ofICR mice according to example 7-2-2.

In FIG. 74, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has thesequence of SEQ ID NO:827. HTS3B has TAT, SOCS3 and SDB. TAT has thesequence of SEQ ID NO:382, SOC3 has the sequence of SEQ ID NO:814 andSDB has the sequence of SEQ ID NO: 799. HRS3B has Poly R, SOCS3 and SDB.Poly R has the sequence of SEQ ID NO:834, SOC3 has the sequence of SEQID NO:814 and SDB has the sequence of SEQ ID NO: 799.

FIG. 75 shows effect of treating proteinase K (A) and Taxol (B) on aMTD(or basic CPP)-mediated SOCS3 protein uptake into cells according toexample 14-2.

In FIG. 75, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has thesequence of SEQ ID NO:827. HTS3B has TAT, SOCS3 and SDB. TAT has thesequence of SEQ ID NO:382, SOC3 has the sequence of SEQ ID NO:814 andSDB has the sequence of SEQ ID NO: 799. HRS3B has Poly R, SOCS3 and SDB.Poly R has the sequence of SEQ ID NO:834, SOC3 has the sequence of SEQID NO:814 and SDB has the sequence of SEQ ID NO: 799.

FIGS. 76 and 77 show aMTD (or basic CPP)-mediated cell-to-cell delivery(FIG. 76) and cell-to-cell function (FIG. 77) assessed according toexample 14-2.

In FIG. 76, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has thesequence of SEQ ID NO:827. HTS3B has TAT, SOCS3 and SDB. TAT has thesequence of SEQ ID NO:382, SOC3 has the sequence of SEQ ID NO:814 andSDB has the sequence of SEQ ID NO: 799. HRS3B has Poly R, SOCS3 and SDB.Poly R has the sequence of SEQ ID NO:834, SOC3 has the sequence of SEQID NO:814 and SDB has the sequence of SEQ ID NO: 799. In FIG. 77,HM₁₆₅S3B has the sequence of SEQ ID NO:827. HTS3B has TAT, SOCS3 andSDB. TAT has the sequence of SEQ ID NO:382, SOC3 has the sequence of SEQID NO:814 and SDB has the sequence of SEQ ID NO: 799.

FIGS. 78a and 78b show dose-dependency of cell-permeability of iCP-SOCS3recombinant proteins analyzed according to example 15-1.

In FIG. 78a , HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has thesequence of SEQ ID NO:827. In FIG. 78b , HM₁₆₅S3B has the sequence ofSEQ ID NO:827. FIG. 79 shows time-dependency of cell-permeability of theiCP-SOCS3 recombinant proteins analyzed according to example 15-1.

In FIG. 79, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has thesequence of SEQ ID NO:827. FIG. 80 shows the established pancreaticcancer xenograft model (A) and tumor targeting of the iCP-SOCS3recombinant proteins (B) assessed according to example 16-1.

FIG. 81 shows methylation and unmethylation level of endogenous SOCS3 incell line analyzed by the agarose gel electrophoresis according toexample 16-3-1.

FIG. 82 shows expression level of endogenous SOCS3 mRNA in pancreaticcancer cell line analyzed according to example 16-3-2.

FIG. 83 shows expression level of SOCS3 gene, and phosphorylation ofJAK1 and JAK2 in cell line analyzed by the agarose gel electrophoresisaccording to example 16-3-3.

FIG. 84 shows regulation of expression of biomarker by iCP-SOCS3recombinant proteins in BxPC-3 cells analyzed by immunoblottingaccording to example 20.

In FIG. 84, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has thesequence of SEQ ID NO:827.

FIG. 85 shows change of biomarker expression by iCP-SOCS3 recombinantproteins in PANC-1-derived xenograft model determined by immunoblottingaccording to example 21.

In FIG. 85, HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has thesequence of SEQ ID NO:827. FIG. 86a shows the four groups of thepancreatic cancer xenograft model according to example 22.

FIG. 86b shows four IV injection groups (Diluent, 50 mg/kg ofGemcitabine (Gemzar®), 3 mg/kg of iCP-SOCS3, and 30 mg/kg of iCP-SOCS3)(A) and anticancer efficacy of iCP-SOCS3 recombinant proteins analyzedby western blot analysis (B) according to example 22.

FIGS. 87a and 87b show dose-dependent inhibition of tumor growth byiCP-SOCS3 recombinant protein according to example 23.

In FIG. 87a, b HS3B has SOCS3 and SDB. SOC3 has the sequence of SEQ IDNO:814 and SDB has the sequence of SEQ ID NO: 799. HM₁₆₅S3B has thesequence of SEQ ID NO:827.

MODE FOR INVENTION

1. Analysis of Reference Hydrophobic CPPs to Identify ‘Critical Factors’for Development of Advanced MTDs

Previously reported MTDs were selected from a screen of more than 1,500signal peptide sequences. Although the MTDs that have been developed didnot have a common sequence or sequence motif, they were all derived fromthe hydrophobic (H) regions of signal sequences (HRSSs) that also lackcommon sequences or motifs except their hydrophobicity and the tendencyto adopt alpha-helical conformations. The wide variation in H-regionsequences may reflect prior evolution for proteins with membranetranslocating activity and subsequent adaptation to the SRP/Sec61machinery, which utilizes a methionine-rich signal peptide bindingpocket in SRP to accommodate a wide-variety of signal peptide sequences.

Previously described hydrophobic CPPs (e.g. MTS/MTM and MTD) werederived from the hydrophobic regions present in the signal peptides ofsecreted and cell surface proteins. The prior art consists first, of adhoc use of H-region sequences (MTS/MTM), and second, of H-regionsequences (with and without modification) with highest CPP activityselected from a screen of 1,500 signal sequences (MTM). Second priorart, the modified H-region derived hydrophobic CPP sequences hadadvanced in diversity with multiple number of available sequences apartfrom MTS/MTM derived from fibroblast growth factor (FGF) 4. However, thenumber of MTDs that could be modified from naturally occurring secretedproteins are somewhat limited. Because there is no set of rules indetermining their cell-permeability, no prediction for thecell-permeability of modified MTD sequences can be made before testingthem.

The hydrophobic CPPs, like the signal peptides from which theyoriginated, did not conform to a consensus sequence, and they hadadverse effects on protein solubility when incorporated into proteincargo. We therefore set out to identify optimal sequence and structuraldeterminants, namely critical factors (CFs), to design new hydrophobicCPPs with enhanced ability to deliver macromolecule cargoes includingproteins into the cells and tissues while maintaining proteinsolubility. These newly developed CPPs, advanced macromoleculetransduction domains (aMTDs) allowed almost infinite number of possibledesigns that could be designed and developed based on the criticalfactors. Also, their cell-permeability could be predicted by theircharacter analysis before conducting any in vitro and/or in vivoexperiments. These critical factors below have been developed byanalyzing all published reference hydrophobic CPPs.

1-1. Analysis of Hydrophobic CPPs

Seventeen different hydrophobic CPPs (Table 1) published from 1995 to2014 (Table 2) were selected. After physiological and chemicalproperties of selected hydrophobic CPPs were analyzed, 11 differentcharacteristics that may be associated with cell-permeability have beenchosen for further analysis. These 11 characteristics are as follows:sequence, amino acid length, molecular weight, pI value, bendingpotential, rigidity/flexibility, structural feature, hydropathy, residuestructure, amino acid composition and secondary structure of thesequences (Table 3).

Table 1 shows the summary of published hydrophobic Cell-PenetratingPeptides which were chosen.

TABLE 1 # Pepides Origin Protein Ref. 1 MTM Homo sapiens NP_001998Kaposi fibroblast growth factor (K-FGF) 1 2 MTS Homo sapiens NP_001998Kaposi fibroblast growth factor (K-FGF) 2 3 MTD10 Streptomycescoelicolor NP_625021 Glycosyl hydrolase 8 4 MTD13 Streptomycescoelicolor NP_639877 Putative secreted protein 3 5 MTD47 Streptomycescoelicolor NP_627512 Secreted protein 4 6 MTD56 Homo sapiens P23274Peptidyl-prolyl cis-trans isomerase B precursor 5 7 MTD73 Drosophilamelanogaster AAA17887 Spatzle (spz) protein 5 8 MTD77 Homo sapiensNP_003231 Kaposi fibroblast growth factor (K-FGF) 6 9 MTD84 Phytophthoracactorum AAK63068 Phytotoxic protein PcF precusor 4 10 MTD85Streptomyces coelicolor NP_629842 Peptide transport system peptidebinding 7 protein 11 MTD86 Streptomyces coelicolor NP_629842 Peptidetransport system secreted peptide 7 binding protein 12 MTD103 Homosapiens TMBV19 domain Family member B 8 13 MTD132 Streptomycescoelicolor NP_628377 P60-family secreted protein 4 14 MTD151Streptomyces coelicolor NP_630126 Secreted chitinase 8 15 MTD173Streptomyces coelicolor NP_624384 Secreted protein 4 16 MTD174Streptomyces coelicolor NP_733505 Large, multifunctional secretedprotein 8 17 MTD181 Neisseria meningitidis Z2491 CAB84257.1 Putativesecreted protein 4

Table 2 summarizes reference information

TABLE 2 References # Title Journal Year Vol Issue Page 1 Inhibition ofNuclear Translocation of Transcription Factor JOURNAL OF 1995 270 2414255 NF-kB by a Synthetic peptide Containing a Cell Membrane-BIOLOGICAL permeable Motif and Nuclear Localization Sequence CHEMISTRY 2Epigenetic Regulation of Gene Structure and Function with NATURE 2001 1910 929 a Cell-Permeable Cre Recombinase BIOTECHNOLOGY 3 Cell-PermeableNM23 Blocks the Maintenance and CANCER 2011 71 23 7216 Progression ofEstablished Pulmonary Metastasis RESEARCH 4 Antitumor Activity ofCell-Permeable p18INK4c With MOLECULAR 2012 20 8 1540 Enhanced Membraneand Tissue Penetration THERAPY 5 Antitumor Activity of Cell-PermeableRUNX3 Protein in CLINICAL 2012 19 3 680 Gastric Cancer Cells CANCERRESEARCH 6 The Effect of Intracellular Protein Delivery on the Anti-BIOMATERIALS 2013 34 26 6261 Tumor Activity of Recombinant HumanEndostatin 7 Partial Somatic to Stem Cell Transformations Induced BySCIENTIFIC 2014 4 10 4361 Cell-Permeable Reprogramming Factors REPORTS 8Cell-Permeable Parkin Proteins Suppress Parkinson PLOS ONE 2014 9 7 17Disease-Associated Phenotypes in Cultured Cells and Animals

Table 3 shows characteristics of published hydrophobic Cell-PenetratingPeptides (A) which were analyzed.

TABLE 3 Rigidity Flexi- Struc- bility tural SEQ Mol- Bending (insta-Feature ID Pep- ecular Po- bility (Aliphatic NOS tide Sequence LengthWeight pi tential Index: II) Index: AI) 865 MTM AAVALLPAVLLALLAP 161,615.9 5.6 Bending 45.5 220.0 866 MTS AAVLLPVLLAAP 12 1,147.4 5.6Bending 57.3 211.7 867 MTD10 LGGAVVAAPVAAAVAP 16 1,333.5 5.5 Bending47.9 140.6 868 MTD13 LAAAALAVLPL 11 1,022.3 5.5 Bending 26.6 213.6 869MTD47 AAAVPVLVAA 10   861.0 5.6 Bending 47.5 176.0 870 MTD56 VLLAAALIA 9  854.1 5.6 No- 6.9 250.0 Bending 871 MTD73 PVLLILAV 7   737.9 6.0 No-36.1 278.6 Bending 872 MTD77 AVALLILAV 9   882.0 5.6 No- 30.3 271.1Bending 873 MTD64 AVALVAVVAVA 11   962.2 5.6 No- 9.1 212.7 Bending 874MTD65 LLAAAAALLLA 11 1,010.2 5.5 No- 9.1 231.6 Bending 875 MTD66LLAAAAALLLA 11 1,010.2 5.5 No- 9.1 231.8 Bending 876 MTD103 LALPVLLLA 9  922.2 5.5 Bending 51.7 271.1 877 MTD132 AVVVPAVLAAP 12 1,119.4 5.6Bending 50.3 196.0 878 MTD161 AAAPVAAVP 9 1,031.4 5.6 Bending 73.1 120.0879 MTD173 AVIPILAVP 9   592.1 6.6 Bending 48.6 216.7 880 MTD174LILLLPAVALP 11 1,011.6 6.5 Bending 79.1 257.3 881 MTD181 AVLLLPAAA 9  838.0 5.6 Bending 51.7 206.7 AVE 10.8 ± 1,001 ± 5.6 ± Proline 40.1 ±217.9 ± 2.4 159.6 0.1 Presence 21.9 43.6 SEQ Hydro- A/a ID Pep- pathyResidue Composition Secondary NOS tide Sequence (GRAVY) Structure A V LI P G Structure Cargo Ref. 865 MTM AAVALLPAVL 2.4 Aliphatic 6 2 6 0 2 0Helix p50 1 LALLAP Ring 866 MTS AAVLLPVLLAAP 2.3 Aliphatic 4 2 4 0 2 0No- CRE 2 Ring Helix 867 MTD10 LGGAVVAAPVAA 1.8 Aliphatic 7 4 1 0 2 2Helix Parkin 8 AVAP Ring 868 MTD13 LAAAALAVLPL 2.4 Aliphatic 6 1 4 0 1 0No- RUNX3 5 Ring Helix 869 MTD47 AAAVPVLVAA 2.4 Aliphatic 5 3 1 0 1 0No- CMYC 7 Ring Helix 870 MTD56 VLLAAALIA 3.0 Aliphatic 4 1 3 1 0 0Helix ES 6 Ring 871 MTD73 PVLLILAV 2.8 Aliphatic 1 1 4 0 1 0 Helix ES 6Ring 872 MTD77 AVALLILAV 3.3 Aliphatic 3 2 3 1 0 0 Helix NV23 3 Ring 873MTD64 AVALVAVVAVA 3.1 Aliphatic 5 5 1 0 0 0 Helix OCT4 7 Ring 874 MTD65LLAAAAALLLA 2.7 Aliphatic 6 0 4 0 0 0 No- RUNX3 5 Ring Helix 875 MTD66LLAAAAALLLA 2.7 Aliphatic 6 0 5 0 0 0 No- SOX2 7 Ring Helix 876 MTD103LALPVLLLA 2.8 Aliphatic 2 1 5 0 1 0 Helix P18 4 Ring 877 MTD132AVVVPAVLAAP 2.4 Aliphatic 4 4 1 1 2 0 No- LIN26 7 Ring Helix 878 MTD161AAAPVAAVP 1.6 Aliphatic 5 2 0 0 2 0 No- Parkin 8 Ring Helix 879 MTD173AVIPILAVP 2.4 Aliphatic 2 2 1 2 2 0 Helix KLF4 7 Ring 880 MTD174LILLLPAVALP 2.6 Aliphatic 2 1 5 1 2 0 Helix Parkin 8 Ring 881 MTD181AVLLLPAAA 2.4 Aliphatic  4 1 3 0 1 0 No- SOX2 7 Ring Helix AVE 2.5 ± 0.4

Two peptide/protein analysis programs were used (ExPasy: SoSui:http://harrier.nagahama-i-bio.ac.jp/sosui/sosui_submit.html) todetermine various indexes and structural features of the peptidesequences and to design new sequence. Followings are important factorsanalyzed.

1-2. Characteristics of Analyzed Peptides: Length, Molecular Weight andPl Value

Average length, molecular weight and pl value of the peptides analyzedwere 10.8±2.4, 1,011±189.6 and 5.6±0.1, respectively (Table 4)

Table 4 summarizes Critical Factors (CFs) of published hydrophobicCell-Penetrating Peptides (A) which were analyzed.

TABLE 4 Length: 10.8 ± 2.4 Molecular Weight: 1,011 ± 189.6 pl: 5.6 ± 0.1Bending Potential (BP): Proline presences in the middle  and/or the endof peptides, or No Proline. Instability Index (II): 40.1 ± 21.9 ResidueStructure & Aliphatic Index (AI): 217.9 ± 43.6 Hydropathy (GRAVY): 2.5 ±0.4 Aliphatic Ring: Non-polar hydrophobic & aliphatic amino acid (A, V,L, I). Secondary Structure: α-Heliz is favored but not required.

1-3. Characteristics of Analyzed Peptides: Bending Potential—ProlinePosition (PP)

Bending potential (bending or no-bending) was determined based on thefact whether proline (P) exists and/or where the amino acid(s) providingbending potential to the peptide in recombinant protein is/are located.Proline differs from the other common amino acids in that its side chainis bonded to the backbone nitrogen atom as well as the alpha-carbonatom. The resulting cyclic structure markedly influences proteinarchitecture which is often found in the bends of folded peptide/proteinchain.

Eleven out of 17 were determined as ‘Bending’ peptide which means thatproline is present in the middle of sequence for peptide bending and/orlocated at the end of the peptide for protein bending. As indicatedabove, peptide sequences could penetrate the plasma membrane in a “bent”configuration. Therefore, bending or no-bending potential is consideredas one of the critical factors for the improvement of currenthydrophobic CPPs.

1-4. Characteristics of Analyzed Peptides:Rigidity/Flexibility—Instability Index (II)

Since one of the crucial structural features of any peptide is based onthe fact whether the motif is rigid or flexible, which is an intactphysicochemical characteristic of the peptide sequence, instabilityindex (II) of the sequence was determined. The index value representingrigidity/flexibility of the peptide was extremely varied (8.9-79.1), butaverage value was 40.1±21.9 which suggested that the peptide should besomehow flexible, but not too much rigid or flexible (Table 3).

1-5. Characteristics of Analyzed Peptides: StructuralFeatures—Structural Feature (Aliphatic Index: AI) and Hydropathy (GrandAverage of Hydropathy: GRAVY)

Alanine (V), valine (V), leucine (L) and isoleucine (I) containaliphatic side chain and are hydrophobic—that is, they have an aversionto water and like to cluster. These amino acids having hydrophobicityand aliphatic residue enable them to pack together to form compactstructure with few holes. Analyzed peptide sequence showed that allcomposing amino acids were hydrophobic (A, V, L and I) except glycine(G) in only one out of 17 (MTD10 (SEQ ID NO: 867)—Table 3) and aliphatic(A, V, L, I, and P). Their hydropathic index (Grand Average ofHydropathy: GRAVY) and aliphatic index (AI) were 2.5±0.4 and 217.9±43.6,respectively. Their amino acid composition is also indicated in theTable 3.

1-6. Characteristics of Analyzed Peptides: Secondary Structure(Helicity)

As explained above, the CPP sequences may be supposed to penetrate theplasma membrane directly after inserting into the membranes in a “bent”configuration with hydrophobic sequences having α-helical conformation.In addition, our analysis strongly indicated that bending potential wascrucial for membrane penetration. Therefore, structural analysis of thepeptides was conducted to determine whether the sequences were to formhelix or not. Nine peptides were helix and eight were not (Table 3). Itseems to suggest that helix structure may not be required.

1-7. Determination of Critical Factors (CFs)

In the 11 characteristics analyzed, the following 6 are selected namely“Critical Factors” for the development of new hydrophobic CPPs—advancedMTDs: amino acid length, bending potential (proline presence andlocation), rigidity/flexibility (instability index: II), structuralfeature (aliphatic index: AI), hydropathy (GRAVY) and amino acidcomposition/residue structure (hydrophobic and aliphatic A/a) (Table 3and Table 4).

2. Analysis of Selected Hydrophobic CPPs to Optimize ‘Critical Factors’

Since the analyzed data of the 17 different hydrophobic CPPs (analysisA, Table 3 and 4) previously developed during the past 2 decades showedhigh variation and were hard to make common- or consensus-features,analysis B (Table 5 and 6) and C (Table 7 and 8) were also conducted tooptimize the critical factors for better design of improved CPPs—aMTDs.Therefore, 17 hydrophobic CPPs have been grouped into two groups andanalyzed the groups for their characteristics in relation to the cellpermeable property. The critical factors have been optimized bycomparing and contrasting the analytical data of the groups anddetermining the common homologous features that may be critical for thecell permeable property.

2-1. Selective Analysis (B) of Peptides Used to Biologically ActiveCargo Protein for in Vivo

In analysis B, eight CPPs were used with each biologically active cargoin vivo. Length was 11±3.2, but 3 out of 8 CPPs possessed little bendingpotential. Rigidity/Flexibility (instability index: II) was 41±15, butremoving one [MTD85 (SEQ ID NO: 874): rigid, with minimal II (9.1)] ofthe peptides increased the overall instability index to 45.6±9.3. Thissuggested that higher flexibility (40 or higher II) is potentially bebetter. All other characteristics of the 8 CPPs were similar to theanalysis A, including structural feature and hydropathy (Table 5 and 6)

Table 5 shows characteristics of published hydrophobic Cell-PenetratingPeptides (B): selected CPPs that were used to each cargo in vivo.

TABLE 5 Rigidity Flexi- Struc- bility tural SEQ Mol- Bending (insta-Feature ID Pep- ecular Po- bility (Aliphatic NOS tides Sequence LengthWeight pi tential Index: II) Index: AI) 865 MTM AAVALLPAVLLA 16 1,515.95.6 Bending 45.5 220.0 LLAP 866 MTS AAVLLPVLLAAP 12 1,147.4 5.6 Bending57.3 211.7 867 MTD10 LGGAVVAAPVAA 16 1,333.5 5.5 Bending 47.9 140.6 AVAP871 MTD73 PVLLLLAV 7   737.9 6.0 No- 36.1 278.6 Bending 872 MTD77AVALLLLAV 9   882.1 5.6 No- 30.3 271.1 Bending 874 MTD85 LLAAAAALLLA 111,010.2 5.5 No- 9.1* 231.6 Bending 876 MTD103 LALPVLLLA 9   922.2 5.5Bending 51.7 271.1 877 MTD132 AVVVPAIVLAAP 12 1,119.4 5.6 Bending 50.3195.0 AVE 11 ± 1,083 ± 5.6 ± Proline 41 ± 227 ± 3.2 252 0.1 Presence 1547 SEQ Hydro- A/a ID Pep- pathy Residue Composition Secondary NOS tideSequence (GRAVY) Structure A V L I P G Structure Cargo Ref. 865 MTMAAVALLPAVLLA 2.4 Aliphatic 6 2 6 0 2 0 Helix p50 1 LLAP Ring 866 MTSAAVLLPVLLAAP 2.3 — 4 2 4 0 2 0 No-Helix CRE 2 867 MTD10 LGGAVVAAPVAA 1.8— 7 4 1 0 2 2 Helix Parkin 8 AVAP 871 MTD73 PVLLLLAV 2.8 — 1 1 4 0 1 0Helix ES 6 872 MTD77 AVALLILAV 3.3 — 3 2 3 1 0 0 Helix NM23 3 874 MTD65LLAAAAALLLA 2.7 — 6 0 5 0 0 0 No-Helix RUNX3 5 876 MTD103 LALPVLLLA 2.8— 2 1 5 0 1 0 Helix P18 4 877 MTD132 AVVVPAIVLAAP 2.4 — 4 4 1 1 2 0No-Helix LIN26 7 AVE 2.5 ± 0.4 *Removing the MTD85 increases II to 45.6± 9.3.

Table 6 shows summarized Critical Factors of published hydrophobicCell-Penetrating Peptides (B).

TABLE 6 Length: 11 ± 3.2 Molecular Weight: 1,083 ± 252 pl: 5.6 ± 0.1Bending Potential (BP): Praline presences in the middle  and/or the endof peptides, or Na Proline. Instabtlity Index (II): 41.0 ± 15 (′Removing the MTD85 increases II to 45.6 ± 9.3) Residue Structure &Aliphatic Index (AI): 227 ± 47 Hydropathy (GRAVY): 2.5 ± 0.4 AliphaticRing: Non-polar hydrophobic & aliphatic amino acid (A, V, L, I).Secondary Structure: α-Helix is favored but not required.

2-2. Selective Analysis (C) of Peptides that Provided Bending Potentialand Higher Flexibility

To optimize the ‘Common Range and/or Consensus Feature of CriticalFactor’ for the practical design of aMTDs and the random peptides (rPsor rPeptides), which were to prove that the ‘Critical Factors’determined in the analysis A, B and C were correct to improve thecurrent problems of hydrophobic CPPs—protein aggregation, lowsolubility/yield, and poor cell-/tissue-permeability of the recombinantproteins fused to the MTS/MTM or MTD, and non-common sequence andnon-homologous structure of the peptides, empirically selected peptideswere analyzed for their structural features and physicochemical factorindexes.

Hydrophobic CPPs which did not have a bending potential, rigid or toomuch flexible sequences (too much low or too much high InstabilityIndex), or too low or too high hydrophobic CPPs were unselected, butsecondary structure was not considered because helix structure ofsequence was not required.

In analysis C, eight selected CPP sequences that could provide a bendingpotential and higher flexibility were finally analyzed (Table 7 and 8).Common amino acid length is 12 (11.6±3.0). Proline is presence in themiddle of and/or the end of sequence. Rigidity/Flexibility (II) is45.5-57.3 (Avg: 50.1±3.6). AI and GRAVY representing structural featureand hydrophobicity of the peptide are 204.7±37.5 and 2.4±0.3,respectively. All peptides are consisted with hydrophobic and aliphaticamino acids (A, V, L, I, and P). Therefore, analysis C was chosen as astandard for the new design of new hydrophobic CPPs—aMTDs.

Table 7 shows characteristics of published hydrophobic Cell-PenetratingPeptides (C): selected CPPs that provided bending potential and higherflexibility.

TABLE 7 Rigidity Flexi- Struc- bility tural SEQ Mol- Bending (insta-Feature ID Pep- ecular Po- bility (Aliphatic NOS tide Sequence LengthWeight pi tential Index: II) Index: AI) 865 MTM AAVALLPAVLLA 16 1,515.95.6 Bending 45.5 220.0 LLAP 868 MTS AAVLLPVLLAAP 12 1,147.4 5.6 Bending57.3 211.7 867 MTD10 LGGAVVAAPVAA 16 1,333.5 5.5 Bending 47.9 140.6 AVAP869 MTD47 AAAVPVLVAA 10   881.0 5.6 Bending 47.5 176.0 876 MTD103LALPVLLLA 9   922.2 5.5 Bending 51.7 271.1 877 MTD132 AVVVPAIVLAAP 121,119.4 5.6 Bending 50.3 195.0 879 MTD173 AVIPILAVP 9   892.1 5.5Bending 48.5 215.7 881 MTD181 AVLLLPAAA 9   838.0 5.6 Bending 51.7 206.7AVE 11.6 ± 1,081 ± 5.6 ± Proline 50.1 ± 204.7 ± 3.0 244.6 0.1 Presence3.6 37.5 Secon- SEQ Hydro- A/a dary ID Pep- pathy Residue CompositionStruc- NOS tide Sequence (GRAVY) Structure A V L I P G ture Cargo Ref.865 MTM AAVALLPAVLL 2.4 Aliphatic 6 2 6 0 2 0 Helix p50 1 ALLAP Ring 868MTS AAVLLPVLLAAP 2.3 Aliphatic 4 2 4 0 2 0 No- CRE 2 Ring Helix 867MTD10 LGGAVVAAPVAA 1.8 Aliphatic 7 4 1 0 2 2 Helix PARKIN 8 AVAP Ring869 MTD73 AAAVPVLVAA 2.4 Aliphatic 5 3 1 0 1 0 No- CMYC 7 Ring Helix 876MTD77 LALPVLLLA 2.8 Aliphatic 2 1 5 0 1 0 Helix p18^(IHCAG) 4 Ring 877MTD65 AVVVPAIVLAAP 2.4 Aliphatic 4 4 1 1 2 0 No- LIN28 7 Ring Helix 879MTD103 AVIPILAVP 2.4 Aliphatic 2 2 1 2 2 0 Helix KLF4 7 Ring 881 MTD132AVLLLPAAA 2.4 Aliphatic 4 1 3 0 1 0 No- SOX2 7 Ring Helix AVE 2.4 ± 0.3

Table 8 shows summarized Critical Factors of published hydrophobicCell-Penetrating Peptides (C)

TABLE 8 Length: 11.6 ± 3.0 Molecular Weight. 1,081.2 ± 224.6 pl: 5.6 ±0.1 Bending Potential (BP): Proline presences in the middle  and/or theend of peptides. Instability Index (II): 50.1 ± 3.6 Residue Structure &Aliphatic Index (AI): 204.7 ± 37.5 Hydropathy (GRAVY): 2.4 ± 0.3Aliphatic Ring: Non-polar hydrophobic & aliphatic amino acid (A, V, L,I). Secondary Structure: α-Helix is favored but not required.3. New Design of Improved Hydrophobic CPPs—aMTDs Based on the OptimizedCritical Factors

3-1. Determination of Common Sequence and/or Common Homologous Structure

As mentioned above, H-regions of signal sequence (HRSS)-derived CPPs(MTS/MTM and MTD) do not have a common sequence, sequence motif, and/orcommon-structural homologous feature. In this invention, the aim is todevelop improved hydrophobic CPPs formatted in the common sequence- andstructural-motif which satisfy newly determined ‘Critical Factors’ tohave ‘Common Function,’ namely, to facilitate protein translocationacross the membrane with similar mechanism to the analyzed referenceCPPs. Based on the analysis A, B and C, the common homologous featureshave been analyzed to determine the critical factors that influence thecell-permeability. The range value of each critical factor has beendetermined to include the analyzed index of each critical factor fromanalysis A, B and C to design novel aMTDs (Table 9). These features havebeen confirmed experimentally with newly designed aMTDs in theircell-permeability.

Table 9 shows comparison the range/feature of each Critical Factorbetween the value of analyzed CPPs and the value determined for newdesign of novel aMTDs sequences

TABLE 9 Summarized Critical Factors of aMTD Selected CPPs Newly DesignedCPPs Critical Factor Range Range Bending Potential Proline presences inProline presences in the (Proline Position: PP) the middle and/or atmiddle (5′, 6′, 7′ or 8′) the end of peptides end at the end of peptidesRigidity/Flexibility 45.5-57.3 40-60 (Instability Index: II) (50.1 ±3.6)  Structural Feature 140.6-220.0 180-220 (Aliphatic Index: AI)(204.7 ± 37.5)  Hydropathy 1.8-2.8 2.1-2.6 (Grand Average of (2.4 ± 0.3)Hydropathy GRAVY) Length (Number of Amino Acid) 11.6 ± 3.0  9-13 Aminoacid Composition A, V, I, L, P A, V, I, L, P

In Table 9, universal common features and sequence/structural motif areprovided. Length is 9-13 amino acids, and bending potential is providedwith the presence of proline in the middle of sequence (at 5′, 6′, 7′ or8′ amino acid) for peptide bending and at the end of peptide forrecombinant protein bending and Rigidity/Flexibility of aMTDs is II>40are described in Table 9.

3-2. Critical Factors for Development of Advanced MTDs

Recombinant cell-permeable proteins fused to the hydrophobic CPPs todeliver therapeutically active cargo molecules including proteins intolive cells had previously been reported, but the fusion proteinsexpressed in bacteria system were hard to be purified as a soluble formdue to their low solubility and yield. To address the crucial weaknessfor further clinical development of the cell-permeable proteins asprotein-based biotherapeutics, greatly improved form of the hydrophobicCPP, named as advanced MTD (aMTD) has newly been developed throughcritical factors-based peptide analysis. The critical factors used forthe current invention of the aMTDs are herein (Table 9).

1. Amino Acid Length: 9-13

2. Bending Potential (Proline Position: PP)

: Proline presences in the middle (from 5′ to 8′ amino acid) and at theend of sequence

3. Rigidity/Flexibility (Instability Index: II): 40-60

4. Structural Feature (Aliphatic Index: AI): 180-220

5. Hydropathy (GRAVY): 2.1-2.6

6. Amino Acid Composition: Hydrophobic and Aliphatic amino acids—A, V,L, I and P

3-3. Design of Potentially Best aMTDs that all Critical Factors areConsidered and Satisfied

After careful consideration of six critical factors derived fromanalysis of unique features of hydrophobic CPPs, advanced macromoleculetransduction domains (aMTDs) have been designed and developed based onthe common 12 amino acid platform which satisfies the critical factorsincluding amino acid length (9-13) determined from the analysis.

Unlike previously published hydrophobic CPPs that require numerousexperiments to determine their cell-permeability, newly developed aMTDsequences could be designed by performing just few steps as followsusing above mentioned platform to follow the determined rangevalue/feature of each critical factor.

First, prepare the 12 amino acid sequence platform for aMTD. Second,place proline (P) in the end (12′) of sequence and determine where toplace proline in one of four U(s) in 5′, 6′, 7′, and 8. Third, alanine(A), valine (V), leucine (L) or isoleucine (I) is placed in either X(s)and/or U(s), where proline is not placed. Lastly, determine whether theamino acid sequences designed based on the platform, satisfy the valueor feature of six critical factors to assure the cell permeable propertyof aMTD sequences. Through these processes, numerous novel aMTDsequences have been constructed. The expression vectors for preparingnon-functional cargo recombinant proteins fused to each aMTD, expressionvectors have been constructed and forcedly expressed in bacterial cells.These aMTD-fused recombinant proteins have been purified in soluble formand determined their cell-permeability quantitatively. aMTD sequenceshave been newly designed, numbered from 1 to 240, as shown in Table10-15. In Table 10-15, sequence ID Number is a sequence listings forreference, and aMTD numbers refer to amino acid listing numbers thatactually have been used at the experiments. For further experiments,aMTD numbers have been used. In addition, polynucleotide sequences shownin the sequence lists have been numbered from SEQ ID NO: 241 to SEQ IDNO: 480.

Tables 10 to 15 shows 240 new hydrophobic aMTD sequences that weredeveloped to satisfy all critical factors.

TABLE 10 Rigidity Structural Sequence Flexibility Feature HydropathyResidue ID Number aMTD Sequences Length (II) (AI) (GRAVY) Structure  1 1 AAALAPVVLALP 12 57.3 187.5 2.1 Aliphatic  2  2 AAAVPLLAVVVP 12 41.3195.0 2.4 Aliphatic  3  3 AALLVPAAVLAP 12 57.3 187.5 2.1 Aliphatic  4  4ALALLPVAALAP 12 57.3 195.2 2.1 Aliphatic  5  5 AAALLPVALVAP 12 57.3187.5 2.1 Aliphatic  6 11 VVALAPALAALP 12 57.3 187.5 2.1 Aliphatic  7 12LLAAVPAVLLAP 12 57.3 211.7 2.3 Aliphatic  8 13 AAALVPVVALLP 12 57.3203.3 2.3 Allphatk  9 21 AVALLPALLAVP 12 57.3 211.7 2.3 Ailphatic 10 22AVVLVPVLAAAP 12 57.3 195.0 2.4 Aliphatic 11 23 VVLVLPAAAAVP 12 57.3195.0 2.4 Aliphatic 12 24 IALAAPALIVAP 12 50.2 195.8 2.2 Aliphatic 13 25IVAVAPALVALP 12 50.2 203.3 2.4 Aliphatic 14 42 VAALPVVAVVAP 12 57.3196.7 2.4 Aliphatic 15 43 LLAAPLVVAAVP 12 41.3 187.5 2.1 Aliphatic 16 44ALAVPVALLVAP 12 57.3 203.3 2.3 Aophatic 17 61 VAALPVLLAALP 12 57.3 211.72.3 Aliphatic 18 62 VALLAPVALAVP 12 57.3 203.3 2.3 Aliphatic 19 63AALLVPALVAVP 12 57.3 203.3 2.3 Aliphatic

TABLE 11 Rigidity/ Structural Sequence Flexibility Feature HydropathyResidue ID Number aMTD Sequences Length (II) (AI) (GRAVY) Structure 2064 AIVALPVAVLAP 12 50.2 203.3 2.4 Aliphatic 21 65 IAIVAPVVALAP 12 50.2203.3 2.4 Aliphatic 22 81 AALLPALAALLP 12 57.3 204.2 2.1 Aliphatic 23 82AVVLAPVAAVLP 12 57.3 195.0 2.4 Aliphatic 24 83 LAVAAPLALALP 12 41.3195.8 2.1 Aliphatic 25 84 AAVAAPLLLALP 12 41.3 195.8 2.1 Aliphatic 26 85LLVLPAAALAAP 12 57.3 195.8 2.1 Aliphatic 27 101 LVALAPVAAVLP 12 57.3203.3 2.3 Aliphatic 28 102 LALAPAALALLP 12 57.3 204.2 2.1 Aliphatic 29100 ALIAAPILALAP 12 57.3 204.2 2.2 Aliphatic 30 104 AVVAAPLVLALP 12 41.3203.3 2.3 Aliphatic 31 105 LLALAPAALLAP 12 57.3 204.1 2.1 Aliphatic 32121 AIVALPALALAP 12 50.2 195.8 2.2 Aliphatic 33 123 AAIIVPAALLAP 12 50.2195.8 2.2 Aliphatic 34 124 IAVALPALIAAP 12 50.3 195.8 2.2 Aliphatic 35141 AVIVLPALAVAP 12 50.2 203.3 2.4 Aliphatic 36 143 AVLAVPAVLVAP 12 57.3195.0 2.4 Aliphatic 37 144 VLAIVPAVALAP 12 50.2 203.3 2.4 Aliphatic 38145 LLAVVPAVALAP 12 57.3 203.3 2.3 Aliphatic 39 161 AVIALPALIAAP 12 57.3196.8 2.2 Aliphatic 40 152 AVVALPAALIVP 12 50.2 203.3 2.4 Aliphatic 41163 LALVLPAALAAP 12 57.3 195.8 2.1 Aliphatic 42 164 LAAVLPALLAAP 12 57.3195.8 2.1 Aliphatic 43 165 ALAVPVALAIVP 12 50.2 203.3 2.4 Aliphatic 44182 ALAIPVVALVAP 12 57.3 203.3 2.4 Aliphatic 45 183 LLAAPVVIALAP 12 57.3211.6 2.4 Aliphatic 46 184 LAAIVPAIIAVP 12 50.2 211.6 2.4 Aliphatic 47185 AALVLPLIIAAP 12 41.3 220.0 2.4 Aliphatic 48 201 LALAVPALAALP 12 57.3195.8 2.1 Aliphatic 49 204 LIAALPAVAALP 12 57.3 195.8 2.2 Aliphatic 50205 ALALVPAIAALP 12 57.3 195.8 2.2 Aliphatic 51 221 AAILAPIVALAP 12 50.2195.8 2.2 Aliphatic 52 222 ALLIAPAAVIAP 12 57.3 195.8 2.2 Aliphatic 53223 AILAVPIAVVAP 12 57.3 203.3 2.4 Aliphatic 54 224 ILAAVPIALAAP 12 57.3195.8 2.2 Aliphatic 55 225 VAALLPAAAVLP 12 57.3 187.5 2.1 Aliphatic 56241 AAVVPVLLVAP 12 57.3 195.0 2.4 Aliphatic 57 242 AALLVPALVAAP 12 57.3187.5 2.1 Aliphatic 58 243 AAVLLPVALAAP 12 57.3 187.5 2.1 Aliphatic 59245 AAALAPVLALVP 12 57.3 187.5 2.1 Aliphatic 60 261 LVLVPLLAAAAP 12 41.3211.6 2.3 Aliphatic 61 262 ALIAVPAIIVAP 12 50.2 211.6 2.4 Aliphatic 62263 ALAVIPAAAILP 12 54.9 195.8 2.2 Aliphatic 63 264 LAAAPVVIVIAP 12 50.2203.3 2.4 Aliphatic 64 265 VLAIAPLLAAVP 12 41.3 211.6 2.3 Aliphatic 65281 ALIVLPAAVAVP 12 50.2 203.3 2.4 Aliphatic 66 282 VLAVAPALIVAP 12 50.2203.3 2.4 Aliphatic 67 283 AALLAPALIVAP 12 50.2 196.8 2.2 Aliphatic 68284 ALIAPAVALIVP 12 50.2 211.7 2.4 Aliphatic 69 285 AIVLLPAAVVAP 12 50.2203.3 2.4 Aliphatic

TABLE 12 Rigidity/ Structural Sequence Flexibility Feature HydropathyResidue ID Number aMTD Sequences Length (II) (AI) (GRAVY) Structure  70301 VIAAPVLAVLAP 12 57.3 203.3 2.4 Aliphatic  71 302 LALAPALALLAP 1257.3 204.2 2.1 Aliphatic  72 304 AIILAPIAAIAP 12 57.3 204.2 2.3Aliphatic  73 305 IALAAPILLAAP 12 57.3 204.2 2.2 Aliphatic  74 321IVAVALPALAVP 12 50.2 203.3 2.3 Aliphatic  75 322 VVAILVLPALAAP 12 50.2203.3 2.3 Aliphatic  76 323 IVAVALPVALAP 12 50.2 203.3 2.3 Aliphatic  77324 IVAVALPAALVP 12 50.2 203.3 2.3 Aliphatic  78 325 IVAVALPAVALP 1250.2 203.3 2.3 Aliphatic  79 341 IVAVALPAVLAP 12 50.2 203.3 2.3Aliphatic  80 342 VIVALAPAVLAP 12 50.2 203.3 2.3 Aliphatic  81 343IVAVALPALVAP 12 50.2 203.3 2.3 Aliphatic  82 345 ALLIVAPVAVAP 12 50.2203.3 2.3 Aliphatic  83 361 AVVIVAPAVIAP 12 50.2 195.0 2.4 Aliphatic  84363 AVLAVAPALIVP 12 50.2 203.3 2.3 Aliphatic  85 364 LVAAVAPALIVP 1250.2 203.3 2.3 Aliphatic  86 365 AVIVVAPALLAP 12 50.2 203.3 2.3Aliphatic  87 381 VVAIVLPAVAAP 12 50.2 195.0 2.4 Aliphatic  88 382AAALVIPAILAP 12 54.9 195.8 2.2 Aliphatic  89 383 VIVALAPALLAP 12 50.2211.6 2.3 Aliphatic  90 384 VIVAIAPALLAP 12 50.2 211.6 2.4 Aliphatic  91385 IVAIAVPALVAP 12 50.2 203.3 2.4 Aliphatic  92 401 AALAVIPAAILP 1254.9 195.8 2.2 Aliphatic  93 402 ALAAVIPAAILP 12 54.9 195.8 2.2Aliphatic  94 403 AAALVIPAAILP 12 54.9 195.8 2.2 Aliphatic  95 404LAAaVIPAAILP 12 54.9 195.8 2.2 Aliphatic  96 405 LAAAVIPVAILP 12 54.9211.7 2.4 Aliphatic  97 421 AAILAAPLIAVP 12 57.3 195.8 2.2 Aliphatic  98422 VVAILAPLLAAP 12 57.3 211.7 2.4 Aliphatic  99 424 AVVVAAPVLALP 1257.3 195.0 2.4 Aliphatic 100 425 AVVAIAPVLALP 12 57.3 203.3 2.4Aliphatic 101 442 ALAALVPAVLVP 12 57.3 203.3 2.3 Aliphatic 102 443ALAALVPVALVP 12 57.3 203.3 2.3 Aliphatic 103 444 LAAALVPVALVP 12 57.3203.3 2.3 Aliphatic 104 445 ALAALVPALVVP 12 57.3 203.3 2.3 Aliphatic 105461 IAAVIVPAVALP 12 50.2 203.3 2.4 Aliphatic 106 462 IAAVLVPAVALP 1257.3 203.3 2.4 Aliphatic 107 463 AVAILVPLLAAP 12 57.3 211.7 2.4Aliphatic 108 464 AVVILVPLAAAP 12 57.3 203.3 2.4 Aliphatic 109 465IAAVIVPCAALP 12 50.2 203.3 2.4 Aliphatic 110 481 AIAIAIVPVALP 12 50.2211.6 2.4 Aliphatic 111 482 ILAVAAIPVAVP 12 54.9 203.3 2.4 Aliphatic 112483 ILAAAIIPAALP 12 54.9 204.1 2.2 Aliphatic 113 484 LAVVLAAPAIVP 1250.2 203.3 2.4 Aliphatic 114 485 AILAAIVPLAVP 12 50.2 211.6 2.4Aliphatic 115 501 VIVALAVPALAP 12 50.2 203.3 2.4 Aliphatic 116 502AIVALAVPVLAP 12 50.2 203.3 2.4 Aliphatic 117 503 AAIIVLPAALP 12 50.2220.0 2.4 Aliphatic 118 504 LIVALAVPALAP 12 50.2 211.7 2.4 Aliphatic 119505 AIIIVIAPAAAP 12 50.2 195.8 2.3 Aliphatic

TABLE 13 Rigidity/ Structural Sequence Len- Flexibility FeatureHydropathy Residue ID Number aMTD Sequences gth (II) (AI) (GRAVY)Structure 120 521 LAALIVVPAVAP 12 50.2 203.3 2.4 Aliphatic 121 522ALLVIAVPAVAP 12 57.3 203.3 2.4 Aliphatic 122 524 AVALIVVPALAP 12 50.2203.3 2.4 Aliphatic 123 525 ALAIVVAPVAVP 12 50.2 195.0 2.4 Aliphatic 124541 LLALIIAPAAAP 12 57.3 204.1 2.1 Aliphatic 125 542 ALALIIVPAVAP 1250.2 211.6 2.4 Aliphatic 126 543 LLAALIAPAALP 12 57.3 204.1 2.1Aliphatic 127 544 IVALIVAPAAVP 12 43.1 203.3 2.4 Aliphatic 128 545VVLVLAAPAAVP 12 57.3 195.0 2.3 Aliphatic 129 561 AAVAIVLPAVVP 12 50.2195.0 2.4 Aliphatic 130 562 ALIAAIVPALVP 12 50.2 211.7 2.4 Aliphatic 131563 ALAVIVVPALAP 12 50.2 203.3 2.4 Aliphatic 132 564 VAIALIVPALAP 1250.2 211.7 2.4 Aliphatic 133 565 VAIVLVAPAVAP 12 502 195.0 2.4 Aliphatic134 582 VAVALIVPALAP 12 50.2 203.3 2.4 Aliphatic 135 583 AVILALAPIVAP 1250.2 211.6 2.4 Aliphatic 136 585 ALIVAIPALVP 12 50.2 211.6 2.4 Aliphatic137 601 AAILIAVPIAAP 12 57.3 195.8 2.3 Aliphatic 138 602 VIVALAAPVLAP 1250.2 203.3 2.4 Aliphatic 139 603 VLVALAAPVIAP 12 57.3 203.3 2.4Aliphatic 140 604 VALIAVAPAVVP 12 57.3 195.0 2.4 Aliphatic 141 605VIAAVLAPVAVP 12 57.3 195.0 2.4 Aliphatic 142 622 ALIVLAAPVAVP 12 50.2203.3 2.4 Aliphatic 143 623 VAAAIALPAIVP 12 50.2 187.5 2.3 Aliphatic 144625 ILAAAAAPLIVP 12 50.2 195.8 2.2 Aliphatic 145 643 LALVLAAPAIVP 1250.2 211.6 2.4 Aliphatic 146 645 ALAVVALPAIVP 12 50.2 203.3 2.4Aliphatic 147 661 AAILAPIVAALP 12 50.2 195.8 2.2 Aliphatic 148 664ILIAIAPAAAP 12 54.9 204.1 2.3 Aliphatic 149 665 LAIVLAAPVAVP 12 50.2203.3 2.3 Aliphatic 150 666 AAIAIIAPAIVP 12 50.2 195.8 2.3 Aliphatic 151667 LAVAIVAPALVP 12 50.2 203.3 2.3 Aliphatic 152 683 LAIVLAAPAVLP 1250.2 211.7 2.4 Aliphatic 153 684 AAIVLALPAVLP 12 50.2 211.7 2.4Aliphatic 154 685 ALLVAVLPAALP 12 57.3 211.7 2.3 Aliphatic 155 686AALVAVLPVALP 12 57.3 203.3 2.3 Aliphatic 156 687 AILAVALPLLAP 12 57.3220.0 2.3 Aliphatic 157 703 IVAVALVPALAP 12 50.2 203.3 2.4 Aliphatic 158705 IVAVALLPALAP 12 50.2 211.7 2.4 Aliphatic 159 706 IVAVALLPAVAP 1250.2 203.3 2.4 Aliphatic 160 707 IVALAVLPAVAP 12 50.2 203.3 2.4Aliphatic 161 724 VAVLAVLPALAP 12 57.3 203.3 2.3 Aliphatic 162 725IAVLAVAPAVLP 12 57.3 203. 2.3 Aliphatic 163 726 LAVAIIAPAVAP 12 57.3187.5 2.2 Aliphatic 164 727 VALAIALPAVLP 12 57.3 211.6 2.3 Aliphatic 165743 AIAIALVPVALP 12 57.3 211.6 2.4 Aliphatic 166 744 AAVVIVAPVALP 1250.2 195.0 2.4 Aliphatic 167 746 VAIIVVAPALAP 12 50.2 203.3 2.4Aliphatic 168 747 VALLAIAPALAP 12 57.3 195.8 2.2 Aliphatic 169 763VAVLIAVPALAP 12 57.3 23.3 2.3 Aliphatic

TABLE 14 Rigidity/ Structural Hydro- Sequence Len- Flexibility Featurepathy Residue ID Number aMTD Sequences gth (II) (AI) (GRAVY) Structure170 764 AVALAVLPAVVP 12 57.3 195.0 2.3 Aliphatic 171 765 AVALAVVPAVLP 1257.3 195.0 2.3 Aliphatic 172 766 IVVIAVAPAVAP 12 50.2 195.0 2.4Aliphatic 173 767 IVVAAVVPALAP 12 50.2 195.0 2.4 Aliphatic 174 783IVALVPAVAIAP 12 50.2 203.3 2.5 Aliphatic 175 784 VAALPAVALVVP 12 57.3195.0 2.4 Aliphatic 176 786 LVAIAPLAVLAP 12 41.3 211.7 2.4 Aliphatic 177787 AVALVPVIVAAP 12 50.2 195.0 2.4 Aliphatic 178 788 AIAVAIAPVALP 1257.3 187.5 2.3 Aliphatic 179 803 AIALAVPVLALP 12 57.3 211.7 2.4Aliphatic 180 805 LVLIAAAPIALP 12 41.3 220.0 2.4 Aliphatic 181 806LVALAVPAAVLP 12 57.3 203.3 2.3 Aliphatic 182 807 AVALAVPALVL 12 57.3203.3 2.3 Aliphatic 183 808 LVVLAAAPLAVP 12 41.3 203.3 2.3 Aliphatic 184809 LIVLAAPALAAP 12 50.2 195.8 2.2 Aliphatic 185 810 VIVLAAPALAAP 1250.2 187.5 2.2 Aliphatic 186 811 AVVLAVPALAVP 12 57.3 195.0 2.3Aliphatic 187 824 LIIVAAAPAVAP 12 50.2 187.5 2.3 Aliphatic 188 825IVAVIVAPAVAP 12 43.2 195.0 2.5 Aliphatic 189 826 LVALAAPIIAVP 12 41.3211.7 2.4 Aliphatic 190 827 IAAVLAAPALVP 12 57.3 187.5 2.2 Aliphatic 191828 IALLAAPIIAVP 12 41.3 220.0 2.4 Aliphatic 192 829 AALALVAPVIVP 1250.2 203.3 2.4 Aliphatic 193 830 IALVAAPVALVP 12 57.3 203.3 2.4Aliphatic 194 831 IIVAVAPAAIVP 12 43.2 203.3 2.5 Aliphatic 195 832AVAAIVPVIVAP 12 43.2 195.0 2.5 Aliphatic 196 843 AVLVLVAPAAAP 12 41.3219.2 2.5 Aliphatic 197 844 VVALLAPLIAAP 12 41.3 211.8 2.4 Aliphatic 198845 AAVVIAPLLAVP 12 41.3 203.3 2.4 Aliphatic 199 846 IAVAVAAPLLVP 1241.3 203.3 2.4 Aliphatic 200 847 LVAIVVLPAVAP 12 50.2 219.2 2.6Aliphatic 201 848 AVAIVVLPAVAP 12 50.2 195.0 2.4 Aliphatic 202 849AVILLAPLIAAP 12 57.3 220.0 2.4 Aliphatic 203 850 LVIALAAPVALP 12 57.3211.7 2.4 Aliphatic 204 851 VLAVVLPAVALP 12 57.3 219.2 2.5 Aliphatic 205852 VLAVAAPAVLLP 12 57.3 203.3 2.3 Aliphatic 206 863 AAVVLLPIIAAP 1241.3 211.7 2.4 Aliphatic 207 864 ALLVIAPAIAVP 12 57.3 211.7 2.4Aliphatic 208 865 AVLVIAVPAIAP 12 57.3 203.3 2.5 Aliphatic 209 867ALLVVIAPLAAP 12 41.3 211.7 2.4 Aliphatic 210 868 VLVAAILPAAIP 12 54.9211.7 2.4 Aliphatic 211 870 VLVAAVLPIAAP 12 41.3 203.3 2.4 Aliphatic 212872 VLAAAVLPLVVP 12 41.3 219.2 2.5 Aliphatic 213 875 AIAIVVPAVAVP 1250.2 195.0 2.4 Aliphatic 214 877 VAIIAVPAVVAP 12 57.3 195.0 2.4Aliphatic 215 878 IVALVAPAAVVP 12 50.2 195.0 2.4 Aliphatic 216 879AAIVLLPAVVVP 12 50.2 219.1 2.5 Aliphatic 217 881 AALIVVPAVAVP 12 50.2195.0 2.4 Aliphatic 218 882 AIALVVPAVAVP 12 57.3 195.0 2.4 Aliphatic 219883 LAIVPAAIAALP 12 50.2 195.8 2.2 Aliphatic

TABLE 15 Rigidity/ Structural Hydro- Sequence Len- Flexibility Featurepathy Residue ID Number aMTD Sequences gth (II) (AI) (GRAVY) Structure220 885 LVAIAPAVAVLP 12 57.3 203.3 2.4 Aliphatic 221 887 VLAVAPAVAVLP 1257.3 195.0 2.4 Aliphatic 222 888 ILAVVAIPAAAP 12 54.9 187.5 2.3Aliphatic 223 889 ILVAAAPIAALP 12 57.3 195.8 2.2 Aliphatic 224 891ILAVAAIPAALP 12 54.9 195.8 2.2 Aliphatic 225 893 VIAIPAILAAAP 12 54.9195.8 2.3 Aliphatic 226 895 AIIIVVPAIAAP 12 50.2 211.7 2.5 Aliphatic 227896 AILIVVAPIAAP 12 50.2 211.7 2.5 Aliphatic 228 897 AVIVPVAIIAAP 1250.2 203.3 2.5 Aliphatic 229 899 AVVIALPAVVAP 12 57.3 195.0 2.4Aliphatic 230 900 ALVAVIAPVVAP 12 57.3 195.0 2.4 Aliphatic 231 901ALVAVLPAVAVP 12 57.3 195.0 2.4 Aliphatic 232 902 ALVAPLLAVAVP 12 41.3203.3 2.3 Aliphatic 233 904 AVLAVVAPVVAP 12 57.3 186.7 2.4 Aliphatic 234905 AVIAVAPLVVAP 12 41.3 195.0 2.4 Aliphatic 235 906 AVIALAPVVVAP 1257.3 195.0 2.4 Aliphatic 236 907 VAIALAPVVVAP 12 57.3 195.0 2.4Aliphatic 237 908 VALALAPVVVAP 12 57.3 195.0 2.3 Aliphatic 238 910VAALLPAVVVAP 12 57.3 195.0 2.3 Aliphatic 239 911 VALALPAVVVAP 12 57.3195.0 2.3 Aliphatic 240 912 VALLAPAVVVAP 12 57.3 195.0 2.3 Aliphatic52.6 ± 5.1 201.7 ± 7.8 2.3 ± 0.1

3-4. Design of the Peptides that Did not Satisfy at Least One CriticalFactor

To demonstrate that this invention of new hydrophobic CPPs-aMTDs, whichsatisfy all critical factors described above, are correct and rationallydesigned, the peptides which do not satisfy at least one critical factorhave also been designed. Total of 31 rPeptides (rPs) are designed,developed and categorized as follows: no bending peptides, either noproline in the middle as well at the end and/or no central proline;rigid peptides (II<40); too much flexible peptides; aromatic peptides(aromatic ring presences); hydrophobic, with non-aromatic peptides buthave amino acids other than A, V, L, I, P or additional prolineresidues; hydrophilic, but non-aliphatic peptides.

3-4-1. Peptides that do not Satisfy the Bending Potential

Table 16 shows the peptides that do not have any proline in the middle(at 5′, 6′, 7′ or 8′) and at the end of the sequences. In addition,Table 16 describes the peptides that do not have proline in the middleof the sequences. All these peptides are supposed to have no-bendingpotential.

TABLE 16 rPeptide Proline Rigidity/ Structural Hydro- ID (SEQ Len-Position Flexibility Feature pathy Group  ID NO) Sequences gth (PP) (II)(AI) (GRAVY) No-Bending Peptides 931 (882) AVLIAPAILAAA 12  6 57.3 204.22.5 [No Proline at 5, 936 (883) ALLILAAAVAAP 12 12 41.3 204.2 2.46, 7 or 8  152 (884) LAAAVAAVAALL 12 None 9.2 204.2 2.7 and/or 12]27 (885) LAIVAAAAALVA 12 None 2.1 204.2 2.8 935 (886) ALLILPAAAVAA 12  6 57.3 204.2 2.4 670 (887) ALLILAAAVAAL 12 None 25.2 236.6 2.8934 (888) LILAPAAVVAAA 12 5 57.3 195.8 2.5 37 (889) TTCSQQQYCTNG 12 None53.1 0.0 −1.1 16 (890) NNSCTTYTNGSQ 12 None 47.4 0.0 −1.4 113 (891)PVAVALLIAVPP 12 1, 11, 57.3 195.0 2.1 12

3-4-2. Peptides that do not Satisfy the Rigidity/Flexibility

To prove that rigidity/flexibility of the sequence is a crucial criticalfactor, rigid (Avg. II: 21.8±6.6) and too high flexible sequences (Avg.II: 82.3±21.0) were also designed. Rigid peptides that instability indexis much lower than that of new aMTDs (II: 41.3-57.3, Avg. II: 53.3±5.7)are shown in Table 17. Bending, but too high flexible peptides that IIis much higher than that of new aMTDs are also provided in Table 18.

TABLE 17 rPeptide Proline Rigidity/ Structural Hydro- ID (SEQ Len-Position Flexibility Feature pathy Group ID No) Sequences gth (PP) (II)(AI) (GRAVY) Rigid 226 (892) ALVAAIPALAIP 12 6 20.4 195.8 2.2 peptides  6 (893) VIAMIPAAFVVVA 12 6 15.7 146.7 2.2 (II < 50) 750 (894)LAIAAIAPLAIP 12 8, 12 22.8 204.2 2.2  26 (895) AAIALAAPLAIV 12 8 18.1204.2 2.5 527 (896) LVLAAVAPIAIP 12 8, 12 22.8 211.7 2.4 466 (897)IIAAAAPLAIIIP 12 7, 12 22.8 204.2 2.3 167 (898) VAIAIPAALAIP 12 6, 1220.4 195.8 2.3 246 (899) VVAVPLLVAFAA 12 5 25.2 195.0 2.7 426 (900)AAALAIPLAIIP 12 7, 12 4.37 204.2 2.2 606 (901) AAAIAAIPIIIP 12 8, 12 4.4204.2 2.4  66 (902) AGVLGGPIMGVP 12 7, 12 35.5 121.7 1.3 248 (903)VAAIVPIAALVP 12 6, 12 34.2 203.3 2.5 227 (904) LAAIVPIAAVP 12 6, 12 34.2187.5 2.2  17 (905) GGCSAPQTTCSN 12 6 51.6 8.3 −0.5  67 (906)LDAEVPLADDVP 12 6, 12 34.2 130 0.3

TABLE 18 rPeptide Proline Rigidity/ Structural Hydro- ID (SEQ Len-Position Flexibility Feature pathy Group ID NO) Sequences gth (PP) (II)(AI) (GRAVY) Bending 692 (907) PAPLPPVVILAV 12 1, 3, 5, 6 105.5 186.71.8 Peptides  69 (908) PVAVLPPAALVP 12 1, 6, 7, 12 89.4 162.5 1.6but Too 390 (909) VPLLVPVVPVVP 12 2, 6, 9, 12 105.4 210.0 2.2 High350 (910) VPILVPVVPVVP 12 2, 6, 9, 12 121.5 210.0 2.2 Flexi- 331 (911)VPVLVPLVPVVP 12 2, 6, 9, 12 105.4 210.0 2.2 bility   9 (912)VALVPAALILPP 12 5, 11, 12 89.4 203.3 2.1  68 (913) VAPVLPAAPLVP 123, 6, 9, 12 105.5 162.5 1.6 349 (914) VPVLVPVVPVVP 12 2, 6, 9, 12 121.5201.6 2.2 937 (915) VPVLVPLPVPVV 12 2, 6, 8, 10 121.5 210.0 2.2938 (916) VPVLLPVVVPVP 12 2, 6, 121.5 210.0 2.2 10, 12 329 (917)LPVLVPVVPVVP 12 2, 6, 9, 12 121.5 210.0 2.2  49 (918) VVPAAPAVPVVP 123, 6, 9, 12 121.5 145.8 1.7 772 (919) LPVAPVIPIIVP 12 2, 5, 8, 12 79.9210.8 2.1 210 (920) ALIALPALPALP 12 6, 9, 12 89.4 195.5 1.8  26 (921)AVPLLPLVPAVP 12 3, 6, 9, 12 89.4 185.8 1.8 693 (922) AAPVLPVAVPIV 123, 6, 10 82.3 185.7 2.1 169 (923) VALVAPALILAP 12 6, 12 73.4 211.7 2.4 29 (924) VLPPLPVLPVLP 12 3, 4  121.5 202.5 1.7 6, 9, 12 190 (925)AAILAPAVIAPP 12 6, 11, 12 89.4 163.3 1.8

3-4-3. Peptides that do not Satisfy the Structural Features

New hydrophobic CPPs-aMTDs are consisted with only hydrophobic andaliphatic amino acids (A, V, L, I and P) with average ranges of theindexes—AI: 180-220 and GRAVY: 2.1-2.6 (Table 9). Based on thestructural indexes, the peptides which contain an aromatic residue (W, For Y) are shown in Table 19 and the peptides which are hydrophobic withnon-aromatic sequences but have amino acids residue other than A, V, L,I, P or additional proline residues are designed (Table 20). Finally,hydrophilic and/or bending peptides which are consisted withnon-aliphatic amino acids are shown in Table 21.

TABLE 19 Proline Rigidity/ Structural Hydro- rPeptide ID Len- PositionFlexibility Feature pathy Group (SEQ ID No) Sequences gth (PP) (II) (AI)(GRAVY) Aromatic 30 (926) AMALLPAAVAVA 12 6 51.6 163.3 2.3 Peptides33 (927) AAAILAPAFLAV 12 7 57.3 171.7 2.4 (Aromatic 131 (929)WIIAPVWLAWIA 12 5 51.6 179.2 1.9 Ring 922 (929) WYVIFVLPLVVP 12 8, 1241.3 194.2 2.2 Presences) 71 (930) FMWMWFPFMWYP 12 7, 12 71.3 0.0 0.6921 (931) IWWFVVLPLVVP 12 8, 12 41.3 194.2 2.2

TABLE 20 Proline Rigidity/ Structural Hydro- rPeptide ID Len- PositionFlexibility Feature pathy Group (SEQ ID No) Sequences gth (PP) (II) (AI)(GRAVY) Hydrophobic 436 (232) VVMLVVPAVMLP 12 7, 12 57.3 194.2 2.6but Non 138 (933) PPAALLAILAVA 12 1, 2 57.3 195.8 2.2 Aromatic 77 (934)PVALVLVALVAP 12 1, 12 41.3 219.2 2.5 Peptides 577 (935) MLMIALVPMLAV 128 18.9 195.0 2.7 97 (936) ALLAAPPALLAL 12 6, 7 57.3 204.2 2.1 214 (937)ALIVAPALMALP 12 6, 12 60.5 187.5 2.2 59 (938) AVLAAPVVAALA 12 6 41.3187.5 2.5 54 (939) LAVAAPPVVALL 12 6, 7 57.3 203.3 2.3

TABLE 21 rPeptide  Proline Rigidity/ Structural ID Position FlexibiltyFeature Hydropathy Group (SEQ ID NO) Sequences Length (PP) (II)  (AI)(GRAVY Hydro- 949 (940) SGNSCQQCGNSS 12 None 41.7 0.0 −1.1 philic 39 (941) CYNTSPCTGCCY 12 6 52.5 0.0 0.0 Peptides 19 (942) YVSCCTYTNGSQ12 None 47.7 0.0 −1.0 but Non  947 (943) CYYNQQSNNNNQ 12 None 59.6 0.0−2.4 Aliphatic 139 (944) TGSTNSPTCTST 12 7 53.4 0.0 −0.7 18 (945)NYCCTPTTNGQS 12 6 47.9 0.0 −0.9 20 (946) NYCNTCPTYGQS 12 7 47.4 0.0 −0.9635 (947) GSTGGSQQNNQY 12 None 31.9 0.0 −1.9 40 (948) TYNTSCTPGTCY 12 849.4 0.0 −0.6 57 (949) QNNCNTSSQGGG 12 None 52.4 0.0 −1.6 159 (950)CYSGSTSQNQPP 12 11, 12 51.0 0.0 −1.3 700 (951) GTSNTCQSNQNS 12 None 19.10.0 −1.6 38 (952) YYNQSTCGGQCY 12 None 53.8 0.0 −1.0

3-5. Summary of Newly Designed Peptides

Total of 457 sequences have been designed based on the critical factors.Designed potentially best aMTDs (hydrophobic, flexible, bending,aliphatic and 12-A/a length peptides) that do satisfy all range/featureof critical factors are 316. Designed rPeptides that do not satisfy atleast one of the critical factors are 141 that no bending peptidesequences are 26; rigid peptide (II<40) sequences are 23; too muchflexible peptides are 24; aromatic peptides (aromatic ring presences)are 27; hydrophobic, but non-aromatic peptides are 23; and hydrophilic,but non-aliphatic peptides are 18.

4. Preparation of Recombinant Report Proteins Fused to aMTDs andrPeptides

Recombinant proteins fused to aMTDs and others [rPeptides, referencehydrophobic CPP sequences (MTM and MTD)] were expressed in a bacterialsystem, purified with single-step affinity chromatography and preparedas soluble proteins in physiological condition. These recombinantproteins have been tested for the ability of their cell-permeability byutilizing flow cytometry and laser scanning confocal microscopy.

4-1. Selection of Cargo Protein for Recombinant Proteins Fused toPeptide Sequences

For clinical/non-clinical application, aMTD-fused cargo materials wouldbe biologically active molecules that could be one of the following:enzymes, transcription factors, toxic, antigenic peptides, antibodiesand antibody fragments. Furthermore, biologically active molecules couldbe one of these following macromolecules: enzymes, hormones, carriers,immunoglobulin, membrane-bound proteins, transmembrane proteins,internal proteins, external proteins, secreted proteins, virus proteins,native proteins, glycoproteins, fragmented proteins, disulfide bondedproteins, recombinant proteins, chemically modified proteins and prions.In addition, these biologically active molecules could be one of thefollowing: nucleic acid, coding nucleic acid sequence, mRNAs, antisenseRNA molecule, carbohydrate, lipid and glycolipid.

According to these pre-required conditions, a non-functional cargo toevaluate aMTD-mediated protein uptake has been selected and called asCargo A (CRA) that should be soluble and non-functional. The domain (A/a289-840; 184 A/a length) is derived from protein S (Genbank ID:CP000113.1).

4-2. Construction of Expression Vector and Preparation of RecombinantProteins

Coding sequences for recombinant proteins fused to each aMTD are clonedNdel (5′) and SalI (3′) in pET-28a(+) (Novagen, Darmstadt, Germany) fromPCR-amplified DNA segments. PCR primers for the recombinant proteinsfused to aMTD and rPeptides are SEQ ID NOs: 481˜797. Structure of therecombinant proteins is displayed in FIG. 1.

The recombinant proteins were forcedly expressed in E. coli BL21 (DE3)cells grown to an OD₆₀₀ of 0.6 and induced for 2 hours with 0.7 mMisopropyl-β-D-thiogalactopyranoside (IPTG). The proteins were purifiedby Ni²⁺ affinity chromatography as directed by the supplier (Qiagen,Hilden, Germany) in natural condition. After the purification, purifiedproteins were dissolved in a physiological buffer such as DMEM medium.

TABLE 22

Potentially Best aMTDs (Hydrophobic, Flexible, Bending, :240 Aliphatic &Helical)

Random Peptides :31 No Bending Peptides (No Proline at 5 or 6 and/or 12):02 No Bending Peptides (No Central Proline) :01 Rigid Peptides (II <50) :09 Too Much Flexible Peptides :09 Aromatic Peptides (Aromatic RingPresences) :01 Hydrophobic, But Non-Aromatic Peptides :02 Hydrophilic,But Non-Aliphatic Peptides :07

4-3. Expression of aMTD- or Random Peptide (rP)-Fused RecombinantProteins

Using the standardized six critical factors, 316 aMTD sequences havebeen designed. In addition, 141 rPeptides are also developed that lackone of these critical factors: no bending peptides: i) absence ofproline both in the middle and at the end of sequence or ii) absence ofproline either in the middle or at the end of sequence, rigid peptides,too much flexible peptides, aromatic peptides (aromatic ring presence),hydrophobic but non-aromatic peptides, and hydrophilic but non-aliphaticpeptides (Table 22).

These rPeptides are devised to be compared and contrasted with aMTDs inorder to analyze structure/sequence activity relationship (SAR) of eachcritical factor with regard to the peptides' intracellular deliverypotential. All peptide (aMTD or rPeptide)-containing recombinantproteins have been fused to the CRA to enhance the solubility of therecombinant proteins to be expressed, purified, prepared and analyzed.

These designed 316 aMTDs and 141 rPeptides fused to CRA were all cloned(FIG. 2) and tested for inducible expression in E. coli (FIG. 3). Out ofthese peptides, 240 aMTDs were inducibly expressed, purified andprepared in soluble form (FIG. 4). In addition, 31 rPeptides were alsoprepared as soluble form (FIG. 4).

To prepare the proteins fused to rPeptides, 60 proteins were expressedthat were 10 out of 26 rPeptides in the category of no bending peptides(Table 16); 15 out of 23 in the category of rigid peptides [instabilityindex (II)<40](Table 17); 19 out of 24 in the category of too muchflexible peptides (Table 18); 6 out of 27 in the category of aromaticpeptides (Table 19); 8 out of 23 in the category of hydrophobic butnon-aromatic peptides (Table 20); and 12 out of 18 in the category ofhydrophilic but non-aliphatic peptides (Table 21).

4-4. Quantitative Cell-Permeability of aMTD-Fused Recombinant Proteins

The aMTDs and rPeptides were fluorescently labeled and compared based onthe critical factors for cell-permeability by using flow cytometry andconfocal laser scanning microscopy (FIGS. 5 to 8). The cellular uptakeof the peptide-fused non-functional cargo recombinant proteins couldquantitatively be evaluated in flow cytometry, while confocal laserscanning microscopy allows intracellular uptake to be assessed visually.The analysis included recombinant proteins fused to a negative control[rP38] that has opposite characteristics (hydrophilic and aromaticsequence: YYNQSTCGGQCY (SEQ ID NO: 1012)) to the aMTDs (hydrophobic andaliphatic sequences). Relative cell-permeability (relative fold) ofaMTDs to the negative control was also analyzed (Table 23 and FIG. 9).

Table 23 shows Comparison Analysis of Cell-Permeability of aMTDs with aNegative Control (A: rP38 (SEQ ID NO: 952)).

TABLE 23 Negative Control rP38 aMTD 19.6 ± 1.6* The Average of (Best:164.2) 240 aMTDs *Relative Fold (aMTD in Geo Mean in its comparison torP38)

Relative cell-permeability (relative fold) of aMTDs to the referenceCPPs [B: MTM12 (AAVLLPVLLAAP)(SEQ ID NO: 953), C: MTD85 (AVALLILAV) (SEQID NO: 954)] was also analyzed (Tables 40 and 41)

Table 24 shows Comparison Analysis of Cell-Permeability of aMTDs with aReference CPP (B: MTM12 (SEQ ID NO: 953)).

TABLE 24 MTM12 aMTD 13.1 ± 1.1* The Average of (Best: 109.9) 240 aMTDs*Relative Fold (aMTD in Geo Mean in its comparison to MTM12)

Table 25 shows Comparison Analysis of Cell-Permeability of aMTDs with aReference CPP (C: MTD85 (SEQ ID NO: 954)).

TABLE 25 MTD85 aMTD 6.6 ± 0.5* The Average of (Best: 55.5) 240 aMTDs*Relative Fold (aMTD in Geo Mean in its comparison to MTD85)

Geometric means of negative control (histidine-tagged rP38 (SEQ ID NO:952)-fused CRA recombinant protein) subtracted by that of naked protein(histidine-tagged CRA protein) lacking any peptide (rP38 (SEQ ID NO:952) or aMTD) was standardized as relative fold of 1. Relativecell-permeability of 240 aMTDs to the negative control (A type) wassignificantly increased by up to 164 fold, with average increase of19.6±1.6 (Table 26-31).

TABLE 26 Rigid- ity/ Sequence Proline Flexi- Stuctural Hydro- Relative ID  Position bilty Feature pathy Ration (Fold) Number aMTD SequencesLength (PP) (II) (AI) (GRAVY) A B C 229 899 AVVIALPAVVAP 12 7 57.3 195.02.4 164.2 109.9 55.5 237 908 VALALAPVVVAP 12 7 57.3 195.0 2.3 150.6100.8 50.9 238 910 VAALLPAVVVAP 12 6 57.3 195.0 2.3 148.5 99.4 50.2 185810 VIVLAAPALAAP 12 7 50.2 187.5 2.2 120.0 80.3 40.6 233 904AVLAVVAPVVAP 12 8 57.3 186.7 2.4 105.7` 70.8 35.8 74 321 IVAVALPALAVP 127 50.2 203.3 2.3 97.8 65.2 32.9 204 851 VLAVVLPAVALP 12 7 57.3 219.2 2.596.6 64.7 32.7 239 911 VALALPAVVVAP 12 6 57.3 195.0 2.3 84.8 56.8 28.7205 852 VLAVAAPAVLLP 12 7 57.3 203.3 2.3 84.6 56.6 28.6 179 803AIALAVPVLALP 12 7 57.3 211.7 2.4 74.7 50.0 25.3 222 888 ILAVVAIPAAAP 128 54.9 187.5 2.3 71.0 47.5 24.0 188 825 IVAVIVAPAVAP 12 8 43.2 195.0 2.569.7 46.6 23.6 226 895 AIIIVVPAIAAP 12 7 50.2 211.7 2.5 60.8 40.7 20.6227 896 AILIVVAPIAAP 12 8 50.2 211.7 2.5 57.5 38.5 19.4 164 727VALAIALPAVLP 12 8 57.3 211.6 2.3 54.7 36.7 18.5 139 603 VLVALAAPVIAP 128 57.3 203.3 2.4 54.1 36.1 18.2 200 847 LVAIVVLPAVAP 12 8 50.2 219.2 2.650.2 33.4 16.9 189 826 LVALAAPIIAVP 12 7 41.3 211.7 2.4 49.2 32.9 16.6161 724 VAVLAVLPALAP 12 8 57.3 203.3 2.3 47.5 31.8 16.1 131 563ALAVIVVPALAP 12 8 50.2 203.3 2.4 47.1 31.4 15.9 186 811 AVVLAVPALAVP 127 57.3 195.0 2.3 46.5 31.1 15.7 194 831 IIVAVAPAAIVP 12 7 43.2 203.3 2.546.3 31.0 15.7 192 829 AALALVAPVIVP 12 8 50.2 203.3 2.4 44.8 30.0 15.2224 891 ILAVAAIPAALP 12 8 54.9 195.8 2.2 44.7 29.9 15.1 234 905AVIAVAPLVVAP 12 7 41.3 195.0 2.4 44.0 29.5 14.9 132 564 VAIALIVPALAP 128 50.2 211.7 2.4 43.6 29.1 14.7 34 124 IAVALPALIAAP 12 6 50.3 195.8 2.243.6 29.0 14.7 190 827 IAAVLAAPALVP 12 8 57.3 187.5 2.2 43.0 28.8 14.6 22 AAAVPLLAVVVP 12 5 41.3 195.0 2.4 40.9 27.2 13.8 91 385 IVAIAVPALVAP 127 50.2 203.3 2.4 38.8 25.9 13.1 191 828 IALLAAPIIAVP 12 7 41.3 220.0 2.436.8 24.6 12.4 181 806 LVALAVPAAVLP 12 7 57.3 203.3 2.3 36.7 24.6 12.4198 845 AAVVIAPLLAVP 12 7 41.3 203.3 2.4 35.8 24.0 12.1 218 882AIALVVPAVAVP 12 7 57.3 195.0 2.4 35.0 23.4 11.8 128 545 VVLVLAAPAAVP 128 57.3 195.0 2.3 34.6 23.1 11.7 39 161 AVIALPALIAAP 12 6 57.3 195.8 2.234.5 23.0 11.6 110 481 AIAIAIVPVALP 12 8 50.2 211.6 2.4 34.3 23.0 11.6230 900 ALVAVIAPVVAP 12 8 57.3 195.0 2.4 34.3 22.9 11.6 53 223AILAVPIAVVAP 12 6 57.3 203.3 2.4 33.0 22.1 11.2 187 824 LIIVAAAPAVAP 128 50.2 187.5 2.3 32.8 21.9 11.1 130 562 ALIAAIVPALVP 12 8 50.2 211.7 2.432.7 21.8 11.0 52 222 ALLIAPAAVIAP 12 6 57.3 195.8 2.2 32.6 21.7 11.0 1761 VAALPVLLAALP 12 5 57.3 211.7 2.3 31.2 20.8 10.5 134 582 VAVALIVPALAP12 8 50.2 203.3 2.4 30.6 20.4 10.3 223 889 ILVAAAPIAALP 12 7 57.3 195.82.2 30.3 20.3 10.3 177 787 AVALVPVIVAAP 12 6 50.2 195.0 2.4 29.3 19.69.9 157 703 IVAVALVPALAP 12 8 50.2 203.3 2.4 29.2 19.5 9.9 158 705IVAVALLPALAP 12 8 50.2 211.7 2.4 28.6 19.1 9.7 220 885 LVAIAPAVAVLP 12 657.3 203.3 2.4 28.3 19.0 9.6 3 3 AALLVPAAVLAP 12 6 57.3 187.5 2.1 27.018.0 9.1 137 601 AAILIAVPIAAP 12 8 57.3 195.8 2.3 26.8 17.9 9.0 196 843AVLVLVAPAAAP 12 8 41.3 219.2 2.5 26.4 17.7 8.9 94 403 AAALVIPAAILP 12 754.9 195.8 2.2 25.2 16.8 8.5 127 544 IVALIVAPAAVP 12 8 43.1 203.3 2.423.4 15.6 7.9 121 522 ALLVIAVPAVAP 12 8 57.3 203.3 2.4 22.7 15.2 7.7

TABLE 27 Rigid- ity/ Sequence Proline Flexi- Stuctural Hydro- Relative ID  Position bilty Feature pathy Ration (Fold) Number aMTD SequencesLength (PP) (II) (AI) (GRAVY) A B C 180 805 LVLIAAAPIALP 12 8 41.3 220.02.4 22.3 14.9 7.6 108 464 AVVILVPLAAAP 12 7 57.3 203.3 2.4 22.3 14.9 7.596 405 LAAAVIPVAILP 12 7 54.9 211.7 2.4 22.2 14.8 7.5 168 747VALLAIAPALAP 12 8 57.3 195.8 2.2 22.0 14.8 7.5 115 501 VIALAVPALAP 12 850.2 203.3 2.4 21.5 14.4 7.3 147 661 AAILAPIVAALP 12 6 50.2 195.8 2.221.4 14.3 7.2 176 786 LVAIAPLAVLAP 12 6 41.3 211.7 2.4 21.2 14.2 7.2 144625 ILAAAAAPLIVP 12 8 50.2 195.8 2.2 20.9 13.9 7.0 101 442 ALAALVPAVLVP12 7 57.3 203.3 2.3 20.4 13.6 6.9 240 912 VALLAPAVVVAP 12 6 57.3 195.02.3 19.9 13.3 6.7 43 165 ALAVPVALAIVP 12 5 50.2 203.3 2.4 19.8 13.2 6.798 422 VVAILAPLLAAP 12 7 57.3 211.7 2.4 19.6 13.1 6.6 155 686AALVAVLPVALP 12 8 57.3 203.3 2.3 19.5 13.1 6.6 81 343 IVAVALPALVAP 12 750.2 203.3 2.3 19.4 12.9 6.5 76 323 IVAVALPVALAP 12 7 50.2 203.3 2.319.1 12.8 6.4 105 461 IAAVIVPAVALP 12 7 50.2 203.3 2.4 19.0 12.7 6.4 921 AVALLPALLAVP 12 6 57.3 211.7 2.3 18.9 12.6 6.4 95 404 LAAAVIPAAILP 127 54.9 195.8 2.2 18.9 12.6 6.4 60 261 LVLVPLLAAAAP 12 5 41.3 511.6 2.318.5 12.3 6.2 122 524 AVALIVVPALAP 12 8 50.2 203.3 2.4 18.3 12.2 6.2 55225 VAALLPAAAVLP 12 6 57.3 187.5 2.1 18.3 12.2 6.2 63 264 LAAAPVVIVIAP12 5 50.2 203.3 2.4 18.2 12.1 6.1 1 1 AAALAPVVLALP 12 6 57.3 187.5 2.117.7 11.8 6.0 88 382 AAALVIPAILAP 12 7 54.9 195.8 2.2 17.7 11.8 6.0 107463 AVAILVPLLAAP 12 7 57.3 211.7 2.4 17.6 11.7 5.9 75 322 VVAIVLPAIAAP12 7 50.2 203.3 2.3 17.6 11.7 5.9 117 503 AAIIIVLPAALP 12 8 50.2 220.02.4 17.6 11.8 5.9 211 870 VLVAAVLPIAAP 12 8 41.3 203.3 2.4 16.6 11.1 5.656 241 AAAVVPVLLVAP 12 6 57.3 195.0 2.4 16.6 11.0 5.6 163 726LAVAIIAPAVAP 12 8 57.3 187.5 2.2 16.5 11.0 5.6 79 341 IVAVALPAVLAP 12 750.2 203.3 2.3 16.4 10.9 5.5 125 542 ALALIIVPAVAP 12 8 50.2 211.6 2.416.2 10.8 5.5 83 361 AVVIVAPAVIAP 12 7 50.2 195.0 2.4 16.0 10.7 5.4 54224 ILAAVPIALAAP 12 6 57.3 195.8 2.2 15.8 10.6 5.3 20 64 AIVALPVAVLAP 126 50.2 203.3 2.4 15.8 10.6 5.3 111 482 ILAVAAIPVAVP 12 8 54.9 203.3 2.415.8 10.6 5.3 113 484 LAVVLAAPAIVP 12 8 50.2 203.3 2.4 15.6 10.4 5.3 210868 VLVAAILPAAIP 12 8 54.9 211.7 2.4 14.9 10.0 5.0 124 541 LLALIIAPAAAP12 8 57.3 204.1 2.1 14.8 9.9 5.0 150 666 AAIAIIAPAIVP 12 8 50.2 195.82.3 14.7 9.9 5.0 149 665 LAIVLAAPVAVP 12 8 50.2 203.3 2.3 14.7 9.9 5.084 363 AVLAVAPALIVP 12 7 50.2 203.3 2.3 14.7 9.8 4.9 57 242 AALLVPALVAAP12 6 57.3 187.5 2.1 14.6 9.7 4.9 90 384 VIVAIAPALLAP 12 7 50.2 211.6 2.414.0 9.4 4.7 214 877 VAIIAVPAVVAP 12 7 57.3 195.0 2.4 14.0 9.4 4.7 206863 AAVVLLPIIAAP 12 7 41.3 211.7 2.4 13.8 9.3 4.7 123 525 ALAIVVAPVAVP12 8 50.2 195.0 2.4 13.8 9.2 4.7 213 875 AIAIVVPAVAVP 12 7 50.2 195.02.4 13.8 9.2 4.7 69 285 AIVLLPAAVVAP 12 6 50.2 203.3 2.4 13.3 8.9 4.5 65281 ALIVLPAAVAVP 12 6 50.2 203.3 2.4 13.3 8.9 4.5 209 867 ALLVVIAPLAAP12 8 41.3 211.7 2.4 13.2 8.8 4.4 172 766 IVVIAVAPAVAP 12 8 50.2 195.02.4 12.9 8.6 4.4 80 342 VIVALAPAVLAP 12 7 50.2 203.3 2.3 12.7 8.5 4.3217 881 AALIVVPAVAVP 12 7 50.2 195.0 2.4 12.7 8.5 4.3 119 505AIIIVIAPAAAP 12 8 50.2 195.8 2.3 12.4 8.3 4.2

TABLE 28 Rigid- ity/ Sequence Proline Flexi- Stuctural Hydro- Relative ID  Position bilty Feature pathy Ration (Fold) Number aMTD SequencesLength (PP) (II) (AI) (GRAVY) A B C 169 763 VAVLIAVPALAP 12 8 57.3 203.32.3 12.3 7.2 4.2 159 706 IVAVALLPAVAP 12 8 50.2 203.3 2.4 12.0 7.0 4.1156 687 AILAVALPLLAP 12 8 57.3 220.0 2.3 12.0 7.0 4.1 145 643LALVLAAPAIVP 12 8 50.2 211.6 2.4 11.8 7.9 4.0 66 282 VLAVAPALIVAP 12 650.2 203.3 2.4 11.8 7.9 4.0 126 543 LLAALIAPAALP 12 8 57.3 204.1 2.111.7 7.8 4.0 78 325 IVAVALPAVALP 12 7 50.2 203.3 2.3 11.7 7.8 4.0 199846 IAVAVAAPLLVP 12 8 41.3 203.3 2.4 11.7 6.8 4.0 89 383 VIVALAPALLAP 127 50.2 211.6 2.3 11.6 7.7 3.9 87 381 VVAIVLPAVAAP 12 7 50.2 195.0 2.411.5 7.7 3.9 183 808 LVVLAAAPLAVP 12 8 41.3 203.3 2.3 11.5 7.6 3.9 208865 AVLVIAVPAIAP 12 8 57.3 203.3 2.5 11.3 7.5 3.8 162 725 IAVLAVAPAVLP12 8 57.3 203.3 2.3 11.2 7.5 3.8 197 844 VVALLAPLIAAP 12 7 41.3 211.82.4 11.2 7.5 3.8 228 897 AVIVPVAIIAAP 12 5 50.2 203.3 2.5 11.2 7.5 3.8141 605 VIAAVLAPVAVP 12 8 57.3 195.0 2.4 11.0 7.4 3.7 166 744AAVVIVAPVALP 12 8 50.2 195.0 2.4 11.0 7.3 3.7 51 221 AAILAPIVALAP 12 650.2 195.8 2.2 10.9 7.3 3.7 142 622 ALIVLAAPVAVP 12 8 50.2 203.3 2.410.6 7.1 3.6 92 401 AALAVIPAAILP 12 7 54.9 195.8 2.2 10.6 7.1 6.6 77 324IVAVALPAALVP 12 7 50.2 203.3 2.3 10.3 6.9 3.5 215 878 IVALVAPAAVVP 12 750.2 195.0 2.4 10.3 6.9 6.5 71 302 LALAPALALLAP 12 5 57.3 204.2 2.1 10.26.8 3.4 154 685 ALLVAVLPAALP 12 8 57.3 211.7 2.3 10.2 5.9 3.4 201 848AVAIVVLPAVAP 12 8 50.2 195.0 2.4 10.0 6.7 3.4 1338 602 VIVALAAPVLAP 12 850.2 203.3 2.4 9.9 5.8 3.4 178 788 AIAVAIAPVALP 12 8 57.3 187.5 2.3 9.86.6 3.3 38 145 LLAVVPAVALAP 12 6 57.3 203.3 2.3 9.5 6.3 3.2 6 11VVALAPALAALP 12 6 57.3 187.5 2.1 9.5 6.3 3.2 35 141 AVIVLPALAVAP 12 650.2 203.3 2.4 9.4 6.3 3.2 120 521 LAALIVVPAVAP 12 8 50.2 203.3 2.4 9.46.3 3.2 100 425 AVVAIAPVLALP 12 7 57.3 203.3 2.4 9.4 6.3 3.2 86 365AVIVVAPALLAP 12 7 50.2 203.3 2.3 9.3 6.2 3.1 62 263 ALAVIPAAAILP 12 654.9 195.8 2.2 9.0 6.0 3.0 82 345 ALLIVAPVAVAP 12 7 50.2 203.3 2.3 8.95.9 3.0 203 850 LVIALAAPVALP 12 8 57.3 211.7 2.4 8.8 5.9 3.0 37 144VLAIVPAVALAP 12 6 50.2 203.3 2.4 8.8 5.9 3.0 173 767 IVVAAVVPALAP 12 850.2 195.0 2.4 8.5 5.0 2.9 47 185 AALVLPLIIAAP 12 6 41.3 220.0 2.4 8.55.7 2.9 202 849 AVILLAPLIAAP 12 7 57.3 220.0 2.4 8.3 4.8 2.8 207 864ALLVIAPAIAVP 12 7 57.3 211.7 2.4 8.2 4.8 2.8 40 162 AVVALPAALIVP 12 650.2 203.3 2.4 8.2 5.5 2.8 42 164 LAAVLPALLAAP 12 6 57.3 195.8 2.1 8.25.5 2.8 236 907 VAIALAPVVVAP 12 7 57.3 195.0 2.4 8.1 5.4 2.8 103 444LAAALVPVALVP 12 7 57.3 203.3 2.3 8.1 5.4 2.7 102 443 ALAALVPVALVP 12 757.3 203.3 2.3 8.0 5.3 2.7 231 901 ALVAVLPAVAVP 12 7 57.3 195.0 2.4 7.75.1 2.6 221 887 VLAVAPAVAVLP 12 6 57.3 195.0 2.4 7.7 5.1 2.6 167 746VAIIVVAPALAP 12 8 50.2 203.3 2.4 7.6 4.4 2.6 232 902 ALVAPLLAVAVP 12 541.3 203.3 2.3 7.6 5.1 2.6 133 565 VAIVLVAPAVAP 12 8 50.2 195.0 2.4 7.55.0 2.5 59 245 AAALAPVLALVP 12 6 57.3 187.5 2.1 7.5 5.0 2.5 165 743AIAIALVPVALP 12 8 57.3 211.6 2.4 7.4 4.9 2.5 109 465 AVVILVPLAAAP 12 757.6 203.3 2.4 7.4 4.9 2.5 30 104 AVVAAPLVLALP 12 6 41.3 203.3 2.3 7.34.9 2.5

TABLE 29 Rigid- ity/ Sequence Proline Flexi- Stuctural Hydro- Relative ID  Position bilty Feature pathy Ration (Fold) Number aMTD SequencesLength (PP) (II) (AI) (GRAVY) A B C 160 707 IVALAVLPAVAP 12 8 50.2 203.32.4 7.3 4.9 2.5 212 872 VLAAAVLPLVVP 12 8 41.3 219.2 2.5 7.3 4.9 2.5 135583 AVILALAPIVAP 12 8 50.2 211.6 2.4 7.3 4.8 2.4 216 879 AAIVLLPAVVVP 127 50.2 219.1 2.5 7.2 4.8 2.4 175 784 VAALPAVALVVP 12 5 57.3 195.0 2.47.1 4.7 2.4 225 893 VIAIPAILAAAP 12 5 54.9 195.8 2.3 7.0 4.7 2.4 8 13AAALVPVVALLP 12 6 57.3 203.3 2.3 7.0 4.7 2.4 184 809 LIVLAAPALAAP 12 750.2 195.8 2.2 7.0 4.7 2.4 104 445 ALAALVPALVVP 12 7 57.3 203.3 2.3 6.94.6 2.3 22 81 AALLPALAALLP 12 5 57.3 204.2 2.1 6.9 4.6 2.3 151 667LAVAIVAPALVP 12 8 50.2 203.3 2.3 6.9 4.6 2.3 235 906 AVIALAPVVVAP 12 757.3 195.0 2.4 6.8 4.6 2.3 112 483 ILAAAIIPAALP 12 8 54.9 204.1 2.2 6.84.5 2.3 114 485 AILAAIVPLAVP 12 8 50.2 211.6 2.4 6.8 4.5 2.3 97 421AAILAAPLIAVP 12 7 57.3 195.8 2.2 6.7 4.5 2.3 136 585 ALIVAIAPALVP 12 850.2 211.6 2.4 6.6 4.4 2.2 99 424 AVVVAAPVLAIP 12 7 57.3 195.0 2.4 6.64.4 2.2 85 364 LVAAVAPALIVP 12 7 50.2 203.3 2.3 6.5 4.3 2.2 93 402ALAAVIPAAILP 12 7 54.9 195.8 2.2 6.4 4.3 2.2 106 462 IAAVLVPAVALP 12 757.3 20.3 2.4 6.3 4.2 2.1 64 265 VLAIAPLLAAVP 12 6 41.3 211.6 2.3 6.04.0 2.0 70 301 VIAAPVLAVLAP 12 6 57.3 203.3 2.4 6.0 4.0 2.0 45 183LLAAPVVIALAP 12 6 57.3 211.6 2.4 6.0 4.0 2.0 58 243 AAVLLPVALAAP 12 657.3 187.5 2.1 5.9 3.9 2.0 148 664 ILIAIAIPAAAP 12 8 54.9 204.1 2.3 5.73.8 1.9 174 783 IVALVPAVAIAP 12 6 50.2 203.3 2.5 5.7 3.8 1.9 116 502AIVALAVPVLAP 12 8 50.2 203.3 2.4 5.6 3.7 1.9 61 262 ALIAVPAIIVAP 12 650.2 211.6 2.4 5.5 3.7 1.9 152 683 LAIVLAAPAVLP 12 8 50.2 211.7 2.4 5.53.2 1.9 193 830 IALVAAPVALVP 12 7 57.3 203.3 2.4 5.3 3.5 1.8 170 764AVALAVLPAVVP 12 8 57.3 195.0 2.3 5.0 3.4 1.7 182 807 AVALAVLPAVVP 12 757.3 203.3 2.3 5.0 3.3 1.7 46 184 LAAIVPAIIAVP 12 6 50.2 211.6 2.4 4.83.2 1.6 73 305 IALAAPILLAAP 12 6 57.3 204.2 2.2 4.8 3.2 1.6 27 101LVALAPVAAVLP 12 6 57.3 203.3 2.3 4.5 3.0 1.5 72 304 AIILAPIAAIAP 12 657.3 204.2 2.3 4.4 3.0 1.5 140 604 VALIAVAPAVVP 12 8 57.3 195.0 2.4 4.32.5 1.5 146 645 ALAVVALPAIVP 12 8 50.2 203.3 2.4 4.3 2.9 1.5 48 201LALAVPALAALP 12 6 57.3 195.8 2.1 4.2 2.8 1.4 41 163 LALVLPAALAAP 12 657.3 195.8 2.1 4.1 2.4 1.4 195 832 AVAAIVPVIVAP 12 7 43.2 195.0 2.5 4.12.7 1.4 44 182 ALIAPVVALVAP 12 6 57.3 203.3 2.4 4.0 2.7 1.4 11 23VVLVLPAAAAVP 12 6 57.3 195.0 2.4 4.0 2.6 1.3 31 105 LLALAPAALLAP 12 657.3 204.1 2.1 4.0 2.6 1.3 129 561 AAVAIVLPAVVP 12 8 50.2 195.0 2.4 3.92.6 1.3 171 765 AVALAVVPAVLP 12 8 57.3 195.0 2.3 3.8 2.2 1.3 153 684AAIVLALPAVLP 12 8 50.2 211.7 2.4 3.5 2.1 1.2 36 143 AVLAVPAVLVAP 12 657.3 195.0 2.4 3.3 2.2 1.1 118 504 LIVALAVPALAP 12 8 50.2 211.7 2.4 3.32.2 1.1 10 22 AVVLVPVLAAAP 12 6 57.3 195.0 2.4 3.1 2.1 1.1 5 5AAALLPVALVAP 12 6 57.3 187.5 2.1 3.1 2.1 1.0 67 283 AALLAPALIVAP 12 650.2 195.8 2.2 3.1 2.0 1.0 21 65 IAIVAPVVALAP 12 6 50.2 203.3 2.4 3.02.0 1.0 219 883 LAIVPAAIAALP 12 6 50.2 195.8 2.2 3.0 2.0 1.0 33 123AAIIVPAALLAP 12 6 50.2 195.8 2.2 3.0 2.0 1.0

TABLE 30 Rigid- ity/ Sequence Proline Flexi- Stuctural Hydro- Relative ID  Position bilty Feature pathy Ration (Fold) Number aMTD SequencesLength (PP) (II) (AI) (GRAVY) A B C 68 284 ALLAPAVALIVP 12 5 50.2 211.72.4 2.8 1.8 0.9 50 205 ALALVPAIAALP 12 6 57.3 195.8 2.2 2.6 1.7 0.9 1442 VAALPVVAVVAP 12 5 57.3 186.7 2.4 2.5 1.7 0.8 32 121 AIVALPALALAP 12 650.2 195.8 2.2 2.5 1.7 0.8 13 25 IVAVAPALVALP 12 6 50.2 203.3 2.4 2.41.6 0.8 12 24 IALAAPALIVAP 12 6 50.2 195.8 2.2 2.3 1.6 0.8 49 204LIAALPAVAALP 12 6 57.3 195.8 2.2 2.2 1.5 0.8 7 12 LLAAVPAVLLAP 12 6 57.3211.7 2.3 2.2 1.5 0.7 15 43 LLAAPLVVAAVP 12 5 41.3 187.5 2.1 2.1 1.4 0.729 103 ALALLPVAALAP 12 6 57.3 204.2 2.2 2.1 1.4 0.7 23 82 AVVLAPVAAVLP12 6 57.3 195.0 2.4 2.1 1.4 0.7 4 4 ALALLPVAALAP 12 6 57.3 195.8 2.1 2.01.3 0.7 26 85 LLVLPAAALAAP 12 5 57.3 195.8 2.1 1.9 1.3 0.7 19 63AALLVPALVAVP 12 6 57.3 203.3 2.3 1.9 1.3 0.7 16 44 ALAVPVALLVAP 12 557.3 203.3 2.3 1.6 1.1 0.5 25 84 AAVAAPLLLALP 12 6 41.3 195.8 2.1 1.51.0 0.5 18 62 VALLAPVALAVP 12 6 57.3 203.3 2.3 1.4 0.9 0.5 24 83LAVAAPLALALP 12 6 41.3 195.8 2.1 1.4 0.9 0.5 28 102 LALAPAALALLP 12 557.3 204.2 2.1 1.4 0.9 0.5 143 623 VAAAIALPAIVP 12 8 50.2 187.5 2.3 0.80.6 0.3 19.6 ± 13.1 ±  6.5 ±  1.6 1.1 0.5

Moreover, compared to reference CPPs (B type: MTM12 (SEQ ID NO: 953) andC type: MTD85 (SEQ ID NO: 954)), novel 240 aMTDs averaged of 13±1.1(maximum 109.9) and 6.6±0.5 (maximum 55.5) fold highercell-permeability, respectively (Tables 26-31).

TABLE 31 Negative Control rP38 MTM12 MTD85 aMTD 19.6 ± 1.6* 13.1 ± 1.1*6.6 ± 0.5* The Average, of 240 aMTDs (Best 164.2) (Best 109.9) (Best:55.5) *Relative Fold (aMTD in Geo Mean in its comparison to rP38, MTM12or MTD85)

In addition, cell-permeability of 31 rPeptides has been compared withthat of 240 aMTDs (0.3±0.04; Tables 32 and 33).

TABLE 32 Rigid- ity/ Relative Number Proline Flexi- Stuctural Hydro-Ratio (SEQ ID Position bilty Feature pathy To aMTD NOS) ID SequenceLength (PP) (II) (AI) (GRAVY) AVE 907 692 PAPLPPVVILAV 12 1,3,5,6 105.5186.7 1.8 0.74 895 26 AAIAKLAAPLAIV 12 8 18.1 204.2 2.5 0.65 891 113PVAVALLIAVPP 12 1,11,12 57.3 195.0 2.1 0.61 897 466 IIAAAAPLAIIP 12 7,1222.8 204.2 2.3 0.52 898 167 VAIAIPAALAIP 12 6,12 20.4 195.8 2.3 0.50 93697 ALLAAPPALLAL 12 6,7 57.3 204.2 2.1 0.41 909 390 VPLLVPVVPVVP 122,6,9,12 105.4 210.0 2.2 0.41 900 426 AAALAIPLAIIP 12 7,12 4.37 204.22.2 0.40 937 241 ALIVAPALMALP 12 6,12 60.5 187.5 2.2 0.33 913 68VAPVLPAAPLVP 12 3,6,9,12 105.5 162.5 1.6 0.32 941 39 CYNTSPCTGCCY 12 652.5 0.0 0.0 0.29 888 934 LILAPAAVVAAA 12 5 57.5 195.8 2.5 0.28 916 938VPVLLPVVVPVP 12 2,6,10,12 121.5 210.0 2.2 0.28 917 329 VPVLVPVVPVVP 122,6,9,12 121.5 210.0 2.2 0.23 901 606 AAAIAAIPIIIP 12 8,12 4.4 204.2 2.40.20 918 49 VVPAAPAVPVVP 12 3,6,9,12 121.5 145.8 1.7 0.18 944 139TGSTNSPTCTST 12 7 53.4 0.0 −0.7 0.17 919 772 LPVAPVIPIIVP 12 2,5,8,1279.9 210.8 2.1 0.16 931 921 IWWFVVLPLVVP 12 8,12 41.3 194.2 2.2 0.14 90266 AGVLGGPIMGVP 12 7,12 35.5 121.7 1.3 0.13 922 693 AAPVLPVAVPIV 123,6,10 82.3 186.7 2.1 0.13 945 18 NYCCTPTTNGQS 12 6 47.9 0.0 −0.9 0.10890 16 NNSCTTYTNGSQ 12 None 47.4 0.0 −1.4 0.08 904 227 LAAIVPIAAAVP 126,12 34.2 187.5 2.2 0.08 905 17 GGCSAPQTTCSN 12 6 51.6 8.3 −0.5 0.08 90667 LDAEVPLADDVP 12 6,12 34.2 130.0 0.3 0.08 947 635 GSTGGSQQNNQY 12 None31.9 0.0 −1.9 0.07 924 29 VLPPLPVLPVLP 12 3,4,6,9,12 121.5 202.5 1.70.07 949 57 QNNCNTSSQGGG 12 None 52.4 0.0 −1.6 0.06 951 700 GTSNTCQSNQNS12 None 19.1 0.0 −1.6 0.05 952 38 YYNQSTCGGQCY 12 ND 53.8 0.0 −1.0 0.05AVE 0.3 ± 0.04

TABLE 33 Relative Ratio to aMTD AVE* rPeptide 0.3 ± 0.04 The Average of31 aMTDs *Out of 240 aMTDs, average relative fold of aMTD had been 19.6fold compared to type A (rP38).

In summary, relative cell-permeability of aMTDs has shown maximum of164.0, 109.9 and 55.5 fold higher to rP38 (SEQ ID NO: 952), MTM12 (SEQID NO: 953) and MTD85 (SEQ ID NO: 954), respectively. In average oftotal 240 aMTD sequences, 19.6±1.6, 13.1±1.1 and 6.6±0.5 fold highercell-permeability are shown to the rP38 (SEQ ID NO: 952), MTM12 (SEQ IDNO: 953) and MTD85 (SEQ ID NO: 954), respectively (Tables 26-31).Relative cell-permeability of negative control (rP38 (SEQ ID NO: 952))to the 240 aMTDs is only 0.3±0.04 fold.

4-5. Intracellular Delivery and Localization of aMTD-Fused RecombinantProteins

Recombinant proteins fused to the aMTDs were tested to determine theirintracellular delivery and localization by laser scanning confocalmicroscopy with a negative control (rP38 (SEQ ID NO: 952)) and previouspublished CPPs (MTM12 (SEQ ID NO: 953) and MTD85 (SEQ ID NO: 954)) asthe positive control references. NIH3T3 cells were exposed to 10 μM ofFITC-labeled protein for 1 hour at 37, and nuclei were counterstainedwith DAPI. Then, cells were examined by confocal laser scanningmicroscopy (FIG. 7). Recombinant proteins fused to aMTDs clearly displayintracellular delivery and cytoplasmic localization (FIG. 7) that aretypically higher than the reference CPPs (MTM12 (SEQ ID NO: 953) andMTD85 (SEQ ID NO: 954)). The rP38 (SEQ ID NO: 952)-fused recombinantprotein did not show internalized fluorescence signal (FIG. 7a ). Inaddition, as seen in FIG. 8, rPeptides (his-tagged CRA recombinantproteins fused to each rPeptide) display lower- ornon-cell-permeability.

4-6. Summary of Quantitative and Visual Cell-Permeability of NewlyDeveloped aMTDS

Histidine-tagged aMTD-fused cargo recombinant proteins have been greatlyenhanced in their solubility and yield. Thus, FITC-conjugatedrecombinant proteins have also been tested to quantitate and visualizeintracellular localization of the proteins and demonstrated highercell-permeability compared to the reference CPPs.

In the previous studies using the hydrophobic signal-sequence-derivedCPPs-MTS/MTM or MTDs, 17 published sequences have been identified andanalyzed in various characteristics such as length, molecular weight, pIvalue, bending potential, rigidity, flexibility, structural feature,hydropathy, amino acid residue and composition, and secondary structureof the peptides. Based on these analytical data of the sequences, novelartificial and non-natural peptide sequences designated as advanced MTDs(aMTDs) have been invented and determined their functional activity inintracellular delivery potential with aMTD-fused recombinant proteins.

aMTD-fused recombinant proteins have promoted the ability of proteintransduction into the cells compared to the recombinant proteinscontaining rPeptides and/or reference hydrophobic CPPs (MTM12 (SEQ IDNO: 953) and MTD85 (SEQ ID NO: 954)). According to the results, it hasbeen demonstrated that critical factors of cell-penetrating peptidesequences play a major role to determine peptide-mediated intracellulardelivery by penetrating plasma membrane. In addition, cell-permeabilitycan considerably be improved by following the rational that all satisfythe critical factors.

5. Structure/Sequence Activity Relationship (SAR) of aMTDs on DeliveryPotential

After determining the cell-permeability of novel aMTDs,structure/sequence activity relationship (SAR) has been analyzed foreach critical factor in selected some of and all of novel aMTDs (FIGS.13 to 16 and Table 34).

TABLE 34 Rank of Rigidity/ Sturcture Relative Amino Delivery FlexibilityFeature Hydropethy Ratio (Fold) Acid Composition Potential (II) (AI)(GRAVY) A B C A V I L  1~10 55.9 199.2 2.3 112.7 75.5 38.1 4.0 3.5 0.42.1  11~20 51.2 205.8 2.4 58.2 37.6 19.0 4.0 2.7 1.7 1.6  21~30 49.1199.2 2.3 43.6 28.9 14.6 4.3 2.7 1.4 1.6  31~40 52.7 201.0 2.4 34.8 23.311.8 4.2 2.7 1.5 1.6  41~50 53.8 201.9 2.3 30.0 20.0 10.1 4.3 2.3 1.12.3  51~60 51.5 205.2 2.4 23.5 15.7 7.9 4.4 2.1 1.5 2.0 222~231 52.2197.2 2.3 2.2 1.5 0.8 4.5 2.1 1.0 2.4 232~241 54.1 199.7 2.2 1.7 1.2 0.64.6 1.7 0.2 3.5

5-1. Proline Position:

In regards to the bending potential (proline position: PP), aMTDs withits proline at 7′ or 8′ amino acid in their sequences have much highercell-permeability compared to the sequences in which their prolineposition is at 5′ or 6′ (FIGS. 14a and 15a ).

5-2. Hydropathy:

In addition, when the aMTDs have GRAVY (Grand Average of Hydropathy)ranging in 2.1-2.2, these sequences display relatively lowercell-permeability, while the aMTDs with 2.3-2.6 GRAVY are shownsignificantly higher one (FIGS. 14b and 15b ).

5-3. rPeptide SAR:

To the SAR of aMTDs, rPeptides have shown similar SAR correlations inthe cell-permeability, pertaining to their proline position (PP) andhydropathy (GRAVY). These results confirms that rPeptides with highGRAVY (2.4˜2.6) have better cell-permeability (FIG. 16).

5-4. Analysis of Amino Acid Composition:

In addition to proline position and hydropathy, the difference of aminoacid composition is also analyzed. Since aMTDs are designed based oncritical factors, each aMTD-fused recombinant protein has equally twoproline sequences in the composition. Other hydrophobic and aliphaticamino acids—alanine, isoleucine, leucine and valine—are combined to formthe rest of aMTD peptide sequences.

Alanine: In the composition of amino acids, the result does not show asignificant difference by the number of alanine in terms of the aMTD'sdelivery potential because all of the aMTDs have three to five alanines.In the sequences, however, four alanine compositions show the mosteffective delivery potential (geometric mean) (FIG. 13a ).

Leucine and Isoleucine: Also, the compositions of isoleucine and leucinein the aMTD sequences show inverse relationship between the number ofamino acid (I and L) and delivery potential of aMTDs. Lower number ofisoleucine and leucine in the sequences tends to have higher deliverypotential (geometric mean) (FIGS. 13a and 13b ).

Valine: Conversely, the composition of valine of aMTD sequences showspositive correlation with their cell-permeability. When the number ofvaline in the sequence is low, the delivery potential of aMTD is alsorelatively low (FIG. 13b ).

Ten aMTDs having the highest cell-permeability are selected (averagegeometric mean: 2584±126). Their average number of valine in thesequences is 3.5; 10 aMTDs having relatively low cell-permeability(average geometric mean: 80±4) had average of 1.9 valine amino acids.The average number of valine in the sequences is lowered as theircell-permeability is also lowered as shown in FIG. 13b . Compared tohigher cell-permeable aMTDs group, lower sequences had average of 1.9 intheir valine composition. Therefore, to obtain high cell-permeablesequence, an average of 2-4 valines should be composed in the sequence.

5-5. Conclusion of SAR Analysis:

As seen in FIG. 15, all 240 aMTDs have been examined for theseassociation of the cell-permeability and the critical factors: bendingpotential (PP), rigidity/flexibility (II), structure feature (AI), andhydropathy (GRAVY), amino acid length and composition. Through thisanalysis, cell-permeability of aMTDs tends to be lower when theircentral proline position is at 5′ or 6′ and GRAVY is 2.1 or lower (FIG.15). Moreover, after investigating 10 higher and 10 lower cell-permeableaMTDs, these trends are clearly shown to confirm the association ofcell-permeability with the central proline position and hydropathy.

6. Experimental Confirmation of Index Range/Feature of Critical Factors

The range and feature of five out of six critical factors have beenempirically and experimentally determined that are also included in theindex range and feature of the critical factors initially proposedbefore conducting the experiments and SAR analysis. In terms of indexrange and feature of critical factors of newly developed 240 aMTDs, thebending potential (proline position: PP), rigidity/flexibility(Instability Index: II), structural feature (Aliphatic Index: AI),hydropathy (GRAVY), amino acid length and composition are all within thecharacteristics of the critical factors derived from analysis ofreference hydrophobic CPPs.

Therefore, our hypothesis to design and develop new hydrophobic CPPsequences as advanced MTDs is empirically and experimentally proved anddemonstrated that critical factor-based new aMTD rational design iscorrect.

TABLE 35 Summarized Critical Factors of aMTD Analysis of Newly DesignedCPPs Experimental Results Critical Factor Range Range Bending PotentialProline presences in the Proline presences in the (Proline Position: PP)middle (5′, 6′, 7′ or 8′) middle (5′, 6′, 7′ or 8′) and at the end ofand at the end of peptides peptides Rigidity/Flexibility 40-60 41.3-57.3(Instability Index: II) Structural Feature 180-220 187.5-220.0(Aliphatic Index: AI) Hydropathy 2.1-2.6  12-2.6 (Grand Average ofHydropathy GRAVY) Length  9-13 12 (Number of Amino Acid) Amino acidComposition A, V, I, L, P A, V, I, L, P7. Discovery and Development of Protein-Based New Biotherapeutics withMITT Enabled by aMTDs for Protein Therapy

Total of 240 aMTD sequences have been designed and developed based onthe critical factors. Quantitative and visual cell-permeability of 240aMTDs (hydrophobic, flexible, bending, aliphatic and 12 a/a-lengthpeptides) are all practically determined.

To measure the cell-permeability of aMTDs, rPeptides have also beendesigned and tested. As seen in FIGS. 13 to 15, there are vividassociation of cell-permeability and the critical factors of thepeptides. Out of these critical factors, we are able to configure thatthe most effective cell-permeable aMTDs have the amino acid length of12; composition of A, V, L, I and P; multiple proline located at either7′ or 8′ and at the end (12′); instability index ranged of 41.3-57.3;aliphatic index ranged of 187.5-220.0; and hydropathy (GRAVY) ranged of2.2-2.6.

These examined critical factors are within the range that we have setfor our critical factors; therefore, we are able to confirm that theaMTDs that satisfy these critical factors have relatively highcell-permeability and much higher intracellular delivery potentialcompared to reference hydrophobic CPPs reported during the past twodecades.

It has been widely evident that many human diseases are caused byproteins with deficiency or over-expression that causes mutations suchas gain-of-function or loss-of-function. If biologically active proteinscould be delivered for replacing abnormal proteins within a short timeframe, possibly within an hour or two, in a quantitative manner, thedosage may be regulated depending on when and how proteins may beneeded. By significantly improving the solubility and yield of novelaMTD in this invention (Table 31), one could expect its practicalpotential as an agent to effectively deliver therapeutic macromoleculessuch as proteins, peptides, nucleic acids, and other chemical compoundsinto live cells as well as live mammals including human. Therefore,newly developed MITT utilizing the pool (240) of novel aMTDs can be usedas a platform technology for discovery and development of protein-basedbiotherapeutics to apprehend intracellular protein therapy afterdetermining the optimal cargo-aMTD relationship.

The following examples are presented to aid practitioners of theinvention, to provide experimental support for the invention, and toprovide model protocols. In no way are these examples to be understoodto limit the invention.

Example 1. Development of Novel Advanced Macromolecule TransductionDomain (aMTD)

H-regions of signal sequences (HRSP)-derived CPPs (MTS/MTM and MTD) donot have a common sequence, a sequence motif, and/or a common structuralhomologous feature. In this invention, the aim is to develop improvedhydrophobic CPPs formatted in the common sequence and structural motifthat satisfy newly determined ‘critical factors’ to have a ‘commonfunction,’ to facilitate protein translocation across the plasmamembrane with similar mechanism to the analyzed CPPs.

The structural motif as follows:

In Table 9, universal common sequence/structural motif is provided asfollows. The amino acid length of the peptides in this invention rangesfrom 9 to 13 amino acids, mostly 12 amino acids, and their bendingpotentials are dependent with the presence and location of proline inthe middle of sequence (at 5′, 6′, 7′ or 8′ amino acid) and at the endof peptide (at 12′) for recombinant protein bending. Instability index(II) for rigidity/flexibility of aMTDs is II<40, grand average ofhydropathy (GRAVY) for hydropathy is around 2.2, and aliphatic index(AI) for structural features is around 200 (Table 9). Based on thesestandardized critical factors, new hydrophobic peptide sequences, namelyadvanced macromolecule transduction domain peptides (aMTDs), in thisinvention have been developed and summarized in Tables 10 to 15.

Example 2. Construction of Expression Vectors for Recombinant ProteinsFused to aMTDs

Our newly developed technology has enabled us to expand the method formaking cell-permeable recombinant proteins. The expression vectors weredesigned for histidine-tagged CRA proteins fused with aMTDs orrPeptides. To construct expression vectors for recombinant proteins,polymerase chain reaction (PCR) had been devised to amplify eachdesigned aMTD or rPeptide fused to CRA.

The PCR reactions (100 ng genomic DNA, 10 pmol each primer, each 0.2 mMdNTP mixture, 1× reaction buffer and 2.5 U Pfu(+) DNA polymerase (Doctorprotein, Korea) was digested on the restriction enzyme site between NdeI (5′) and Sal I (3′) involving 35 cycles of denaturation (95° C.),annealing (62° C.), and extension (72° C.) for 30 seconds each. For thelast extension cycle, the PCR reactions remained for 5 minutes at 72° C.Then, they were cloned into the site of pET-28a(+) vectors (Novagen,Darmstadt, Germany). DNA ligation was performed using T4 DNA ligase at4° C. overnight. These plasmids were mixed with competent cells of E.coli DH5-alpha strain on the ice for 10 minutes. This mixture was placedon the ice for 2 minutes after it was heat shocked in the water bath at42° C. for 90 seconds. Then, the mixture added with LB broth media wasrecovered in 37° C. shaking incubator for 1 hour. Transformant wasplated on LB broth agar plate with kanamycin (50 μg/mL) (Biopure,Johnson City, Tenn., USA) before incubating at 37° C. overnight. From asingle colony, plasmid DNA was extracted, and after the digestion of NdeI and Sal I restriction enzymes, digested DNA was confirmed at 645 bp byusing 1.2% agarose gels electrophoresis (FIG. 2). PCR primers for theCRA recombinant proteins fused to aMTD and random peptides (rPeptide)are summarized in Tables 23 to 30. Amino acid sequences of aMTD andrPeptide primers are shown in Tables 31 to 38.

Example 3. Inducible Expression, Purification and Preparation ofRecombinant Proteins Fused to aMTDs and rPeptides

To express recombinant proteins, pET-28a(+) vectors for the expressionof CRA proteins fused to a negative control [rPeptide 38 (rP38) (SEQ IDNO: 952)], reference hydrophobic CPPs (MTM₁₂ (SEQ ID NO: 953) and MTD₈₅(SEQ ID NO: 954)) and aMTDs were transformed in E. coli BL21 (DE3)strains. Cells were grown at 37° C. in LB medium containing kanamycin(50 μg/ml) with a vigorous shaking and induced at OD₆₀₀=0.6 by adding0.7 mM IPTG (Biopure) for 2 hours at 37° C. Induced recombinant proteinswere loaded on 15% SDS-PAGE gel and stained with Coomassie BrilliantBlue (InstantBlue, Expedeon, Novexin, UK) (FIG. 3).

The E. coli cultures were harvested by centrifugation at 5,000× rpm for10 minutes, and the supernatant was discarded. The pellet wasre-suspended in the lysis buffer (50 mM NaH₂PO₄, 10 mM Imidazol, 300 mMNaCl, pH 8.0). The cell lysates were sonicated on ice using a sonicator(Sonics and Materials, Inc., Newtown, Conn., USA) equipped with a probe.After centrifuging the cell lysates at 5,000× rpm for 10 minutes topellet the cellular debris, the supernatant was incubated with lysisbuffer-equilibrated Ni-NTA resin (Qiagen, Hilden, Germany) gently byopen-column system (Bio-rad, Hercules, Calif., USA). After washingprotein-bound resin with 200 ml wash buffer (50 mM NaH₂PO₄, 20 mMImidazol, 300 mM NaCl, pH 8.0), the bounded proteins were eluted withelution buffer (50 mM NaH₂PO₄, 250 mM Imidazol, 300 mM NaCl, pH 8.0).

Recombinant proteins purified under natural condition were analyzed on15% SDS-PAGE gel and stained with Coomassie Brilliant Blue (FIG. 4). Allof the recombinant proteins were dialyzed for 8 hours and overnightagainst physiological buffer, a 1:1 mixture of cell culture medium(Dulbecco's Modified Eagle's Medium: DMEM, Hyclone, Logan, Utah, USA)and Dulbecco's phosphate buffered saline (DPBS, Gibco, Grand Island,N.Y., USA). From 316 aMTDs and 141 rPeptides cloned, 240 aMTD- and 31rPeptide-fused recombinant proteins were induced, purified, prepared andanalyzed for their cell-permeability.

Example 4. Determination of Quantitative Cell-Permeability ofRecombinant Proteins

For quantitative cell-permeability, the aMTD- or rPeptide-fusedrecombinant proteins were conjugated to fluorescein isothiocyanate(FITC) according to the manufacturer's instructions (Sigma-Aldrich, St.Louis, Mo., USA). RAW 264.7 cells were treated with 10 μM FITC-labeledrecombinant proteins for 1 hour at 37° C., washed three times with coldPBS, treated with 0.25% tripsin/EDTA (Sigma-Aldrich, St. Louis, Mo.) for20 minutes at 37° C. to remove cell-surface bound proteins.Cell-permeability of these recombinant proteins were analyzed by flowcytometry (Guava, Millipore, Darmstadt, Germany) using the FlowJocytometric analysis software (FIGS. 5 to 6). The relativecell-permeability of aMTDs were measured and compared with the negativecontrol (rP38 (SEQ ID NO: 952)) and reference hydrophobic CPPs (MTM12(SEQ ID NO: 953) and MTD85 (SEQ ID NO: 954)) (Table 31).

Example 5. Determination of Cell-Permeability and IntracellularLocalization of Recombinant Proteins

For a visual reference of cell-permeability, NIH3T3 cells were culturedfor 24 hours on coverslip in 24-wells chamber slides, treated with 10 μMFITC-conjugated recombinant proteins for 1 hour at 37° C., and washedthree times with cold PBS. Treated cells were fixed in 4%paraformaldehyde (PFA, Junsei, Tokyo, Japan) for 10 minutes at roomtemperature, washed three times with PBS, and mounted with VECTASHIELDMounting Medium (Vector laboratories, Burlingame, Calif., USA), andcounter stained with DAPI (4′,6-diamidino-2-phenylindole). Theintracellular localization of the fluorescent signal was determined byconfocal laser scanning microscopy (LSM700, Zeiss, Germany; FIGS. 7 and8).

Example 6-1. Cloning of aMTD/SD-Fused SOCS3 Recombinant Protein

Full-length cDNA for human SOCS3 (SEQ ID NO: 815) was purchased fromOrigene (USA). Histidine-tagged human SOCS3 proteins were constructed byamplifying the SOCS3 cDNA (225 amino acids) using primers for aMTD fusedto SOCS3 cargo. The PCR reactions (100 ng genomic DNA, 10 pmol eachprimer, each 0.2 mM dNTP mixture, 1× reaction buffer and 2.5 U Pfu(+)DNA polymerase (Doctor protein, Korea)) were digested on the restrictionenzyme site between Nde I (5′) and Sal I (3′) involving 35 cycles ofdenaturing (95° C.), annealing (62° C.), and extending (72° C.) for 45sec each. For the last extension cycle, the PCR reactions remained for10 min at 72° C. The PCR products were subcloned into 6×His expressionvector, pET-28a(+) (Novagen, Darmstadt, Germany). Coding sequence forSDA (SEQ ID NO: 805) or SDB (SEQ ID NO: 806) fused to C terminus ofhis-tagged aMTD-SOCS3 was cloned at BamHI (5′) and Sal1 (3′) inpET-28a(+) from PCR-amplified DNA segments and confirmed by DNA sequenceanalysis of the resulting plasmids.

TABLE 36 Recombinant Cargo SD Protein 5′ Primers 3′ Primers SOCS3 — HS35′-GGAATTCCATATGGTCA 5′-CCCGGATCCTTAAAGC CCCACAGCAAGTTTCCCGCGGGGCATCGTACTGGTCC CGCC-3′ AGGAA-3′ (SEQ ID NO: 955) (SEQ ID NO: 956) —HM₁₆₅S3 5′-GGAATTCCATATGGCGCT 5′-CCCGGATCCTTAAAG GGCGGTGCCGGTGGCGCTCGGGGCATCGTACTGGT GGCGATTGTGCCGGTCACC CCAGGAA-3′ CACAGCAAGTTTC-3′(SEQ ID NO: 958) (SEQ ID NO: 957) A HM₁₆₅S3A 5′-GGAATTCCATATGGCGC5′-CGCGTCGACTTACCTCG TGGCGGTGCCGGTGGCGC GCTGCACCGG CACGGAGTGGCGATTGTGCCGGTCAC ATGAC-3′ CCACAGCAAGTTTC-3′ (SEQ ID NO: 960)(SEQ ID NO: 959) B HM₁₆₅S3B 5′-GGAATTCCATATGGCGCT 5′-CGCGTCGACTTAAAGGGGCGGTGCCGGTGGCGCT GTTTCCGAAGGCTTGGCT GGCGATTGTGCCGGTCACC ATCTT-3′CACAGCAAGTTTC-3′ (SEQ ID NO: 962) (SEQ ID NO: 961) C HM₁₆₅S3C5′-GGAATTCCATATGGCGC 5′-GCGTCGACTTAGGC TGGCGGTGCCGGTGGCGCTCAGGTTAGCGTCGAG-3′ GGCGATTGTGCCGGTCACC (SEQ ID NO: 964) CACAGCAAGTTTC-3′(SEQ ID NO: 963) D HM₁₆₅S3D 5′-GGAATTCCATATGGCGC 5′-GCGTCGACTTATTTTTGGCGGTGCCGGTGGCGC TTCTCGGACAGATA-3′ TGGCGATTGTGCCGGTCAC(SEQ ID NO: 966) CCACAGCAAGTTTC-3′ (SEQ ID NO: 965) E  HM₁₆₅S3E5′-GGAATTCCATATGGCGC 5′-ACGCGTCGACTTAA TGGCGGTGCCGGTGGCGCCCTCCAATCTGTTCGCG TGGCGATTGTGCCGGTCA GTGAGCCTC-3′ CCCACAGCAAGTTTC-3′(SEQ ID NO: 968) (SEQ ID NO: 967)

Example 6-2. Preparation of aMTD/SD-Fused SOCS3 Recombinant Protein

To determine a stable structure of the cell-permeable aMTD/SD-fusedSOCS3 recombinant protein, a pET-28a(+) vector and an E. coliBL21-CodonPlus (DE3)-RIL were subjected to the following experiment.

Each of the recombinant expression vectors, HS3, HMS3, HMS3A, HMS3B,HMS3C, HMS3D, and HMS3E prepared in example 6-1 was transformed into E.coli BL21 CodonPlus(DE3)-RIL by a heat shock method, and then 600 ul ofeach was incubated in an LB medium (Biopure, Johnson City, Tenn., USA)containing 50 μg/ml of kanamycin at 37° C. for 1 hour. Thereafter, therecombinant protein gene-introduced E. coli was inoculated in 7 ml of LBmedium, and then incubated at 37° C. overnight. The E. coli wasinoculated in 700 ml of LB medium and incubated at 37° C. until OD₆₀₀reached 0.6. To this culture medium, 0.6 mM ofisopropyl-β-D-thiogalactoside (IPTG, Gen Depot, USA) was added as aprotein expression inducer, followed by further incubation at 37° C. for3 hours. This culture medium was centrifuged at 4° C. and 8,000 rpm for10 minutes and a supernatant was discarded to recover a cell pellet. Thecell pellet thus recovered was suspended in a lysis buffer (100 mMNaH₂PO₄, 10 mM Tris-HCl, 8 M Urea, pH 8.0), and cells were disrupted bysonication (on/off time: 30 sec/30 sec, on time 2 hours, amplify 40%),and centrifuged at 15,000 rpm for 30 min to obtain a soluble fractionand an insoluble fraction.

This insoluble fraction was suspended in a denature lysis buffer (8 MUrea, 10 mM Tris, 100 mM Sodium phosphate) and purified by Ni²⁺ affinitychromatography as directed by the supplier(Qiagen, Hilden, Germany) andrefolded by dialyzing with a refolding buffer (0.55 M guanidine HCl,0.44 M L-arginine, 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 100 mM NDSB,2 mM reduced glutathione, and 0.2 mM oxidized glutathione). Afterpurification, the proteins were put in a SnakeSkin Dialysis Tubing bag(pore size: 10000 mw, Thermo scientific, USA) and then they weredialyzed by physiological buffer (DMEM). The strain lysate where proteinexpression was not induced, the strain lysate where protein expressionwas induced by addition of IPTG, and purified proteins were loaded onSDS-PAGE to analyze protein expression characteristics and expressionlevels (FIGS. 19 and 20).

As shown in FIG. 19, it was confirmed that SOCS3 recombinant proteinsshowed high expression levels in the BL21CodonPlus(DE3)-RIL strain.SOCS3 recombinant proteins containing aMTD₁₆₅ (SEQ ID NO: 43) andsolubilization domain (SDA of SEQ ID NO: 798 or SDB of SEQ ID NO: 799)(HM₁₆₅S3A and HM₁₆₅S3B (SEQ ID NO: 827)) had little tendency toprecipitate whereas recombinant SOCS3 proteins lacking a solubilizationdomain (HM₁₆₅S3) or lacking an aMTD and a SD (HS3) were largelyinsoluble. Solubility of aMTD/SD-fused SOCS3 proteins was scored on a 5point scale compared with that of SOCS3 proteins lacking thesolubilization domain.

Example 6-3. Determination of Solubility/Yield of aMTD/SD-Fused SOCS3Recombinant Proteins According to SD Type

To determine aMTD/SD-fused SOCS3 recombinant proteins having optimalcell-permeability, solubilization domains were replaced in the samemanner as in Example 6-2 to prepare 5 kinds of aMTD/SD-fused SOCS3recombinant proteins, and their solubility/yield were measured (FIG.19).

As shown in FIG. 19, it was confirmed that the aMTD/SD-fused SOCS3recombinant protein prepared by fusing with SDB (SEQ ID NO: 799) amongthe different SDs showed the highest solubility/yield. Therefore, theSDB (SEQ ID NO: 799)-fused iCP-SOCS3 recombinant protein was used in thesubsequent experiment.

Example 6-4. Comparison Between aMTD/SD-Fused SOCS3 Recombinant Proteinand Basic CPP/SD-Fused SOCS3 Recombinant Protein

To compare the solubility/yield, cell/tissue-permeability, mechanism ofcytopermeability of aMTD/SD-fused SOCS3 recombinant proteins to those ofconventional basic CPP/SD-fused SOCS3 recombinant proteins, cloning,preparation, and measurement of solubility/yield of the basicCPP/SD-fused SOCS3 recombinant proteins were performed in the samemanner as in Examples 6-1 to 6-3 except for a known basic CPP (TAT orPolyR) being used instead of aMTD. Sequences of amino acids andnucleotides of basic CPP, and the primers used in this example are shownin FIG. 71.

The solubility/yield of aMTD165 (SEQ ID NO: 43)/SD-fused SOCS3recombinant proteins was much higher than that of TAT/SD-fused SOCS3 orPolyR/SD-fused SOCS3 recombinant proteins (FIG. 72).

Example 7-1. Cell-Permeability

To examine cell-permeability of SOCS3 recombinant protein, SOCS3recombinant proteins were conjugated to 5/6-fluorescein isothiocyanate(FITC). RAW 264.7 (KCLB, Seoul, South Korea) (FIG. 20) or NIH3T3 cells(KCLB, Seoul, South Korea) (FIG. 21) were treated with 10 μMFITC-labeled SOCS3 recombinant proteins and cultivated for 1 hr at 37°C. In this regard, RAW 264.7 cells were cultured in a DMEM mediumcontaining 10% fetal bovine serum (FBS, Hyclone, USA) and 1%penicillin/streptomycin (Hyclone, USA).

After cultivation, the cells were washed three times with ice-cold PBS(Phosphate-buffered saline, Hyclone, USA) and treated with proteinase K(10 μg/mL, SIGMA, USA) to remove surface-bound proteins, andinternalized proteins were measured by flow cytometry (FlowJo cytometricanalysis software, Guava, Millipore, Darmstadt, Germany). Untreatedcells (gray) and equimolar concentration of unconjugated FITC (FITConly, green)-treated cells were served as control (FIG. 20). Each ofNIH3T3 cells was incubated for 1 hour at 37° C. with 10 μM FITC-labeledSOCS3 protein. For nuclear staining, a mixture of VECTASHIELD MountingMedium (Vector laboratories, Burlingame, Calif.) and DAPI(4′,6-diamidino-2-phenylindole) was added to NIH3T3 cells, andvisualized using a confocal laser microscope (LSM700, Zeiss, Germany)(FIG. 21).

As shown in FIGS. 20 and 21, SOCS3 recombinant proteins containingaMTD₁₆₅ (SEQ ID NO: 43) (HM₁₆₅S3, HM₁₆₅S3A and HM₁₆₅S3B (SEQ ID NO:827)) efficiently entered the cells (FIGS. 20 and 21) and were localizedto various extents in cytoplasm (FIG. 21). In contrast, SOCS3 proteincontaining non-aMTD (HS3) did not appear to enter cells. While all SOCS3proteins containing aMTD₁₆₅ (SEQ ID NO: 43) transduced into the cells,HM₁₆₅S3B (SEQ ID NO: 827) displayed more uniform cellular distribution,and protein uptake of HM₁₆₅S3B (SEQ ID NO: 827) was also very efficient.

Example 7-1-2. Comparison Between aMTD/SD-Fused SOCS3 RecombinantProtein and Basic CPP/SD-Fused SOCS3 Recombinant Protein

The cell-permeability of basic CPP/SD-fused SOCS3 recombinant proteinswas assessed by the same method as used in Example 7-1 except for aknown basic CPP (TAT or PolyR) being used instead of aMTD. The resultsof the assessment were shown in FIG. 73.

According to the results, all recombinant proteins exhibitedcell-permeability. Among the proteins, aMTD165 (SEQ ID NO: 43)/SDB (SEQID NO: 799)-fused SOCS3 recombinant protein (HM₁₆₅S3B) (SEQ ID NO: 827)showed the highest cell-permeability.

Example 7-2. Tissue-Permeability

To further investigate in vivo delivery of SOCS3 recombinant proteins,ICR mice (Doo-Yeol Biotech Co. Ltd., Seoul, Korea) were intraperitoneal(IP) injected with 600 μg/head of 10 μM FITC (Fluoresceinisothiocyanate, SIGMA, USA)-labeled SOCS3 proteins and sacrificed after2 hrs. From the mice, the liver, kidney, spleen, lung, heart, and brainwere removed and washed with PBS, and then placed on a dry ice, andembedded with an O.C.T. compound (Sakura). After cryosectioning at 20μm, tissue distributions of fluorescence-labeled-SOCS3 proteins indifferent organs was analyzed by fluorescence microscopy (Carl Zeiss,Gottingen, Germany)(FIG. 22).

As shown in FIG. 22, SOCS3 recombinant proteins fused to aMTD₁₆₅ (SEQ IDNO: 43) (HM₁₆₅S3, HM₁₆₅S3A and HM₁₆₅S3B (SEQ ID NO: 827)) weredistributed to a variety of tissues (liver, kidney, spleen, lung, heartand, to a lesser extent, brain). Liver showed highest levels offluorescent cell-permeable SOCS3 since intraperitoneal administrationfavors the delivery of proteins to this organ via the portalcirculation. SOCS3 containing aMTD₁₆₅ (SEQ ID NO: 43) was detectable toa lesser degree in lung, spleen and heart. aMTD/SDB (SEQ ID NO:799)-fused SOCS3 recombinant protein (HM₁₆₅S3B) (SEQ ID NO: 827) showedthe highest systemic delivery of SOCS3 protein to the tissues comparedto the SOCS3 containing only aMTD of SEQ ID NO: 43 (HM₁₆₅S3) or aMTD(SEQ ID NO: 43)/SDA (SEQ ID NO: 798) (HM₁₆₅S3A) proteins. These datasuggest that SOCS3 protein containing both of aMTD₁₆₅ (SEQ ID NO: 43)and SDB (SEQ ID NO: 799) leads to higher cell-/tissue-permeability dueto the increase in solubility and stability of the protein, and itdisplayed a dramatic synergic effect on cell-/tissue-permeability.

Example 7-2-2. Comparison Between aMTD/SD-Fused SOCS3 RecombinantProtein and Basic CPP/SD-Fused SOCS3 Recombinant Protein

The tissue-permeability of basic CPP/SD-fused SOCS3 recombinant proteinswas assessed by the same method as used in Example 7-2 except for aknown basic CPP (TAT or PolyR) being used instead of aMTD. The resultsof the assessment were shown in FIG. 74.

According to the results, only aMTD165 (SEQ ID NO: 43)/SDB (SEQ ID NO:799)-fused SOCS3 recombinant protein (HM₁₆₅S3B) (SEQ ID NO: 827)exhibited superior cell-permeability.

Example 8-1 Biological Activity Test of iCP-SOCS3-Inhibition Activity ofIFN-γ-Induced STAT Phosphorylation

It was examined whether the iCP-SOCS3 recombinant proteins prepared byfusion with combinations of aMTD and SD inhibits activation of theJAK/STAT-signaling pathway.

PANC-1 Cells (KCLB, Seoul, South Korea) were treated with 10 ng/ml IFN-γ(R&D systems, Abingdon, UK) for 15 min and treated with either DMEM(vehicle) or 10 μM aMTD/SD-fused SOCS3 recombinant proteins for 2 hrs.The cells were lysed in RIPA lysis buffer (Biosesang, Seongnam, Korea)containing proteinase inhibitor cocktail (Roche, Indianapolis, Ind.,USA), incubated for 15 min at 4° C., and centrifuged at 13,000 rpm for10 min at 4° C. Equal amounts of lysates were separated on 10% SDS-PAGEgels and transferred to a nitrocellulose membrane. The membranes wereblocked using 5% skim milk in TBST and for western blot analysisincubated with the following antibodies: anti-phospho-STAT1 (CellSignaling Technology, USA) and anti-phospho-STAT3 (Cell SignalingTechnology, USA), then HRP conjugated anti-rabbit secondary antibody(Santacruz).

The well-known cytokine signaling inhibitory actions of SOCS3 areinflammation inhibition through i) inhibition of IFN-γ-mediated JAK/STATand ii) inhibition of LPS-mediated cytokine secretion. The ultimate testof cell-penetrating efficiency is a determination of intracellularactivity of SOCS3 proteins transported by aMTD. Since endogenous SOCS3are known to block phosphorylation of STAT1 and STAT3 by IFN-γ-mediatedJanus kinases (JAK) 1 and 2 activation, we demonstrated whethercell-permeable SOCS3 inhibits the phosphorylation of STATs. As shown inFIG. 23, All SOCS3 recombinant proteins containing aMTD165 of SEQ ID NO:43 (HM₁₆₅S3, HM₁₆₅S3A and HM₁₆₅S3B (SEQ ID NO: 827)), suppressedIFN-γ-induced phosphorylation of STAT1 and STAT3. In contrast, STATphosphorylation was readily detected in cells exposed to HS3, whichlacks the aMTD motif required for membrane penetration, indicating thatHS3, which lacks an MTD sequence did not enter the cells, has nobiological activity.

Example 8-2. Biological Activity Test of iCP-SOCS3

Peritoneal macrophages were obtained from C3H/HeJ mice (Doo-Yeol BiotechCo. Ltd. Korea) Peritoneal macrophages were incubated with 10 μM SOCS3recombinant proteins (1:HS3, 2:HM₁₆₅S3, 3:HM₁₆₅S3A and 4:HM₁₆₅S3B (SEQID NO: 827), respectively)(here, aMTD165 has an amino acid sequence ofSEQ ID NO: 43, SDA has an amino acid sequence of SEQ ID NO:798, and SDBhas an amino acid sequence of SEQ ID NO: 799) for 1 hr at 37° C. andthen stimulated them with LPS (Lipopolysaccharide)(500 ng/ml) and/orIFN-γ (100 U/ml) without removing iCP-SOCS3 proteins for 3, 6, or 9 hrs.The culture media were collected, and the extracellular levels ofcytokine (TNF-α, IL-6) were measured by a cytometric bead array (BDPharmingen, San Diego, Calif., USA) according to the manufacturer'sinstructions.

The effect of cell-permeable SOCS3 proteins on cytokines secretion wasinvestigated. Treatment of C3H/HeJ primary peritoneal macrophages withSOCS3 proteins containing aMTD₁₆₅ (SEQ ID NO: 43) suppressed TNF-α andIL-6 secretion induced by the combination of IFN-γ and LPS by 50-90%during subsequent 9 hrs of incubation (FIG. 24). In particular, aMTD₁₆₅(SEQ ID NO: 43)/SDB (SEQ ID NO: 799)-fused SOCS3 recombinant proteinshowed the greatest inhibitory effect on cytokine secretion. Incontrast, cytokine secretion in macrophages treated with non-permeableSOCS3 protein (HS3) was unchanged, indicating that recombinant SOCS3lacking the aMTD doesn't affect intracellular signaling. Therefore, weconclude that differences in the biological activities of HM₁₆₅S3B (SEQID NO: 827) as compared to HS3B are due to the differences in proteinuptake mediated by the aMTD sequence. In light of solubility/yield,cell-/tissue-permeability, and biological effect, SOCS3 recombinantprotein containing aMTD165 (SEQ ID NO: 43) and SDB (SEQ ID NO: 799)(HM₁₆₅S3B) (SEQ ID NO: 827) is a prototype of a new generation ofimproved cell-permeable SOCS3 (iCP-SOCS3), and will be selected forfurther evaluation as a potential anti-tumor agent.

Example 9. Preparation of Control Protein (Non-CP-SOCS3: HS3BRecombinant Protein)

As an experimental negative control, a SOCS3 recombinant protein havingno cell permeability was prepared.

According to example 6-2, SOCS3 recombinant proteins lacking SD(HM₁₆₅S3) or both aMTD and SD (HS3) were found to be less soluble,produced lower yields, and showed tendency to precipitate when they wereexpressed and purified in E. coli. Therefore, we additionally designedand constructed SOCS3 recombinant protein containing only SDB (SEQ IDNO: 799) (without aMTD₁₆₅ (SEQ ID NO: 43): HS3B) as a negative control(FIG. 25). Preparation, expression and purification, and measurement ofsolubility/yield of the recombinant proteins were performed in the samemanner as in Examples 6-2 and 6-3.

TABLE 37 Recombinant Cargo SD Protein 5′ Primers 3′ Primers SOCS3 B HS3B5′-GGAATTCCATATGGTC 5′-CGCGTCGACTTAAA ACCCACAGCAAGTTTCC GGGTTTCCGAAGGCTTCGCCGCC-3′ GGCTATCTT-3′ (SEQ ID NO: 969) (SEQ ID NO: 970)

As expected, its solubility and yield increased compared to that ofSOCS3 proteins lacking SDB (SEQ ID NO: 799) (HS3; FIG. 26). Therefore,HS3B proteins were used as a control protein.

Example 10. Selection of aMTD for Cell-Permeability

After a basic structure of the stable recombinant proteins fused withcombinations of aMTD and SD was determined, 22 aMTDs were selected fordevelopment of iCP-SOCS3 recombinant protein (Tables 38 and 39), basedon the critical Factors, in order to examine which aMTD provides thehighest cell-/tissue-permeability.

For comparison, 5 kinds of random peptides that do not satisfying one ormore critical factors were selected (Table 40).

Solubility/yield and cell-permeability of 22 kinds of aMTD/SDB (SEQ IDNO: 799)-fused SOCS3 recombinant proteins, prepared by using primers ofTable 41 in the same manner as in Example 6-2, were analyzed accordingto Examples 6-3 and 7-1, respectively.

TABLE 38 aMTD Amino Acid Cargo ID Sequence 5' Primers 3' Primers SOCS3MTM AAVLIPALAAP GGAATTCCATATGGCGGCGGTGCTGCTGCCG CGCGTCGACTT(SEQ ID NO: 865) GTGCTGCTGGCGGCGCCGGTCACCCACAGCA TAAAGGGTTTCAGTTTCCCGCCGCC (SEQ ID NO: 979) CGAAGGCTTGG CTATCT (SEQ ID NO: 1003) 44ALAVPVALLVAP GGAATTCCATATGGCGCTGGCGGTGCCGGTGG (SEQ ID NO: 16)CGCTGCTGGTGGCGCCGGTCACCCACAGCAAG TTTCCCGCCGCC (SEQ ID NO: 980) 81AALLPALAALLP GGAATTCCATATGGCGGCGCTGCTGCCGGCGC (SEQ ID NO: 22)TGGCGGCGCTGCTGCCGGTCACCCACAGCAAG TTTCCCGCCGCC (SEQ ID NO: 981) 123AAIIVPAALLAP GGAATTCCATATGGCGGCGATTATTGTGCCGGC (SEQ ID NO: 33)GGCGCTGCTGGCGCCGGTCACCCACAGCAAGT TTCCCGCCGCC (SEQ ID NO: 982) 162AVVALPAALIVP GGAATTCCATATGGCGGTGGTGGCGCTGCCGG (SEQ ID NO: 40)CGGCGCTGATTGTGCCGGTCACCCACAGCAAG TTTCCCGCCGCC (SEQ ID NO: 983) 281ALIVLPAAVAVP GGAATTCCATATGGCGCTGATTGTGCTGCCGGC (SEQ ID NO: 65)GGCGGTGGCGGTGCCGGTCACCCACAGCAAGT TTCCCGCCGCC (SEQ ID NO: 984) 324 IVAVALPAALVP GGAATTCCATATGATTGTGGCGGTGGCGCTGCC (SEQ ID NO: 77)GGCGGCGCTGGTGCCGGTCACCCACAGCAAGT TTCCCGCCGCC (SEQ ID NO: 985) 364LVAAVAPALIVP GGAATTCCATATGCTGGTGGCGGCGGTGGCGC (SEQ ID NO: 85)CGGCGCTGATTGTGCCGGTCACCCACAGCAAG TTTCCCGCCGCC (SEQ ID NO: 986) 365AVIVVAPALLAP GGAATTCCATATGGCGGTGATTGTGGTGGCGCC (SEQ ID NO: 86)GGCGCTGCTGGCGCCGGTCACCCACAGCAAGT TTCCCGCCGCC (SEQ ID NO: 987) 622ALIVLAAPVAVP GGAATTCCATATGGCGCTGATTGTGCTGGCGGC (SEQ ID NO: 142)GCCGGTGGCGGTGCCGGTCACCCACAGCAAGT TTCCCGCCGCC (SEQ ID NO: 988) 662ALAVILAPVAVP GGAATTCCATATGGCGCTGGCGGTGATTCTGGC (SEQ ID NO: 822)GCCGGTGGCGGTGCCGGTCACCCACAGCAAGT TTCCCGCCGCC (SEQ ID NO: 989) 563ALAVIVVPALAP GGAATTCCATATGGCGCTGGCGGTGATTGTGGT (SEQ ID NO: 131)GCCGGCGCTGGCGCCGGTCACCCACAGCAAGT TTCCCGCCGCC (SEQ ID NO: 990) 899AVVIALPAVVAP GGAATTCCATATGGCGGTGGTGATTGCGCTGCC (SEQ ID NO: 229)GGCGGTGGTGGCGCCGGTCACCCACAGCAAGT TTCCCGCCGCC (SEQ ID NO: 991) 897AVIVPVAIIAAP GGAATTCCATATGGCGGTGATTGTGCCGGTGGC (SEQ ID NO: 228)GATTATTGCGGCGCCGGTCACCCACAGCAAGT TTCCCGCCGCC (SEQ ID NO: 992) 623VAAAIALPAIVP GGAATTCCATATGGTGGCGGCGGCGATTGCGC (SEQ ID NO: 143)TGCCGGCGATTGTGCCGGTCACCCACAGCAAG TTTCCCGCCGCC (SEQ ID NO: 993) 908VALALAPVVVAP GGAATTCCATATGGTGGCGCTGGCGCTGGCGC (SEQ ID NO: 237)CGGTGGTGGTGGCGCCGGTCACCCACAGCAAG TTTCCCGCCGCC (SEQ ID NO: 994) 911VALALPAVVVAP GGAATTCCATATGGTGGCGCTGGCGCTGCCGG (SEQ ID NO: 239)CGGTGGTGGTGGCGCCGGTCACCCACAGCAAG TTTCCCGCCGCC (SEQ ID NO: 995) 2AAAVPLLAVVVP GGAATTCCATATGGCGGCGGCGGTGCCGCTGC (SEQ ID NO: 2)TGGCGGTGGTGGTGCCGGTCACCCACAGCAAG TTTCCCGCCGCC (SEQ ID NO: 996) 904AVLAVVAPVVAP GGAATTCCATATGGCGGTGCTGGCGGTGGTGG (SEQ ID NO: 233)CGCCGGTGGTGGCGCCGGTCACCCACAGCAAG TTTCCCGCCGCC (SEQ ID NO: 997) 481AIAIAIVPVALP GGAATTCCATATGGCGATTGCGATTGCGATTGT (SEQ ID NOl 110)GCCGGTGGCGCTGCCGGTCACCCACAGCAAGT TTCCCGCCGCC (SEQ ID NO: 998) 787AVALVPVIVAAP GGAATTCCATATGGCGGTGGCGCTGGTGCCGG (SEQ ID NO: 177)TGATTGTGGCGGCGCCGGTCACCCACAGCAAG TTTCCCGCCGCC (SEQ ID NO: 999) 264LAAAPVVIVIAP GGAATTCCATATGCTGGCGGCGGCGCCGGTGG (SEQ ID NO: 63)TGATTGTGATTGCGCCGGTCACCCACAGCAAGT TTCCCGCCGCC (SEQ ID NO: 1000) 363LAAAPVVIVIAP GGAATTCCATATGGCGGTGCTGGCGGTGGCGC (SEQ ID NO: 63)CGGCGCTGATTGTGCCGGTCACCCACAGCAAG TTTCCCGCCGCC (SEQ ID NO: 1001) 121AIVALPALALAP GGAATTCCATATGGCGATTGTGGCGCTGCCGGC (SEQ ID NO: 32)GCTGGCGCTGGCGCCGGTCACCCACAGCAAGT TTCCCGCCGCC (SEQ ID NO: 1002)

rPeptide Amino Acid Cargo ID Sequence 5' Primers 3' Primers SOCS3 921IWWFVVLPLVVP GGAATTCCATATGATTTGGTGGTTTGTGGTGC CGCGTCGACT (SEQ ID NO: TGCCGCTGGTGGTGCCGGTCACCCACAGCAA TAAAGGGTTT 931)GTTTCCCGCCGCC (SEQ ID NO: 1004) CCGAAGGCTT GGCTATCTT (SEQ ID NO:  1009)16 NNSCTTYTNGSQ GGAATTCCATATGAACAACAGCTGCACCACC (SEQ ID NO: TATACCAACGGCAGCCAGGTCACCCACAGCA 890) AGTTTCCCGCCGCC (SEQ ID NO: 1005) 67LDAEVPLADDVP GGAATTCCATATGCTGGATGCGGAAGTGCCG (SEQ ID NO: CTGGCGGATGATGTGCCGGTCACCCACAGCA 906) AGTTTCCCGCCGCC (SEQ ID NO: 1006) 29VLPPLPVLPVLP GGAATTCCATATGGTGCTGCCGCCGCTGCCGG (SEQ ID NO: TGCTGCCGGTGCTGCCGGTCACCCACAGCAA 924) GTTTCCCGCCGCC (SEQ ID NO: 1007) 700GTSNTSCQSNQNS GGAATTCCATATGGGCACCAGCAACACCTGC (SEQ ID NO: CAGAGCAACCAGAACAGCGTCACCCACAGC 951) AAGTTTCCCGCCGCC (SEQ ID NO: 1008)

As shown in FIGS. 27 to 34, it was confirmed that most of the aMTD/SDB(SEQ ID NO: 799)-fused SOCS3 recombinant proteins showed high solubilityand yield and high cell permeability by aMTD. However, Randompeptide-SOCS3-SDB (SEQ ID NO: 799) recombinant protein showed remarkablylow cell permeability.

Example 11-1. Investigation of Biological Activity for Determination ofOptimal aMTD-Fused SOCS3 Recombinant Protein-1

Four kinds of aMTD/SD-fused SOCS3 recombinant proteins having high cellpermeability and one kind of aMTD/SD-fused SOCS3 recombinant proteinhaving the lowest cell permeability were selected, and their biologicalactivity was analyzed.

PANC-1 cells (pancreatic carcinoma cell line) were seeded in a 16-wellchamber slide at a density of 5×10³ cells/well, and then treated with 10uM of aMTD/SD-fused SOCS3 for 24 hours. Apoptotic cells were analyzedusing terminal dUTP nick-end labeling (TUNEL) assay with In Situ CellDeath Detection kit TMR red (Roche, 4056 Basel, Switzerland). Cells weretreated with either 10 μM SOCS3 recombinant protein or buffer alone for16 hrs with 2% fetal bovine serum (Hyclone, Logan, Utah, USA). Treatedcells were washed with cold PBS two times, fixed in 4% paraformaldehyde(PFA, Junsei, Tokyo, Japan) for 1 hr at room temperature, and incubatedin 0.1% Triton X-100 for 2 min on the ice. Cells were washed with coldPBS twice, and treated TUNEL reaction mixture for 1 hr at 37° C. indark, washed cold PBS three times and observed by fluorescencemicroscopy (Nikon, Tokyo, Japan).

As shown in FIG. 35, most of the aMTD/SDB (SEQ ID NO: 799)-fused SOCS3recombinant proteins induced cell death of pancreatic carcinoma cells,and of them, aMTD₁₆₅ (SEQ ID NO: 43) or aMTD₃₂₄ (SEQ ID NO: 77)-fusedSOCS3 recombinant protein induced death of the largest number of cancercells.

Example 11-2. Investigation of Biological Activity for Determination ofOptimal aMTD-Fused SOCS3 Recombinant Protein-2

AGS cells (gastric carcinoma cell line) (American Type CultureCollection; ATCC) were seeded in a 12-well plate at a density of 1×10⁵cells/well, and then treated with 10 uM of aMTD/SD-fused SOCS3 for 14hours. Cancer cell death was analyzed by Annexin V analysis. AnnexinV/7-Aminoactinomycin D (7-AAD) staining was performed using flowcytometry according to the manufacturer's guidelines (BD Pharmingen, SanDiego, Calif., USA). Briefly, cells were washed three times withice-cold PBS. The cells were then resuspended in 100 μl of bindingbuffer and incubated with 1 μl of 7-AAD and 1 μl of annexin V-PE for 30min in the dark at 37° C. Flow cytometric analysis was immediatelyperformed using a guava easyCyte™ 8 Instrument (Merck Millipore,Darmstadt, Germany).

As shown in FIG. 36, most of the aMTD/SDB (SEQ ID NO: 799)-fused SOCS3recombinant proteins induced cell death of gastric carcinoma cells, andof them, aMTD₁₆₅ (SEQ ID NO: 43) or aMTD₂₈₁(SEQ ID NO: 65)-fused SOCS3recombinant protein induced death of the largest number of cancer cells.

Example 11-3. Investigation of Biological Activity for Determination ofOptimal aMTD-Fused SOCS3 Recombinant Protein-3

AGS cells (gastric cancer cell line) were seeded in a 12 well plate at adensity of 2.5×10⁵ per well, grown to 90% confluence. Confluent AGScells were incubated with 10 μM HM # S3B in serum-free medium for 2 hrsprior to changing the growth medium (DMEM/F12, Hyclone, Logan, Utah,USA) and washed twice with PBS, and the monolayer at the center of thewell was “wounded” by scraping with a sterilized white pipette tip.Cells were cultured for an additional 24 hrs and cell migration wasobserved by phase contrast microscopy (Nikon, ECLIPSE Ts2). Themigration was quantified by counting the number of cells that migratedfrom the wound edge into the clear area.

As shown in FIG. 37, most of the aMTD/SDB (SEQ ID NO: 799)-fused SOCS3recombinant proteins inhibited cell migration of gastric carcinomacells, and of them, aMTD₁₆₅ (SEQ ID NO: 43) or aMTD₉₀₄ (SEQ ID NO:233)-fused SOCS3 recombinant protein showed the most effectiveinhibition of cancer cell migration.

Solubility/yield, permeability, and biological activity of 22 kinds ofthe aMTD-fused recombinant proteins were examined in Examples 10 to11-3, and as a result, the aMTD₁₆₅ (SEQ ID NO: 43)/SDB (SEQ ID NO:799)-fused SOCS3 recombinant protein was found to show the mostexcellent effect (FIG. 38). Therefore, the aMTD₁₆₅ (SEQ ID NO: 43)-fusedrecombinant protein was used in the subsequent experiment.

Example 12-1. Preparation of iCP-SOCS3 Recombinant Protein andInvestigation of Equivalence Thereof-1

To develop a new drug as an anticancer agent, His-tag-removed iCP-SOCS3recombinant protein was prepared and equivalence of His-Tag+, −iCP-SOCS3was investigated.

Histidine-tag free human SOCS3 proteins were constructed by amplifyingthe SOCS3 cDNA (225 amino acids) for aMTD fused to SOCS3 cargo. The PCRreactions (100 ng genomic DNA, 10 pmol each primer, each 0.2 mM dNTPmixture, 1× reaction buffer and 2.5 U Pfu(+) DNA polymerase (Doctorprotein, Korea)) were digested on the restriction enzyme site betweenNde I (5′) and Sal I (3′) involving 35 cycles of denaturing (95° C.),annealing (62° C.), and extending (72° C.) for 45 sec each. For the lastextension cycle, the PCR reactions remained for 10 min at 72° C. The PCRproducts were subcloned into pET-26b(+) (Novagen, Darmstadt, Germany).Coding sequence for SDB (SEQ ID NO: 806) fused to C terminus ofaMTD-SOCS3 was cloned at BamHI (5′) and Sal1 (3′) in pET-26b(+) fromPCR-amplified DNA segments and confirmed by DNA sequence analysis of theresulting plasmids.

TABLE 39 Recombinant Cargo SD Protein 5′ Primers 3′ primers SOCS3 — HS35′-GGAATTCCATATGGTCACCCAC 5′-CCCGGATCCTTAAA AGCAAGTTTCCCGCCGCC-3′GCGGGGCATCGTAC (SEQ ID NO: 955) TGGTCCAGGAA-3′ (SEQ ID NO: 956) —HM₁₆₅S3 5′-GGAATTCCATATGGCGCTGGCG 5′-CCCGGATCCTTAAAGTGCCGGTGGCGCTGGCGATT GCGGGGCATCGTAC GTGCCGGTCACCCACAGCAAGTGGTCCAGGAA-3′ TTTC-3′ (SEQ ID NO: 958) (SEQ ID NO: 957) A HM₁₆₅S3A5′-GGAATTCCATATGGCGCTGGCG 5′-CGCGTCGACTTACC GTGCCGGTGGCGCTGGCGATTTCGGCTGCACCGCC GTGCCGGTCACCCACAGCAAG ACGGCGATAC-3′TTTC-3′ (SEQ ID NO: 959) (SEQ ID NO: 960) B HM₁₆₅S3B5′-GGAATTCCATATGGCGCTGGCG 5′-CGCGTCGACTTAAA GTGCCGGTGGCGCTGGCGATTGGGTTTCCGAAGGC GTGCCGGTCACCCACAGCAAG TTGGCTATCTT-3′TTTC-3′ (SEQ ID NO: 961) (SEQ ID NO: 962) C HM₁₆₅S3C5′-GGAATTCCATATGGCGCTGGCG 5′-GCGTCGACTTAGGC GTGCCGGTGGCGCTGGCGATTCAGGTTAGCGTCGAG-3′ GTGCCGGTCACCCACAGCAAG (SEQ ID NO: 964)TTTC-3′ (SEQ ID NO: 965) D HM₁₆₅S3D 5′-GGAATTCCATATGGCGCTGGCG5′-GCGTCGACTTATTTT GTGCCGGTGGCGCTGGCGATT TTCTCGGACAGATA3′GTGCCGGTCACCCACAGCAAG (SEQ ID NO: 966) TTTC-3′ (SEQ ID NO: 965) EHM₁₆₅S3E 5′-GGAATTCCATATGGCGCTGGCG 5′-ACGCGTCGACTTAAGTGCCGGTGGCGCTGGCGATT CCTCCAATCTGTTC GTGCCGGTCACCCACAGCAAGGCGGTGAGCCTC-3′ TTTC-3′ (SEQ ID NO: 967) (SEQ ID NO: 968) B M₁₆₅S3B5′-GGAATTCCATATGGCGCTGGCG 5′-CGCGTCGACTTAAA GTGCCGGTGGCGCTGGCGATTGGGTTTCCGAAGGC GTGCCGGTCACCCACAGCAAG TTGGCTATCTT-3′  TTTC-3′ (SEQ ID NO: 977) (SEQ ID NO: 978)

Expression, purification and solubility/yield were measured in the samemanner as in Example 6-2 to 6-3, and as a result, his-tag-removedM₁₆₅S3B was found to have high solubility/yield (FIG. 39).

Example 12-2. Preparation of iCP-SOCS3 Recombinant Protein andInvestigation of Equivalence Thereof-2

In the same manner as in Example 7-1, RAW264.7 cells were treated withFITC-labeled HS3B, HM₁₆₅S3B, and M₁₆₅S3B proteins, and cell permeabilitywas evaluated.

As shown in FIG. 40, both HM₁₆₅S3B (SEQ ID NO: 827) and M₁₆₅S3B werefound to have high cell permeability.

Example 12-3. Preparation of iCP-SOCS3 Recombinant Protein andInvestigation of Equivalence Thereof-3

To investigate biological activity equivalence of the HM₁₆₅S3B (SEQ IDNO: 827) and M₁₆₅S3B recombinant proteins, induction of apoptosis ofgastric carcinoma cell line (AGS) was analyzed by Annexin V staining inthe same manner as in Example 11-2, and inhibition of migration wasanalyzed in the same manner as in Example 11-3.

As shown in FIG. 41, it was confirmed that both HM₁₆₅S3B (SEQ ID NO:827) and M₁₆₅S3B showed high anticancer efficacy and M₁₆₅S3B exhibitedefficacy equivalent to or higher than HM₁₆₅S3B (SEQ ID NO: 827).

Example 12-4. Preparation of iCP-SOCS3 Recombinant Protein andInvestigation of Equivalence Thereof-4

In silico MHC class II binding analysis using iTope™ (ANTITOPE.LTD)revealed changing the V28 pl anchor residue in SDB (SEQ ID NO: 799)sequence to L makes this region human germline and as such both MHCclass II binding peptides within this region would be expected to be lowrisk due to T cell tolerance.

To prepare humanized SDB domain (SEQ ID NO: 804), iCP-SOCS3 was preparedas in FIG. 50. iCP-SOCS3 was prepared in the same manner as in Example6-1, except that SDB′ (SEQ ID NO: 804) having a substitution of valinewith leucine at an amino acid position 28 was used (FIG. 70). Further,protein purification was performed in the same manner as in example 6-2using the primer as below (Table 40).

TABLE 40 Recombinant Cargo SD Protein 5′ Primers 3′ primers SOCS3 — HS35′-GGAATTCCATATGGTCACCCAC 5′-CCCGGATCCTTAAA AGCAAGTTTCCCGCCGCC-3′GCGGGGCATCGTAC (SEQ ID NO: 955) TGGTCCAGGAA-3′ (SEQ ID NO: 956) —HM₁₆₅S3 5′-GGAATTCCATATGGCGCTGGCG 5′-CCCGGATCCTTAAAGTGCCGGTGGCGCTGGCGATT GCGGGGCATCGTAC GTGCCGGTCACCCACAGCAAGTGGTCCAGGAA-3′ TTTC-3′ (SEQ ID NO: 958) (SEQ ID NO: 957) A HM₁₆₅S3A5′-GGAATTCCATATGGCGCTGGCG 5′-CGCGTCGACTTACC GTGCCGGTGGCGCTGGCGATTTCGGCTGCACCGCC GTGCCGGTCACCCACAGCAAG ACGGCGATAC-3′TTTC-3′ (SEQ ID NO: 959) (SEQ ID NO: 960) B HM₁₆₅S3B5′-GGAATTCCATATGGCGCTGGCG 5′-CGCGTCGACTTAAA GTGCCGGTGGCGCTGGCGATTGGGTTTCCGAAGGC GTGCCGGTCACCCACAGCAAG TTGGCTATCTT-3′TTTC-3′ (SEQ ID NO: 961) (SEQ ID NO: 962) C HM₁₆₅S3C5′-GGAATTCCATATGGCGCTGGCG 5′-GCGTCGACTTAGGC GTGCCGGTGGCGCTGGCGATTCAGGTTAGCGTCGAG-3′ GTGCCGGTCACCCACAGCAAG (SEQ ID NO: 964)TTTC-3′ (SEQ ID NO: 963) D HM₁₆₅S3D 5′-GGAATTCCATATGGCGCTGGCG5′-GCGTCGACTTATTTT GTGCCGGTGGCGCTGGCGATT TTCTCGGACAGATA3′GTGCCGGTCACCCACAGCAAG (SEQ ID NO: 966) TTTC-3′ (SEQ ID NO: 965) EHM₁₆₅S3E 5′-GGAATTCCATATGGCGCTGGCG 5′-ACGCGTCGACTTAAGTGCCGGTGGCGCTGGCGATT CCTCCAATCTGTTC GTGCCGGTCACCCACAGCAAGGCGGTGAGCCTC-3′ TTTC-3′ (SEQ ID NO: 967) (SEQ ID NO: 968) B M₁₆₅S3B5′-GGAATTCCATATGGCGCTGGCG 5′-CGCGTCGACTTAAA GTGCCGGTGGCGCTGGCGATTGGGTTTCCGAAGGC GTGCCGGTCACCCACAGCAAG TTGGCTATCTT-3′  TTTC-3′ (SEQ ID NO: 977) (SEQ ID NO: 978)

As shown in FIG. 44, both HM₁₆₅S3B (SEQ ID NO: 827) and HM₁₆₅S3B′(V28L)were found to have high solubility/yield.

Example 12-5. Preparation of iCP-SOCS3 Recombinant Protein andInvestigation of Equivalence Thereof-5

In the same manner as in Example 7-1, RAW264.7 cells were treated withFITC-labeled HM₁₆₅S3B (SEQ ID NO: 827) and HM₁₆₅S313*(V28L) proteins,and cell permeability was evaluated.

As shown in FIG. 45, both HM₁₆₅S3B (SEQ ID NO: 827) and HM₁₆₅S313*(V28L)were found to have high cell permeability.

Example 12-6. Preparation of iCP-SOCS3 Recombinant Protein andInvestigation of Equivalence Thereof-6

To investigate biological activity equivalence of the HM₁₆₅S3B (SEQ IDNO: 827) and HM₁₆₅S313*(V28L) recombinant proteins, anti-proliferativeactivity was examined and induction of apoptosis of gastric carcinomacell line (AGS) was analyzed by Annexin V staining in the same manner asin Example 11-2, and inhibition of migration was analyzed in the samemanner as in Example 11-3.

Antiproliferative activity were evaluated with the CellTiter-Glo CellViability Assay. AGS cells (3×10³/well) were seeded in 96 well plates.The next day, cells were treated with DMEM (vehicle) or 10 μM HM₁₆₅S3B(SEQ ID NO: 827), HM₁₆₅S313*(V28L) for 96 hrs in the presence of serum(2%). Proteins were replaced daily. Cell growth and survival wereevaluated with the CellTiter-Glo Cell Viability Assay (Promega, Madison,Wis.). Measurements using a Luminometer (Turner Designs, Sunnyvale,Calif.) were conducted following the manufacturer's protocol.

It was confirmed that both HM₁₆₅S3B (SEQ ID NO: 827) andHM₁₆₅S313′(V28L) showed high anti-proliferative effects on gastriccarcinoma cells (FIG. 46), and also effects of inducing apoptosis (FIG.47) and of inhibiting migration of gastric carcinoma cells (FIG. 48),and in particular, HM₁₆₅S313′(V28L) exhibited efficacy equivalent to orhigher than HM₁₆₅S3B (SEQ ID NO: 827).

Example 12-7. Preparation of iCP-SOCS3 Recombinant Protein andInvestigation of Equivalence Thereof-7

iCP-SOCS3 of BS3M₁₆₅ (SEQ ID NO: 829) structure was prepared in the samemanner as in Example 6-1, and B′S3M₁₆₅ (SEQ ID NO: 831) iCP-SOCS3 wasalso prepared by humanized SDB domain (SEQ ID NO: 804) (FIG. 49a ).

TABLE 41 Recombinant Cargo SD Protein 5′ Primers 3′ primers SOCS3 BBS3M₁₆₅ 5′-GGAATTC 5′-ACGCGTCGACT CATAGATGGCAG TACGCCAGCGCCAAACAAAGCGAC-3′ CCGGCACCGCCAG (SEQ ID NO:  CGCAATCACCGGAA 973)GCGGGGCATCGTAC TGGTCCAG-3′ (SEQ ID NO: 974) B* B*S3M₁₆₅ 5′-GGAATTC5′-ACGCGTCGACT CATATGATGGCAGA TACGCCAGCGCCA ACAAAGCGAC-3′ CCGGCACCGCCAG(SEQ ID NO:  CGCAATCACCGGAA 975) GCGGGGCATCGTAC TGGTCCAG-3′(SEQ ID NO: 976)

Expressions and purifications of iCP-SOCS3 recombinant protein (BS3M₁₆₅(SEQ ID NO: 829), B′S3M₁₆₅ (SEQ ID NO: 831)) in E. coli (bottom) wereanalyzed in the same manner as in Examples 6-2 and 6-3, respectively,and shown in FIG. 49b . Further, E. coli codon-optimized iCP-SOCS3 wasprepared.

Example 13. Test of Biological Activity of iCP-SOCS3-Inhibition Activityof IFN-γ-Induced STAT Phosphorylation

Whether iCP-SOCS3 (HM₁₆₅S3B) (SEQ ID NO: 827) recombinant proteininhibits activation of the JAK/STAT-signaling pathway was examined bythe method of Example 8-1.

PANC-1 Cells (KCLB, Seoul, South Korea) were treated with 10 ng/ml IFN-γ(R&D systems, Abingdon, UK) for 15 min and treated with either DMEM(vehicle) or 1, 5, 10 μM aMTD/SD-fused SOCS3 recombinant proteins for 2hrs. The cells were lysed in RIPA lysis buffer (Biosesang, Seongnam,Korea) containing proteinase inhibitor cocktail (Roche, Indianapolis,Ind., USA), incubated for 15 min at 4° C., and centrifuged at 13,000 rpmfor 10 min at 4° C. Equal amounts of lysates were separated on 10%SDS-PAGE gels and transferred to a nitrocellulose membrane. Themembranes were blocked using 5% skim milk in TBST and for western blotanalysis incubated with the following antibodies: anti-phospho-STAT3(Cell Signaling Technology, USA), then HRP conjugated anti-rabbitsecondary antibody (Santacruz).

The well-known cytokine signaling inhibitory actions of SOCS3 areinflammation inhibition through i) inhibition of IFN-γ-mediated JAK/STATand ii) inhibition of LPS-mediated cytokine secretion. The ultimate testof cell-penetrating efficiency is a determination of intracellularactivity of SOCS3 proteins transported by aMTD. Since endogenous SOCS3are known to block phosphorylation of STAT3 by IFN-γ-mediated Januskinases (JAK) 1 and 2 activation, we demonstrated whether cell-permeableSOCS3 inhibits the phosphorylation of STATs. As shown in FIG. 50,iCP-SOCS3 (HM₁₆₅S3B) suppressed IFN-γ-induced phosphorylation of STAT3in dose dependent manner. In contrast, STAT phosphorylation was readilydetected in cells exposed to HS3B, which lacks the aMTD motif requiredfor membrane penetration, indicating that HS3B, which lacks an MTDsequence did not enter the cells, has no biological activity.

Example 14. Investigation of aMTD-Mediated Intracellular DeliveryMechanism

The mechanism of aMTD₁₆₅ (SEQ ID NO: 43)-mediated intracellular deliverywas investigated.

(1) RAW 264.7 cells were pretreated with 100 mM EDTA for 3 hours, andthen treated with 10 uM of iCP-SOCS3 (HM₁₆₅S3B) (SEQ ID NO: 827) proteinfor 1 hour, followed by flow cytometry in the same manner as in Example7-1 (FIG. 51A).

(2) RAW 264.7 cells were pretreated with 5 ug/ml of proteinase K for 10minutes, and then treated with 10 uM of iCP-SOCS3 (HM₁₆₅S3B) (SEQ ID NO:827) protein for 1 hour, followed by flow cytometry (FIG. 51B).

(3) RAW 264.7 cells were pretreated with 20 uM taxol for 30 minutes, andthen treated with 10 uM of iCP-SOCS3 (HM₁₆₅S3B) (SEQ ID NO: 827) proteinfor 1 hour, followed by flow cytometry (FIG. 52A).

(4) RAW 264.7 cells were pretreated with 1 mM ATP and 10 uM antimycinsingly or in combination for 2 hours, and then treated with 10 uM ofiCP-SOCS3 (HM₁₆₅S3B) (SEQ ID NO: 827) protein for 1 hour, followed byflow cytometry (FIG. 52B).

(5) RAW 264.7 cells were left at 4° C. and 37° C. for 1 hour,respectively, and then treated with 10 uM of iCP-SOCS3 (HM₁₆₅S3B) (SEQID NO: 827) protein for 1 hour, followed by flow cytometry (FIG. 53).

The aMTD-mediated intracellular delivery of SOCS3 protein did notrequire protease-sensitive protein domains displayed on the cell surface(FIG. 51B), microtubule function (FIG. 52A), or ATP utilization (FIG.52B), since aMTD₁₆₅ (SEQ ID NO: 43)-dependent uptake, compare to HS3 andHS3B, was essentially unaffected by treating cells with proteinase K,taxol, or the ATP depleting agent, antimycin. Conversely, iCP-SOCS3(HM₁₆₅S3B) (SEQ ID NO: 827) proteins uptake was blocked by treatmentwith EDTA and low temperature (FIGS. 51A and 53), indicating theimportance of membrane integrity and fluidity for aMTD-mediated proteintransduction.

Moreover, whether cells treated with iCP-SOCS3 (HM₁₆₅S3B) (SEQ ID NO:827) protein could transfer the protein to neighboring cells were alsotested.

For this, RAW 264.7 cells were treated with 10 uM of FITC-labelediCP-SOCS3 (HM₁₆₅S3B) (SEQ ID NO: 827) protein for 1 hour. Thereafter,these cells were co-cultured with PerCP-Cy5.5-CD14-stained RAW 264.7cells for 2 hours. Cell-to-cell protein transfer was assessed by flowcytometry, scoring for CD14/FITC double-positive cells. Efficientcell-to-cell transfer of HM₁₆₅S3B (SEQ ID NO: 827), but not HS3 or HS3B(FIG. 54), suggests that SOCS3 recombinant proteins containing aMTD₁₆₅(SEQ ID NO: 43) are capable of bidirectional passage across the plasmamembrane.

Example 14-2. Investigation of Basic CPP-Mediated Intracellular DeliveryMechanism

The mechanism of basic CPP (TAT and PolyR)-mediated intracellulardelivery was also investigated in the same manner as in Example 7-1 andExample 14.

As shown in FIG. 75, it was confirmed that aMTD165 (SEQ ID NO:43)/SD-fused SOCS3 recombinant proteins are independent to cell surfacereceptor (A) and the cell-permeability of aMTD165 (SEQ ID NO:43)/SD-fused SOCS3 recombinant proteins is not due to endocytosis (B).

Whether cells treated with aMTD165 (SEQ ID NO: 43)/SD-fused SOCS3,TAT/SD-fused SOCS3, and PolyR/SD-fused SOCS3 could transfer the proteinto neighboring cells were also tested on a molecular level in the samemanner as in Example 13.

For this, RAW 264.7 cells were treated with 5 μM of FITC-labeledHM₁₆₅S3B (SEQ ID NO: 827), HTS3B for 2 hour and washed with PBS twotimes. Thereafter, they were seeded on PANC-1 cell, incubated for 2hours and treated with 20 ng/ml of IFN-γ for 15 minutes, followed byWestern blotting in the same manner as in Example 8-1. And Cell-to-cellprotein transfer was assessed by flow cytometry.

As shown in FIG. 76, efficient cell-to-cell transfer of HM₁₆₅S3B (SEQ IDNO: 827), but not HTS3B or HRS3B, suggests that only SOCS3 recombinantproteins containing aMTD165 (SEQ ID NO: 43) are capable of bidirectionalpassage across the plasma membrane.

Moreover, as shown in FIG. 77, phospho-STAT3 was only reduced in cellstreated with HM₁₆₅S3B (SEQ ID NO: 827).

Example 15-1. Investigation of Time- and Dose-Dependency of iCP-SOCS3Cell-Permeability

It was examined whether the cell-permeability of iCP-SOCS3 recombinantprotein is dose-dependent. Cell-permeability of iCP-SOCS3 recombinantprotein was tested in the same manner as in Example 7-1 except for thecells being treated with 0.05 μM˜10 μM of iCP-SOCS3 recombinant proteinfor 1 hrs. As shown in FIGS. 78a and 78b , it was confirmed that thecell-permeability of iCP-SOCS3 recombinant protein is dose-dependent

Time-dependency of cell-permeability was also investigated. The cellswere treated with 10 μM of iCP-SOCS3 recombinant protein for 5˜180minutes. As shown in FIG. 79, high level of cell-permeability ofiCP-SOCS3 was observed only 5 minutes post-treatment and could be seeneven at 180 minutes mark.

Example 15-2. Investigation of Bioavailability of iCP-SOCS3

To investigate BA of the iCP-SOCS3 (HM₁₆₅S3B) (SEQ ID NO: 827)recombinant proteins, ICR mice (Doo-Yeol Biotech Co. Ltd., Seoul, Korea)were intravenous (IV) injected with 600 μg/head of 10 μM FITC(Fluorescein isothiocyanate, SIGMA, USA)-labeled SOCS3 recombinantproteins (HS3B, HM₁₆₅S3B (SEQ ID NO: 827)) and after 15 min, 30 min, 1H,2H, 4H, 8H, 12H, 16H, 24H, 36H, 48H, mice of each group were sacrificed.From the mice, peripheral blood mononuclear cells (PBMCs), splenocytes,and hepatocytes were separated.

Further, the spleen was removed and washed with PBS, and then placed ona dry ice and embedded in an O.C.T. compound (Sakura). Aftercryosectioning at 20 μm, tissue distributions offluorescence-labeled-SOCS3 proteins in different organs was analyzed byfluorescence microscopy (Carl Zeiss, Gottingen, Germany).

Isolation of PBMC

After anesthesia with ether, ophthalmectomy was performed and the bloodwas collected therefrom using a 1 ml syringe. The collected blood wasimmediately put in an EDTA tube and mixed well. The blood wascentrifuged at 4,000 rpm and 4° C. for 5 minutes, and plasma wasdiscarded and only buffy coat was collected in a new microtube. 0.5 mlof RBC lysis buffer (Sigma) was added thereto, followed by vortexing.The microtube was left at room temperature for 5 minutes, and thencentrifuged at 4,000 rpm and 4° C. for 5 minutes. 0.3 ml of PBS wasadded to a pellet, followed by pipetting and flow cytometry (FlowJocytometric analysis software, Guava, Millipore, Darmstadt, Germany).

Isolation of Splenocytes and Hepatocytes

Mice were laparotomized and the spleen or liver were removed. The spleenor liver thus removed was separated into single cells using a CellStrainer (SPL, Korea). These cells were collected in a microtube,followed by centrifugation at 4,000 rpm and 4° C. for 5 minutes. 0.5 mlof RBC lysis buffer was added thereto, followed by vortexing. Themicrotube was left at room temperature for 5 minutes, and thencentrifuged at 4,000 rpm and 4° C. for 5 minutes. 0.5 ml of PBS wasadded to a pellet, followed by pipetting and flow cytometry (FlowJocytometric analysis software, Guava, Millipore, Darmstadt, Germany).

As shown in FIG. 55, in PBMCs, the maximum permeability of iCP-SOCS3 wasobserved at 30 minutes, and in splenocytes, the maximum permeability ofiCP-SOCS3 was observed at 2 hours and maintained up to 16 hours. Inhepatocytes, the maximum permeability of iCP-SOCS3 was observed at 15minutes and maintained up to 16 hours.

Example 15-3. Investigation of Bio-Distribution of iCP-SOCS3 RecombinantProtein

To investigate BA of the iCP-SOCS3 (HM₁₆₅S3B) (SEQ ID NO: 827)recombinant proteins, ICR mice (Doo-Yeol Biotech Co. Ltd., Seoul, Korea)were intravenous (IV) injected with 600 μg/head of 10 μM FITC(Fluorescein isothiocyanate, SIGMA, USA)-labeled SOCS3 proteins (HS3B,HM₁₆₅S3B) and after 2H, 8H, 12H, 24H, mice of each group weresacrificed. From the mice, pancreas was removed and washed with PBS, andthen placed on a dry ice and embedded in an O.C.T. compound (Sakura).After cryosectioning at 20 μm, tissue distributions offluorescence-labeled-SOCS3 proteins in pancreas tissue was analyzed byfluorescence microscopy (Carl Zeiss, Gottingen, Germany) (FIG. 56).

As shown in FIG. 56, in the pancreas tissue, very high distribution ofiCP-SOCS3 was observed at 2 hours, and maintained up to 8 hours.Therefore, it can be seen that iCP-SOCS3 is rapidly delivered from bloodto various tissues within 2 hours, and maintained up to 8-16 hoursdepending on the tissues.

Example 16-1. Investigation of Tumor Targeting of iCP-SOCS3

Tumor-targeting of the anticancer agent iCP-SOCS3 (HM₁₆₅S3B) (SEQ ID NO:827) recombinant proteins was investigated in an orthotopic xenograftmodel. The protocol was adapted from World J Gastroenterol. 2014 Jul.28; 20(28): 9476-9485. To generate an orthotopic xenograft mouse model,PANC-1-Luc cells (1×10⁶ cells/mouse) cultured in RPMI 1640 (Hyclone,Logan, Utah, USA) containing 10% FBS were mixed with Matrigel at a 1:1ratio and 200 μl of the mixture were injected into pancreas tail offemale Balb/e^(nu/nu) mice (5-week-old, Doo-Yeol Biotech Co. Ltd.Korea). After 7 weeks, the mice were IP injected with 100 μl ofluciferin (Sigma-Aldrich, St. Louis, Mo., USA) and imaged by XenogenIVIS-200 imaging system 15 minute later. As shown in FIG. 80 (A),pancreatic cancer model was well established.

The iCP-SOCS3 recombinant proteins were labeled and IV injected to themice with 40 mg/kg according to a manual of Alexa680 kit (Thermo FisherScientific, San Jose, Calif., USA). As shown in FIG. 80 (B), theiCP-SOCS3 recombinant proteins were delivered to every part of the bodyat 5 minutes and more iCP-SOCS3 recombinant proteins were accumulated inpancreas. As time passed, a large amount of the proteins wereaccumulated only in tumor. Therefore, the iCP-SOCS3 recombinant proteinwas proved to be a mechanism-specific anticancer agent that specificallytargets cancer tissues.

Example 16-2. Investigation of Anticancer Efficacy of iCP-SOCS3Recombinant Protein

In order to develop the iCP-SOCS3 recombinant protein as a therapeuticagent for pancreatic cancer, its permeability to pancreatic cancer celllines and pancreas tissues was investigated.

PANC-1 cells (KCLB, Seoul, South Korea) were seeded in a 12 well plate,grown to 90% confluence. Cells were treated with 10 μM FITC-labelediCP-SOCS3 recombinant proteins and cultivated for 1 hr at 37° C.

After cultivation, the cells were treated with proteinase K (10 μg/mL,SIGMA, USA) and washed three times with ice-cold PBS (Phosphate-bufferedsaline, Hyclone, USA) to remove surface-bound proteins, and internalizedproteins were measured by flow cytometry (FlowJo cytometric analysissoftware, Guava, Millipore, Darmstadt, Germany). Untreated cells (gray)and equimolar concentration of unconjugated FITC (FITC only,green)-treated cells were served as control (FIG. 57).

Pancreas tissue permeability was examined at 2, 8, 12, and 24 hoursafter protein injection as described in Example 7-2.

As shown in FIG. 57, iCP-SOCS3 recombinant proteins (FITC-HM₁₆₅S3SB (SEQID NO: 827)) promoted the transduction into cultured PANC-1 cells. Incontrast, SOCS3 proteins containing non-aMTD (FITC-HS3 and FITC-HS3B)did not appear to enter cells.

Therefore, these data indicate that iCP-SOCS3 protein could beintracellularly delivered and distributed to the pancreatic cancer cellsand pancreas tissue, contributing for beneficial biotherapeutic effects.

Example 16-3. Investigation of Anticancer Efficacy of iCP-SOCS3Recombinant Protein

To develop the iCP-SOCS3 recombinant protein as a mechanism-specifictherapeutic agent for pancreatic cancer, SOCS3 levels endogenouslyexpressed and activation of JAK/STAT-signaling pathway were investigatedin different pancreatic cancer cell lines, normal cells, and normalhepatic cells (ATCC, Manassas, Va., USA & KCLB, Seoul, South Korea).

Example 16-3-1. Analysis of Hypermethylation Level in Cell Line

SOCS3 expression is suppressed due to methylation in cancer cells, andtherefore, inflammation or cancer development is increased. Further, toinvestigate the effect of cell permeable SOCS3, a cancer cell line whereendogenous SOCS3 expression is suppressed should be selected or appliedto a model, and therefore, the present experiments were performed.

Genomic DNA was extracted from cancer cell line using an Exgene™ TissueSV mini kit (Geneall®, Korea). DNA was quantified, and experiments wereperformed using 500 ng of gDNA and an EZ DNA Methylation-Gold™ kit (ZYMOResearch, Orange, Calif., USA) according to the manufacturer'sinstructions. DNA was used to perform PCR, and methylation andunmethylation of endogenous SOCS3 were qualitatively analyzed byelectrophoresis. In this regard, the primers used are as follows.

Unmethyl-F was 5′-tag tgt gta agt tgt agg aga gtg g-3′(SEQ ID NO: 816),Unmethyl-R was 5′-cta aac ata aaa aaa taa cac taa tcc aaa-3′ (SEQ ID NO:817), Methyl-F was 5′-gta gtg cgt aag ttg tag gag agc-3′ (SEQ ID NO:818), and Methyl-R was 5′-gta aaa aaa taa cgc taa tcc gaa-3′ (SEQ ID NO:819). PCR was performed for 30 cycles consisting of pre-denaturation at95° C. for 5 minutes, denaturation at 95° C. for 30 seconds, annealingat 60° C. for 45 seconds, and extension at 72° C. for 1 minute, and thenfinal extension at 72° C. for 8 minutes.

As shown in FIG. 81, unmethylation of SOCS3 was observed in H6c7 cellswhich are normal pancreatic cells, whereas hypermethylation of thepromoter region of SOCS3 gene was observed in BxPC3, Capan2, HPAC andPANC-1 which are pancreatic cancer cell lines (U: unmethylated SOCS3, M:methylated SOCS3). These results indicate that SOCS3 is silenced byhypermethylation in pancreatic cancer cell lines.

Example 16-3-2. Analysis of Expression Level of Endogenous SOCS3 mRNA inPancreatic Cancer Cell Line

SOCS3 mRNA expression levels in cancer cells were analyzed by RT-PCR.mRNAs were isolated from normal cell lines and cancer cell linesaccording to a method provided in a manufacturer's sheet of Hybrid-R(Geneall, Korea), and PCR was performed using SOCS3 primer F 5′-cct actgaa ccc tcc tcc ga-3′ (SEQ ID NO: 820) and SOCS3 primer R 5′-gca get gggtga ctt tct ca-3′ (SEQ ID NO: 821) for 30 cycles consisting ofdenaturing (95° C.), annealing (60° C.), and extending (72° C.) for 45seconds each.

It was confirmed that high expression levels of SOCS3 were observed inthe normal HEK293 and Chang cells whereas low expression levels of SOCS3were observed in the pancreatic cancer cell lines (Capan2, HPAC) (FIG.82).

Example 16-3-3. Analysis of Endogenous SOCS3 and JAK/STAT SignalingActivation Status in Pancreatic Cancer Cell Line

JAK/STAT3 activation in cancer cells was analyzed by Western blotanalysis. The normal HEK293 cells, and pancreatic cancer cell lines,BxPC-3, Capan2, HPAC and PANC-1 were washed with PBS, and then the cellswere lysed in RIM lysis buffer (Biosesang, Seongnam, Korea) containingproteinase inhibitor cocktail (Roche, Indianapolis, Ind., USA),incubated for 15 min at 4° C., and centrifuged at 13,000 rpm for 10 minat 4° C. to isolate proteins, followed by western blotting in the samemanner as in Example 8-1.

As shown in FIG. 83, low JAK1 and JAK2 phosphorylations and STAT1 andSTAT3 phosphorylations were observed in normal HEK293 cells, whereas lowexpression levels of SOCS3 were observed in the pancreatic cancer celllines, BxPC3, Capan2, HPAC and PANC1. These results suggest apossibility of developing a mechanism-specific anticancer agent, becauseSOCS3-deficient cancer cells can be replenished with cell permeableproteins, and activated JAK/STAT-signaling can be negatively regulated.

Example 17. Investigation of Anticancer Efficacy (Anti-ProliferativeActivity) of iCP-SOCS3 Recombinant Protein

To develop the iCP-SOCS3 recombinant protein as a mechanism-specifictherapeutic agent for pancreatic cancer, efficacy of iCP-SOCS3 onproliferation of pancreatic cancer cell lines was investigated.

Cells originated from human pancreatic cancer cell (PANC-1, BxPC amdHPAC) and mouse fibroblast (NIH3T3) were purchased from ATCC (Manassas,Va.) and KCLB (Seoul, Korea) and maintained as recommended by thesupplier. These cells (3×10³/well) were seeded in 96 well plates. Thenext day, cells were treated with DMEM (vehicle) or 10 μM recombinantSOCS3 proteins for 96 hrs in the presence of serum (2%). Proteins werereplaced daily. Cell growth and survival were evaluated with theCellTiter-Glo Cell Viability Assay (Promega, Madison, Wis.).Measurements using a Luminometer (Turner Designs, Sunnyvale, Calif.)were conducted following the manufacturer's protocol.

Since the endogenous level of SOCS3 protein is reduced in pancreaticcancer patient, and SOCS3 negatively regulates cell growth and motilityin cultured pancreatic cancer cells, we investigated whether iCP-SOCS3inhibits cell viability through SOCS3 intracellular replacement inpancreatic cancer cells. As shown in FIG. 58, SOCS3 recombinant proteinscontaining aMTD₁₆₅ (SEQ ID NO: 43) significantly suppressed cancer cellproliferation. HM₁₆₅S3B (SEQ ID NO: 827) (iCP-SOCS3) protein was themost cytotoxic to pancreatic cancer cells—over 90% in 10 μM treatment(p<0.01)—especially compared to vehicle alone (i.e. exposure of cells toculture media without recombinant proteins; left). However, neithercell-permeable SOCS3 protein adversely affected the cell viability ofnon-cancer cells (NIH3T3) even after exposing these cells to equalconcentrations (10 μM) of protein over 4 days. These results suggestthat the iCP-SOCS3 protein is not overly toxic to normal cells andselectively kills tumor cells, and would have a great ability to inhibitcell survival-associated phenotypes in pancreatic cancer without anysevere aberrant effects as a protein-based biotherapeutics.

Example 18. Investigation of Anti-Cancer Efficacy (Cell MigrationInhibition) of iCP-SOCS3

In order to develop the iCP-SOCS3 recombinant protein as a therapeuticagent for pancreatic cancer, efficacy of iCP-SOCS3 on migration andmetastasis of pancreatic cancer cell lines was investigated.

Cells were seeded into 12-well plates, grown to 90% confluence, andincubated with 10 μM HS3B (Non-CP-SOCS3), HM165S3B (iCP-SOCS3)(SEQ IDNO: 827) in serum-free medium for 2 hrs prior to changing the growthmedium (recommended medium by the supplier). The cells were washed twicewith PBS, and the monolayer at the center of the well was “wounded” byscraping with a pipette tip. Cells were cultured for an additional 24-48hrs and cell migration was observed by phase contrast microscopy (Nikon,ECLIPSE Ts2). The migration was quantified by counting the number ofcells that migrated from the wound edge into the clear area.

As shown in FIG. 59b , HM₁₆₅S3B protein (iCP-SOCS3) (SEQ ID NO: 827)suppressed the repopulation of wounded monolayer although SOCS3 proteinlacking aMTD₁₆₅ (SEQ ID NO: 43) (HS3B) had no effect on the cellmigration, in PANC-1 cells. Further, a reference drug, gemcitabine (1˜10μM) which is used for the standard regimen of pancreatic cancer did notaffect PANC-1 cell migration. In normal cells (NIH3T3), HM165S3B protein(iCP-SOCS3) (SEQ ID NO: 827) had no effect on the cell migration.

Transwell Migration Assay

The lower surface of Transwell inserts (Costar) was coated with 0.1%gelatin, and the membranes were allowed to dry for 1 hr at roomtemperature. The Transwell inserts were assembled into a 24-well plate,and the lower chamber was filled with growth media containing 10% FBSand FGF2 (40 ng/ml). Cells were added to each upper chamber at a densityof 5×10⁵, and the plate was incubated at 37° C. in a 5% CO₂ incubatorfor 24 hrs. Migrated cells were stained with 0.6% hematoxylin and 0.5%eosin and counted.

As shown in FIG. 60, PANC-1 cells treated with HM₁₆₅S3B recombinantprotein (iCP-SOCS3) (SEQ ID NO: 827) also showed significant inhibitoryeffect on their Transwell migration compared with untreated cells(Vehicle) and non-permeable SOCS3 protein-treated cells.

Invasion Assay

The lower surface of Transwell inserts (Costar) was coated with 0.1%gelatin, the upper surface of Transwell inserts was coated with matrigel(40 μg per well; BD Pharmingen, San Diego, Calif., USA), and themembranes were allowed to dry for 1 hr at room temperature. TheTranswell inserts were assembled into a 24-well plate, and the lowerchamber was filled with growth media containing 10% FBS and FGF2 (40ng/ml). Cells (5×10⁵) were added to each upper chamber, and the platewas incubated at 37° C. in a 5% CO₂ incubator for 24 hrs. Migrated cellswere stained with 0.6% hematoxylin and 0.5% eosin and counted.

As shown in FIG. 61, PANC-1 cells treated with HM₁₆₅S3B recombinantprotein (iCP-SOCS3) (SEQ ID NO: 827) caused remarkable decrease ininvasion compared with the control proteins. Taken together, these dataindicate that iCP-SOCS3 contributes to inhibit tumorigenic activities ofpancreatic cancer cells.

Example 19-1. Investigation of Anti-Cancer Efficacy (Induction ofApoptosis) of iCP-SOCS3

To further determine the effect of iCP-SOCS3 on the tumorigenicity ofpancreatic cancers, we subsequently investigated whether iCP-SOCS3regulates apoptosis in PANC-1 cells.

Annexin V/7-Aminoactinomycin D (7-AAD) staining was performed using flowcytometry according to the manufacturer's guidelines (BD Pharmingen, SanDiego, Calif., USA). Briefly, 1×10⁶ cells were washed three times withice-cold PBS. The cells were then resuspended in 100 μl of bindingbuffer and incubated with 1 μl of 7-AAD and 1 μl of Annexin V-7-AAD for30 min in the dark at 4° C. Flow cytometric analysis was immediatelyperformed using a guava easyCyte™ 8 Instrument (Merck Millipore,Darmstadt, Germany). In this regard, gemcitabine (0.1-10 μM) was used asa reference drug.

As shown in FIG. 62, 10 uM HM₁₆₅S3B protein (iCP-SOCS3) (SEQ ID NO: 827)was efficient inducer of apoptosis in PANC-1 cells, as assessed byAnnexin V staining. Consistently, no changes in Annexin V staining wereobserved in PANC-1 cells treated with HS3B compared to untreated cell(Vehicle). The reference drug, gemcitabine less effectively inducedapoptosis of PANC-1 cells than iCP-SOCS3.

Example 19-2. Investigation of Anti-Cancer Efficacy (Inhibition of CellCycle Progression) of iCP-SOCS3

To further determine the effect of iCP-SOCS3 on the tumorigenicity ofpancreatic cancer cells, we subsequently investigated whether iCP-SOCS3regulates cell cycle progression in pancreatic cancer cells (PANC-1).

PANC-1 and normal cells were treated with 10 uM protein (Non-CP-SOCS3(HS3B) and iCP-SOSC3 (HM₁₆₅S3B) (SEQ ID NO: 827)) for 8 hrs. After thetreatment, cells were washed twice with cold PBS and re-suspended in 1ml cold PBS, fixed in cold 70% ethanol, washed with cold PBS twice andre-suspended in PI master mix (PI 10 ug/ml (sigma), 50 ug/ml RNase A(invitrogen) in staining buffer) at a final cell density of 2×10⁵cell/ml. The cell mixtures were incubated 30 min in the dark at 4° C.Flow cytometric analysis was immediately performed using a guavaeasyCyte™ 8 Instrument (Merck Millipore, Darmstadt, Germany).Gemcitabine (0.1-10 μM) was used for reference drug.

HM₁₆₅S3B protein (iCP-SOCS3)(SEQ ID NO: 827) efficiently inhibited cellcycle progression in PANC-1 (FIG. 63b ), while HM₁₆₅S3B protein(iCP-SOCS3) (SEQ ID NO: 827) did not inhibit cell cycle progression innormal cells (FIG. 63a ). Further, the reference drug, gemcitabinehardly increased the amount of subG1.

Gemcitabine which is clinically used as a therapeutic agent forpancreatic cancer at present is a small molecule, and its molecularweight is 100 times smaller than that of iCP-SOCS3 which is a newbio-drug made of protein.

However, when gemcitabine and iCP-SOCS3 were treated at the sameconcentration, induction efficiency of cancer cell apoptosis ofgemcitabine was reduced to ⅓, compared to that of iCP-SOCS3, indicatingthat gemcitabine shows considerably lower anti-cancer efficacy thaniCP-SOCS3, considering their molar ratio.

Example 19-3. Investigation of Anti-Cancer Efficacy (Induction ofApoptosis) of iCP-SOCS3

To further determine the effect of iCP-SOCS3 on the tumorigenicity ofPANC-1, we subsequently investigated whether iCP-SOCS3 regulatesapoptosis in PANC-1.

Apoptotic cells were analyzed using terminal dUTP nick-end labeling(TUNEL) assay with In Situ Cell Death Detection kit TMR red (Roche, 4056Basel, Switzerland). Cells were treated for 24 hr with 10 μM HS3B orHM₁₆₅S3B (SEQ ID NO: 827) proteins with 2% fetal bovine serum andapoptotic cells were visualized by TUNEL staining. Treated cells werewashed with cold PBS two times, fixed in 4% paraformaldehyde (PFA,Junsei, Tokyo, Japan) for 1 hr at room temperature, and incubated in0.1% Triton X-100 for 2 min on the ice. Cells were washed with cold PBStwice, and treated TUNEL reaction mixture for 1 hr at 37° C. in dark,washed cold PBS three times and observed by fluorescence microscopy(Nikon, Tokyo, Japan).

HM₁₆₅S3B protein (iCP-SOCS3) (SEQ ID NO: 827) was considerably efficientinducer of apoptosis in PANC-1 cells (FIG. 64), as assessed by afluorescent terminal dUTP nick-end labeling (TUNEL) assay. Consistently,no changes in TUNEL was observed in PANC-1 cells treated with HS3Bcompared to untreated cell (Vehicle).

Example 20. Investigation of Anti-Cancer Efficacy (Effect on MolecularMechanism-Mode of Action) of iCP-SOCS3

To further determine the effect of iCP-SOCS3 on the tumorigenicity ofPANC1, we subsequently investigated whether iCP-SOCS3 regulatesapoptosis in PANC-1 cells.

Cells were treated for 2 hr, 3 hr or 24 hr with 10 μM HS3B or HM₁₆₅S3B(SEQ ID NO: 827) proteins, lysed in RIPA lysis buffer containingproteinase inhibitor cocktail, incubated for 15 min at 4° C., andcentrifuged at 13,000 rpm for 10 min at 4° C. Equal amounts of lysateswere separated on 15% SDS-PAGE gels and transferred to a nitrocellulosemembrane. The membranes were blocked using 5% skim milk or 5% Albumin inTBST and incubated with the following antibodies: anti-Bc1-2 (Santa Cruzbiotechnology), anti-Cleaved Caspase 3 (Cell Signaling Technology),anti-phospho-p53 and anti-p53 (Cell Signaling Technology), anti-p21(Santa Cruz biotechnology) then HRP conjugated anti-mouse or anti-rabbitsecondary antibody. The expression of each protein was determined byimmunoblotting with indicated antibodies. An antibody against β-actinwas used as a loading control.

PANC-1 cells treated with HM₁₆₅S3B protein (iCP-SOCS3)(SEQ ID NO: 827)dramatically reduced the expression of anti-apoptotic protein such asB-cell lymphoma 2 (Bc1-2), phospho-Rb (cell cycle-related protein) andincreased the level of cleaved cysteine-aspartic acid protease(caspase-3) at 24 hr (FIG. 65). In addition, PANC-1 cells treated withHM₁₆₅S3B protein (iCP-SOCS3) (SEQ ID NO: 827) dramatically increased thelevel of phospho-p53 and p21 at 3 hr. Furthermore, iCP-SOCS3 inhibitedactivated JAK/STAT pathway at 2 hr and 24 hr in PANC-1 cells (FIG. 65).

BxPC-3 cells treated with HM₁₆₅S3B protein (iCP-SOCS3)(SEQ ID NO: 827)significantly reduced the expression of phospho-STAT1 and phosphor-STATSat 2 hr (FIG. 84). In addition, BxPC-3 cells treated with HM₁₆₅S3Bprotein (iCP-SOCS3)(SEQ ID NO: 827) dramatically reduced the level ofphosphor-Rb and VEGF at 24 hr (FIG. 84).

These results indicate that iCP-SOCS3 induces apoptosis of pancreaticcancer cells and may suppress the cancer progression by this pathway.

Example 21. Investigation of Anti-Cancer Efficacy of iCP-SOCS3 inXenograft Model (Cell-Derived Xenograft (CDX)-Model)

In the following experiments, western blotting was performed in the samemanner as in Example 8-1, and RT-PCR and IHC were performed by thefollowing method.

RT-PCR

Tumor tissues were finely minced using a homogenizer according to themanufacturer's protocol of Hybrid-R (geneall, Korea), and then mRNA wasisolated therefrom. 1 ug of mRNA thus separated was used to synthesizecDNA using an Accupower RT Premix (Bioneer, Korea). PCR was performedusing 2 ul of cDNA and the primers of Table 45. PCR was performed usingan Accupower PCR Premix (Bioneer, Korea) for 30 cycles consisting ofdenaturing (95° C.), annealing (60° C.), and extending (72° C.) for 45sec each.

Genes Forward Sequence Reverse Sequence Cyclin E CCGTTTACAAGCTAAGCAGCGTGGTTCCAAGTCAGAATGC (SEQ ID NO: 839) (SEQ ID NO: 840) CDK4CTATGGGACAGTGTACAAGG GTCACCAGAATGTTCTCTGG (SEQ ID NO: 841)(SEQ ID NO: 842) PCNA GAGGCCTGCTGGGATATTAG TACGTGCAAATTCACCAGAAG(SEQ ID NO: 843) (SEQ ID NO: 844) Bax CCCTTTTGCTTCAGGGTTTCGCCACTCGGAAAAAGACCTC (SEQ ID NO: 845) (SEQ ID NO: 846) p53AGGTGCATGTTTGTGCCTGT TCAGCTCTCGGAACATCTCG (SEQ ID NO: 847)(SEQ ID NO: 848) p21 CAGCGGAACAAGGAGTCAGA AGAAACGGGAACCAGGACAC(SEQ ID NO: 849) (SEQ ID NO: 850) FAK TGGTGAAAGCTGTCATCGAGCTGGGCCAGTTTCATCTTGT (SEQ ID NO: 851) (SEQ ID NO: 852) VEGFCTTCAAGCCATCCTGTGTGC ACGCGAGTCTGTGTTTTTGC (SEQ ID NO: 863)(SEQ ID NO: 854) Glypican-1 TCATCACCGACAAGTTCTGG TGGTCATGATCTTCAGCTGC(SEQ ID NO: 855) (SEQ ID NO: 856) MUC1 GTTACAGGTTCTGGTCATGCGATTGAACTGTGTCTCCACT (SEQ ID NO: 857) (SEQ ID NO: 858) MUC3ATTCCTAGCACACATTCCTCC GAAAGCCTGTGATAGAAGCG (SEQ ID NO: 859)(SEQ ID NO: 860) MUC6 AGTACATGGGTCAGATGTGC GCATAACTGCTGTTCTGTGG(SEQ ID NO: 861) (SEQ ID NO: 862) GAPDH AAGGGTCATCATCTCTGCCCGTGATGGCATGGACTGTGGT (SEQ ID NO: 863) (SEQ ID NO: 864)

Immunohistochemistry (IHC)

Tissue samples were fixed in 4% Paraformaldehyde (Duksan, South Korea)for 3 days, dehydrated, cleared with xylene and embedded in Paraplast.Sections (6 μm thick) of tumor were placed onto poly-L-lysine coatedslides. To block endogenous peroxidase activity, sections were incubatedfor 15 min with 3% H₂O₂ in methanol. After washing three times with PBS,slides were incubated for 30 min with blocking solution (5% fetal bovineserum in PBS). Mouse anti-Bax antibody (sc-7480, Santa CruzBiotechnology, SantaCruz, Calif., USA) and rabbit anti-VEGF (ab46154,Abcam, Cambridge, UK) were diluted 1:1000 (to protein concentration 0.1μg/ml) in blocking solution, applied to sections, and incubated at 4° C.for 24 hrs. After washing three times with PBS, sections were incubatedwith biotinylated mouse and rabbit IgG (Vector Laboratories, Burlingame,Calif., USA) at a 1:1000 dilution for 1 hr at room temperature, thenincubated with avidin-biotinylated peroxidase complex using aVectorstain ABC Kit (Vector Laboratories, Burlingame, Calif., USA) for30 min at room temperature. After the slides are reacted with oxidized3, 3-diaminobenzidine as a chromogen, they were counterstained withHarris hematoxylin (Sigma-Aldrich, USA). Permanently mounted slides wereobserved and photographed using a microscope equipped with a digitalimaging system (ECLIPSE Ti, Nikon, Japan).

Anti-tumor activity of iCP-SOCS3 against human cancer xenografts wasassessed. Female Balb/c″^(nu/nu) mice (5-week-old, Doo-Yeol Biotech Co.Ltd. Korea) were subcutaneously implanted with PANC-1 tumor block (1mm³) into the left back side of the mouse. Tumor-bearing mice wereintravenously administered with 600 μg/head of iCP-SOCS3 (HM₁₆₅S3B) (SEQID NO: 827) or the control proteins (HS3B) for 21 days and observed for3 weeks following the termination of the treatment. After proteintreatment, mice were killed, and six organs (brain, heart, lung, liver,kidney, and spleen) from each were collected and kept in a suitablefixation solution until the next step. Tumor size was monitored bymeasuring the longest (length) and shortest dimensions (width) once aday with a dial caliper, and tumor volume was calculated aswidth2×length×0.5.

As shown in FIGS. 66a and 66b , HM₁₆₅S3B (SEQ ID NO: 827) proteinsignificantly suppressed the tumor growth (p<0.05) during the treatmentand the effect persisted for at least 2 weeks after the treatment wasterminated (85% at day 42, respectively). Whereas, the growth ofHS3B-treated tumors increased, matching the rates observed in controlmice (Vehicle). These results suggest that iCP-SOCS3 inhibits the growthof established tumors as well as the tumor growth of pancreatic cancercells.

mRNA levels were analyzed using the tumor tissues removed at 3 weeks(Day 42) after termination of drug administration to analyze changes inexpressions of major biomarker genes by iCP-SOCS3.

Expressions of apoptosis (Bax), cell cycle (p21, p53, CDK4, cyclinE),proliferation (PCNA), angiogenesis (VEGF), and migration (FAK)-relatedgenes were significantly regulated by treatment of iCP-SOCS3. Further,expressions of Glypican-1, MUC1, and MUC3A which are biomarker genes ofpancreatic cancer were remarkably reduced by iCP-SOCS3.

Accordingly, it was confirmed that iCP-SOCS3 is able to inhibitdevelopment of pancreatic cancer through expressions of various factorsregulating cancer activity such as apoptosis, cell cycle, proliferation,migration, angiogenesis, etc., indicating characteristics of iCP-SOCS3as a mechanism-specific therapeutic agent targeting pancreatic cancer(FIG. 67).

Further, tumor tissues removed at 3 weeks (Day 42) after termination ofdrug administration were used to perform western blotting and IHC, andincrease or decrease in the expressions of cancer activity regulatingfactors by iCP-SOCS3 was observed (FIG. 68, FIGS. 85 and 69).

In iCP-SOCS3-treated tumor tissues, expressions of a cell cycleregulator p21 and an apoptosis inducer Bax were increased, andexpression of an apoptosis suppressor Bcl-2 was decreased. Further,expression of an angiogenesis inducer VEGF, angiopoietin-I (Angt I) werealso remarkably decreased (FIG. 68, FIGS. 85 and 69).

Accordingly, it was demonstrated that cell cycle, apoptosis, andangiogenesis are also regulated by iCP-SOCS3 in vivo to inhibit canceractivity.

Example 22. Investigation of Anti-Cancer Efficacy of iCP-SOCS3 inOrthotopic Xenograft Model

Anti-cancer efficacy of the iCP-SOCS3 (HM₁₆₅S3B)(SEQ ID NO: 827)recombinant protein was investigated in an orthotopic xenograft model.To generate a pancreatic cancer orthotopic xenograft mouse model,BxPC-3-Luc cells (1×10⁶/mouse) cultured in RPMI 1640 (Hyclone, Logan,Utah, USA) containing 10% FBS were mixed with Matrigel at a 1:1 ratioand 200 μl of the mixture were injected into pancreas tail of maleBalb/c^(nu/nu) mice (6-week-old, Doo-Yeol Biotech Co. Ltd. Korea). After12 days, the mice were IP injected with 100 μl of luciferin(Sigma-Aldrich, St. Louis, Mo., USA) and imaged by Xenogen IVIS-200imaging system 15 minute later. It was confirmed that the pancreaticcancer model was well established, and then the mice were divided into 4groups (5 mice per group) based on ROI value (FIG. 86a ).

Two groups of mice were IV injected with iCP-SOCS3 recombinant proteins,3 mg/kg and 30 mg/kg respectively, for 4 weeks (five times per week).And a group of mice were single IV injected with 50 mg/kg of a referencedrug, Gemcitabine (Gemzar®). Tumor growth was monitored by IVIS afterfour weeks, and mice were sacrificed to extract tumor.

In the following experiments, western blotting was performed in the samemanner as in example 8-1.

The iCP-SOCS3 recombinant proteins showed high anticancer efficacy whentreated with 30 mg/kg to the mice. As shown in the result of the westernblot analysis (FIG. 86b ), phospho-STAT1 and phospho-STAT3 wereinhibited in the group treated with iCP-SOCS3 recombinant proteins.Moreover, expression level of VEGF, which is an angiogenesis marker, wasalso reduced.

Example 23. Investigation of Anti-Cancer Efficacy of iCP-SOCS3 inXenograft Model (Cell-Derived Xenograft (CDX)-Model) in Dose-DependentManner

BxPC-3 CDX model was constructed in the same manner as in example 21,and the anticancer efficacy of iCP-SOCS3 was investigated. The iCP-SOCS3recombinant proteins inhibited tumor growth in a dose-dependent manner(FIGS. 87a and 87b ). The results show that the iCP-SOCS3 recombinantproteins have a superior anticancer effect in pancreatic cancer.

Statistical Analysis

Statistical analysis and graphic presentation have been performed usingGraphPad Prism 5.01 software (GraphPad, La Jolla, Calif., USA). Allexperimental data are presented as means±SEM. Statistical significancewas analyzed by the Student's t-test or ANOVA method. Experimentaldifferences between groups were assessed using paired Student's t-tests.For animal experiments, ANOVA was used for comparing between and withingroups to determine the significance. Differences with p<0.05 areconsidered to be statistically significant.

Those skilled in the art to which the present invention pertains willappreciate that the present invention may be implemented in differentforms without departing from the essential characteristics thereof.Therefore, it should be understood that the disclosed embodiments arenot limitative, but illustrative in all aspects. The scope of thepresent invention is made to the appended claims rather than to theforegoing description, and all variations which come within the range ofequivalency of the claims are therefore intended to be embraced therein.

The invention claimed is:
 1. An improved Cell-Permeable (iCP)-SOCS3recombinant protein comprising a SOCS3 protein, an advancedmacromolecule transduction domain (aMTD) and a solubilization domain(SD), wherein the iCP-SOCS3 recombinant protein is represented byfollowing structural formula:A-B-C, wherein A is the aMTD, B is the SOCS3 protein, and C is the SD,wherein the aMTD has the amino acid sequence of SEQ ID NO: 122, and theSD has an amino acid sequence independently selected from the groupconsisting of SEQ ID NOs: 798, 799, 800, 801, 802, 803, and
 804. 2. TheiCP-SOCS3 recombinant protein according to claim 1, wherein the SOCS3protein has the amino acid sequence of SEQ ID NO:
 814. 3. The iCP-SOCS3recombinant protein according to claim 2, wherein the SOCS3 protein isencoded by the polynucleotide sequence of SEQ ID NO:
 815. 4. TheiCP-SOCS3 recombinant protein according to claim 1, wherein the aMTD isencoded by the polynucleotide sequence of SEQ ID NO:
 362. 5. TheiCP-SOCS3 recombinant protein of claim 1, wherein the SD is encoded by apolynucleotide sequence independently selected from the group consistingof SEQ ID NOs: 805, 806, 807, 808, 809, 810, and
 811. 6. The iCP-SOCS3recombinant protein according to claim 1, wherein the iCP-SOCS3recombinant protein has a histidine-tag affinity domain additionallyfused to one end thereof.
 7. The iCP-SOCS3 recombinant protein accordingto claim 6, wherein the histidine-tag affinity domain has the amino acidsequence of SEQ ID NO:
 812. 8. The iCP-SOCS3 recombinant protein ofclaim 7, wherein the histidine-tag affinity domain is encoded by thepolynucleotide sequence of SEQ ID NO:
 813. 9. The iCP-SOCS3 recombinantprotein according to claim 1, wherein the iCP-SOCS3 recombinant proteinis used for the treating, preventing, or delaying the onset of,pancreatic cancer.
 10. A preparing method of the iCP-SOCS3 recombinantprotein of claim 1 comprising: preparing a recombinant expression vectorcomprising a polynucleotide sequence encoding the iCP-SOCS3 recombinantprotein of claim 1; preparing a transformant using the recombinantexpression vector; culturing the transformant; and recovering therecombinant protein expressed by the culturing.
 11. A method oftreating, preventing, or delaying the onset of, pancreatic cancer in asubject comprising: identifying a subject in need of treating,preventing, or delaying the onset of, pancreatic cancer; andadministering to the subject a therapeutically effective amount of theiCP-SOCS3 recombinant protein of claim 1.